CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2C_BOVIN (Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN=UBE2C PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.151e-17
Identity = 31/66 (46.97%), Postives = 44/66 (66.67%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS 577
            AP   F T  +HPN+++ G+ICLDILK++WS    +  +LLSI SLL +PN D PL    A ++K+
Sbjct:   93 APTVKFLTPCYHPNVDTQGNICLDILKDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKN 158

HSP 2 Score: 41.9726 bits (97), Expect = 1.151e-17
Identity = 20/64 (31.25%), Postives = 33/64 (51.56%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            KR+ +EL  L        SA P ++++F W  TI G + + Y    + +++ FP  YP+  P V
Sbjct:   33 KRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTV 96
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2C_XENLA (Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis GN=ube2c PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.496e-17
Identity = 31/66 (46.97%), Postives = 46/66 (69.70%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS 577
            AP   F T  FHPN++S+G+ICLDILK++WS    +  +LLS+ SLL +PN + PL P  A ++++
Sbjct:   93 APTVKFVTPCFHPNVDSHGNICLDILKDKWSALYDVRTILLSLQSLLGEPNNESPLNPYAAELWQN 158

HSP 2 Score: 40.4318 bits (93), Expect = 1.496e-17
Identity = 21/69 (30.43%), Postives = 33/69 (47.83%), Query Frame = 3 
Query:  186 RVMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R    KR+ +EL  L        SA P ++++F W  TI G   + Y    + +++ FP  YP+  P V
Sbjct:   28 RGSVGKRLQQELMTLMMSGDKGISAFPESDNLFKWIGTIDGAVGTVYEDLRYKLSLEFPSGYPYNAPTV 96
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DICDI (Ubiquitin-conjugating enzyme E2 S OS=Dictyostelium discoideum GN=ube2s PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.937e-17
Identity = 28/82 (34.15%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN++  G IC++ LK+ W+  L +  +LL+I  LL  PN +  L  + + +   +   Y   A+ +T  +A
Sbjct:   73 PKANFITKIFHPNVSKKGEICVNTLKKDWTEDLGLKHILLTIKCLLIVPNAESSLNEDASRLLLENYDDYCKHAKLFTSIHA 154

HSP 2 Score: 50.8322 bits (120), Expect = 1.937e-17
Identity = 23/63 (36.51%), Postives = 35/63 (55.56%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            KR++KELK+L        +  P  ED+ + +A + GP+ +PY GG F   +    D+P  PPK
Sbjct:   12 KRVVKELKELNSSTLEGITLLPCEEDITNIEAVVTGPAGTPYEGGYFKARLILSSDFPRSPPK 74
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2C_MOUSE (Ubiquitin-conjugating enzyme E2 C OS=Mus musculus GN=Ube2c PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.946e-17
Identity = 31/66 (46.97%), Postives = 44/66 (66.67%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS 577
            AP   F T  +HPN+++ G+ICLDILK++WS    +  +LLSI SLL +PN D PL    A ++K+
Sbjct:   93 APTVKFLTPCYHPNVDTQGNICLDILKDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKN 158

HSP 2 Score: 41.2022 bits (95), Expect = 1.946e-17
Identity = 20/64 (31.25%), Postives = 33/64 (51.56%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            KR+ +EL  L        SA P ++++F W  TI G + + Y    + +++ FP  YP+  P V
Sbjct:   33 KRLQQELMILMTSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTV 96
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2Z_XENTR (Ubiquitin-conjugating enzyme E2 Z OS=Xenopus tropicalis GN=ube2z PE=2 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 3.246e-17
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 3 
Query:  204 RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAL 398
            RI +++  + K+PP      P   DM    A I GP D+PY GG FL     PPDYP  PP+V L
Sbjct:   62 RIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKL 126

HSP 2 Score: 46.9802 bits (110), Expect = 3.246e-17
Identity = 32/88 (36.36%), Postives = 45/88 (51.14%), Query Frame = 2 
Query:  410 FHPNINSNGSICLDIL----KEQWSPALTISKVLLSICSLLTD-PNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLGYCR 658
            F+PN   NG +CL IL       WSPA ++S VL+SI SL+T+ P  ++P   +  H    D   Y    R  T + A+   L G C+
Sbjct:  136 FNPNFYRNGKVCLSILGTWTGPAWSPAQSLSSVLISIQSLMTENPYHNEPGFEQERH--SGDSKNYNECIRHETIRVAVCEMLEGKCQ 221
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2Z_XENLA (Ubiquitin-conjugating enzyme E2 Z OS=Xenopus laevis GN=ube2z PE=2 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 3.246e-17
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 3 
Query:  204 RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAL 398
            RI +++  + K+PP      P   DM    A I GP D+PY GG FL     PPDYP  PP+V L
Sbjct:   62 RIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKL 126

HSP 2 Score: 46.9802 bits (110), Expect = 3.246e-17
Identity = 32/88 (36.36%), Postives = 45/88 (51.14%), Query Frame = 2 
Query:  410 FHPNINSNGSICLDIL----KEQWSPALTISKVLLSICSLLTD-PNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLGYCR 658
            F+PN   NG +CL IL       WSPA ++S VL+SI SL+T+ P  ++P   +  H    D   Y    R  T + A+   L G C+
Sbjct:  136 FNPNFYRNGKVCLSILGTWTGPAWSPAQSLSSVLISIQSLMTENPYHNEPGFEQERH--SGDSKNYNECIRHETIRVAVCEMLEGKCQ 221
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2R1_HUMAN (Ubiquitin-conjugating enzyme E2 R1 OS=Homo sapiens GN=CDC34 PE=1 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 3.266e-17
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            + K +L ELK LQ++P        V E D+++W+  I GP ++ Y GG F   + FP DYP+ PP
Sbjct:    9 SQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPP 73

HSP 2 Score: 51.6026 bits (122), Expect = 3.266e-17
Identity = 26/76 (34.21%), Postives = 39/76 (51.32%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDK 583
            F TK++HPNI   G +C+ IL              E+W+P   +  +LLS+ SLL +PN   P   + + MY+  K
Sbjct:   77 FLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWK 152
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2R2_RABIT (Ubiquitin-conjugating enzyme E2 R2 OS=Oryctolagus cuniculus GN=UBE2R2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 3.266e-17
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            + K ++ ELK LQ++P        V E D+++W+  I GP ++ Y GG F   I FP DYP+ PP
Sbjct:    9 SQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPP 73

HSP 2 Score: 51.9878 bits (123), Expect = 3.266e-17
Identity = 25/73 (34.25%), Postives = 39/73 (53.42%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 574
            F TK++HPNI  NG +C+ IL              E+W+P   +  +LLS+ SLL +PN   P   + + M++
Sbjct:   77 FLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFR 149
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2R2_MOUSE (Ubiquitin-conjugating enzyme E2 R2 OS=Mus musculus GN=Ube2r2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 3.266e-17
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            + K ++ ELK LQ++P        V E D+++W+  I GP ++ Y GG F   I FP DYP+ PP
Sbjct:    9 SQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPP 73

HSP 2 Score: 51.9878 bits (123), Expect = 3.266e-17
Identity = 25/73 (34.25%), Postives = 39/73 (53.42%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 574
            F TK++HPNI  NG +C+ IL              E+W+P   +  +LLS+ SLL +PN   P   + + M++
Sbjct:   77 FLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFR 149
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2R2_HUMAN (Ubiquitin-conjugating enzyme E2 R2 OS=Homo sapiens GN=UBE2R2 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 3.266e-17
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            + K ++ ELK LQ++P        V E D+++W+  I GP ++ Y GG F   I FP DYP+ PP
Sbjct:    9 SQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPP 73

HSP 2 Score: 51.9878 bits (123), Expect = 3.266e-17
Identity = 25/73 (34.25%), Postives = 39/73 (53.42%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 574
            F TK++HPNI  NG +C+ IL              E+W+P   +  +LLS+ SLL +PN   P   + + M++
Sbjct:   77 FLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFR 149
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBE2C_BOVIN1.151e-1746.97Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN... [more]
UBE2C_XENLA1.496e-1746.97Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevi... [more]
UBE2S_DICDI1.937e-1734.15Ubiquitin-conjugating enzyme E2 S OS=Dictyostelium... [more]
UBE2C_MOUSE1.946e-1746.97Ubiquitin-conjugating enzyme E2 C OS=Mus musculus ... [more]
UBE2Z_XENTR3.246e-1743.08Ubiquitin-conjugating enzyme E2 Z OS=Xenopus tropi... [more]
UBE2Z_XENLA3.246e-1743.08Ubiquitin-conjugating enzyme E2 Z OS=Xenopus laevi... [more]
UB2R1_HUMAN3.266e-1740.00Ubiquitin-conjugating enzyme E2 R1 OS=Homo sapiens... [more]
UB2R2_RABIT3.266e-1740.00Ubiquitin-conjugating enzyme E2 R2 OS=Oryctolagus ... [more]
UB2R2_MOUSE3.266e-1740.00Ubiquitin-conjugating enzyme E2 R2 OS=Mus musculus... [more]
UB2R2_HUMAN3.266e-1740.00Ubiquitin-conjugating enzyme E2 R2 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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