CK937547

Overview
NameCK937547
Unique NameCK937547
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length697
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 1.836e-66
Identity = 118/149 (79.19%), Postives = 134/149 (89.93%), Query Frame = 2 
Query:  203 MASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYAMG 646
            MASKRI +EL+D+Q+ PP +CSAGPVAE D+FHWQATIMGP DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK DK++YESTA+ WTQKYAMG
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 4.090e-66
Identity = 111/147 (75.51%), Postives = 132/147 (89.80%), Query Frame = 2 
Query:  203 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYAM 643
            M+ KRI KEL DL +DPP+SCSAGPV +D++HWQA+IMGP DSPYAGGVF ++IHFP DYP KPPK+A  TK++HPNINSNG+ICLDILK+QWSPALTISKVLLSICSLLTD NPDDPLVPEIAH+YK D+ +YE+TA+ WT+KYA+
Sbjct:    1 MSLKRINKELSDLGRDPPSSCSAGPVGDDLYHWQASIMGPPDSPYAGGVFFLSIHFPTDYPLKPPKIALTTKIYHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHIYKQDRKKYEATAKEWTKKYAV 147
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 4.090e-66
Identity = 114/147 (77.55%), Postives = 127/147 (86.39%), Query Frame = 2 
Query:  203 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYAM 643
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSN SICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ +Y   +R WTQKYAM
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.244e-64
Identity = 112/147 (76.19%), Postives = 125/147 (85.03%), Query Frame = 2 
Query:  203 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYAM 643
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP+DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ +Y  TAR WTQKYAM
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.244e-64
Identity = 112/147 (76.19%), Postives = 125/147 (85.03%), Query Frame = 2 
Query:  203 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYAM 643
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP+DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ +Y  TAR WTQKYAM
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.009e-50
Identity = 90/145 (62.07%), Postives = 114/145 (78.62%), Query Frame = 2 
Query:  206 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYA 640
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++AE++  AR WT++YA
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.009e-50
Identity = 90/145 (62.07%), Postives = 114/145 (78.62%), Query Frame = 2 
Query:  206 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYA 640
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++AE++  AR WT++YA
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.009e-50
Identity = 90/145 (62.07%), Postives = 114/145 (78.62%), Query Frame = 2 
Query:  206 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYA 640
            ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++AE++  AR WT++YA
Sbjct:   48 SAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.009e-50
Identity = 90/145 (62.07%), Postives = 114/145 (78.62%), Query Frame = 2 
Query:  206 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYA 640
            ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++AE++  AR WT++YA
Sbjct:   48 SAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
[Close]
BLAST of CK937547 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.663e-49
Identity = 89/145 (61.38%), Postives = 112/145 (77.24%), Query Frame = 2 
Query:  206 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAEYESTARSWTQKYA 640
            ++KRI KEL ++  DPP +CSAGP  ++++ W +TI+GP  S Y GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y  ++ E++  AR WT++YA
Sbjct:   87 SAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIARLWTKRYA 231
The following BLAST results are available for this feature:
BLAST of CK937547 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 268
Match NameE-valueIdentityDescription
UBC12_ARATH1.836e-6679.19Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBC4_CANAL4.090e-6675.51Ubiquitin-conjugating enzyme E2 4 OS=Candida albic... [more]
UB2D3_BOVIN4.090e-6677.55Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
U2D2B_RAT2.244e-6476.19Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE2.244e-6476.19Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2E2_MOUSE7.009e-5062.07Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN7.009e-5062.07Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UB2E1_MOUSE7.009e-5062.07Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN7.009e-5062.07Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UBCD2_DROME2.663e-4961.38Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCGF1004779_D03 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_D03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937547 ID=CK937547; Name=CK937547; organism=Citrus sinensis; type=EST; length=697bp
GGGGCTCGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAG
AGGAGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCG
CTTTCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGTTTC
GCAGGTCTTAAGGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAG
TCATGGCTTCAAAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGAT
CCTCCCACGTCTTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTG
GCAAGCAACAATTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTAT
TTTTAGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAG
GTTGCTTTTAGGACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAG
CATCTGTCTTGATATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTT
CAAAGGTCCTGCTCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGAC
GACCCTCTTGTACCGGAGATTGCCCACATGTACAAGTCCGACAAGGCCGA
ATATGAATCCACGGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAA
GGTTGTTAGGATATTGCAGAAAAAGGAACACATTTATTGAAATATGT
back to top