CK937528 | Citrus Genome Database

Overview


Name	CK937528
Unique Name	CK937528
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	712

Libraries


Library Name	Type
Developing fruit albedo at 80 DAFB in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_YARLI (Ubiquitin-conjugating enzyme E2 2 OS=Yarrowia lipolytica GN=UBC2 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.217e-27
Identity = 33/79 (41.77%), Postives = 49/79 (62.03%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR-----SWT 616
            F +++FHPN+ S+G +CLDIL+ +WSP   ++ +L S+ SLL DPN   P   E + +YK  + +YE   R     SWT
Sbjct:   72 FVSQMFHPNVYSSGELCLDILQNRWSPTYDVAAILTSVQSLLNDPNTSSPANVEASMLYKDHRQQYEKRVRDTVEASWT 150

HSP 2 Score: 68.9366 bits (167), Expect = 2.217e-27
Identity = 27/66 (40.91%), Postives = 44/66 (66.67%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K +Q+DPP   SA PVA+++  W A I+GP+++P+  G F + + F   YP KPP V
Sbjct:    5 ARRRLMRDFKRMQQDPPQGVSASPVADNVLTWNAVIIGPAETPFEDGTFRMVLQFDEQYPNKPPAV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.885e-27
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   72 FLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 63.929 bits (154), Expect = 2.885e-27
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K LQ+DPP   S  P   ++  W A I GP  +P+  G F + I F  +YP KPP V
Sbjct:    5 ARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.885e-27
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   72 FLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 63.929 bits (154), Expect = 2.885e-27
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K LQ+DPP   S  P   ++  W A I GP  +P+  G F + I F  +YP KPP V
Sbjct:    5 ARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.885e-27
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   72 FLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 63.929 bits (154), Expect = 2.885e-27
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K LQ+DPP   S  P   ++  W A I GP  +P+  G F + I F  +YP KPP V
Sbjct:    5 ARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.885e-27
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   72 FLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 63.929 bits (154), Expect = 2.885e-27
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K LQ+DPP   S  P   ++  W A I GP  +P+  G F + I F  +YP KPP V
Sbjct:    5 ARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.885e-27
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = 2

Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   72 FLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 63.929 bits (154), Expect = 2.885e-27
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K LQ+DPP   S  P   ++  W A I GP  +P+  G F + I F  +YP KPP V
Sbjct:    5 ARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2T_XENLA (Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevis GN=ube2t PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.738e-27
Identity = 43/89 (48.31%), Postives = 61/89 (68.54%), Query Frame = 2

Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            + PK  F T ++HPNI+S G ICLDILK      W PAL IS VL SI  L+++PNPDDPL+ +I+  +K ++  + S A+ WT+K+A+
Sbjct:   64 EPPKIRFLTPIYHPNIDSAGRICLDILKLPPKGAWRPALNISTVLTSIQLLMSEPNPDDPLMADISSEFKYNRAVFFSNAKKWTEKHAL 152

HSP 2 Score: 52.7582 bits (125), Expect = 3.738e-27
Identity = 26/63 (41.27%), Postives = 37/63 (58.73%), Query Frame = 3

Query:  204 RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R+ +EL+ L K+PP          +M   +A I+G S SPY GG+F + I  P  YPF+PPK+
Sbjct:    6 RLKRELQLLNKEPPPGVICWQNESNMDDLRAQIIGGSGSPYEGGIFNLEIIVPERYPFEPPKI 68

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_NECHA (Ubiquitin-conjugating enzyme E2 2 OS=Nectria haematococca GN=UBC2 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.757e-27
Identity = 32/80 (40.00%), Postives = 50/80 (62.50%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQK 622
            P+  F +++FHPN+ + G +CLDIL+ +WSP   ++ VL SI SLL DPN   P   E +++YK ++ +Y    R   +K
Sbjct:   68 PQVKFISEMFHPNVYATGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRKEYTKRVRETVEK 147

HSP 2 Score: 68.5514 bits (166), Expect = 3.757e-27
Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K +Q DPP   SA PV +++  W A I+GP+D+P+  G F + + F   YP KPP+V
Sbjct:    5 ARRRLMRDFKRMQTDPPAGVSASPVPDNVMTWNAVIIGPADTPFEDGTFRLVMQFEEQYPNKPPQV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_CANAL (Ubiquitin-conjugating enzyme E2 2 OS=Candida albicans GN=UBC2 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.872e-27
Identity = 33/82 (40.24%), Postives = 50/82 (60.98%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKY-----ESTARSW 613
            P+  F +++FHPN+ ++G +CLDIL+ +WSP   +S +L S+ SLL DPN   P   E A++YK  +  Y     E+   SW
Sbjct:   68 PQVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSVQSLLNDPNISSPANVEAANLYKDHRSLYVKRVRETVENSW 149

HSP 2 Score: 69.707 bits (169), Expect = 4.872e-27
Identity = 27/66 (40.91%), Postives = 45/66 (68.18%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K +Q+DPP+  SA P+ +++  W A I+GPSD+P+  G F + + F   YP KPP+V
Sbjct:    5 ARRRLMRDFKRMQQDPPSGVSASPLPDNVMKWNAVIIGPSDTPFEDGTFRLLLSFDEQYPNKPPQV 70

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_NEUCR (Ubiquitin-conjugating enzyme E2 2 OS=Neurospora crassa GN=mus-8 PE=3 SV=3)

HSP 1 Score: 71.2478 bits (173), Expect = 6.366e-27
Identity = 28/66 (42.42%), Postives = 43/66 (65.15%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K +Q DPP   SA PV +++  W A I+GP+D+P+  G F + +HF   YP KPP V
Sbjct:    5 ARRRLMRDFKRMQTDPPAGVSASPVPDNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPSV 70

HSP 2 Score: 68.9366 bits (167), Expect = 6.366e-27
Identity = 31/80 (38.75%), Postives = 48/80 (60.00%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQK 622
            P   F +++FHPN+ + G +CLDIL+ +WSP   ++ VL SI SLL DPN       E +++YK ++ +Y    R   +K
Sbjct:   68 PSVKFISEMFHPNVYATGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSRANVEASNLYKDNRKEYHKRVRETVEK 147

The following BLAST results are available for this feature:

BLAST of CK937528 vs. ExPASy Swiss-Prot

BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246


Match Name	E-value	Identity	Description
UBC2_YARLI	2.217e-27	41.77	Ubiquitin-conjugating enzyme E2 2 OS=Yarrowia lipo...	[more]
UBE2B_RAT	2.885e-27	49.25	Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg...	[more]
UBE2B_RABIT	2.885e-27	49.25	Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c...	[more]
UBE2B_MOUSE	2.885e-27	49.25	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]
UBE2B_HUMAN	2.885e-27	49.25	Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ...	[more]
UBE2B_BOVIN	2.885e-27	49.25	Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN...	[more]
UBE2T_XENLA	3.738e-27	48.31	Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevi...	[more]
UBC2_NECHA	3.757e-27	40.00	Ubiquitin-conjugating enzyme E2 2 OS=Nectria haema...	[more]
UBC2_CANAL	4.872e-27	40.24	Ubiquitin-conjugating enzyme E2 2 OS=Candida albic...	[more]
UBC2_NEUCR	6.366e-27	42.42	Ubiquitin-conjugating enzyme E2 2 OS=Neurospora cr...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT