CK936734

Overview
NameCK936734
Unique NameCK936734
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length820
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_NEIMB (Thymidylate synthase OS=Neisseria meningitidis serogroup B GN=thyA PE=3 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.513e-47
Identity = 86/149 (57.72%), Postives = 114/149 (76.51%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            + +IK NP+ RR+++SAWNP+ +  MALPPCH   +FY+A+G+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A VC L  G+F+H  GDAH+YRNH      QL++ P+  P++KINPE KD+ SF  EDF+L GYDPH  I+  ++V
Sbjct:  116 LEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_DECAR (Thymidylate synthase OS=Dechloromonas aromatica (strain RCB) GN=thyA PE=3 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 4.287e-47
Identity = 86/149 (57.72%), Postives = 109/149 (73.15%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            +N +KNNP+ RR ++SAWNP D+  MALPPCH   +FY+ANG+LSCQ+YQRSAD+ LGVPFNIASYAL T M+A VC   PG+F+H  GDAH+Y NH    + QL +  +P P + INPE KD+ +F  EDF+L GYDPH  I   +AV
Sbjct:  116 VNSLKNNPDSRRHIVSAWNPGDVDNMALPPCHCLFQFYVANGKLSCQLYQRSADIFLGVPFNIASYALFTMMLAQVCGYQPGEFVHTFGDAHLYSNHFEQAKLQLSRDTRPLPTMWINPEVKDLFAFRFEDFRLEGYDPHPHIPAPVAV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_CYTH3 (Thymidylate synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN=thyA PE=3 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 4.287e-47
Identity = 86/149 (57.72%), Postives = 112/149 (75.17%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            +N IKNNP+ RR+++SAWN  +++ M LPPCH F +FY+A+G+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A VC L  GDF+H +GDAH+Y NH+   + QL +  +P P +KINP+ K I  F  EDF L GYDPH  I+  +AV
Sbjct:  116 VNMIKNNPDSRRLIVSAWNVGEIEKMKLPPCHAFFQFYVADGKLSCQLYQRSADVFLGVPFNIASYALLTMMVAQVCGLQAGDFVHTLGDAHLYSNHLEQAKLQLSRDFRPLPAMKINPDVKSIFDFKFEDFTLEGYDPHPHIKAAVAV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_OLICO (Thymidylate synthase OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=thyA PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 5.599e-47
Identity = 86/149 (57.72%), Postives = 110/149 (73.83%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            +  I+ NP+ RR++++AWNP+D++ MALPPCH   +FY+ANG LSCQ+YQRSAD+ LGVPFNIASYALL  MIA V DLAPG F+H  GDAH+Y NH+     QL + P+  P L+INP  +DI  F  EDFKL  YDPH  I+ ++AV
Sbjct:  116 VQMIRTNPDSRRLIVTAWNPADVEQMALPPCHCLFQFYVANGRLSCQLYQRSADVFLGVPFNIASYALLMMMIAQVTDLAPGHFVHTFGDAHLYSNHLDQARLQLTRAPRALPTLRINPAVRDIFGFRYEDFKLENYDPHPHIKAEVAV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_NEIMA (Thymidylate synthase OS=Neisseria meningitidis serogroup A GN=thyA PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 5.599e-47
Identity = 85/149 (57.05%), Postives = 114/149 (76.51%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            + +IK NP+ RR+++SAWNP+ +  MALPPCH   +FY+A+G+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A VC L  G+F+H  GDAH+YRNH      QL++ P+  P++KINPE KD+ +F  EDF+L GYDPH  I+  ++V
Sbjct:  116 LEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_NEIG1 (Thymidylate synthase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=thyA PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 5.599e-47
Identity = 85/149 (57.05%), Postives = 114/149 (76.51%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            + +IK NP+ RR+++SAWNP+ +  MALPPCH   +FY+A+G+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A VC L  G+F+H  GDAH+YRNH      QL++ P+  P++KINPE KD+ +F  EDF+L GYDPH  I+  ++V
Sbjct:  116 VEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_NEIGO (Thymidylate synthase OS=Neisseria gonorrhoeae GN=thyA PE=3 SV=2)

HSP 1 Score: 187.578 bits (475), Expect = 7.312e-47
Identity = 85/149 (57.05%), Postives = 113/149 (75.84%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            + +IK NP+ RR+++SAWNP+ +  MALPPCH   +FY+A+G LSCQ+YQRSAD+ LGVPFNIASYALLT M+A VC L  G+F+H  GDAH+YRNH      QL++ P+  P++KINPE KD+ +F  EDF+L GYDPH  I+  ++V
Sbjct:  117 VEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGNLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV 265
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_ASCSU (Thymidylate synthase OS=Ascaris suum PE=2 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 7.312e-47
Identity = 91/149 (61.07%), Postives = 106/149 (71.14%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            I +IKNNPN RRI+L+AWN  DL  MALPPCH  A+F + NGELSCQ+YQRS DMGLGVPFN+ASY LLT MIAHVC L  G   HV+GDAHVY NH+  L+EQL + P+PFP +        ID F AE   L GY+P   I+M MAV
Sbjct:  146 IEQIKNNPNSRRIILNAWNVKDLHQMALPPCHTLAQFAVKNGELSCQLYQRSGDMGLGVPFNLASYGLLTHMIAHVCALKTGFLHHVLGDAHVYLNHIDALKEQLSRDPRPFPTVHFEGRIDSIDDFTAESIILNGYNPMAPIKMPMAV 294
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_NITWN (Thymidylate synthase OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=thyA PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.247e-46
Identity = 86/149 (57.72%), Postives = 112/149 (75.17%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            I+ I+ NP+ RR+++SAWNP+D+  MALPPCH   +FY+A G+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A V  L PGDF+H +GDAH+Y NH+     QL +  +P P + INPE KDI +F  EDF+L  YDPH  I+ ++AV
Sbjct:  116 IDLIQRNPDSRRLIVSAWNPADVDRMALPPCHCLFQFYVAGGKLSCQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFVHSLGDAHLYSNHLDQARLQLSRPTRPLPTMTINPEVKDIFAFRYEDFRLENYDPHPHIKAEVAV 264
[Close]
BLAST of CK936734 vs. ExPASy Swiss-Prot
Match: TYSY_EXISA (Thymidylate synthase OS=Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b) GN=thyA PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.247e-46
Identity = 85/149 (57.05%), Postives = 114/149 (76.51%), Query Frame = 1 
Query:    4 INKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFARFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVGPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 450
            I +IK NP+ RR+++SAWNP  L+ MALPPCH+  +FY+A+G+LSCQ+YQRSAD  LGVPFNIASYALLT M+AHV  L  GDF+H +GDAH+Y NH+  +E QL + P+P P L I  +   I+SF  EDF+++GYDPH  I+ +++V
Sbjct:  116 IEQIKTNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLSCQLYQRSADTFLGVPFNIASYALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIESFRFEDFEILGYDPHPHIKGEVSV 264
The following BLAST results are available for this feature:
BLAST of CK936734 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 406
Match NameE-valueIdentityDescription
TYSY_NEIMB2.513e-4757.72Thymidylate synthase OS=Neisseria meningitidis ser... [more]
TYSY_DECAR4.287e-4757.72Thymidylate synthase OS=Dechloromonas aromatica (s... [more]
TYSY_CYTH34.287e-4757.72Thymidylate synthase OS=Cytophaga hutchinsonii (st... [more]
TYSY_OLICO5.599e-4757.72Thymidylate synthase OS=Oligotropha carboxidovoran... [more]
TYSY_NEIMA5.599e-4757.05Thymidylate synthase OS=Neisseria meningitidis ser... [more]
TYSY_NEIG15.599e-4757.05Thymidylate synthase OS=Neisseria gonorrhoeae (str... [more]
TYSY_NEIGO7.312e-4757.05Thymidylate synthase OS=Neisseria gonorrhoeae GN=t... [more]
TYSY_ASCSU7.312e-4761.07Thymidylate synthase OS=Ascaris suum PE=2 SV=1[more]
TYSY_NITWN1.247e-4657.72Thymidylate synthase OS=Nitrobacter winogradskyi (... [more]
TYSY_EXISA1.247e-4657.05Thymidylate synthase OS=Exiguobacterium sp. (strai... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004518_G06 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IF_G06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936734 ID=CK936734; Name=CK936734; organism=Citrus sinensis; type=EST; length=820bp
GGGATTAACAAGATTAAGAATAATCCAAATGATCGGCGGATTGTACTCTC
GGCTTGGAATCCTTCTGATCTCAAATTGATGGCGCTTCCACCTTGCCACA
TGTTTGCTCGGTTCTATATGGCTAATGGGGAGTTATCATGTCAAATGTAT
CAGCGCTCTGCTGATATGGGCCTGGGTGTGCCGTTTAACATTGCTTCATA
TGCCCTCTTGACATGCATGATTGCTCATGTTTGTGATCTTGCTCCTGGCG
ATTTCATTCATGTCATTGGTGATGCTCATGTTTATCGCAATCATGTCGGG
CCCCTGGAGGAACAACTTCAGAAACTGCCAAAGCCTTTTCCAATTTTGAA
GATCAATCCTGAGAAGAAAGATATAGATTCTTTTGTAGCAGAGGATTTCA
AACTTATAGGTTATGATCCACACCAGAAAATAGAAATGAAAATGGCGGTA
TAGATCTCTCTTATAGGTTCAGTAACTCACAGACTCTGGTCCCTCCTAGG
CAATAATTGCCAAGTTTTGAGGCACCGCTACTGGAGGCATGTATTGACGC
AGAGTTTTATACAAATAGCCAATAAGAATCTGATTGTTGGATAAACTTTT
AAATAGTGCCTATGTAGTAAGCGGGAATCCTTTAGTCCACAAGAGTCGGT
GGTTCTAAGGTGATTTAGGATGTTTGTATTTTCTTGCTTTTCAACTTTCA
TTTTCCCTTGTTACATGAAAACTATCACAGTATGTACTTCTGCTCNAAGT
CTCGAATGCTCTTTTTGAAAGAGATTNNNNNNCTTGGATGTAAAAAAAAA
AAAAAAAAAAAAAAAAAAAA
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