EY720682 | Citrus Genome Database

Overview


Name	EY720682
Unique Name	EY720682
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	774

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FNSI_PETCR (Flavone synthase OS=Petroselinum crispum GN=FNSI PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.761e-13
Identity = 33/110 (30.00%), Postives = 56/110 (50.91%), Query Frame = 2

Query:  212 FQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            FQ+V+HG+ +  + ++ +    FF L  EEK +Y    G   GF  + V+  +  +DW +  +  + P++ R    +PK P   R T EVYS +L  L   L+  + E +
Sbjct:   71 FQVVDHGIDSGLISEMTRLSREFFALPAEEKLEYDTTGGKRGGFTISTVLQGDDAMDWREFVTYFSYPINARDYSRWPKKPEGWRSTTEVYSEKLMVLGAKLLEVLSEAM 180

HSP 2 Score: 32.3426 bits (72), Expect = 2.761e-13
Identity = 14/32 (43.75%), Postives = 21/32 (65.62%), Query Frame = 3

Query:  609 PPCPQPEKVMGLSPHSDSAALTILVTLNEVGG 704
            P CPQP+  +G+  H+D   +TIL+  + VGG
Sbjct:  204 PTCPQPDLTLGVRRHTDPGTITILLQ-DMVGG 234

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: LDOX_PERFR (Leucoanthocyanidin dioxygenase OS=Perilla frutescens GN=ANS PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.660e-13
Identity = 30/104 (28.85%), Postives = 53/104 (50.96%), Query Frame = 2

Query:  218 LVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLIS 523
            L+NHG+    ++++K   + FF L +EEK+ Y   Q  G+++G+G     +   +L+W D F     P H     ++P  PP        Y+ +L +LA  ++S
Sbjct:   88 LINHGIPEELIDRVKAAGKEFFELPVEEKEAYANDQAAGNVQGYGSKLANNASGQLEWEDYFFHCVYPEHKTDLSIWPTKPPDYIPATSEYAKQLRALATKILS 191

HSP 2 Score: 35.8094 bits (81), Expect = 4.660e-13
Identity = 15/37 (40.54%), Postives = 24/37 (64.86%), Query Frame = 3

Query:  573 ENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILV 683
            E+ +  M++N  P CPQPE  +G   H+D +ALT ++
Sbjct:  212 EDLIVQMKINFYPKCPQPELALGWEAHTDVSALTFIL 248

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS1_ARATH (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.671e-13
Identity = 34/112 (30.36%), Postives = 52/112 (46.43%), Query Frame = 2

Query:  212 FQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            FQ+VNHG+ T  + +L+     FF L   EK+     +   D+EG+G       E K  W D       P        +PK PP  RE  E Y++ ++ L+E L+  + + L
Sbjct:   70 FQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDGL 181

HSP 2 Score: 38.891 bits (89), Expect = 4.671e-13
Identity = 17/38 (44.74%), Postives = 27/38 (71.05%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGG 704
            M++N  PPCP+P+  +G+  H+D + +T+LV  NEV G
Sbjct:  201 MKINYYPPCPRPDLALGVPAHTDLSGITLLVP-NEVPG 237

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS_EUSGR (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma grandiflorum GN=FLS PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.023e-12
Identity = 34/110 (30.91%), Postives = 52/110 (47.27%), Query Frame = 2

Query:  194 AKNGVFFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGD--MEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENL 517
            +K    FQ+VNHG+    + KL++  + FF L  EEK+   +  G   +EG+G       + K  W D       P        +PK PP+ RE  E Y+  L+ + +NL
Sbjct:   64 SKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQKEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNL 173

HSP 2 Score: 38.5058 bits (88), Expect = 1.023e-12
Identity = 21/50 (42.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            M++N  PPCP+P+  +G++ H+D +A+T+LV  NEV G   L+  + G W
Sbjct:  201 MKINYYPPCPRPDLALGVA-HTDMSAITVLVP-NEVPG---LQVYKDGHW 245

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.325e-12
Identity = 33/116 (28.45%), Postives = 55/116 (47.41%), Query Frame = 2

Query:  209 FFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVH--LRKPHLFPKLPPSLRETLEVYS---MELESLAENLISKMGEVL 541
            FFQ+VNHG+    L    + V+ FF + + EK++  + PG+  G+  +F      KL W +  S  + P    L   ++   L    R   EVY+    E+  L+  ++  +GE L
Sbjct:   71 FFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESL 186

HSP 2 Score: 41.9726 bits (97), Expect = 1.325e-12
Identity = 25/59 (42.37%), Postives = 31/59 (52.54%), Query Frame = 3

Query:  564 RIFENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            R FE     MR+N  PPC +P + +G  PH D  +LTIL   N VGG   L+    G W
Sbjct:  195 RFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDN-VGG---LQVHTEGRW 249

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.721e-12
Identity = 33/116 (28.45%), Postives = 55/116 (47.41%), Query Frame = 2

Query:  209 FFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVH--LRKPHLFPKLPPSLRETLEVYS---MELESLAENLISKMGEVL 541
            FFQ+VNHG+    L    + V+ FF + + EK++  + PG+  G+  +F      KL W +  S  + P    L   ++   L    R   EVY+    E+  L+  ++  +GE L
Sbjct:   71 FFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESL 186

HSP 2 Score: 41.5874 bits (96), Expect = 1.721e-12
Identity = 25/59 (42.37%), Postives = 31/59 (52.54%), Query Frame = 3

Query:  564 RIFENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            R FE     MR+N  PPC +P + +G  PH D  +LTIL   N VGG   L+    G W
Sbjct:  195 RFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDN-VGG---LQVHTEGRW 249

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS_MALDO (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica GN=FLS PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.241e-12
Identity = 35/117 (29.91%), Postives = 54/117 (46.15%), Query Frame = 2

Query:  185 TSHAKNGVFFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHP--GDMEGFG----QAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENL 517
            T  + N   +Q+VNH + +  + KL+   + FF L  EEK+ Y + P    +EG+G    +     +  K  W D       P  +     +PK PPS RE  E Y+  L ++ E L
Sbjct:   59 TEASSNWGMYQIVNHDIPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDNLFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKL 175

HSP 2 Score: 38.5058 bits (88), Expect = 2.241e-12
Identity = 19/50 (38.00%), Postives = 32/50 (64.00%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            +++N  PPCP+P+  +G+  H+D + +TILV  N+V G   L+  + G W
Sbjct:  203 LKINYYPPCPRPDLALGVVAHTDMSTVTILVP-NDVQG---LQACKDGRW 248

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS_PETHY (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida GN=FL PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.907e-12
Identity = 34/115 (29.57%), Postives = 53/115 (46.09%), Query Frame = 2

Query:  194 AKNGVFFQLVNHGMSTAFLEKLKKEVEGFF-NLSMEEKKKYWQHPG--DMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKM 529
            +K    FQL+NHG+    +  L+K  + FF ++  EEK+   + PG  D+EG+G +     E K  W D       P        +PK PPS RE  E Y   +  + + +   +
Sbjct:   76 SKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSLQKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSL 190

HSP 2 Score: 38.891 bits (89), Expect = 2.907e-12
Identity = 20/50 (40.00%), Postives = 32/50 (64.00%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            +++N  PPCP+P+  +G+  H+D + +TILV  NEV G   L+  + G W
Sbjct:  214 LKINYYPPCPRPDLALGVVAHTDMSYITILVP-NEVQG---LQVFKDGHW 259

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: ACCO2_MALDO (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica GN=ACO2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 2.912e-12
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 2

Query:  197 KNGVFFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            +N  FF+LVNHG+ST  L+ ++K  +  +  +ME++ K       +E      V SE   LDW   F +  LP         P L    R+T++ +++ELE LAE L+  + E L
Sbjct:   29 ENWGFFELVNHGISTELLDTVEKMNKDHYKKTMEQRFKEMVAAKGLEA-----VQSEIHYLDWESTFFLRHLP--SSNISEIPDLEEDYRKTMKEFAVELEKLAEKLLDLLCENL 136

HSP 2 Score: 37.3502 bits (85), Expect = 2.912e-12
Identity = 15/49 (30.61%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  594 RMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            ++++ PPCP+P+ + GL  H+D+  + +L   ++V G   L+ ++ G W
Sbjct:  158 KVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSG---LQLLKDGEW 203

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS_SOLTU (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 8.261e-12
Identity = 35/115 (30.43%), Postives = 54/115 (46.96%), Query Frame = 2

Query:  194 AKNGVFFQLVNHGMSTAFLEKLKKEVEGFF-NLSMEEKKKYWQHPG--DMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKM 529
            +K    FQ++NHG+    +E L+K  + FF  +  EEK+   + PG   +EG+G +     E K  W D       P        +PK PPS RE  E Y+  L  +A+ +   +
Sbjct:   80 SKEWGIFQVINHGIPDEVIENLQKVGKEFFEEVPQEEKELIAKKPGAQSLEGYGTSLQKEIEGKKGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKWLRKVADGIFRSL 194

HSP 2 Score: 36.5798 bits (83), Expect = 8.261e-12
Identity = 17/42 (40.48%), Postives = 29/42 (69.05%), Query Frame = 3

Query:  573 ENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEV 698
            E+ V  +++N  PPCP+P+  +G+  H+D + +T+LV  NEV
Sbjct:  212 EDIVYMLKINYYPPCPRPDLALGVVAHTDMSYITLLVP-NEV 252

The following BLAST results are available for this feature:

BLAST of EY720682 vs. ExPASy Swiss-Prot

BLAST of EY720682 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29


Match Name	E-value	Identity	Description
FNSI_PETCR	2.761e-13	30.00	Flavone synthase OS=Petroselinum crispum GN=FNSI P...	[more]
LDOX_PERFR	4.660e-13	28.85	Leucoanthocyanidin dioxygenase OS=Perilla frutesce...	[more]
FLS1_ARATH	4.671e-13	30.36	Flavonol synthase/flavanone 3-hydroxylase OS=Arabi...	[more]
FLS_EUSGR	1.023e-12	30.91	Flavonol synthase/flavanone 3-hydroxylase OS=Eusto...	[more]
GAO1B_WHEAT	1.325e-12	28.45	Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN...	[more]
GAO1A_WHEAT	1.721e-12	28.45	Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN...	[more]
FLS_MALDO	2.241e-12	29.91	Flavonol synthase/flavanone 3-hydroxylase OS=Malus...	[more]
FLS_PETHY	2.907e-12	29.57	Flavonol synthase/flavanone 3-hydroxylase OS=Petun...	[more]
ACCO2_MALDO	2.912e-12	33.04	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Mal...	[more]
FLS_SOLTU	8.261e-12	30.43	Flavonol synthase/flavanone 3-hydroxylase OS=Solan...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-012-H09-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720682 ID=EY720682; Name=EY720682; organism=Citrus sinensis; type=EST; length=774bp
GCGACCACGCGTCCGGTTCAAGAGTTGGTCAAGAATCCTGCGTTAGTTGT
CCCCGACAGATACATACGTCCAGATCAAGATTCACCACTCATTTCTGATG
ATACTTTGATTTCACAGATACCAGTTATTGACATGCAAAGCTTACTTTCT
GAAGAATCAATGGATTCTGAGCTAGCCAAGCTCGACTTCGCATGCAAAGA
ATGGGGTTTTTTTCCAGCTGGTAAATCATGGAATGAGCACAGCATTTCTA
GAGAAATTAAAGAAAGAGGTTGAGGGATTCTTCAATCTCAGCATGGAAGA
AAAGAAAAAATATTGGCAGCATCCAGGAGATATGGAAGGCTTTGGACAAG
CCTTTGTTGTATCTGAGGAACAAAAGCTTGATTGGGCTGACCTTTTCTCC
ATGCTGACTCTTCCAGTTCATCTGAGGAAGCCACACTTATTTCCTAAGCT
CCCTCCTTCCCTCAGAGAGACCTTGGAAGTGTACTCAATGGAATTAGAAT
CCCTAGCCGAGAACCTAATATCGAAAATGGGAGAAGTGTTAATATCAGAG
ATGAAGAAATGAGAGAATCTTTGAAAATGGAGTGCGAAGCATGAGGATGA
ATGATTAGCCTCCATGTCCTCAGCCCGAGAAGGTGATGGGCCTGTCTCCT
CATTCTGATTCAGCTGCACTTACCATTCTGGTGACACTTAATGAAGTTGG
AGGGTCCTCCCGGTTAAAAAGGATGGAAAGGGGATCCTGGTGAGCGGGTC
GCGGATGGTTTCATTGGCTCCATT