CV887373

Overview
NameCV887373
Unique NameCV887373
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length973
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Library UCRCS04-UCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOK1 (Peptidyl-tRNA hydrolase OS=Clostridium kluyveri (strain NBRC 12016) GN=pth PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 5.619e-19
Identity = 44/137 (32.12%), Postives = 73/137 (53.28%), Query Frame = -2 
Query:  436 FVGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 846
            F+G   V+L KP TYMNLSGES      +YK+  +R+++ +DD+ +  G               +K+++ N  G   F R++IGIG P   +D  +++L KF+   R  I+  L+  VE L+ ++  G+  +   FN
Sbjct:   50 FIGQNKVILLKPGTYMNLSGESVREAVNFYKIENDRMIIIYDDIDIDIGRFRIRHRGSAGGHNGIKNIILNL-GTDVFPRIKIGIGHP--NVDLVSYVLGKFSEDDRINIEKVLKTSVEALECIMDFGIEVAMNKFN 183

HSP 2 Score: 40.0466 bits (92), Expect = 5.619e-19
Identity = 17/44 (38.64%), Postives = 26/44 (59.09%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GN G KY  TR N+GF+ ID  ++   I++N    K  +G+G +
Sbjct:    8 GNIGVKYHRTRHNIGFDFIDLVSKKYNITLNREKFKGNYGEGFI 51
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_DEIGD (Peptidyl-tRNA hydrolase OS=Deinococcus geothermalis (strain DSM 11300) GN=pth PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 7.269e-19
Identity = 49/139 (35.25%), Postives = 76/139 (54.68%), Query Frame = -2 
Query:  436 VGDAP---VLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 843
            +G AP   VLL KPQT+MN SG + GPL A+YKL    +LV  DD+  P G+              ++ ++    G ++FARL+IGI RPP   +P  ++L ++ A   E +   ++ G + +++ +S GL E    FN
Sbjct:   50 MGAAPGEKVLLVKPQTFMNSSGRAVGPLFAFYKLEPAALLVVQDDLDSPFGLLKFRLGGRHGGQNGVRDII-RVLGTQDFARLKIGISRPPAGWNPADWVLSRWRAEEAETLAELVRLGADAVEVWVSHGLAEGQARFN 187

HSP 2 Score: 30.4166 bits (67), Expect = 7.269e-19
Identity = 13/27 (48.15%), Postives = 17/27 (62.96%), Query Frame = -3 
Query:  891 GNPGDKYKGTRPNVGFEMIDAFAESQG 971
            GNPG +Y  TR NVG+ ++D  A   G
Sbjct:    8 GNPGSQYAQTRHNVGWLVVDEVARRWG 34
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_BREBN (Peptidyl-tRNA hydrolase OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) GN=pth PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.302e-19
Identity = 43/135 (31.85%), Postives = 68/135 (50.37%), Query Frame = -2 
Query:  424 VLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQK 828
            VLL KPQTYMNLSGES   +  +YKL  + ++V +DD+ LP G               +KS++ +  G +EF R+++GI RP        ++L  F    R  I  A+    +   +   +   +   HFN+++K
Sbjct:   56 VLLVKPQTYMNLSGESVAEVLKFYKLIPDDLVVIYDDLDLPTGHLRLREKGSAGGHNGIKSMIQHL-GTQEFKRIKVGISRPEPGRSVSDYVLNTFPVAERADIQEAVSLAADACAMWTRESFLKVMNHFNSLKK 189

HSP 2 Score: 39.2762 bits (90), Expect = 7.302e-19
Identity = 18/42 (42.86%), Postives = 24/42 (57.14%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GNPG KY+ TR N GF  ID  ++  GI +     +A  G+G
Sbjct:    8 GNPGKKYEDTRHNAGFMAIDKISDKWGIPVTQNKFRALVGEG 49
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOTE (Peptidyl-tRNA hydrolase OS=Clostridium tetani GN=pth PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 9.503e-19
Identity = 44/139 (31.65%), Postives = 73/139 (52.52%), Query Frame = -2 
Query:  427 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQ 843
            + +  V+L KP TYMNLSGES   +  YY +P+  V+V +DD+ L  G               +KS++ N   + +F RLRIG+G+P  Q D  + +L KF     + I+  L+   E+   ++++G  E+   +N  +
Sbjct:   51 ISNEKVILLKPTTYMNLSGESLREIIEYYNIPITNVIVIYDDVDLEVGRLRIRTKGSAGGHNGIKSIIYNL-NSEDFIRLRIGVGKP--QRDMVSHVLGKFAKEDEKNIEEVLKIIPELAYTIINQGPQEAMNKYNNFR 186

HSP 2 Score: 40.0466 bits (92), Expect = 9.503e-19
Identity = 17/42 (40.48%), Postives = 23/42 (54.76%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GNPG +Y+ TR N+GFE +D  +    I +     K  FG G
Sbjct:    8 GNPGKEYEKTRHNIGFEAVDKISYEYNIPIKRERFKGVFGDG 49
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_SYNAS (Peptidyl-tRNA hydrolase OS=Syntrophus aciditrophicus (strain SB) GN=pth PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.233e-18
Identity = 43/131 (32.82%), Postives = 69/131 (52.67%), Query Frame = -2 
Query:  436 VLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 828
            VLLAKPQT+MNLSG+S   LA ++K+ +  VLV HDD+ LP GV              L S+ ++  G  EF R+R+GIG+P  +   + ++L+ F       +   +      +  ++S G+  +   +N
Sbjct:   56 VLLAKPQTFMNLSGKSVRKLADFFKIVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDSL-GGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPRIIVTARSAILEVISSGIKTAMNSYN 185

HSP 2 Score: 41.9726 bits (97), Expect = 1.233e-18
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNPG +Y+ TR N GF ++D  AE Q I M+     A +G+G++
Sbjct:    8 GNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVI 51
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOPH (Peptidyl-tRNA hydrolase OS=Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) GN=pth PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.235e-18
Identity = 43/136 (31.62%), Postives = 69/136 (50.74%), Query Frame = -2 
Query:  436 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFN 843
            +G   V+LAKPQTYMNLSGES   L  +YK+    ++V +DD+ L  G               +K+++ +  G+ EF R++IG+G  P   D   ++L +F       I  AL++  +  + +L  G   +   FN
Sbjct:   51 IGGEKVILAKPQTYMNLSGESVRELIDFYKVTKEEIIVIYDDISLDVGQLRIRTKGSAGGHNGIKNIIAHL-GSDEFCRIKIGVGDKPKNWDLADYVLARFPKEEEPAIREALEKVSKACETILRDGAKVAMNLFN 185

HSP 2 Score: 41.2022 bits (95), Expect = 1.235e-18
Identity = 18/44 (40.91%), Postives = 28/44 (63.64%), Query Frame = -3 
Query:  840 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQGLL 971
            GNP  +Y+ TR N+GF+ I   A+   IS++T   KA  G+G++
Sbjct:    8 GNPTREYEATRHNIGFDAITRLADDNNISLDTKKHKAICGKGMI 51
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOBL (Peptidyl-tRNA hydrolase OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=pth PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.606e-18
Identity = 40/139 (28.78%), Postives = 72/139 (51.80%), Query Frame = -2 
Query:  427 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQ 843
            +G+  ++L KP TYMNLSGES    A +YK+    ++V +DDM +  G               +K+++ +   +  F R+R+GIG+P   +    ++L KF+   RE I+  L    +    ++  G+TE+   +N ++
Sbjct:   51 IGNEKIILLKPSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRGKGSAGGHNGIKNIIQHLNSD-IFPRVRVGIGQPDENV--VNYVLGKFSKDQREIIEKVLAMSAKACISIVEDGVTEAMNKYNGVK 186

HSP 2 Score: 43.5134 bits (101), Expect = 1.606e-18
Identity = 18/42 (42.86%), Postives = 27/42 (64.29%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GN G +YK TR N+GF+++D  AE   I +N    K ++G+G
Sbjct:    8 GNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEG 49
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOBJ (Peptidyl-tRNA hydrolase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=pth PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.606e-18
Identity = 40/139 (28.78%), Postives = 72/139 (51.80%), Query Frame = -2 
Query:  427 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQ 843
            +G+  ++L KP TYMNLSGES    A +YK+    ++V +DDM +  G               +K+++ +   +  F R+R+GIG+P   +    ++L KF+   RE I+  L    +    ++  G+TE+   +N ++
Sbjct:   51 IGNEKIILLKPSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRGKGSAGGHNGIKNIIQHLNSD-IFPRVRVGIGQPDENV--VNYVLGKFSKDEREIIEKVLAMSAKACISIVEDGVTEAMNKYNGVK 186

HSP 2 Score: 43.5134 bits (101), Expect = 1.606e-18
Identity = 18/42 (42.86%), Postives = 27/42 (64.29%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GN G +YK TR N+GF+++D  AE   I +N    K ++G+G
Sbjct:    8 GNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEG 49
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOBH (Peptidyl-tRNA hydrolase OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) GN=pth PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.606e-18
Identity = 40/139 (28.78%), Postives = 72/139 (51.80%), Query Frame = -2 
Query:  427 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQ 843
            +G+  ++L KP TYMNLSGES    A +YK+    ++V +DDM +  G               +K+++ +   +  F R+R+GIG+P   +    ++L KF+   RE I+  L    +    ++  G+TE+   +N ++
Sbjct:   51 IGNEKIILLKPSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQHLNSD-IFPRVRVGIGQPDENV--VNYVLGKFSKDEREIIEKVLAMSAKACISIVEDGVTEAMNKYNGVK 186

HSP 2 Score: 43.5134 bits (101), Expect = 1.606e-18
Identity = 18/42 (42.86%), Postives = 27/42 (64.29%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GN G +YK TR N+GF+++D  AE   I +N    K ++G+G
Sbjct:    8 GNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEG 49
[Close]
BLAST of CV887373 vs. ExPASy Swiss-Prot
Match: PTH_CLOB6 (Peptidyl-tRNA hydrolase OS=Clostridium botulinum (strain 657 / Type Ba4) GN=pth PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.606e-18
Identity = 40/139 (28.78%), Postives = 72/139 (51.80%), Query Frame = -2 
Query:  427 VGDAPVLLAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDMGLPCGVXXXXXXXXXXXXXXLKSVMNNFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNAIARERIDTALQEGVEVLKLLLSKGLTESARHFNTIQ 843
            +G+  ++L KP TYMNLSGES    A +YK+    ++V +DDM +  G               +K+++ +   +  F R+R+GIG+P   +    ++L KF+   RE I+  L    +    ++  G+TE+   +N ++
Sbjct:   51 IGNEKIILLKPSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRGKGSAGGHNGIKNIIQHLNSD-IFPRVRVGIGQPDENV--VNYVLGKFSKDQREIIEKVLAMSAKACISIVEDGVTEAMNKYNGVK 186

HSP 2 Score: 43.5134 bits (101), Expect = 1.606e-18
Identity = 18/42 (42.86%), Postives = 27/42 (64.29%), Query Frame = -3 
Query:  846 GNPGDKYKGTRPNVGFEMIDAFAESQGISMNTVHCKATFGQG 971
            GN G +YK TR N+GF+++D  AE   I +N    K ++G+G
Sbjct:    8 GNIGKEYKQTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEG 49
The following BLAST results are available for this feature:
BLAST of CV887373 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PTH_CLOK15.619e-1932.12Peptidyl-tRNA hydrolase OS=Clostridium kluyveri (s... [more]
PTH_DEIGD7.269e-1935.25Peptidyl-tRNA hydrolase OS=Deinococcus geothermali... [more]
PTH_BREBN7.302e-1931.85Peptidyl-tRNA hydrolase OS=Brevibacillus brevis (s... [more]
PTH_CLOTE9.503e-1931.65Peptidyl-tRNA hydrolase OS=Clostridium tetani GN=p... [more]
PTH_SYNAS1.233e-1832.82Peptidyl-tRNA hydrolase OS=Syntrophus aciditrophic... [more]
PTH_CLOPH1.235e-1831.62Peptidyl-tRNA hydrolase OS=Clostridium phytofermen... [more]
PTH_CLOBL1.606e-1828.78Peptidyl-tRNA hydrolase OS=Clostridium botulinum (... [more]
PTH_CLOBJ1.606e-1828.78Peptidyl-tRNA hydrolase OS=Clostridium botulinum (... [more]
PTH_CLOBH1.606e-1828.78Peptidyl-tRNA hydrolase OS=Clostridium botulinum (... [more]
PTH_CLOB61.606e-1828.78Peptidyl-tRNA hydrolase OS=Clostridium botulinum (... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_2_030H06_T7 Ruby Orange Developing Flower cDNA Library UCRCS04-UCR Citrus sinensis cDNA clone UCRCS04_030_T7_H06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV887373 ID=CV887373; Name=CV887373; organism=Citrus sinensis; type=EST; length=973bp
TGCAATATACATAATATAATAAAGTACACCAAATTGCACAAAACATGATA
TTTATAGGCCAGCAACGAAAGCTATATCATAAATTCCTAGAGGATTGACT
GTTTTGCTTTCATAATGGTCAGACCTTCAGTTGGCTACGACCATGACACT
GGGAAAAGTCTCCAAATTTGAGAAGTGCTGATTTGCTGCAAAATGAACAG
ACAAAGCAATTCAGTTTGCTAATTGGGCTAAGCCATTCACTTACGTCGTT
AGAAGTATTCTCTCATTGGGATGGGTGGTTTCGCCTCCTCTCTCCTCGGT
GATTTTAAATATTTGTATGTATTATACAAATGCGGCCATGAAGAACCATT
CGGTGCTTTTGTGGTTGTCATCGAAGGTACCAATAGCTCATGCTGGCATG
TTTTGCAATCTTATGTGCTTGTATTTTTGTATGGTGTTGAAATGTCTAGC
ACTCTCTGTCAAGCCTTTAGATAAAAGCAGCTTCAGAACCTCAACCCCTT
CTTGCAAAGCAGTATCAATTCGCTCTCGAGCTATTGCATTAAACTTCTGC
AGCAAGAAAGCTTTAGGATCCATTTGACCAGGAGGCCTCCCAATACCAAT
TCTTAGCCGAGCAAATTCTCGGTTACCTCGAAAGTTGTTCATCACACTCT
TCAGCCCATTGTGGCCTCCATGACCTCCATTGTGGCGCAGACGAAGCACT
CCACAAGGTAAGCCCATGTCATCATGAAACACAAGAACACGATTAAGAGG
TAACTTATAATAAGCTGCAAGTGGTCCAGTAGATTCACCGCTCAGATTCA
TGTAAGTCTGTGGCTTTGCAAGGAGTACAGGGGCATCACCAACAAACCTT
GTCCGAAAGTAGCTTTGCAATGAACCGTGTTCATAGAAATCCCTTGTGAT
TCAGCAAATGCGTCGATCATCTCAAACCCCACATTGGGTCTAGTTCCTTT
GTATTTATCTCCCGGGTTACCCA
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