CK939304

Overview
NameCK939304
Unique NameCK939304
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length803
Libraries
Library NameType
Developing fruit flavedo at 165 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2173 contig Csv1_Contig2173:1..803. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK939304 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.607e-17
Identity = 54/153 (35.29%), Postives = 83/153 (54.25%), Query Frame = 3 
Query:  207 TMFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFLGYEDTASAPADPVDRTVWTFGKPATIELTHNWGTESDPDFKGYHNGNSEPRGFGHIGITVDDVYKACERFERLGVEFAKKPDGGKMKG----VAFIKDPDDYWIEIFDLKTIGK 653
            TM R+ D + S+DFY++VLGM LL+  + PE K+SL F+GY                   + A IELT+NWG +       Y  G +    +GHI ++VD+  +ACE+  + G    +  + G +KG    +AF++DPD Y IE+ + K  G+
Sbjct:    6 TMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPET---------------EEAVIELTYNWGVDK------YELGTA----YGHIALSVDNAAEACEKIRQNGGNVTR--EAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGR 131
[Close]
BLAST of CK939304 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.607e-17
Identity = 54/153 (35.29%), Postives = 83/153 (54.25%), Query Frame = 3 
Query:  207 TMFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFLGYEDTASAPADPVDRTVWTFGKPATIELTHNWGTESDPDFKGYHNGNSEPRGFGHIGITVDDVYKACERFERLGVEFAKKPDGGKMKG----VAFIKDPDDYWIEIFDLKTIGK 653
            TM R+ D + S+DFY++VLGM LL+  + PE K+SL F+GY                   + A IELT+NWG +       Y  G +    +GHI ++VD+  +ACE+  + G    +  + G +KG    +AF++DPD Y IE+ + K  G+
Sbjct:    6 TMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPET---------------EEAVIELTYNWGVDK------YELGTA----YGHIALSVDNAAEACEKIRQNGGNVTR--EAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGR 131
[Close]
BLAST of CK939304 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.607e-17
Identity = 54/153 (35.29%), Postives = 83/153 (54.25%), Query Frame = 3 
Query:  207 TMFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFLGYEDTASAPADPVDRTVWTFGKPATIELTHNWGTESDPDFKGYHNGNSEPRGFGHIGITVDDVYKACERFERLGVEFAKKPDGGKMKG----VAFIKDPDDYWIEIFDLKTIGK 653
            TM R+ D + S+DFY++VLGM LL+  + PE K+SL F+GY                   + A IELT+NWG +       Y  G +    +GHI ++VD+  +ACE+  + G    +  + G +KG    +AF++DPD Y IE+ + K  G+
Sbjct:    6 TMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPET---------------EEAVIELTYNWGVDK------YELGTA----YGHIALSVDNAAEACEKIRQNGGNVTR--EAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGR 131
[Close]
BLAST of CK939304 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 3.187e-15
Identity = 54/157 (34.39%), Postives = 78/157 (49.68%), Query Frame = 3 
Query:  183 TNGYFMQQTMFRIKDPKVSLDFYSRVLGMSLLKRLDFPEMKFSLYFLGYEDTASAPADPVDRTVWTFGKPATIELTHNWGTESDPDFKGYHNGNSEPRGFGHIGITVDDVYKACERFERLGVEFAKKPDGGKMKG----VAFIKDPDDYWIEIFDLK 641
            +NG  +  TM R+ D   S+ FY+ V+GM LL+  +  E +++L F+GY D +                 A IELT+NWG         Y  G +    FGHI I VDD+Y  C+  +  G    +  + G +KG    +AF+KDPD Y IE+   K
Sbjct:    2 SNGRILH-TMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQG---------------AVIELTYNWGKTE------YDLGTA----FGHIAIGVDDIYATCDAIKAAGGNVTR--EAGPVKGGTTHIAFVKDPDGYMIELIQNK 130
The following BLAST results are available for this feature:
BLAST of CK939304 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24
Match NameE-valueIdentityDescription
LGUL_SHIFL2.607e-1735.29Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI2.607e-1735.29Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO572.607e-1735.29Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_VIBPA3.187e-1534.39Probable lactoylglutathione lyase OS=Vibrio paraha... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004752_D05 Developing fruit flavedo at 165 DAFB Citrus sinensis cDNA clone F1650001_IVF_D05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK939304 ID=CK939304; Name=CK939304; organism=Citrus sinensis; type=EST; length=803bp
GGGGATCGTCAACTCGACCTCTATTTCTATCTCCCTCTTCACTTCCATTC
TTTAGCTCCACTAAAACTACAACTAGTTGGTTCAAGGCTTCGAATCGATT
TCGGTTGCTTTCAATGGCTTCCTCGGAGCCCAAAGAATCCCCCGCTAACA
ATCCGGGTCTTCACACCGCCCGTGATGAAGCTACCAACGGATACTTCATG
CAACAAACGATGTTTCGAATTAAGGACCCCAAAGTTAGTCTTGATTTCTA
TTCTCGTGTGTTGGGCATGTCGCTACTAAAGAGGTTGGATTTTCCAGAAA
TGAAATTTAGCCTATACTTTTTGGGCTATGAGGATACTGCATCAGCTCCA
GCTGACCCTGTTGATAGAACTGTTTGGACTTTTGGGAAGCCGGCTACAAT
TGAGCTGACTCATAACTGGGGAACAGAAAGTGATCCCGACTTCAAAGGTT
ATCACAATGGGAATTCAGAACCCCGTGGCTTTGGGCACATCGGCATCACT
GTTGATGATGTATATAAGGCATGTGAGAGGTTTGAACGTCTAGGGGTGGA
ATTTGCAAAGAAACCAGATGGTGGAAAAATGAAAGGCGTAGCATTCATTA
AAGACCCTGATGACTATTGGATTGAAATCTTTGACCTCAAAACAATTGGA
AAAATTGGCGGTAGCACTTGATATGACATTGCTCACTCAGGGAAGGCGGG
ATCCATTCATTGTAGTGTTTGATAAATTCTCTGTTGAATAGAACGTCTTC
CATGAGATGCATTATGGACTGAAATACGATCGCAAATATGTTTCAAACTC
CGA
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