CK936838

Overview
NameCK936838
Unique NameCK936838
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length720
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: SAR1_CANGA (Small COPII coat GTPase SAR1 OS=Candida glabrata GN=SAR1 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.021e-19
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 3 
Query:  111 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDEL 386
            K  ++L +GLD AGKTT+L+ LK   + T  PT     E +   NI FT +D+GG  + R LW+ YF    G++F+VDS D DR  EAR EL
Sbjct:   21 KHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHVQARRLWKDYFPEVNGIVFLVDSADPDRFDEARVEL 112

HSP 2 Score: 33.113 bits (74), Expect = 5.021e-19
Identity = 22/75 (29.33%), Postives = 31/75 (41.33%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR-------QRHWYIQSTCATSGEGLYEGLDWLSNNI 595
            + N  EL+D   ++  NK D  NA++ AE+   LGL +         QR   +         G  E   WLS  I
Sbjct:  115 LFNITELKDVPFVILGNKIDAANAVSEAELRSALGLLNTTGSQRIEGQRPVEVFMCSVVMRNGYLEAFQWLSQYI 189
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: AR13B_DANRE (ADP-ribosylation factor-like protein 13B OS=Danio rerio GN=arl13b PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.408e-18
Identity = 32/92 (34.78%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  111 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDEL 386
            +++ ++MVGLD AGKT  +  ++    +   PT+GF+   ++      T++D+GG  +IR +W++Y+  + G++FVVDS+D  R+ E RD +
Sbjct:   20 RKVTLVMVGLDNAGKTATVRGIQGESPLDVAPTVGFSKVDLKQGKFEVTIFDLGGGKRIRGIWKNYYSESYGVVFVVDSSDVQRIQETRDTM 111

HSP 2 Score: 38.1206 bits (87), Expect = 1.408e-18
Identity = 28/80 (35.00%), Postives = 37/80 (46.25%), Query Frame = 2 
Query:  386 APMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRH---WYIQSTCATSGEG------LYEGLDWLSNNIA 598
            A +L    +    +LV ANKQD   AM  A+I + L L  L   +     I+   A  G G      +  GL+WL NNIA
Sbjct:  112 AEVLRHPRIAGKPVLVLANKQDQDGAMAEADIIETLSLEKLVNENKCLCQIEPCSAVLGYGKKVDKSIKNGLNWLLNNIA 191
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARFRP_BOVIN (ADP-ribosylation factor-related protein 1 OS=Bos taurus GN=ARFRP1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.433e-18
Identity = 40/113 (35.40%), Postives = 64/113 (56.64%), Query Frame = 3 
Query:   87 LFSRLFAKKEMRILMVGLDAAGKTTIL----------YK-LKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L+  +F K E  +L++GLD AGKTT L          YK + L +I TT+   G N+ TV+        WD+GGQ++++ LW  Y+    G+I+V+DS D +R+ E++    +
Sbjct:    8 LYKYMFQKDEYCVLILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTV---GLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLSESKQAFEK 117

HSP 2 Score: 35.4242 bits (80), Expect = 1.433e-18
Identity = 18/66 (27.27%), Postives = 34/66 (51.52%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLG--LHSLRQRHWYIQSTCATSGEGLYEGLDWL 583
            M+  + L    +LV ANKQD+   ++  +I          + +R    Q+  A +G+G+ EG++W+
Sbjct:  118 MVTSEALDGVPILVLANKQDVETCLSIPDIKTAFSDCASKIGRRDCLTQACSALTGKGVREGIEWM 183
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARF_ENCCU (ADP-ribosylation factor OS=Encephalitozoon cuniculi GN=ARF-1 PE=1 SV=3)

HSP 1 Score: 93.2041 bits (230), Expect = 1.491e-18
Identity = 45/107 (42.06%), Postives = 63/107 (58.88%), Query Frame = 3 
Query:   72 LSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L +TKL      + E  I M+GLD AGKTT+L  L+ GE+  T+PT+GFN E V   ++ F VWD+GGQ+     W  Y     G+I++VD  D  R  ++ +EL R
Sbjct:   11 LLYTKLRGLFSGQSERSITMIGLDGAGKTTLLLYLQTGEVHQTVPTLGFNCENVTLGSMKFQVWDIGGQNSFMRFWHQYINEGCGIIYMVDCADPQRFGKSGEELWR 117
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARFRP_RAT (ADP-ribosylation factor-related protein 1 OS=Rattus norvegicus GN=Arfrp1 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.864e-18
Identity = 41/113 (36.28%), Postives = 65/113 (57.52%), Query Frame = 3 
Query:   87 LFSRLFAKKEMRILMVGLDAAGKTTIL----------YK-LKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L+  +F K E  IL++GLD AGKTT L          YK + L +I TT+   G N+ TV+        WD+GGQ++++ LW  Y+    G+I+V+DS D +R+ E+++   +
Sbjct:    8 LYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTV---GLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLSESKEAFEK 117

HSP 2 Score: 33.8834 bits (76), Expect = 1.864e-18
Identity = 20/71 (28.17%), Postives = 38/71 (53.52%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEI-------TDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWL 583
            +++ + L    +LV ANKQD+   ++  +I       T K+G     +R    Q+  A +G+G+ EG++W+
Sbjct:  118 VVSSEALDGVPILVLANKQDVETCLSIPDIKTAFSDCTCKIG-----RRDCLTQACSALTGKGVREGIEWM 183
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARFRP_PONAB (ADP-ribosylation factor-related protein 1 OS=Pongo abelii GN=ARFRP1 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.864e-18
Identity = 41/113 (36.28%), Postives = 64/113 (56.64%), Query Frame = 3 
Query:   87 LFSRLFAKKEMRILMVGLDAAGKTTIL----------YK-LKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L+  +F K E  IL++GLD AGKTT L          YK + L +I TT+   G N+ TV+        WD+GGQ++++ LW  Y+    G+I+V+DS D +R+ E++    +
Sbjct:    8 LYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTV---GLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLAESKQAFEK 117

HSP 2 Score: 33.8834 bits (76), Expect = 1.864e-18
Identity = 20/71 (28.17%), Postives = 37/71 (52.11%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEI-------TDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWL 583
            ++  + L    +LV ANKQD+   ++  +I       T K+G     +R    Q+  A +G+G+ EG++W+
Sbjct:  118 VVTSEALCGVPVLVLANKQDVETCLSIPDIKTAFSDCTSKIG-----RRDCLTQACSALTGKGVREGIEWM 183
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARFRP_MOUSE (ADP-ribosylation factor-related protein 1 OS=Mus musculus GN=Arfrp1 PE=2 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 1.864e-18
Identity = 41/113 (36.28%), Postives = 65/113 (57.52%), Query Frame = 3 
Query:   87 LFSRLFAKKEMRILMVGLDAAGKTTIL----------YK-LKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L+  +F K E  IL++GLD AGKTT L          YK + L +I TT+   G N+ TV+        WD+GGQ++++ LW  Y+    G+I+V+DS D +R+ E+++   +
Sbjct:    8 LYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTV---GLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLSESKEAFEK 117

HSP 2 Score: 33.8834 bits (76), Expect = 1.864e-18
Identity = 20/71 (28.17%), Postives = 38/71 (53.52%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEI-------TDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWL 583
            +++ + L    +LV ANKQD+   ++  +I       T K+G     +R    Q+  A +G+G+ EG++W+
Sbjct:  118 VVSSEALDGVPILVLANKQDVETCLSIPDIKTAFSDCTCKIG-----RRDCLTQACSALTGKGVREGIEWM 183
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: ARFRP_HUMAN (ADP-ribosylation factor-related protein 1 OS=Homo sapiens GN=ARFRP1 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.864e-18
Identity = 41/113 (36.28%), Postives = 64/113 (56.64%), Query Frame = 3 
Query:   87 LFSRLFAKKEMRILMVGLDAAGKTTIL----------YK-LKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHR 392
            L+  +F K E  IL++GLD AGKTT L          YK + L +I TT+   G N+ TV+        WD+GGQ++++ LW  Y+    G+I+V+DS D +R+ E++    +
Sbjct:    8 LYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTV---GLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLAESKQAFEK 117

HSP 2 Score: 33.8834 bits (76), Expect = 1.864e-18
Identity = 20/71 (28.17%), Postives = 37/71 (52.11%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEI-------TDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWL 583
            ++  + L    +LV ANKQD+   ++  +I       T K+G     +R    Q+  A +G+G+ EG++W+
Sbjct:  118 VVTSEALCGVPVLVLANKQDVETCLSIPDIKTAFSDCTSKIG-----RRDCLTQACSALTGKGVREGIEWM 183
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: SAR1_PICST (Small COPII coat GTPase SAR1 OS=Pichia stipitis GN=SAR1 PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.428e-18
Identity = 36/92 (39.13%), Postives = 57/92 (61.96%), Query Frame = 3 
Query:  111 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDEL 386
            K  ++L +GLD AGKTT+L+ LK   + T  PT+    E +   ++ FT +D+GG  + R LW+ YF    G++F+VD+ D +R  E++ EL
Sbjct:   19 KHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGSVRFTTFDLGGHQQARRLWKDYFPEVNGIVFLVDAADPERFAESKAEL 110

HSP 2 Score: 33.113 bits (74), Expect = 2.428e-18
Identity = 24/76 (31.58%), Postives = 32/76 (42.11%), Query Frame = 2 
Query:  404 DELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL------------RQRHWYIQSTCATSGEGLYEGLDWLSNNI 595
            +EL     L+  NK D+P A+   E+   LGL++             R    Y+ S    SG G  EG  WLS  I
Sbjct:  117 EELSHVPFLILGNKIDVPTAVGEMELKSALGLYNTTGKDTGKLPEGSRPIEVYMVSVVMRSGYG--EGFKWLSQYI 190
[Close]
BLAST of CK936838 vs. ExPASy Swiss-Prot
Match: SAR1_TRIRE (Small COPII coat GTPase sar1 OS=Trichoderma reesei GN=sar1 PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.158e-18
Identity = 36/92 (39.13%), Postives = 56/92 (60.87%), Query Frame = 3 
Query:  111 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDEL 386
            K  ++L +GLD AGKTT+L+ LK   +    PT+    E +   N+ F  +D+GG  + R +WR YF +  G++F+VD+ D +R  EA+ EL
Sbjct:   19 KHAKLLFLGLDNAGKTTLLHMLKNDRVAILQPTLHPTSEELAIGNVRFNTFDLGGHQQARRIWRDYFPDVNGVVFLVDAKDHERFPEAKAEL 110

HSP 2 Score: 32.3426 bits (72), Expect = 3.158e-18
Identity = 21/79 (26.58%), Postives = 38/79 (48.10%), Query Frame = 2 
Query:  392 MLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLH-----------SLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 595
            +L+ +EL     ++  NK D P+A++  E+  +LGL+            +R    ++ S     G G  +G+ WLSN +
Sbjct:  113 LLSMEELSKVPFVILGNKIDHPDAVSEDELRHQLGLYQTTGKGRVQLEGIRPIELFMCSVVMRQGYG--DGIRWLSNYV 189
The following BLAST results are available for this feature:
BLAST of CK936838 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 216
Match NameE-valueIdentityDescription
SAR1_CANGA5.021e-1945.65Small COPII coat GTPase SAR1 OS=Candida glabrata G... [more]
AR13B_DANRE1.408e-1834.78ADP-ribosylation factor-like protein 13B OS=Danio ... [more]
ARFRP_BOVIN1.433e-1835.40ADP-ribosylation factor-related protein 1 OS=Bos t... [more]
ARF_ENCCU1.491e-1842.06ADP-ribosylation factor OS=Encephalitozoon cunicul... [more]
ARFRP_RAT1.864e-1836.28ADP-ribosylation factor-related protein 1 OS=Rattu... [more]
ARFRP_PONAB1.864e-1836.28ADP-ribosylation factor-related protein 1 OS=Pongo... [more]
ARFRP_MOUSE1.864e-1836.28ADP-ribosylation factor-related protein 1 OS=Mus m... [more]
ARFRP_HUMAN1.864e-1836.28ADP-ribosylation factor-related protein 1 OS=Homo ... [more]
SAR1_PICST2.428e-1839.13Small COPII coat GTPase SAR1 OS=Pichia stipitis GN... [more]
SAR1_TRIRE3.158e-1839.13Small COPII coat GTPase sar1 OS=Trichoderma reesei... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_E05 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_E05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936838 ID=CK936838; Name=CK936838; organism=Citrus sinensis; type=EST; length=720bp
GGGGACCATTCTCCTCGTTTTCTCTCAAGCAGCCTCCTCCTTCAGATCTC
GTGAGATAACAGAAAATGGGGCTGTCATTTACGAAACTTTTTAGCCGGCT
TTTTGCCAAGAAAGAGATGCGAATTCTGATGGTGGGTCTCGACGCTGCTG
GTAAGACCACCATTTTGTACAAGCTCAAGCTCGGTGAGATTGTGACAACC
ATTCCTACCATTGGTTTCAACGTGGAGACTGTGGAATATAAGAACATTAG
CTTCACTGTTTGGGATGTTGGGGGTCAGGATAAGATTCGTCCTTTGTGGA
GACATTACTTCCAAAACACTCAAGGACTTATCTTTGTGGTTGATAGCAAT
GATCGTGACCGTGTGGTTGAAGCTAGAGATGAACTGCACCGATGCTGAAT
GAGGATGAGTTGAGGGATGCTGTGCTGCTTGTTTTTGCCAACAAGCAAGA
TCTTCCAAATGCCATGAATGCTGCAGAGATCACTGATAAGCTTGGTCTTC
ACTCACTTCGCCAACGCCACTGGTACATCCAGAGCACATGTGCAACATCT
GGGGAAGGACTCTATGAGGGTCTGGACTGGCTCTCCAACAACATCGCTAA
CAAGGCTTAAGTGATTGGTCAAGTCTGAGCGACTCTTTGCGGCAGCATCT
ATGTGAATGATGTTACTATGCTTTTGTTTATTTTGTCGGGGATTCTTTTT
GGGCTTTACTACCTACGTTN
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