CK936525

Overview
NameCK936525
Unique NameCK936525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length840
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSYIN (NAD-dependent malic enzyme OS=Psychromonas ingrahamii (strain 37) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.650e-25
Identity = 53/138 (38.41%), Postives = 96/138 (69.57%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS++ERN+  L GLLP  + + E Q  +    +  ++  L K++ + ++Q+ N+ LF+ L+ +++EE++P++YTPTVG AC+++  IY R +G+F+S  ++ K+ ++L+N  ++N++VIVVT
Sbjct:   12 AGPALLESPLLNKGSAFSQEERNNFNLTGLLPHNIETIESQSTRAYQQLSSFKSDLDKHIYLRNIQDTNETLFHHLIENHLEEVMPLIYTPTVGLACEQFSKIYRRKRGLFVSYPERHKIDDMLQNATKQNVKVIVVT 149
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_EDWI9 (NAD-dependent malic enzyme OS=Edwardsiella ictaluri (strain 93-146) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.650e-25
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFSE+ERN+  L GLLP  + + E QV++       +    ++++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FI+  ++ ++ ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFSEEERNTFNLNGLLPEAIETIEEQVERAYRQFCDFHSATEQHIYLRNIQDTNETLFYRLLRSHLSEMMPIIYTPTVGEACEHFSYIYRRARGLFIAYPNRDRIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO12_VIBVY (NAD-dependent malic enzyme 2 OS=Vibrio vulnificus (strain YJ016) GN=sfcA2 PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.650e-25
Identity = 52/138 (37.68%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AF+ +ER +  L GLLP  + + + QV +       +   ++K++ + ++Q+ N+ L+++L+  ++EE++PI+YTPTVGEACQK+  IY R +G+F+S +D+ ++  +L N P  +++VIV+T
Sbjct:   10 AGNTLLNTPLLNKGSAFTLEERKNFNLIGLLPANIETIDEQVSRAYEQFSLFNSAMEKHIYLRNIQDTNETLYFRLINQHIEEMMPIIYTPTVGEACQKFSQIYRRNRGLFLSFEDQDELEAILNNAPNTHVKVIVIT 147
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PROMH (NAD-dependent malic enzyme OS=Proteus mirabilis (strain HI4320) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 4.767e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFSE+ER++  L GLLP  V + E QV++       ++    K++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FIS  ++  + ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFSEEERSTFNLHGLLPEAVETIEEQVERAYRQYLDFKNDNDKHIYLRNIQDTNETLFYRLLESHLTEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRANIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PECCP (NAD-dependent malic enzyme OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 4.767e-25
Identity = 55/138 (39.86%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AF+E+ER +  L GLLP  V + E Q ++     ++++  ++K++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FIS  ++  + ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERANFNLHGLLPEAVETIEEQAERAWRQYQEFKHDIEKHVYLRNIQDTNETLFYRLLDGHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRAHIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_KLEP7 (NAD-dependent malic enzyme OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 4.767e-25
Identity = 55/138 (39.86%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER++  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  N++VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERSNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLIGNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNLDDILQNVPNHNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBHB (NAD-dependent malic enzyme OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 6.226e-25
Identity = 53/138 (38.41%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER+S  L GLLP T  + + QV++     + ++  + K++ + ++Q+ N+ L+Y+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS  ++ ++ ++L N    N++VIVVT
Sbjct:   13 AGPALLATPLLNKGSAFSGEERSSFNLEGLLPETTETIQEQVERAYQQYKSFESDMDKHIYLRNIQDTNETLYYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYPNRDRIDDLLNNAANHNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_KLEP3 (NAD-dependent malic enzyme OS=Klebsiella pneumoniae (strain 342) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 6.226e-25
Identity = 55/138 (39.86%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER++  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  N++VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSLEERSNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLIGNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNLDDILQNVPNHNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ERWCT (NAD-dependent malic enzyme OS=Erwinia carotovora subsp. atroseptica GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 6.226e-25
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AF+E+ER    L GLLP  V + E Q ++     ++++  ++K++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FIS  ++  + ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERADFNLAGLLPEAVETIEEQAERAWRQYQEFKHDIEKHVYLRNIQDTNETLFYRLLDGHLNEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRANIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_CITK8 (NAD-dependent malic enzyme OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 6.226e-25
Identity = 56/138 (40.58%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSIEERRNFNLLGLLPEVVESIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLDEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
The following BLAST results are available for this feature:
BLAST of CK936525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_PSYIN3.650e-2538.41NAD-dependent malic enzyme OS=Psychromonas ingraha... [more]
MAO1_EDWI93.650e-2539.86NAD-dependent malic enzyme OS=Edwardsiella ictalur... [more]
MAO12_VIBVY3.650e-2537.68NAD-dependent malic enzyme 2 OS=Vibrio vulnificus ... [more]
MAO1_PROMH4.767e-2541.30NAD-dependent malic enzyme OS=Proteus mirabilis (s... [more]
MAO1_PECCP4.767e-2539.86NAD-dependent malic enzyme OS=Pectobacterium carot... [more]
MAO1_KLEP74.767e-2539.86NAD-dependent malic enzyme OS=Klebsiella pneumonia... [more]
MAO1_VIBHB6.226e-2538.41NAD-dependent malic enzyme OS=Vibrio harveyi (stra... [more]
MAO1_KLEP36.226e-2539.86NAD-dependent malic enzyme OS=Klebsiella pneumonia... [more]
MAO1_ERWCT6.226e-2539.86NAD-dependent malic enzyme OS=Erwinia carotovora s... [more]
MAO1_CITK86.226e-2540.58NAD-dependent malic enzyme OS=Citrobacter koseri (... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004521_C09 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_C09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936525 ID=CK936525; Name=CK936525; organism=Citrus sinensis; type=EST; length=840bp
GGGGAGACCCNNNAGACTCTCCCTTCTCTTCTCATTGCCTCTGCAACTTT
TTTTTCTATTTTTACTTCGTCACACATTTTTAATCAGCGCAAGACTCTCT
CTTCACACATTTCTCTCGCCATGTTTTCGTTAAACAGAAGCGCTTTTCTG
AATAATCCTGCGAGCATATCAGGCTCGTTCCCTGATAAGCATAGAAGAAT
ACTGCCTACAAGAGTGGTGGTTGCGGGCTCGTCTAACAGAGATAAGAGTA
ACGGTAGTCTTTTAATGGCAACCGATGAAAGCACAGCTACATTAAAAGAA
ATGAGAGATGGCTATGCCGAAGTTGTGGACCCCAAGTCCGGCGTCAGCGG
TGGGGTCCAGGACGTTTATGGTGAGGATACCGCCACCGAAGACCAGCCGG
TCACCCCTTGGTCTGTCTCAGTTGCTAGTGGGTATTCTTTGTTGAGAGAT
CCACACCACAACAAAGGGCTTGCCTTCTCTGAAAAAGAAAGGAATTCACA
TTACTTGCGTGGTCTTCTCCCCCCTACAGTTATATCCCAAGAGCTCCAGG
TGAAGAAAATGTTGCATAATATTCGGCAGTACCAAGTTCCACTCCAGAAG
TACATGGCCATGATGGATCTTCAGGAGAGAAATCAGAAACTGTTCTATAA
GCTTCTCATTGACAATGTTGAGGAGTTACTCCCAATTGTCTATACTCCAA
CTGTTGGAGAAGCTTGCCAGAAGTACGGGAGCATCTATAGTCGTCCTCAG
GGTGTTTTTATCAGTTTGAAGGATAAGGGCAAGGTTCTTGAGGTATTGAG
GAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACT
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