CN188428

Overview
NameCN188428
Unique NameCN188428
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length461
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_POLSJ (Malate dehydrogenase OS=Polaromonas sp. (strain JS666 / ATCC BAA-500) GN=mdh PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 7.863e-29
Identity = 65/149 (43.62%), Postives = 87/149 (58.39%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVP 448
            P   N  I  + AP +P K FT++ RLDHNRA  QI+ K    V+D++ + +WGNHS + Y D   AT+N   GE  V +++ D  W    F+ TV +RGAAII+AR             DH+RDW LGT  G WV+MG+ SDG Y +P
Sbjct:  135 PANTNAYIAMKSAPDLPRKNFTAMLRLDHNRAASQIAAKTGKAVADIEKLTVWGNHSPTMYADYRFATIN---GES-VAKMINDQEWNANVFLPTVGKRGAAIIEARGLSSAASAANAAIDHMRDWALGT-NGKWVTMGIPSDGQYGIP 278          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_LARHH (Malate dehydrogenase OS=Laribacter hongkongensis (strain HLHK9) GN=mdh PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 7.863e-29
Identity = 63/132 (47.73%), Postives = 85/132 (64.39%), Query Frame = 2
Query:   62 FTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL 457
            +T++ RLDHNRAL Q++ K    V+ ++N+ +WGNHS + Y D   ATVN   GE  V+ ++ D+AW    F+ TV +RGAAII+AR             DHIRDWVLGT  G WV+MG+ SDGSY +P G+
Sbjct:  155 YTAMLRLDHNRALSQLAAKTGKAVASIENMAVWGNHSPTMYADYRFATVN---GES-VKSMINDEAWNRDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGT-NGKWVTMGIPSDGSYGIPEGV 281          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_VARPS (Malate dehydrogenase OS=Variovorax paradoxus (strain S110) GN=mdh PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.027e-28
Identity = 64/149 (42.95%), Postives = 87/149 (58.39%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVP 448
            P   N  I  + AP +P K FT++ RLDHNRA  QI+ K    V+D++ +++WGNHS + Y D   AT+    GE  V +++ D  W    F+ TV +RGAAII+AR             DH+RDW LGT  G WV+MG+ SDG Y +P
Sbjct:  135 PANTNAYIAMKSAPDLPRKNFTAMLRLDHNRAASQIAAKTGKAVADIEKLVVWGNHSPTMYADYRFATIK---GES-VAKMINDQEWNANTFLPTVGKRGAAIIEARGLSSAASAANAAIDHMRDWALGT-NGKWVTMGIPSDGQYGIP 278          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_CORDI (Malate dehydrogenase OS=Corynebacterium diphtheriae GN=mdh PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.027e-28
Identity = 65/153 (42.48%), Postives = 89/153 (58.17%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LI +  A  IPA  F ++ RLDHNR + Q+SEKL    +D++  ++WGNHS+ Q+PD+ +AT+    G + +  LV  D W  GEFI  V +RGA II+ R             DH+ DW+ GT +G W +  + SDGSY VP GLI
Sbjct:  133 PANTNALIAQHAAKDIPADRFNAMMRLDHNRGIAQLSEKLGRDKNDIEKFVVWGNHSAGQFPDITYATI----GGEAISGLVDHD-WYTGEFIPRVAKRGAEIIEVRGKSSAASAASSAIDHMHDWINGT-DGQWRTAAIPSDGSYGVPEGLI 279          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_BDEBA (Malate dehydrogenase OS=Bdellovibrio bacteriovorus GN=mdh PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.027e-28
Identity = 69/143 (48.25%), Postives = 87/143 (60.84%), Query Frame = 2
Query:   47 IPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVK-----DDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            + AK FT++ RLDHNRAL Q++ K    V+  K V +WGNHS + YPDV  AT + A     V EL+K      DAW    FI TV +RGAAII+AR             DHIRDW LGT  G WV+MG+ SDGSY++P G++
Sbjct:  152 VKAKNFTAMLRLDHNRALSQLATKTGKPVASFKKVAVWGNHSPTMYPDVRFATADGA----KVPELLKLGTAEGDAWNKDTFIPTVGKRGAAIIEARGLSSAASAASAAVDHIRDWWLGT-NGEWVTMGIPSDGSYDIPEGIM 289          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_RALEJ (Malate dehydrogenase OS=Ralstonia eutropha (strain JMP134) GN=mdh PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.341e-28
Identity = 64/150 (42.67%), Postives = 90/150 (60.00%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPA 451
            P   N  I  + APS+P + FT++ RLDHNRAL QI+ K    V+ ++ + +WGNHS + Y D  +ATV+     K V++++ D  W N  F+ TV +RGAAII+AR             DH+ DWVLGT  G  V+MG+ S+G Y +PA
Sbjct:  133 PANTNAYIAMKSAPSLPRENFTAMLRLDHNRALSQIAAKTGKPVASIEKMFVWGNHSPTMYADYRYATVD----GKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHVHDWVLGT-NGKVVTMGIPSNGEYGIPA 277          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_XANOP (Malate dehydrogenase OS=Xanthomonas oryzae pv. oryzae (strain PXO99A) GN=mdh PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.752e-28
Identity = 65/153 (42.48%), Postives = 89/153 (58.17%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N  I  + AP +    FT++ RLDHNRAL Q++ KL   V  ++ +++WGNHS + YPD   AT + A+    + + + D  W    FI TV +RGAAII+AR             DH+RDWVLG+  G WV+MGV SDGSY +  G+I
Sbjct:  134 PANTNAYIAMKSAPDLNPNNFTAMLRLDHNRALSQLALKLGKPVGGIEKLVVWGNHSPTMYPDYRFATSDGAS----IGDAINDQEWNASTFIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGS-NGKWVTMGVPSDGSYGISEGVI 281          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_RALPJ (Malate dehydrogenase OS=Ralstonia pickettii (strain 12J) GN=mdh PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.752e-28
Identity = 63/149 (42.28%), Postives = 89/149 (59.73%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVP 448
            P   N  I  + AP +P + FT++ RLDHNRAL QI+ K    VS ++ + +WGNHS + Y D  +AT++     K V++++ D  W N  F+ TV +RGAAII+AR             DH+RDWVLGT  G  V+MG+ S+G Y +P
Sbjct:  135 PANTNAYIAMKSAPDLPRENFTAMLRLDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATID----GKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKIVTMGIPSNGEYGIP 278          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_CUPTR (Malate dehydrogenase OS=Cupriavidus taiwanensis (strain R1 / LMG 19424) GN=mdh PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.752e-28
Identity = 64/150 (42.67%), Postives = 91/150 (60.67%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPA 451
            P   N  I  + AP++P + FT++ RLDHNRAL QI+ K    VS ++ + +WGNHS + Y D  +ATV+     + V++L+ D  W N  F+ TV +RGAAII+AR             DH+RDWVLG+  G  V+MG+ S+G Y +PA
Sbjct:  133 PANTNAYIAMKSAPNLPRENFTAMLRLDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATVD----GQSVKDLINDPVWNNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGS-NGKVVTMGIPSNGEYGIPA 277          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDH_ACICJ (Malate dehydrogenase OS=Acidiphilium cryptum (strain JF-5) GN=mdh PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.752e-28
Identity = 63/153 (41.18%), Postives = 88/153 (57.52%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N  I  + AP +    F+++ RLDHNRA   ++ K  V V DV  +I+WGNHS + YPD   A     AG + + E + D+AW    FI TV +RGAA+I+AR             D +RDWV+G+ +G WVSM + SDGSY +P G++
Sbjct:  133 PANTNAYIAMKSAPDLSPDCFSAMIRLDHNRAASMLAAKAGVNVGDVGKLIVWGNHSPTMYPDYRFA----EAGGRKLAEAINDEAWNRDVFIPTVGKRGAAVIEARGASSAASAANAAIDQVRDWVVGS-DGRWVSMAIPSDGSYGIPEGIM 280          
The following BLAST results are available for this feature:
BLAST of CN188428 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 187
Match NameE-valueIdentityDescription
MDH_POLSJ7.863e-2943.62Malate dehydrogenase OS=Polaromonas sp. (strain JS... [more]
MDH_LARHH7.863e-2947.73Malate dehydrogenase OS=Laribacter hongkongensis (... [more]
MDH_VARPS1.027e-2842.95Malate dehydrogenase OS=Variovorax paradoxus (stra... [more]
MDH_CORDI1.027e-2842.48Malate dehydrogenase OS=Corynebacterium diphtheria... [more]
MDH_BDEBA1.027e-2848.25Malate dehydrogenase OS=Bdellovibrio bacteriovorus... [more]
MDH_RALEJ1.341e-2842.67Malate dehydrogenase OS=Ralstonia eutropha (strain... [more]
MDH_XANOP1.752e-2842.48Malate dehydrogenase OS=Xanthomonas oryzae pv. ory... [more]
MDH_RALPJ1.752e-2842.28Malate dehydrogenase OS=Ralstonia pickettii (strai... [more]
MDH_CUPTR1.752e-2842.67Malate dehydrogenase OS=Cupriavidus taiwanensis (s... [more]
MDH_ACICJ1.752e-2841.18Malate dehydrogenase OS=Acidiphilium cryptum (stra... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006A09_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006A09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188428 ID=CN188428; Name=CN188428; organism=Citrus sinensis; type=EST; length=461bp
CCCTGNNATTATNAATGNATTGATTCTGAAAGAATTTGCGCCATCAATCC
CTGCTAAGATCTTCACAAGCTTGACCAGATTGGATCATAACAGGGCATTG
GGACAAATATCTGAGAAACTGAATGTCCAAGTATCTGATGTTAAGAATGT
GATTATTTGGGGAAATCATTCATCATCTCAGTACCCTGATGTCAACCATG
CAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGCTTGTCAAG
GATGATGCATGGTTGAATGGAGAATTCATAACCACAGTCCAACAACGTGG
TGCTGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGTCTGCTGCTA
GCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACTCCTGAGGGC
ACTTGGGTCTCCATGGGGGTATACTCTGATGGTTCATACAATGTACCAGC
TGGGCTCATTT
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