CK939415

Overview
NameCK939415
Unique NameCK939415
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length865
Libraries
Library NameType
Developing fruit flavedo at 165 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GST16_ARATH (Glutathione S-transferase 16 OS=Arabidopsis thaliana GN=GST16 PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.601e-39
Identity = 86/210 (40.95%), Postives = 130/210 (61.90%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELL---GKTIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVL 685
            +KV+G PA  S +RV++ L EK ++FE V V+L  GE +   +L   PFG++P  +DGD  L+ESRAI +Y A +Y +QGT LL    K I +  ++   ++VEAH ++P   +L  + +F   +G   D+ +V E + KL K LDVYE RL + KYLAG+ F+L DL HIP   YL+G   ++ +  +   V+ W  +I+ RP+ +KVL
Sbjct:    4 IKVFGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNLLPADSKNIAQYAIMSIGIQVEAHQFDPVASKLAWEQVFKFNYGLNTDQAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPVIQYLLGTPTKK-LFTERPRVNEWVAEITKRPASEKVL 212          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF4_ARATH (Glutathione S-transferase PM24 OS=Arabidopsis thaliana GN=PM24.1 PE=1 SV=3)

HSP 1 Score: 161.77 bits (408), Expect = 4.658e-39
Identity = 85/209 (40.67%), Postives = 129/209 (61.72%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELL---GKTIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKV 682
            +KV+G PA  + +RV++ L EK ++FE V V+L  GE +   +L   PFG++P  +DGD  L+ESRAI +Y A +Y +QGT LL    K I +  ++   ++VE H ++P   +L  + +F S +G   DE +V E + KL K LDVYE RL + KYLAG+ F+L DL HIP   YL+G   ++ +  +   V+ W  +I+ RP+ +KV
Sbjct:    4 IKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNLLQTDSKNISQYAIMAIGMQVEDHQFDPVASKLAFEQIFKSIYGLTTDEAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPAIQYLLGTPTKK-LFTERPRVNEWVAEITKRPASEKV 211          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF1_ARATH (Glutathione S-transferase 1 OS=Arabidopsis thaliana GN=GST1 PE=1 SV=2)

HSP 1 Score: 160.229 bits (404), Expect = 1.355e-38
Identity = 83/207 (40.10%), Postives = 131/207 (63.29%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELLGKTIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVL 685
            +KV+G PA  + +RV++ L EK ++FE V V+L  GE +   ++   PFG++P  +DGDF ++ESRAI +Y A ++  +G  LL  T ++  ++   +E+E+H ++P   +L  + +    +G   D+ +V+E + KL K LDVYE RL +SKYLA D F+L DL  IP   YL+G   ++ +  +  HVSAW  DI++RPS +KVL
Sbjct:    4 IKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIFESRAITQYIAHEFSDKGNNLLS-TGKDMAIIAMGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDLHTIPVIQYLLGTPTKK-LFDERPHVSAWVADITSRPSAQKVL 208          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF1_MAIZE (Glutathione S-transferase 1 OS=Zea mays GN=GST1 PE=1 SV=4)

HSP 1 Score: 159.844 bits (403), Expect = 1.770e-38
Identity = 90/212 (42.45%), Postives = 127/212 (59.91%), Query Frame = 2
Query:   68 VKVYGPAYA-SPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELLGK-TIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVLELCK 697
            +K+YG   + +  R    L E   ++E VP++    E +SPE+L   PFG++P +QDGD  L+ESRAI +Y A K +    ELL +  +EE  +V+ W+EVEA+ Y  A+  +  Q+L S   G   D+K+V E+ EKL K L+VYE RL+K KYLAGDF SLADL+H+  T  L        ++  + HV AWW  +  RPS +KV  L K
Sbjct:    4 MKLYGAVMSWNLTRCATALEEAGSDYEIVPINFATAEHKSPEHLVRNPFGQVPALQDGDLYLFESRAICKYAARKNKP---ELLREGNLEEAAMVDVWIEVEANQYTAALNPILFQVLISPMLGGTTDQKVVDENLEKLKKVLEVYEARLTKCKYLAGDFLSLADLNHVSVTLCLFA-TPYASVLDAYPHVKAWWSGLMERPSVQKVAALMK 211          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GST11_ARATH (Glutathione S-transferase 11 OS=Arabidopsis thaliana GN=GST11 PE=1 SV=3)

HSP 1 Score: 159.073 bits (401), Expect = 3.019e-38
Identity = 83/207 (40.10%), Postives = 129/207 (62.32%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELLGKTIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVL 685
            +KV+G PA  + +RV++ L EK ++FE V ++L  GE +   ++   PFG++P  +DGDF L+ESRAI +Y A  Y  +G +L+    ++   +   +E+E+H ++P   +L  + +    +G   D+ +V+E + KL K LDVYE RL +SKYLA D F+L DL  IP   YL+G   ++ +  +  HVSAW  DI++RPS KKVL
Sbjct:    4 IKVFGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFEDGDFKLFESRAITQYIAHFYSDKGNQLVSLGSKDIAGIAMGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDLHTIPVIQYLLGTPTKK-LFDERPHVSAWVADITSRPSAKKVL 209          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF4_MAIZE (Glutathione S-transferase 4 OS=Zea mays GN=GST4 PE=1 SV=2)

HSP 1 Score: 158.303 bits (399), Expect = 5.150e-38
Identity = 89/213 (41.78%), Postives = 128/213 (60.09%), Query Frame = 2
Query:   56 ATMVVKVYGPAYAS-PKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELLGK-TIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVLE 688
            AT  VKVYG A +    R +L L E  +++E VP+    G+ R PE+L   PFG++PV++DGD  L+ESRAI R+   K++    ELLG   +E+  +V+ WLEVEAH  +P    + ++ +F+   GR  ++ +V E+ EKL K L+VYE RL+   YLAGDF SLADLS     H L+       ++    HVSAWW  ++ RP+  KV +
Sbjct:    2 ATPAVKVYGWAISPFVSRALLALEEAGVDYELVPMSRQDGDHRRPEHLARNPFGKVPVLEDGDLTLFESRAIARHVLRKHKP---ELLGGGRLEQTAMVDVWLEVEAHQLSPPAIAIVVECVFAPFLGRERNQAVVDENVEKLKKVLEVYEARLATCTYLAGDFLSLADLSPFTIMHCLMA-TEYAALVHALPHVSAWWQGLAARPAANKVAQ 210          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF1_WHEAT (Glutathione S-transferase 1 OS=Triticum aestivum GN=GSTA1 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.147e-37
Identity = 92/222 (41.44%), Postives = 132/222 (59.46%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKY-RSQGTELLGKT--IEERGLVEQWLEVEAHNYNPAI----YELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHY-LVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVLELCKTPF 706
            VKV+G P   +  RV+L L E   E+E VP+D + GE + P++++L PF ++P  QDGD +L+ESRAI +Y   KY  + G +LLG+   IEE  +V+ W EVEA  Y PAI    +E  I        G   ++ +V ES E+L   L +YE RL KS+YLAGD  + ADL+HIPFT Y +  P  +  +  D+  V AWW+ +  RP+ ++V +   T F
Sbjct:    4 VKVFGHPMLTNVARVLLFLEEVGAEYELVPMDFVAGEHKRPQHVQLNPFAKMPGFQDGDLVLFESRAIAKYILRKYGGTAGLDLLGENSGIEELAMVDVWTEVEAQQYYPAISPVVFECIIIPFIIPGGGAAPNQTVVDESLERLRGVLGIYEARLEKSRYLAGDSITFADLNHIPFTFYFMTTPYAK--VFDDYPKVKAWWEMLMARPAVQRVCKHMPTEF 223          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF2_WHEAT (Glutathione S-transferase 2 OS=Triticum aestivum GN=GSTA2 PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 2.392e-35
Identity = 90/222 (40.54%), Postives = 130/222 (58.56%), Query Frame = 2
Query:   68 VKVYG-PAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKY-RSQGTELLGKT--IEERGLVEQWLEVEAHNYNPAI----YELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHY-LVGPMGRQHMIRDWKHVSAWWDDISNRPSWKKVLELCKTPF 706
            VKV+G P   +  RV+L L E   E+E VPVD + GE + P++++L PF ++P  QDG+ +  +SRAI +Y   KY  + G +LLG+   IEE  +V+ W EVEA  Y PAI    +E  I        G   ++ +V ES E+L   L +YE RL KS+YLAGD  S ADL+HIPFT Y +  P  +  +  ++  V AWW+ +  RP+ ++V +   T F
Sbjct:    4 VKVFGHPMLTNVARVLLFLEEVGAEYELVPVDFVAGEHKRPQHVQLNPFAKMPGFQDGESLHIKSRAIAKYILRKYGGTAGLDLLGENSGIEELAMVDVWTEVEAQQYYPAISPVVFECIIIPFIIPGGGAAPNQTVVDESLERLRGVLGIYEARLEKSRYLAGDSISFADLNHIPFTFYFMTTPYAK--VFDEYPKVKAWWEMLMARPAVQRVCKHMPTEF 223          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GSTF5_ARATH (Glutathione S-transferase PM239X14 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 4.831e-28
Identity = 81/203 (39.90%), Postives = 115/203 (56.65%), Query Frame = 2
Query:   62 MVVKVYGPAYAS-PKRVILCLVEKEIEFETVPVDLIKGEQRSPEYL-KLQPFGELPVIQDGDFI-LYESRAIMRYYAEKYRSQGTELLGKTIEER--GLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIPFTHYLVGPMGRQ---HMIRDWKHVSAWW 646
            + VK+YG AY++  KRV     E  ++ + VPVDL+KGE + P YL    PFG +PV++D D   +YESRAI RY   KY  +G+ LL    + +  GL EQ   VE  +++P    L  + +F+   G   +E+L K+  + L   +D YE  LSK KYLAG+ F+LADL H+P+     G M  Q    ++    HV AWW
Sbjct:    2 VTVKLYGMAYSTCTKRVYTTAKEIGVDVKIVPVDLMKGEHKEPAYLDNYHPFGVIPVLEDEDGTKIYESRAISRYLVAKY-GKGSSLLPSPSDPKAYGLFEQAASVEYSSFDPPASSLAYERVFAGMRGLKTNEELAKKYVDTLNAKMDGYERILSKQKYLAGNDFTLADLFHLPY-----GAMVAQLEPTVLDSKPHVKAWW 198          
BLAST of CK939415 vs. ExPASy Swiss-Prot
Match: GST1C_ANOGA (Glutathione S-transferase 1, isoform C OS=Anopheles gambiae GN=GstD1 PE=2 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 5.185e-14
Identity = 57/195 (29.23%), Postives = 96/195 (49.23%), Query Frame = 2
Query:   77 YGPAYASPKRVILCLVEKEIEFETVPVDLIKGEQRSPEYLKLQPFGELPVIQDGDFILYESRAIMRYYAEKYRSQGTELLGKTIEERGLVEQWLEVEAHNYNPAIYELTIQLLFSSKFGRPVDEKLVKESDEKLGKTLDVYEERLSKSKYLAGDFFSLADLSHIP--FTHYLVGPMGRQHMIRDWKHVSAWWDDI 655
            Y P  A  + V +      +E      DL+KGE   PE+LK+ P   +P + D  F L+ESRAI  Y AEKY  +  +L  K  ++R +V Q +  +         +     +F+ +   P +E+ +K++   L   LD +       KY+AGD  ++ADLS +    T+ + G       +  ++HV+AW+++I
Sbjct:    5 YLPGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKINPQHCIPTLVDNGFALWESRAICTYLAEKY-GKDDKLYPKDPQKRAVVNQRMYFDMGTLYQRFADYYYPQIFAKQPANPENEQKMKDAVGFLNSFLDGH-------KYVAGDSLTIADLSILATISTYDVAG-----FDLAKYQHVAAWYENI 186          
The following BLAST results are available for this feature:
BLAST of CK939415 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34
Match NameE-valueIdentityDescription
GST16_ARATH1.601e-3940.95Glutathione S-transferase 16 OS=Arabidopsis thalia... [more]
GSTF4_ARATH4.658e-3940.67Glutathione S-transferase PM24 OS=Arabidopsis thal... [more]
GSTF1_ARATH1.355e-3840.10Glutathione S-transferase 1 OS=Arabidopsis thalian... [more]
GSTF1_MAIZE1.770e-3842.45Glutathione S-transferase 1 OS=Zea mays GN=GST1 PE... [more]
GST11_ARATH3.019e-3840.10Glutathione S-transferase 11 OS=Arabidopsis thalia... [more]
GSTF4_MAIZE5.150e-3841.78Glutathione S-transferase 4 OS=Zea mays GN=GST4 PE... [more]
GSTF1_WHEAT1.147e-3741.44Glutathione S-transferase 1 OS=Triticum aestivum G... [more]
GSTF2_WHEAT2.392e-3540.54Glutathione S-transferase 2 OS=Triticum aestivum G... [more]
GSTF5_ARATH4.831e-2839.90Glutathione S-transferase PM239X14 OS=Arabidopsis ... [more]
GST1C_ANOGA5.185e-1429.23Glutathione S-transferase 1, isoform C OS=Anophele... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004750_H09 Developing fruit flavedo at 165 DAFB Citrus sinensis cDNA clone F1650001_IIF_H09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK939415 ID=CK939415; Name=CK939415; organism=Citrus sinensis; type=EST; length=865bp
GGGGACGATCACAAGCTCACTTGCTGCTTTTTATCGACAGAAAAAGTTAA
CACCAGCAACGATGGTGGTCAAGGTGTACGGTCCGGCTTATGCTTCTCCC
AAACGAGTGATTTTGTGCTTGGTTGAGAAGGAAATCGAATTTGAAACTGT
CCCCGTTGATCTCATCAAAGGAGAGCAGAGAAGTCCTGAGTACCTCAAAT
TACAGCCTTTTGGAGAGCTTCCTGTCATTCAAGATGGAGATTTTATTTTA
TACGAGTCGCGAGCTATCATGAGGTACTACGCTGAAAAGTACAGGTCTCA
AGGAACCGAACTTCTGGGTAAAACAATTGAAGAAAGAGGCCTGGTGGAAC
AGTGGCTAGAAGTAGAGGCACACAACTACAACCCAGCAATTTACGAGCTG
ACCATCCAACTTTTATTTTCCTCCAAGTTTGGACGTCCCGTTGATGAAAA
ATTGGTGAAGGAGAGTGACGAAAAGCTTGGAAAAACGCTCGACGTTTACG
AGGAGAGGCTGTCGAAGAGCAAGTATTTGGCTGGTGATTTCTTCAGCCTG
GCTGATCTGAGTCACATTCCATTTACCCACTACTTGGTTGGGCCGATGGG
GAGACAGCATATGATTAGAGACTGGAAGCATGTGAGTGCCTGGTGGGATG
ATATTAGCAACAGGCCGTCCTGGAAGAAAGTCCTTGAGCTCTGTAAGACT
CCTTTCGGCTGAGCTTGATTGTATGTGTTTCAATTTTCGGGCTTGTGTTT
TACTTCTCTACTACTTGGTTTGTTTCTGTCTGTGGGATATGTTTCTTNNN
NNTGTGTGTTTCAAATAAATAAATGTTTGGAGTTGGAGCTTAAAAAAAAA
AAAAAAAAAAAAAAA
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