CK936872 | Citrus Genome Database

Overview


Name	CK936872
Unique Name	CK936872
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	735

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEAM (NAD-dependent malic enzyme OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 7.608e-28
Identity = 58/143 (40.56%), Postives = 93/143 (65.03%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFTE+ER    L GLLP A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N     +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTEEERIFFNLEGLLPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNIAEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRHKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 7.608e-28
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBCH (NAD-dependent malic enzyme OS=Vibrio cholerae GN=sfcA PE=3 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 7.608e-28
Identity = 62/150 (41.33%), Postives = 97/150 (64.67%), Query Frame = 2

Query:  197 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            P  IS A G  LL  P  NKG AF+ +ER +  L GLLP A  + Q Q  +     R +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS   + +I ++L N    +++VIVVTDGERI
Sbjct:    7 PLYISYA-GPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 7.608e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBC3 (NAD-dependent malic enzyme OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sfcA PE=3 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 7.608e-28
Identity = 62/150 (41.33%), Postives = 97/150 (64.67%), Query Frame = 2

Query:  197 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            P  IS A G  LL  P  NKG AF+ +ER +  L GLLP A  + Q Q  +     R +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS   + +I ++L N    +++VIVVTDGERI
Sbjct:    7 PLYISYA-GPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 7.608e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SODGM (NAD-dependent malic enzyme OS=Sodalis glossinidius (strain morsitans) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 9.903e-28
Identity = 57/143 (39.86%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ERD   L+GLLP  V + + Q ++     + +   + +++ +  +++ NE LFY+LL  ++ E+LP++YTPTVG+AC+++  I+RR +G++IS     KI ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFSLEERDNFNLQGLLPDTVETIEEQAERAWRQYQDFRTNIDRHIYLRNIQDTNETLFYRLLAAHLAEMLPIIYTPTVGDACERFSDIYRRARGVFISYNNCDKIEDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 9.903e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PROMH (NAD-dependent malic enzyme OS=Proteus mirabilis (strain HI4320) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 9.903e-28
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+E+ER    L GLLP AV + + Q ++       ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFSEEERSTFNLHGLLPEAVETIEEQVERAYRQYLDFKNDNDKHIYLRNIQDTNETLFYRLLESHLTEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRANIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 9.903e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PSYIN (NAD-dependent malic enzyme OS=Psychromonas ingrahamii (strain 37) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 9.904e-28
Identity = 56/143 (39.16%), Postives = 99/143 (69.23%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+++ER+   L GLLP  + + + Q  +    +  ++  L K++ +  +++ NE LF+ L+ +++EE++P++YTPTVG AC+++  I+RR +GL++S  E+ KI ++L+N  +++++VIVVTDGERI
Sbjct:   12 AGPALLESPLLNKGSAFSQEERNNFNLTGLLPHNIETIESQSTRAYQQLSSFKSDLDKHIYLRNIQDTNETLFHHLIENHLEEVMPLIYTPTVGLACEQFSKIYRRKRGLFVSYPERHKIDDMLQNATKQNVKVIVVTDGERI 154

HSP 2 Score: 22.3274 bits (46), Expect = 9.904e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  169 IGKLALYTACGGISP 183

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO11_PHOPR (NAD-dependent malic enzyme 1 OS=Photobacterium profundum GN=sfcA1 PE=3 SV=2)

HSP 1 Score: 120.168 bits (300), Expect = 1.289e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP A+ S + Q ++     ++++  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS   + +I ++L N   ++++VIVVTDGERI
Sbjct:   13 AGPALLETPLLNKGSAFSIEERMFFNLEGLLPEAIESIEEQTERAYQQYQKFDKDMDKHIYLRNIQDTNETLFYRLVENHITEMMPIIYTPTVGAACEDFSNIYRRGRGLFISYPNRDRIEDMLNNASRQNVKVIVVTDGERI 155

HSP 2 Score: 22.3274 bits (46), Expect = 1.289e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLALYTACGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO12_VIBVY (NAD-dependent malic enzyme 2 OS=Vibrio vulnificus (strain YJ016) GN=sfcA2 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.289e-27
Identity = 55/143 (38.46%), Postives = 93/143 (65.03%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT +ER    L GLLP  + +   Q  +       +   ++K++ +  +++ NE L+++L+  ++EE++P++YTPTVGEACQK+  I+RR +GL++S +++ ++  +L N P   ++VIV+TDGERI
Sbjct:   10 AGNTLLNTPLLNKGSAFTLEERKNFNLIGLLPANIETIDEQVSRAYEQFSLFNSAMEKHIYLRNIQDTNETLYFRLINQHIEEMMPIIYTPTVGEACQKFSQIYRRNRGLFLSFEDQDELEAILNNAPNTHVKVIVITDGERI 152

HSP 2 Score: 22.3274 bits (46), Expect = 1.289e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  167 IGKLALYTACGGISP 181

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SALG2 (NAD-dependent malic enzyme OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.678e-27
Identity = 58/143 (40.56%), Postives = 96/143 (67.13%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 1.678e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBPA (NAD-dependent malic enzyme OS=Vibrio parahaemolyticus GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.678e-27
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  L+  P  NKG AF+ +ER +  L GLLP    + Q Q ++     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS   + +I ++L N    +++VIVVTDGERI
Sbjct:   13 AGPALMATPLLNKGSAFSAEERSSFNLEGLLPETTETIQEQVERAYQQYKSFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYPNRDRIDDLLNNAANHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 1.678e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184

The following BLAST results are available for this feature:

BLAST of CK936872 vs. ExPASy Swiss-Prot

BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_SHEAM	7.608e-28	40.56	NAD-dependent malic enzyme OS=Shewanella amazonens...	[more]
MAO1_VIBCH	7.608e-28	41.33	NAD-dependent malic enzyme OS=Vibrio cholerae GN=s...	[more]
MAO1_VIBC3	7.608e-28	41.33	NAD-dependent malic enzyme OS=Vibrio cholerae sero...	[more]
MAO1_SODGM	9.903e-28	39.86	NAD-dependent malic enzyme OS=Sodalis glossinidius...	[more]
MAO1_PROMH	9.903e-28	41.26	NAD-dependent malic enzyme OS=Proteus mirabilis (s...	[more]
MAO1_PSYIN	9.904e-28	39.16	NAD-dependent malic enzyme OS=Psychromonas ingraha...	[more]
MAO11_PHOPR	1.289e-27	39.16	NAD-dependent malic enzyme 1 OS=Photobacterium pro...	[more]
MAO12_VIBVY	1.289e-27	38.46	NAD-dependent malic enzyme 2 OS=Vibrio vulnificus ...	[more]
MAO1_SALG2	1.678e-27	40.56	NAD-dependent malic enzyme OS=Salmonella gallinaru...	[more]
MAO1_VIBPA	1.678e-27	38.46	NAD-dependent malic enzyme OS=Vibrio parahaemolyti...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC