CV887373

Overview
NameCV887373
Unique NameCV887373
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length973
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Library UCRCS04-UCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_MYCSS (Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (strain MCS) GN=pth PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.912e-20
Identity = 47/130 (36.15%), Postives = 75/130 (57.69%), Query Frame = -2 
Query:  460 RFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGL 849
            R  G A V+LAKP+TYMN SG + GPLA +Y +    V+V HD++ +  G               LKSV     G ++F R+R+G+GRPPG+ D   ++L+ F ++ R  + T  ++  +  +LL+++GL
Sbjct:   54 RLAGRA-VVLAKPRTYMNESGRNVGPLAKFYSVAPADVIVIHDELDIDFGRIRLKFGGGVAGHNGLKSVAAAL-GTKDFQRVRVGVGRPPGRKDAATYVLEPFTSVERPEVPTICEQAADATELLIAQGL 181

HSP 2 Score: 29.6462 bits (65), Expect = 8.912e-20
Identity = 13/23 (56.52%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  903 GNPGDKYKGTRPNVGFEMIDAFA 971
            GNPG  Y  TR NVGF + D  A
Sbjct:   11 GNPGPNYAKTRHNVGFVVADLLA 33
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_MYCSK (Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (strain KMS) GN=pth PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.912e-20
Identity = 47/130 (36.15%), Postives = 75/130 (57.69%), Query Frame = -2 
Query:  460 RFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGL 849
            R  G A V+LAKP+TYMN SG + GPLA +Y +    V+V HD++ +  G               LKSV     G ++F R+R+G+GRPPG+ D   ++L+ F ++ R  + T  ++  +  +LL+++GL
Sbjct:   54 RLAGRA-VVLAKPRTYMNESGRNVGPLAKFYSVAPADVIVIHDELDIDFGRIRLKFGGGVAGHNGLKSVAAAL-GTKDFQRVRVGVGRPPGRKDAATYVLEPFTSVERPEVPTICEQAADATELLIAQGL 181

HSP 2 Score: 29.6462 bits (65), Expect = 8.912e-20
Identity = 13/23 (56.52%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  903 GNPGDKYKGTRPNVGFEMIDAFA 971
            GNPG  Y  TR NVGF + D  A
Sbjct:   11 GNPGPNYAKTRHNVGFVVADLLA 33
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_MYCSJ (Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (strain JLS) GN=pth PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.912e-20
Identity = 47/130 (36.15%), Postives = 75/130 (57.69%), Query Frame = -2 
Query:  460 RFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGL 849
            R  G A V+LAKP+TYMN SG + GPLA +Y +    V+V HD++ +  G               LKSV     G ++F R+R+G+GRPPG+ D   ++L+ F ++ R  + T  ++  +  +LL+++GL
Sbjct:   54 RLAGRA-VVLAKPRTYMNESGRNVGPLAKFYSVAPADVIVIHDELDIDFGRIRLKFGGGVAGHNGLKSVAAAL-GTKDFQRVRVGVGRPPGRKDAATYVLEPFTSVERPEVPTICEQAADATELLIAQGL 181

HSP 2 Score: 29.6462 bits (65), Expect = 8.912e-20
Identity = 13/23 (56.52%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  903 GNPGDKYKGTRPNVGFEMIDAFA 971
            GNPG  Y  TR NVGF + D  A
Sbjct:   11 GNPGPNYAKTRHNVGFVVADLLA 33
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_BACHK (Peptidyl-tRNA hydrolase OS=Bacillus thuringiensis subsp. konkukian GN=pth PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.924e-20
Identity = 44/142 (30.99%), Postives = 73/142 (51.41%), Query Frame = -2 
Query:  433 FRTRFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNT 858
            F   FV    V+L KP TYMNLSGES  PL  YYK+ +   +V +DD+ +P G               +KS +++  G +EF R+R+GI RP   M    ++L +F +     ++ ++++  +  +  L+K   +    FN+
Sbjct:   46 FGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHL-GTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQIMNTFNS 186

HSP 2 Score: 41.9726 bits (97), Expect = 8.924e-20
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNPG +Y+ TR N+GF  ID  A+   IS+N    K  FG G +
Sbjct:    8 GNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGLFGAGFV 51
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_BACAN (Peptidyl-tRNA hydrolase OS=Bacillus anthracis GN=pth PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.924e-20
Identity = 44/142 (30.99%), Postives = 73/142 (51.41%), Query Frame = -2 
Query:  433 FRTRFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNT 858
            F   FV    V+L KP TYMNLSGES  PL  YYK+ +   +V +DD+ +P G               +KS +++  G +EF R+R+GI RP   M    ++L +F +     ++ ++++  +  +  L+K   +    FN+
Sbjct:   46 FGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHL-GTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQIMNTFNS 186

HSP 2 Score: 41.9726 bits (97), Expect = 8.924e-20
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNPG +Y+ TR N+GF  ID  A+   IS+N    K  FG G +
Sbjct:    8 GNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGLFGAGFV 51
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_MANSM (Peptidyl-tRNA hydrolase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=pth PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.159e-19
Identity = 48/136 (35.29%), Postives = 70/136 (51.47%), Query Frame = -2 
Query:  436 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 843
            +G   V    P T+MNLSG++ G LA +Y++    +LV HD++ LP GV              LK  +     N+ F RLRIGIG P  +    A++L K + I R  ID AL E    +++LL  G+T++    N
Sbjct:   55 IGGEEVRFLIPTTFMNLSGKAVGALATFYRIKPEEILVIHDELDLPPGVAKIKQGGGHGGHNGLKDTIAQLANNKNFYRLRIGIGHPGDKNLVSAYVLSKPSPIDRSAIDKALDEAASCMEILLKDGITKATNRLN 190

HSP 2 Score: 29.6462 bits (65), Expect = 1.159e-19
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = -3 
Query:  903 GNPGDKYKGTRPNVGFEMIDAFA 971
            GNPGDKY  TR N G  +++  A
Sbjct:   11 GNPGDKYADTRHNAGEWLVERLA 33
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_GEOKA (Peptidyl-tRNA hydrolase OS=Geobacillus kaustophilus GN=pth PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.161e-19
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -2 
Query:  436 FRTRFVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 858
            F T  V    V L KP TYMNLSGE   PL  YY + ++ V+V +DD+ LP G               +KS++++  G  +F R+RIGIGRP G      ++L +F    +  +D A+    +  +  +     +    FN
Sbjct:   46 FGTASVSGEKVALCKPLTYMNLSGECVRPLMDYYDIAIDDVIVIYDDLDLPPGKIRLRLKGSSGGHNGVKSLIHHL-GTEQFKRIRIGIGRPAGGQPVTDYVLGRFTEEEKPAVDKAVLRAADACEQAVKAPFIQVMNDFN 185

HSP 2 Score: 40.817 bits (94), Expect = 1.161e-19
Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = -3 
Query:  852 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFG 971
            GNPG +Y+ TR NVGF +ID  A+   +S+ T   +  FG
Sbjct:    8 GNPGKEYEQTRHNVGFFVIDELAKRWNVSLKTAKFRGLFG 47
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_MOOTA (Peptidyl-tRNA hydrolase OS=Moorella thermoacetica (strain ATCC 39073) GN=pth PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.552e-19
Identity = 49/137 (35.77%), Postives = 69/137 (50.36%), Query Frame = -2 
Query:  436 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKG-LTESARHFN 843
            VG+  VLL KPQTYMN SG++  PLA +Y +    +LV HDD+ L  G               L+S++ +  G     RL+IGIGRPP   +   ++L+ F+    E I   L E     + LL  G + E+   FN
Sbjct:   54 VGNNRVLLVKPQTYMNNSGQAVAPLARWYGIAPEAILVIHDDLDLAPGRLRLRRGGSSGGHRGLQSIITHL-GTTAVPRLKIGIGRPPLGQNVIDYVLKPFSEGDWELIRPVLLEAARAARFLLEGGSMDEAMNRFN 189

HSP 2 Score: 43.1282 bits (100), Expect = 2.552e-19
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNPG +Y  TR N GF ++D  A+  GI+++    +A  GQGL+
Sbjct:   11 GNPGPRYATTRHNAGFMVVDLLADDLGITLDRTREQALTGQGLV 54
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_BDEBA (Peptidyl-tRNA hydrolase OS=Bdellovibrio bacteriovorus GN=pth PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.554e-19
Identity = 45/138 (32.61%), Postives = 75/138 (54.35%), Query Frame = -2 
Query:  433 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDP-KAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNT 843
            + D PV+  KPQTYMNLSGES  PL  +YK+PL R++V HD++  P                 +KS+ +   G+ ++ RLR+G+GRP     P   ++L KF      ++   L +  + ++ ++  G+ +++  FNT
Sbjct:   51 IKDHPVIFCKPQTYMNLSGESVQPLMGFYKIPLERLIVIHDEIDQPFAQMKIQKNRGHGGHNGIKSI-SGLMGSMDYTRLRLGVGRPANPNIPVPDYVLGKFTKEEFAQMPDFLNKAGDAVESIILDGIQKASTKFNT 187

HSP 2 Score: 33.113 bits (74), Expect = 2.554e-19
Identity = 14/27 (51.85%), Postives = 17/27 (62.96%), Query Frame = -3 
Query:  891 GNPGDKYKGTRPNVGFEMIDAFAESQG 971
            GNPG +YK TR N+GF  +D   E  G
Sbjct:    8 GNPGGEYKLTRHNIGFMAVDFLMEGLG 34
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_ANADE (Peptidyl-tRNA hydrolase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=pth PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.317e-19
Identity = 47/136 (34.56%), Postives = 73/136 (53.68%), Query Frame = -2 
Query:  436 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 843
            +G   V + KPQTYMN SGE+ G    ++KL L+ ++V HDD+ L                  +KSV N   G+ E+AR+R+G+GRPP +MDP  ++L KF       +D  +++ VE  +L +  G  ++    N
Sbjct:   51 LGPERVWILKPQTYMNHSGEAVGAALRFWKLGLDDLVVVHDDLELEPFRVQLKVGGGHGGHNGVKSV-NAHVGSPEYARVRVGVGRPPPRMDPADYVLGKFAKGEDAELDLCVEQAVEATRLAVELGAAKAMNQVN 185

HSP 2 Score: 34.6538 bits (78), Expect = 3.317e-19
Identity = 17/44 (38.64%), Postives = 22/44 (50.00%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNPG++Y  TR NVGF + D  A+  G S       A   +  L
Sbjct:    8 GNPGEEYARTRHNVGFVVADRLAQLAGASFTAKKFAAELAEARL 51
The following BLAST results are available for this feature:
BLAST of CV887373 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PTH_MYCSS8.912e-2036.15Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (stra... [more]
PTH_MYCSK8.912e-2036.15Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (stra... [more]
PTH_MYCSJ8.912e-2036.15Peptidyl-tRNA hydrolase OS=Mycobacterium sp. (stra... [more]
PTH_BACHK8.924e-2030.99Peptidyl-tRNA hydrolase OS=Bacillus thuringiensis ... [more]
PTH_BACAN8.924e-2030.99Peptidyl-tRNA hydrolase OS=Bacillus anthracis GN=p... [more]
PTH_MANSM1.159e-1935.29Peptidyl-tRNA hydrolase OS=Mannheimia succinicipro... [more]
PTH_GEOKA1.161e-1931.91Peptidyl-tRNA hydrolase OS=Geobacillus kaustophilu... [more]
PTH_MOOTA2.552e-1935.77Peptidyl-tRNA hydrolase OS=Moorella thermoacetica ... [more]
PTH_BDEBA2.554e-1932.61Peptidyl-tRNA hydrolase OS=Bdellovibrio bacteriovo... [more]
PTH_ANADE3.317e-1934.56Peptidyl-tRNA hydrolase OS=Anaeromyxobacter dehalo... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_2_030H06_T7 Ruby Orange Developing Flower cDNA Library UCRCS04-UCR Citrus sinensis cDNA clone UCRCS04_030_T7_H06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV887373 ID=CV887373; Name=CV887373; organism=Citrus sinensis; type=EST; length=973bp
TGCAATATACATAATATAATAAAGTACACCAAATTGCACAAAACATGATA
TTTATAGGCCAGCAACGAAAGCTATATCATAAATTCCTAGAGGATTGACT
GTTTTGCTTTCATAATGGTCAGACCTTCAGTTGGCTACGACCATGACACT
GGGAAAAGTCTCCAAATTTGAGAAGTGCTGATTTGCTGCAAAATGAACAG
ACAAAGCAATTCAGTTTGCTAATTGGGCTAAGCCATTCACTTACGTCGTT
AGAAGTATTCTCTCATTGGGATGGGTGGTTTCGCCTCCTCTCTCCTCGGT
GATTTTAAATATTTGTATGTATTATACAAATGCGGCCATGAAGAACCATT
CGGTGCTTTTGTGGTTGTCATCGAAGGTACCAATAGCTCATGCTGGCATG
TTTTGCAATCTTATGTGCTTGTATTTTTGTATGGTGTTGAAATGTCTAGC
ACTCTCTGTCAAGCCTTTAGATAAAAGCAGCTTCAGAACCTCAACCCCTT
CTTGCAAAGCAGTATCAATTCGCTCTCGAGCTATTGCATTAAACTTCTGC
AGCAAGAAAGCTTTAGGATCCATTTGACCAGGAGGCCTCCCAATACCAAT
TCTTAGCCGAGCAAATTCTCGGTTACCTCGAAAGTTGTTCATCACACTCT
TCAGCCCATTGTGGCCTCCATGACCTCCATTGTGGCGCAGACGAAGCACT
CCACAAGGTAAGCCCATGTCATCATGAAACACAAGAACACGATTAAGAGG
TAACTTATAATAAGCTGCAAGTGGTCCAGTAGATTCACCGCTCAGATTCA
TGTAAGTCTGTGGCTTTGCAAGGAGTACAGGGGCATCACCAACAAACCTT
GTCCGAAAGTAGCTTTGCAATGAACCGTGTTCATAGAAATCCCTTGTGAT
TCAGCAAATGCGTCGATCATCTCAAACCCCACATTGGGTCTAGTTCCTTT
GTATTTATCTCCCGGGTTACCCA
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