CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 5.498e-65
Identity = 65/83 (78.31%), Postives = 77/83 (92.77%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNIN+NG+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDPLVPEIAH+YK+D+ KYE+TAR WT+KYA+
Sbjct:   66 PKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV 148

HSP 2 Score: 121.709 bits (304), Expect = 5.498e-65
Identity = 50/67 (74.63%), Postives = 62/67 (92.54%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            +SKRI KEL DL++DPPTSCSAGPV +D++HWQA+IMGP+DSPYAGGVF ++IHFP DYPFKPPK++
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKIS 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC1_COLGL (Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletotrichum gloeosporioides GN=UBC1 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 9.348e-65
Identity = 64/83 (77.11%), Postives = 78/83 (93.98%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICS+LTDPNPD+PLVPEIAH+YK+D+ +YE+TAR WT+KYA+
Sbjct:   65 PKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDEPLVPEIAHVYKTDRARYEATAREWTRKYAI 147

HSP 2 Score: 120.168 bits (300), Expect = 9.348e-65
Identity = 53/67 (79.10%), Postives = 58/67 (86.57%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            MA KRI KEL DL +DPP+SCSAGPV ED+FHWQATIMGP DSPY+GGVF + IHFP DYPFKPPKV
Sbjct:    1 MAFKRINKELTDLGRDPPSSCSAGPVGEDLFHWQATIMGPGDSPYSGGVFFLAIHFPTDYPFKPPKV 67
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC4_SCHPO (Ubiquitin-conjugating enzyme E2 4 OS=Schizosaccharomyces pombe GN=ubc4 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.219e-64
Identity = 65/83 (78.31%), Postives = 77/83 (92.77%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICSLLTDPNPDDPLVPEIAH+YK+D+ +YE +AR WT+KYA+
Sbjct:   65 PKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSAREWTRKYAI 147

HSP 2 Score: 120.939 bits (302), Expect = 1.219e-64
Identity = 53/67 (79.10%), Postives = 60/67 (89.55%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            MA KRI +EL DL KDPP+SCSAGPV +D+FHWQATIMGP+DSPYAGGVF ++IHFP DYPFKPPKV
Sbjct:    1 MALKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKV 67
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_XENLA (Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laevis GN=ube2d2 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.703e-64
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 2.703e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_RAT (Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norvegicus GN=Ube2d2 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.703e-64
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 2.703e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_MOUSE (Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus GN=Ube2d2 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.703e-64
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 2.703e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_HUMAN (Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens GN=UBE2D2 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.703e-64
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 2.703e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_BOVIN (Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.703e-64
Identity = 65/83 (78.31%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 2.703e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D3_PONAB (Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBC4_YEAST5.498e-6578.31Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UBC1_COLGL9.348e-6577.11Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletot... [more]
UBC4_SCHPO1.219e-6478.31Ubiquitin-conjugating enzyme E2 4 OS=Schizosacchar... [more]
UB2D2_XENLA2.703e-6478.31Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laev... [more]
UB2D2_RAT2.703e-6478.31Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norve... [more]
UB2D2_MOUSE2.703e-6478.31Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus... [more]
UB2D2_HUMAN2.703e-6478.31Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens... [more]
UB2D2_BOVIN2.703e-6478.31Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus G... [more]
UB2D3_RAT7.814e-6477.11Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve... [more]
UB2D3_PONAB7.814e-6477.11Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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