EY666052 | Citrus Genome Database

Overview


Name	EY666052
Unique Name	EY666052
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	854

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: GRP75_MOUSE (Stress-70 protein, mitochondrial OS=Mus musculus GN=Hspa9 PE=1 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 3.274e-51
Identity = 68/93 (73.12%), Postives = 77/93 (82.80%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNA 540
            V+G+DLGTTNSCVAVMEGK  KV+EN+EG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRR++D + QK+ K VPFKIV A
Sbjct:   55 VVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDTKNVPFKIVRA 147

HSP 2 Score: 83.1889 bits (204), Expect = 3.274e-51
Identity = 44/71 (61.97%), Postives = 53/71 (74.65%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANE--GCWEKLPVLKV 751
            NGDAWVEA+G+ YSPSQIGAFVL KMKETAE+YLG +   AVI VP  F D+Q++A +  G    L VL+V
Sbjct:  149 NGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRV 219

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: GRP75_CRIGR (Stress-70 protein, mitochondrial OS=Cricetulus griseus GN=HSPA9 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 3.274e-51
Identity = 68/93 (73.12%), Postives = 77/93 (82.80%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNA 540
            V+G+DLGTTNSCVAVMEGK  KV+EN+EG RTTPSVVAF   GE LVG PAKRQAVTNP NT + TKRLIGRR++D + QK+ K VPFKIV A
Sbjct:   55 VVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDTKNVPFKIVRA 147

HSP 2 Score: 83.1889 bits (204), Expect = 3.274e-51
Identity = 44/71 (61.97%), Postives = 53/71 (74.65%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANE--GCWEKLPVLKV 751
            NGDAWVEA+G+ YSPSQIGAFVL KMKETAE+YLG +   AVI VP  F D+Q++A +  G    L VL+V
Sbjct:  149 NGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRV 219

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_PELUB (Chaperone protein dnaK OS=Pelagibacter ubique GN=dnaK PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 3.275e-51
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNAPK 546
            +IG+DLGTTNSCV++MEG  PKV+EN+EG RTTPSVVAF + GE LVG PAKRQAVTNP NT+F  KRLIGR F D   +K++   PFKIVN+ K
Sbjct:    4 IIGIDLGTTNSCVSIMEGSQPKVLENAEGARTTPSVVAFTEDGEKLVGQPAKRQAVTNPENTIFAVKRLIGRSFEDPTVKKDIAAAPFKIVNSEK 98

HSP 2 Score: 86.2705 bits (212), Expect = 3.275e-51
Identity = 48/103 (46.60%), Postives = 64/103 (62.14%), Query Frame = 2

Query:  542 QNGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            + GDAW+EA G++YSPSQI AF+L KMKETAE YLG+  +KAVI VP  F DAQ++A +    K+  L+V +II       +   G   K    +  +D GGG
Sbjct:   97 EKGDAWIEAKGEKYSPSQISAFILQKMKETAEKYLGQEVTKAVITVPAYFNDAQRQATKDA-GKIAGLEVLRIINEPTA-ASLAYGLDKKQNKKIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_RHILO (Chaperone protein dnaK OS=Rhizobium loti GN=dnaK PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 3.275e-51
Identity = 64/91 (70.33%), Postives = 78/91 (85.71%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIV 534
            VIG+DLGTTNSC+A+M+GK PKVIEN+EG RTTPS+VA +  GE LVG PAKRQAVTNP NT+F  KRLIGRR++D  T+K+ K+VP+KIV
Sbjct:    4 VIGIDLGTTNSCIAIMDGKEPKVIENAEGARTTPSIVAISGDGERLVGQPAKRQAVTNPENTIFAVKRLIGRRYDDPVTEKDKKLVPYKIV 94

HSP 2 Score: 85.1149 bits (209), Expect = 3.275e-51
Identity = 49/102 (48.04%), Postives = 63/102 (61.76%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            NGDAWVEA G++ SPSQI A +L KMKETAE+YLG+   KAVI VP  F DAQ++A +    K+  L+V +II           G   K G ++  +D GGG
Sbjct:   98 NGDAWVEAGGKKQSPSQISAMILQKMKETAEAYLGEKVEKAVITVPAYFNDAQRQATKDA-GKIAGLEVLRIINEPTA-AALAYGLDKKDGKTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_NOVAD (Chaperone protein dnaK OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=dnaK PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.275e-51
Identity = 65/90 (72.22%), Postives = 76/90 (84.44%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKI 531
            VIG+DLGTTNSCVAVM+G  PKVIENSEG RTTPS+VAF + GE L+G PAKRQAVTNP NT+F  KRLIGRRF+D  TQK+ ++VP+ I
Sbjct:    4 VIGIDLGTTNSCVAVMDGGTPKVIENSEGARTTPSIVAFTKDGERLIGQPAKRQAVTNPDNTIFAVKRLIGRRFDDPMTQKDTELVPYTI 93

HSP 2 Score: 85.8853 bits (211), Expect = 3.275e-51
Identity = 48/103 (46.60%), Postives = 66/103 (64.08%), Query Frame = 2

Query:  542 QNGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            +NGDAWV+A GQ YSPSQI AF L KMKETAE+YLG++ ++AVI VP  F DAQ++A +   + +  L+V +II           G   + G ++  +D GGG
Sbjct:   97 KNGDAWVKAGGQDYSPSQISAFTLQKMKETAEAYLGETVTQAVITVPAYFNDAQRQATKDAGQ-IAGLEVLRIINEPTA-AALAYGLDKQDGKTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_MAGSA (Chaperone protein dnaK OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) GN=dnaK PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 4.269e-51
Identity = 67/91 (73.63%), Postives = 76/91 (83.52%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIV 534
            VIG+DLGTTNSCVAVMEGKN KVIEN+EG RTTPS+ AF + GE LVG PAKRQAVTNP +TLF  KRLIGRRF D  T+K+M +VP+ IV
Sbjct:    4 VIGIDLGTTNSCVAVMEGKNAKVIENAEGMRTTPSMTAFTESGERLVGQPAKRQAVTNPTSTLFAIKRLIGRRFEDPITKKDMNLVPYHIV 94

HSP 2 Score: 85.5001 bits (210), Expect = 4.269e-51
Identity = 47/102 (46.08%), Postives = 64/102 (62.75%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            NGDAWVEA   +YSPSQ+ AF+L KMKETAE YLG+  ++AVI VP  F DAQ++A +    ++  L+V +II           G + KG  ++  +D GGG
Sbjct:   98 NGDAWVEARDAKYSPSQVSAFILQKMKETAEGYLGEKVTQAVITVPAYFNDAQRQATKDA-GRIAGLEVLRIINEPTA-AALAYGLEKKGAGTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_RHOCS (Chaperone protein dnaK OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=dnaK PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 4.269e-51
Identity = 65/91 (71.43%), Postives = 75/91 (82.42%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIV 534
            VIG+DLGTTNSCVA+MEG   KVIEN+EG RTTPS+VAF Q GE LVG PAKRQAVTNP NT F  KRLIGRR++D  TQK+  +VP++IV
Sbjct:    4 VIGIDLGTTNSCVAIMEGTQAKVIENAEGARTTPSMVAFTQGGERLVGQPAKRQAVTNPENTFFAIKRLIGRRYDDPLTQKDKGLVPYRIV 94

HSP 2 Score: 88.9669 bits (219), Expect = 4.269e-51
Identity = 48/103 (46.60%), Postives = 69/103 (66.99%), Query Frame = 2

Query:  542 QNGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            +NGDAWVE++G+QYSPS+I AF+L KMKETAE+YLG+  ++AVI VP  F D+Q++A +    K+  L+V +II           G + KG  ++  +D GGG
Sbjct:   97 KNGDAWVESHGKQYSPSEISAFILQKMKETAENYLGEKVTQAVITVPAYFNDSQRQATKDA-GKIAGLEVLRIINEPTA-AALAYGMEKKGTGTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_AZOC5 (Chaperone protein dnaK OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=dnaK PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 4.270e-51
Identity = 63/93 (67.74%), Postives = 76/93 (81.72%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIVNA 540
            +IG+DLGTTNSCVAVMEG +PKVIEN+EG RTTPS+VAF + GE LVG PAKRQ VTNP  T F  KRLIGRR++D   +K+ K+VP+K+V A
Sbjct:    4 IIGIDLGTTNSCVAVMEGASPKVIENAEGARTTPSIVAFTEDGERLVGQPAKRQGVTNPERTFFAVKRLIGRRYDDPTVEKDKKLVPYKVVRA 96

HSP 2 Score: 86.6557 bits (213), Expect = 4.270e-51
Identity = 48/102 (47.06%), Postives = 65/102 (63.73%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            NGDAWVE++G++YSPSQI AF+L KMKETAES+LG+   KAVI VP  F DAQ++A +    ++  L+V +II           G   K   ++  +D GGG
Sbjct:   98 NGDAWVESDGKKYSPSQISAFILQKMKETAESFLGEKVEKAVITVPAYFNDAQRQATKDA-GRIAGLEVLRIINEPTA-AALAYGLDKKNSGTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_BARQU (Chaperone protein dnaK OS=Bartonella quintana GN=dnaK PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.566e-51
Identity = 67/91 (73.63%), Postives = 75/91 (82.42%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIV 534
            VIG+DLGTTNSCVAVM+GKN KVIENSEG RTTPSVVAF   GE LVG PAKRQAVTNP  T+F  KRLIGRRF+D   +K+  +VP+KIV
Sbjct:    4 VIGIDLGTTNSCVAVMDGKNAKVIENSEGARTTPSVVAFTDGGERLVGQPAKRQAVTNPEGTIFAVKRLIGRRFDDPMVEKDKALVPYKIV 94

HSP 2 Score: 85.1149 bits (209), Expect = 5.566e-51
Identity = 49/102 (48.04%), Postives = 63/102 (61.76%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            NGDAWVE  G++YSPSQI A +L KMKETAESYLG+   +AVI VP  F DAQ++A +    K+  L+V +II           G   K G ++  +D GGG
Sbjct:   98 NGDAWVEEAGKKYSPSQISAMILQKMKETAESYLGEKVEQAVITVPAYFNDAQRQATKDA-GKIAGLEVLRIINEPTA-AALAYGLDKKDGKTIAVYDLGGG 197

[Close]

BLAST of EY666052 vs. ExPASy Swiss-Prot
Match: DNAK_BARHE (Chaperone protein dnaK OS=Bartonella henselae GN=dnaK PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.566e-51
Identity = 67/91 (73.63%), Postives = 75/91 (82.42%), Query Frame = 1

Query:  262 VIGVDLGTTNSCVAVMEGKNPKVIENSEGTRTTPSVVAFNQKGELLVGTPAKRQAVTNPANTLFGTKRLIGRRFNDAQTQKEMKMVPFKIV 534
            VIG+DLGTTNSCVAVM+GKN KVIENSEG RTTPSVVAF   GE LVG PAKRQAVTNP  T+F  KRLIGRRF+D   +K+  +VP+KIV
Sbjct:    4 VIGIDLGTTNSCVAVMDGKNAKVIENSEGARTTPSVVAFTDGGERLVGQPAKRQAVTNPEGTIFAVKRLIGRRFDDPMVEKDKALVPYKIV 94

HSP 2 Score: 85.1149 bits (209), Expect = 5.566e-51
Identity = 49/102 (48.04%), Postives = 63/102 (61.76%), Query Frame = 2

Query:  545 NGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSGSKAVILVPTNFYDAQKEANEGCWEKLPVLKVQKIIK*AYCWWTFPMG*KNKGGASLEFFDFGGG 850
            NGDAWVE  G++YSPSQI A +L KMKETAESYLG+   +AVI VP  F DAQ++A +    K+  L+V +II           G   K G ++  +D GGG
Sbjct:   98 NGDAWVEEAGKKYSPSQISAMILQKMKETAESYLGEKVEQAVITVPAYFNDAQRQATKDA-GKIAGLEVLRIINEPTA-AALAYGLDKKDGKTIAVYDLGGG 197

The following BLAST results are available for this feature:

BLAST of EY666052 vs. ExPASy Swiss-Prot

BLAST of EY666052 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
GRP75_MOUSE	3.274e-51	73.12	Stress-70 protein, mitochondrial OS=Mus musculus G...	[more]
GRP75_CRIGR	3.274e-51	73.12	Stress-70 protein, mitochondrial OS=Cricetulus gri...	[more]
DNAK_PELUB	3.275e-51	67.37	Chaperone protein dnaK OS=Pelagibacter ubique GN=d...	[more]
DNAK_RHILO	3.275e-51	70.33	Chaperone protein dnaK OS=Rhizobium loti GN=dnaK P...	[more]
DNAK_NOVAD	3.275e-51	72.22	Chaperone protein dnaK OS=Novosphingobium aromatic...	[more]
DNAK_MAGSA	4.269e-51	73.63	Chaperone protein dnaK OS=Magnetospirillum magneti...	[more]
DNAK_RHOCS	4.269e-51	71.43	Chaperone protein dnaK OS=Rhodospirillum centenum ...	[more]
DNAK_AZOC5	4.270e-51	67.74	Chaperone protein dnaK OS=Azorhizobium caulinodans...	[more]
DNAK_BARQU	5.566e-51	73.63	Chaperone protein dnaK OS=Bartonella quintana GN=d...	[more]
DNAK_BARHE	5.566e-51	73.63	Chaperone protein dnaK OS=Bartonella henselae GN=d...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-013-C10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY666052 ID=EY666052; Name=EY666052; organism=Citrus sinensis; type=EST; length=854bp
CCCACGCGTCCGAACCCTTGGAAAAGCAACCTAAAACGCTACAAGCTTTC
TCTCGCTTTCAAAAGAGCTAAAGCAAATCGATTAGTCGATATTTACTCAA
TGGCCATCGCCGGTATTCTCCGCTCCTTACGACGGCGAGATGTCGCCTCC
GCCCATCTCTCTGCCTGCAAATCCTTGACGTGCAATGTCAAACCCTCCTG
GGCTTCTGCAAATTGGGCGAGTTTGGCAAGACCTTTCAGTGTGAGACCAG
CAGGGAATGATGTTATTGGGGTTGATTTGGGAACCACCAATTCATGTGTT
GCGGTCATGGAAGGAAAGAATCCTAAAGTTATTGAGAATTCTGAAGGCAC
GAGGACCACGCCGTCAGTGGTGGCCTTCAATCAGAAAGGTGAGTTGCTTG
TGGGTACACCAGCAAAACGTCAGGCTGTGACTAACCCAGCTAATACACTT
TTTGGAACTAAGCGTCTGATTGGTAGGCGATTCAATGATGCCCAAACTCA
AAAAGAAATGAAAATGGTTCCATTCAAGATAGTCAATGCTCCAAAACGGA
GATGCTTGGGTCGAGGCCAATGGGCAACAGTACTCCCCAAGCCAGATCGG
AGCATTCGTTCTAACCAAGATGAAAGAAACAGCAGAGTCTTATCTTGGAA
AGTCTGGTTCTAAAGCAGTGATCCTTGTACCAACTAATTTTTATGATGCT
CAGAAGGAAGCAAACGAAGGATGCTGGGAAAAATTGCCGGTCCTCAAGGT
CCAAAAAATTATTAAATGAGCCTACTGCTGGTGGACTTTTCCTATGGGAT
GAAAAAACAAAGGGGGGGCCTCATTGGAGTTTTTTGATTTTGGGGGGGGG
GAAC