CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2K_MOUSE (Ubiquitin-conjugating enzyme E2 K OS=Mus musculus GN=Ube2k PE=2 SV=3)

HSP 1 Score: 83.5741 bits (205), Expect = 7.281e-25
Identity = 41/83 (49.40%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   71 PKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 49.6766 bits (117), Expect = 7.281e-25
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTS---CSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +A +RI +E K++ K   TS        V E+    +  I GP D+PY GG + + I  P  YPF PPKV
Sbjct:    4 IAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKV 73
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2K_HUMAN (Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens GN=UBE2K PE=1 SV=3)

HSP 1 Score: 83.5741 bits (205), Expect = 7.281e-25
Identity = 41/83 (49.40%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   71 PKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 49.6766 bits (117), Expect = 7.281e-25
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTS---CSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +A +RI +E K++ K   TS        V E+    +  I GP D+PY GG + + I  P  YPF PPKV
Sbjct:    4 IAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKV 73
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2K_BOVIN (Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN=UBE2K PE=1 SV=3)

HSP 1 Score: 83.5741 bits (205), Expect = 7.281e-25
Identity = 41/83 (49.40%), Postives = 56/83 (67.47%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   71 PKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 49.6766 bits (117), Expect = 7.281e-25
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 3 
Query:  192 MASKRILKELKDLQKDPPTS---CSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +A +RI +E K++ K   TS        V E+    +  I GP D+PY GG + + I  P  YPF PPKV
Sbjct:    4 IAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKV 73
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UE2NL_HUMAN (Putative ubiquitin-conjugating enzyme E2 N-like OS=Homo sapiens GN=UBE2NL PE=5 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.240e-24
Identity = 44/84 (52.38%), Postives = 60/84 (71.43%), Query Frame = 2 
Query:  380 APKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            APK  F TK++HPN++    I LDILK++WSPAL I  VLLSI +LL  PNPDDPL  ++   +K+++ +   TAR+WT+ YAM
Sbjct:   67 APKVRFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQAIETARAWTRLYAM 150

HSP 2 Score: 38.5058 bits (88), Expect = 1.240e-24
Identity = 22/64 (34.38%), Postives = 31/64 (48.44%), Query Frame = 3 
Query:  204 RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPS-DSPYAGGVFLVTIHFPPDYPFKPPKV 392
            RI+KE + L  +P     A P   +  ++   I G S DSP+ GG F   +    +YP   PKV
Sbjct:    7 RIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKV 70
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_XENTR (Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropicalis GN=ube2s PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.087e-24
Identity = 39/89 (43.82%), Postives = 54/89 (60.67%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLG 649
            PKG F TK+FHPN+++NG IC+++LK+ W   L I  VLL+I  LL  PNP+  L  E   +   +  +Y S AR  T+ +A G  L G
Sbjct:   75 PKGYFLTKIFHPNVSTNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTEIHAQGSSLRG 163

HSP 2 Score: 52.7582 bits (125), Expect = 2.087e-24
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++ KE+  L  DPP      P  ED+   Q  I GP  +PYAGG+F + +    D+P  PPK
Sbjct:   14 RQVYKEVSTLTSDPPEGIKIIPNEEDITDVQVNIEGPEGTPYAGGIFRMKLILGKDFPAAPPK 76
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2SB_XENLA (Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus laevis GN=ube2s-B PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.087e-24
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLG 649
            PKG F TK+FHPN+++NG IC+++LK+ W   L I  VLL+I  LL  PNP+  L  E   +   +  +Y S AR  T  +A G  L G
Sbjct:   75 PKGYFLTKIFHPNVSNNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTDIHAQGTSLRG 163

HSP 2 Score: 53.5286 bits (127), Expect = 2.087e-24
Identity = 25/63 (39.68%), Postives = 34/63 (53.97%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +R+ KE+  L  DPP      P  ED+   Q  I GP  +PYAGG+F + +    D+P  PPK
Sbjct:   14 RRVYKEVSTLTSDPPEGIKIIPNEEDITDVQVNIEGPEGTPYAGGMFRMKLILGKDFPAAPPK 76
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2T_BOVIN (Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN=UBE2T PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.540e-24
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            + P+  F T ++HPNI+S G ICLD+LK      W P+L I+ +L  I  L+ +PNPDDPL+ +I+  +K +K  +   AR WT+K+A
Sbjct:   64 EPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATLLTCIQQLMAEPNPDDPLMADISSEFKYNKPVFFKNARQWTEKHA 151

HSP 2 Score: 50.447 bits (119), Expect = 3.540e-24
Identity = 22/65 (33.85%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            + R+ +EL  L  +PP   +     + M   +A I+G +++PY  GVF + +H P  YPF+PP++
Sbjct:    4 TSRLKRELSLLAAEPPPGITCWQDGDRMEDLRAQILGGANTPYEKGVFKLEVHIPERYPFEPPQI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2T_MOUSE (Ubiquitin-conjugating enzyme E2 T OS=Mus musculus GN=Ube2t PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 5.990e-24
Identity = 39/88 (44.32%), Postives = 59/88 (67.05%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            + P+  F T ++HPNI+S+G ICLDILK      W P+L I+ VL SI  L+ +PNPDDPL+ +I+  +K +K+ +   A+ WT+ +A
Sbjct:   64 EPPQVRFLTPIYHPNIDSSGRICLDILKLPPKGAWRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAKQWTEAHA 151

HSP 2 Score: 46.9802 bits (110), Expect = 5.990e-24
Identity = 22/65 (33.85%), Postives = 37/65 (56.92%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            + R+ KEL  L  +PP   +     + +   +A I+G +++PY  GVF + +  P  YPF+PP+V
Sbjct:    4 ASRLKKELHMLAIEPPPGITCWQEKDQVADLRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQV 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2T_HUMAN (Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens GN=UBE2T PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 5.994e-24
Identity = 40/88 (45.45%), Postives = 59/88 (67.05%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            + P+  F T ++HPNI+S G ICLD+LK      W P+L I+ VL SI  L+++PNPDDPL+ +I+  +K +K  +   AR WT+K+A
Sbjct:   64 EPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHA 151

HSP 2 Score: 46.595 bits (109), Expect = 5.994e-24
Identity = 21/65 (32.31%), Postives = 37/65 (56.92%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            + R+ +EL  L  +PP   +     + M   +A I+G +++PY  GVF + +  P  YPF+PP++
Sbjct:    4 ASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_RANCA (Ubiquitin-conjugating enzyme E2 S OS=Rana catesbeiana GN=ube2s PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.791e-24
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLG 649
            PKG F TK+FHPN++SNG IC+++LK+ W   L I  VLL+I  LL  PNP+  L  E   +   +  +Y S AR  T+ +A    L G
Sbjct:   75 PKGYFLTKIFHPNVSSNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTEIHAHSSSLRG 163

HSP 2 Score: 52.7582 bits (125), Expect = 7.791e-24
Identity = 24/63 (38.10%), Postives = 35/63 (55.56%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++ KE+  L  DPP      P  ED+   Q +I GP  +PYAGG+F + +    D+P  PPK
Sbjct:   14 RQVYKEVSTLTPDPPEGIKIIPNEEDITDVQVSIEGPEGTPYAGGIFRMKLILGKDFPAAPPK 76
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBE2K_MOUSE7.281e-2549.40Ubiquitin-conjugating enzyme E2 K OS=Mus musculus ... [more]
UBE2K_HUMAN7.281e-2549.40Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens ... [more]
UBE2K_BOVIN7.281e-2549.40Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN... [more]
UE2NL_HUMAN1.240e-2452.38Putative ubiquitin-conjugating enzyme E2 N-like OS... [more]
UBE2S_XENTR2.087e-2443.82Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropi... [more]
UB2SB_XENLA2.087e-2443.82Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus lae... [more]
UBE2T_BOVIN3.540e-2443.18Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN... [more]
UBE2T_MOUSE5.990e-2444.32Ubiquitin-conjugating enzyme E2 T OS=Mus musculus ... [more]
UBE2T_HUMAN5.994e-2445.45Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens ... [more]
UBE2S_RANCA7.791e-2443.82Ubiquitin-conjugating enzyme E2 S OS=Rana catesbei... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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