CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.806e-31
Identity = 48/84 (57.14%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +KS++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 2.806e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_DICDI (Probable ubiquitin-conjugating enzyme E2 N OS=Dictyostelium discoideum GN=ube2n PE=3 SV=2)

HSP 1 Score: 97.4413 bits (241), Expect = 3.654e-31
Identity = 46/83 (55.42%), Postives = 62/83 (74.70%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            APK  F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +  + AR WT+ YA
Sbjct:   66 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEHWKTNEKEAMARAREWTRLYA 148

HSP 2 Score: 56.9954 bits (136), Expect = 3.654e-31
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            KRI+KE + L  +P    SA P  ++  +++  I GP DSP+ GGVF + +    DYP   PKV
Sbjct:    6 KRIIKETQRLMTEPAPGISATPATDNFRYFKVAISGPLDSPFEGGVFNLELFLTDDYPMSAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC3_ARATH (Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis thaliana GN=UBC3 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 4.760e-31
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A KR++ + K LQKDPP   S  P   ++ HW A I GP D+P+ GG F +T+HF  DYP KPP V
Sbjct:    5 AKKRLMWDFKRLQKDPPVGISGAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIV 70

HSP 2 Score: 77.0258 bits (188), Expect = 4.760e-31
Identity = 35/66 (53.03%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKY 592
            F +++FHPNI ++GSICLDIL+ QWSP   ++ VL SI SLL DPNPD P   E A ++  +K +Y
Sbjct:   72 FVSRMFHPNIYADGSICLDILQNQWSPIYDVAAVLTSIQSLLCDPNPDSPANAEAARLFSENKREY 137
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.197e-31
Identity = 47/84 (55.95%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 6.197e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.197e-31
Identity = 47/84 (55.95%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 6.197e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.197e-31
Identity = 47/84 (55.95%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 6.197e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2N_BOVIN (Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN=UBE2N PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.197e-31
Identity = 47/84 (55.95%), Postives = 64/84 (76.19%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++ +   TAR+WT+ YAM
Sbjct:   66 APKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 50.8322 bits (120), Expect = 6.197e-31
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +RI+KE + L  +P     A P   +  ++   I GP DSP+ GG F + +  P +YP   PKV
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC13_YEAST (Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyces cerevisiae GN=UBC13 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.934e-30
Identity = 43/84 (51.19%), Postives = 58/84 (69.05%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            +APK  F TK++HPNI+  G ICLD+LK  WSPAL I  VLLSI +LL  PNP+DPL  ++A  +  ++   ++ AR WT+ YA
Sbjct:   65 EAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKAREWTKLYA 148

HSP 2 Score: 60.4622 bits (145), Expect = 3.934e-30
Identity = 27/64 (42.19%), Postives = 40/64 (62.50%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            KRI+KE + L  DP    +A P  +++ ++Q TI GP  SPY  G+F + ++ P DYP + PKV
Sbjct:    6 KRIIKETEKLVSDPVPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKV 69
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC1_SCHPO (Ubiquitin-conjugating enzyme E2 1 OS=Schizosaccharomyces pombe GN=ubc1 PE=2 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 8.633e-30
Identity = 39/83 (46.99%), Postives = 55/83 (66.27%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSN-GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK++HPN++S  G+ICLDILK+QWSP  T+   L+S+ SLL  P P +P   ++A +Y  +  ++  TAR WT  YA
Sbjct:   68 PKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTAREWTSSYA 150

HSP 2 Score: 64.6994 bits (156), Expect = 8.633e-30
Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            S+RI KEL D+Q+D         + +D+ H +    GP  +PY GG F+V I  P DYPF+PPK+
Sbjct:    6 SRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKM 70
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_WHEAT (Ubiquitin-conjugating enzyme E2 2 OS=Triticum aestivum GN=UBC2 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.686e-30
Identity = 39/79 (49.37%), Postives = 52/79 (65.82%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKY-----ESTARSWT 616
            F +++FHPNI ++GSICLDIL+ QWSP   ++ +L SI SLL DPNP+ P   E A MY  +K +Y     E   +SWT
Sbjct:   72 FVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMYSENKREYNRKVREVVEQSWT 150

HSP 2 Score: 69.707 bits (169), Expect = 8.686e-30
Identity = 30/66 (45.45%), Postives = 41/66 (62.12%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A KR++++ K LQ+DPP   S  P   ++  W A I GP D+P+ GG F +T+ F  DYP KPP V
Sbjct:    5 ARKRLMRDFKRLQQDPPAGISGAPHDNNITLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTV 70
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBE2N_RAT2.806e-3157.14Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBE2N_DICDI3.654e-3155.42Probable ubiquitin-conjugating enzyme E2 N OS=Dict... [more]
UBC3_ARATH4.760e-3150.00Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis t... [more]
UBE2N_PONAB6.197e-3155.95Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ... [more]
UBE2N_MOUSE6.197e-3155.95Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ... [more]
UBE2N_HUMAN6.197e-3155.95Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ... [more]
UBE2N_BOVIN6.197e-3155.95Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN... [more]
UBC13_YEAST3.934e-3051.19Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyce... [more]
UBC1_SCHPO8.633e-3046.99Ubiquitin-conjugating enzyme E2 1 OS=Schizosacchar... [more]
UBC2_WHEAT8.686e-3049.37Ubiquitin-conjugating enzyme E2 2 OS=Triticum aest... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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