CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D3_MOUSE (Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D3_MACFA (Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fascicularis GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D3_HUMAN (Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens GN=UBE2D3 PE=1 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.814e-64
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 122.865 bits (307), Expect = 7.814e-64
Identity = 55/72 (76.39%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D1_MOUSE (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus GN=Ube2d1 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.259e-63
Identity = 66/83 (79.52%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YKSDK KY   AR WTQKYAM
Sbjct:   65 PKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM 147

HSP 2 Score: 118.627 bits (296), Expect = 2.259e-63
Identity = 51/68 (75.00%), Postives = 57/68 (83.82%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP DS Y GGVF +T+HFP DYPFKPPK+A
Sbjct:    1 MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIA 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.259e-63
Identity = 66/83 (79.52%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YKSDK KY   AR WTQKYAM
Sbjct:   65 PKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM 147

HSP 2 Score: 118.627 bits (296), Expect = 2.259e-63
Identity = 51/68 (75.00%), Postives = 57/68 (83.82%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP DS Y GGVF +T+HFP DYPFKPPK+A
Sbjct:    1 MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIA 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.259e-63
Identity = 66/83 (79.52%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YKSDK KY   AR WTQKYAM
Sbjct:   65 PKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM 147

HSP 2 Score: 118.627 bits (296), Expect = 2.259e-63
Identity = 51/68 (75.00%), Postives = 57/68 (83.82%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP DS Y GGVF +T+HFP DYPFKPPK+A
Sbjct:    1 MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIA 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBCD1_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=eff PE=1 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.259e-63
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WT+KYAM
Sbjct:   65 PKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELAREWTRKYAM 147

HSP 2 Score: 120.939 bits (302), Expect = 2.259e-63
Identity = 54/72 (75.00%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            MA KRI KEL+DL +DPP  CSAGPV +D+FHWQATIMGP DSPY GGVF +TIHFP DYPFKPPKVA   R
Sbjct:    1 MALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTR 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.946e-63
Identity = 64/83 (77.11%), Postives = 76/83 (91.57%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNINS+G+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDPLVPEIA +YK+DK KYE+TA+ WT+KYA+
Sbjct:   66 PKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYAV 148

HSP 2 Score: 119.398 bits (298), Expect = 2.946e-63
Identity = 51/66 (77.27%), Postives = 59/66 (89.39%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +SKRI KEL DL +DPP SCSAGPV +D++HWQA+IMGPSDSPYAGGVF ++IHFP DYPFKPPKV
Sbjct:    3 SSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKV 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 3.841e-63
Identity = 65/83 (78.31%), Postives = 75/83 (90.36%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK    TK++HPNINSNG+ICLDILK+QWSPALTISKVLLSICSLLTD NPDDPLVPEIAH+YK D+ KYE+TA+ WT+KYA+
Sbjct:   65 PKIALTTKIYHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHIYKQDRKKYEATAKEWTKKYAV 147

HSP 2 Score: 117.087 bits (292), Expect = 3.841e-63
Identity = 50/69 (72.46%), Postives = 60/69 (86.96%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAL 398
            M+ KRI KEL DL +DPP+SCSAGPV +D++HWQA+IMGP DSPYAGGVF ++IHFP DYP KPPK+AL
Sbjct:    1 MSLKRINKELSDLGRDPPSSCSAGPVGDDLYHWQASIMGPPDSPYAGGVFFLSIHFPTDYPLKPPKIAL 69
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UB2D3_MOUSE7.814e-6477.11Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus... [more]
UB2D3_MACFA7.814e-6477.11Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fasci... [more]
UB2D3_HUMAN7.814e-6477.11Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens... [more]
UB2D2_PIG7.814e-6477.11Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G... [more]
UB2D1_MOUSE2.259e-6379.52Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus... [more]
UB2D1_HUMAN2.259e-6379.52Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens... [more]
UB2D1_BOVIN2.259e-6379.52Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]
UBCD1_DROME2.259e-6377.11Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi... [more]
UBC5_YEAST2.946e-6377.11Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
UBC4_CANAL3.841e-6378.31Ubiquitin-conjugating enzyme E2 4 OS=Candida albic... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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