CK937528 | Citrus Genome Database

Overview


Name	CK937528
Unique Name	CK937528
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	712

Libraries


Library Name	Type
Developing fruit albedo at 80 DAFB in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.723e-44
Identity = 53/82 (64.63%), Postives = 65/82 (79.27%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++ +++  AR WT++YA
Sbjct:  125 PKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 85.5001 bits (210), Expect = 1.723e-44
Identity = 37/71 (52.11%), Postives = 50/71 (70.42%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV    R
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTR 132

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.723e-44
Identity = 53/82 (64.63%), Postives = 65/82 (79.27%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++ +++  AR WT++YA
Sbjct:  125 PKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 85.5001 bits (210), Expect = 1.723e-44
Identity = 37/71 (52.11%), Postives = 50/71 (70.42%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV    R
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTR 132

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.723e-44
Identity = 53/82 (64.63%), Postives = 65/82 (79.27%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++ +++  AR WT++YA
Sbjct:  125 PKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 85.5001 bits (210), Expect = 1.723e-44
Identity = 37/71 (52.11%), Postives = 50/71 (70.42%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV    R
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTR 132

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.918e-37
Identity = 38/71 (53.52%), Postives = 51/71 (71.83%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGPR 407
            + KRI +E+ +L  DPP  CSAGP  ++++HW ATI+GPS +PY GG+F + I FP DYPFKPPK+    R
Sbjct:   37 SGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTR 107

HSP 2 Score: 82.8037 bits (203), Expect = 3.918e-37
Identity = 34/82 (41.46%), Postives = 58/82 (70.73%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F+T+++H N+++ G + ++IL++ WSPALTI+KVL +I S+   P P  P +P IA +Y +D+ K++  A+ WT ++A
Sbjct:  100 PKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFA 181

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC31_ARATH (Probable ubiquitin carrier protein E2 31 OS=Arabidopsis thaliana GN=UBC31 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.415e-33
Identity = 44/82 (53.66%), Postives = 64/82 (78.05%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F+T ++HPNIN  GSIC++ILK++W+PAL + KVLLSI  LL  PNPDDPLVPEI  ++K+++ +++  AR +T ++A
Sbjct:   71 PKFTFKTPIYHPNINDEGSICMNILKDKWTPALMVEKVLLSILLLLEKPNPDDPLVPEIGQLFKNNRFQFDQRAREFTARHA 152

HSP 2 Score: 57.3806 bits (137), Expect = 2.415e-33
Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3

Query:  255 SAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            S G  + +++ W A I GP  +PY GG+F ++I FP DYPFKPPK
Sbjct:   28 SIGQNSNNIYEWTAVIRGPDGTPYEGGMFNLSIKFPTDYPFKPPK 72

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.146e-33
Identity = 49/85 (57.65%), Postives = 62/85 (72.94%), Query Frame = 2

Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            APK  F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KS++ +   TA+ WT+ YA G
Sbjct:   68 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAKEWTRLYASG 152

HSP 2 Score: 60.4622 bits (145), Expect = 3.146e-33
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P    SA P  E+M ++   I+GP+ SPY GGVF + +  P +YP   PKV
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKV 71

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 6.950e-33
Identity = 49/85 (57.65%), Postives = 62/85 (72.94%), Query Frame = 2

Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            APK  F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KS++ +   TA+ WT+ YA G
Sbjct:   68 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASG 152

HSP 2 Score: 59.3066 bits (142), Expect = 6.950e-33
Identity = 26/64 (40.62%), Postives = 39/64 (60.94%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P    SA P  ++M ++   I+GP+ SPY GGVF + +  P +YP   PKV
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKV 71

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBCB_DICDI (Ubiquitin conjugating enzyme E2 B OS=Dictyostelium discoideum GN=ubcB PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.537e-32
Identity = 41/79 (51.90%), Postives = 56/79 (70.89%), Query Frame = 2

Query:  395 FRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            F TK++HPNI  S+G+IC ++    WSP L I  VL +I S+LTDPNPD+PL  EIA  +K+D+  +  TA+ WT+KYA
Sbjct:   70 FTTKIYHPNIKTSDGAICAEVFST-WSPQLKILDVLTTIRSILTDPNPDNPLETEIAQQFKTDRNAFNKTAKEWTKKYA 147

HSP 2 Score: 72.4034 bits (176), Expect = 1.537e-32
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            A KR+ KE+ D+ K PP+ CSA  V +++  W+AT+ GP  SP+  GVF + I  P DYPFKPP
Sbjct:    3 AHKRLQKEITDMLKTPPSWCSAHLVDDNLQKWKATVQGPEGSPFEKGVFSMDIDIPADYPFKPP 66

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.271e-31
Identity = 46/84 (54.76%), Postives = 63/84 (75.00%), Query Frame = 2

Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPNI+  G ICLD+LK++WSPAL I  +LLSI +LL+ PNPDDPL  ++A ++K ++ +    AR WTQKYA+
Sbjct:   66 APKVRFITKIYHPNIDRLGRICLDVLKDKWSPALQIRTILLSIQALLSAPNPDDPLANDVAELWKVNEAEAIRNAREWTQKYAV 149

HSP 2 Score: 55.0694 bits (131), Expect = 1.271e-31
Identity = 24/64 (37.50%), Postives = 38/64 (59.38%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L ++P    +A P   +  ++   + GP+DSP+ GGVF + +  P DYP   PKV
Sbjct:    6 RRIIKETQRLMQEPVPGINAIPDENNARYFHVIVTGPNDSPFEGGVFKLELFLPEDYPMSAPKV 69

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.155e-31
Identity = 47/84 (55.95%), Postives = 65/84 (77.38%), Query Frame = 2

Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++ +G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKSGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 2.155e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69

The following BLAST results are available for this feature:

BLAST of CK937528 vs. ExPASy Swiss-Prot

BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246


Match Name	E-value	Identity	Description
UB2E3_MOUSE	1.723e-44	64.63	Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus...	[more]
UB2E3_HUMAN	1.723e-44	64.63	Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens...	[more]
UB2E3_BOVIN	1.723e-44	64.63	Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G...	[more]
COP10_ARATH	3.918e-37	53.52	Constitutive photomorphogenesis protein 10 OS=Arab...	[more]
UBC31_ARATH	2.415e-33	53.66	Probable ubiquitin carrier protein E2 31 OS=Arabid...	[more]
UBC36_ARATH	3.146e-33	57.65	Ubiquitin carrier protein E2 36 OS=Arabidopsis tha...	[more]
UBC35_ARATH	6.950e-33	57.65	Ubiquitin carrier protein E2 35 OS=Arabidopsis tha...	[more]
UBCB_DICDI	1.537e-32	51.90	Ubiquitin conjugating enzyme E2 B OS=Dictyostelium...	[more]
UBE2N_DROME	1.271e-31	54.76	Ubiquitin-conjugating enzyme E2 N OS=Drosophila me...	[more]
UBE2N_MACFA	2.155e-31	55.95	Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT