EY665325 | Citrus Genome Database

Overview


Name	EY665325
Unique Name	EY665325
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	968

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433E_HUMAN (14-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE=1 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.221e-45
Identity = 84/115 (73.04%), Postives = 98/115 (85.22%), Query Frame = 2

Query:    8 ERENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            +RE+ +Y AKLAEQAERYDEMV++MK VA +DVELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE KG E   K I+EYRQ VE+EL  IC DI+ V+D+HLIP+A+ GES
Sbjct:    3 DREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGES 117

HSP 2 Score: 36.5798 bits (83), Expect = 1.221e-45
Identity = 31/105 (29.52%), Postives = 39/105 (37.14%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            KGDY+RYLAEF                      +    +  P  PI+ GLALNF   YYE    P +     K  F     + D   +   + S  I   LR  L
Sbjct:  125 KGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAM-TELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNL 228

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433E_CHICK (14-3-3 protein epsilon OS=Gallus gallus GN=YWHAE PE=1 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.221e-45
Identity = 84/115 (73.04%), Postives = 98/115 (85.22%), Query Frame = 2

Query:    8 ERENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            +RE+ +Y AKLAEQAERYDEMV++MK VA +DVELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE KG E   K I+EYRQ VE+EL  IC DI+ V+D+HLIP+A+ GES
Sbjct:    3 DREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGES 117

HSP 2 Score: 36.5798 bits (83), Expect = 1.221e-45
Identity = 31/105 (29.52%), Postives = 39/105 (37.14%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            KGDY+RYLAEF                      +    +  P  PI+ GLALNF   YYE    P +     K  F     + D   +   + S  I   LR  L
Sbjct:  125 KGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAM-TELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNL 228

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433E_BOVIN (14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.221e-45
Identity = 84/115 (73.04%), Postives = 98/115 (85.22%), Query Frame = 2

Query:    8 ERENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            +RE+ +Y AKLAEQAERYDEMV++MK VA +DVELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE KG E   K I+EYRQ VE+EL  IC DI+ V+D+HLIP+A+ GES
Sbjct:    3 DREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGES 117

HSP 2 Score: 36.5798 bits (83), Expect = 1.221e-45
Identity = 31/105 (29.52%), Postives = 39/105 (37.14%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            KGDY+RYLAEF                      +    +  P  PI+ GLALNF   YYE    P +     K  F     + D   +   + S  I   LR  L
Sbjct:  125 KGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAM-TELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNL 228

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433_TRIHA (14-3-3 protein homolog OS=Trichoderma harzianum PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.702e-45
Identity = 80/113 (70.80%), Postives = 97/113 (85.84%), Query Frame = 2

Query:   14 ENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            E+ +Y+AKLAEQAERY+EMV+ MK VA  D +LTVEERNLLSV YKNVIGARRASWRI++SIEQKEE+KGN      IKEYRQK+E+EL+ IC+DI+ V+D+HLIPSA +GES
Sbjct:    4 EDAVYLAKLAEQAERYEEMVENMKIVASEDRDLTVEERNLLSVAYKNVIGARRASWRIVTSIEQKEESKGNSSQVTLIKEYRQKIENELAKICDDILEVLDQHLIPSAKSGES 116

HSP 2 Score: 40.817 bits (94), Expect = 2.702e-45
Identity = 33/105 (31.43%), Postives = 41/105 (39.05%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            KGDY+RYLAEF                      + Q  +  P  PI+ GLALNF   YYE    P +     KQ F     + D   +   + S  I   LR  L
Sbjct:  124 KGDYHRYLAEFAIGDRRKDSADKSLEAYKAATEVAQ-TELPPTHPIRLGLALNFSVFYYEILNAPDQACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNL 227

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14333_ORYSJ (14-3-3-like protein GF14-C OS=Oryza sativa subsp. japonica GN=GF14C PE=1 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 1.324e-44
Identity = 81/121 (66.94%), Postives = 99/121 (81.82%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAK-LDVE-LTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ VAK +DVE LTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE +GNE +   IKEYR K+E+ELS IC+ I+ ++D HL+PS++A ES    L
Sbjct:    3 REENVYMAKLAEQAERYEEMVEYMEKVAKTVDVEELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEEGRGNEEHVTLIKEYRGKIEAELSKICDGILKLLDSHLVPSSTAAESKVFYL 123

HSP 2 Score: 45.8246 bits (107), Expect = 1.324e-44
Identity = 31/76 (40.79%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   AD AP  PI+ GLALNF   YYE    P K     KQ F
Sbjct:  126 KGDYHRYLAEFKTGAERKEAAESTMVAYKAAQDIAL-ADLAPTHPIRLGLALNFSVFYYEILNSPDKACNLAKQAF 200

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14333_ARATH (14-3-3-like protein GF14 psi OS=Arabidopsis thaliana GN=GRF3 PE=1 SV=2)

HSP 1 Score: 153.68 bits (387), Expect = 2.930e-44
Identity = 83/121 (68.60%), Postives = 99/121 (81.82%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAK-LDVE-LTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ VAK +DVE L+VEERNLLSV YKNVIGARRASWRI+SSIEQKEE+KGNE +   IK+YR K+ESELS IC+ I+ V++ HLIPSAS  ES    L
Sbjct:    4 REENVYMAKLAEQAERYEEMVEFMEKVAKTVDVEELSVEERNLLSVAYKNVIGARRASWRIISSIEQKEESKGNEDHVAIIKDYRGKIESELSKICDGILNVLEAHLIPSASPAESKVFYL 124

HSP 2 Score: 44.669 bits (104), Expect = 2.930e-44
Identity = 29/76 (38.16%), Postives = 35/76 (46.05%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   A+ AP  PI+ GLALNF   YYE    P +  +  KQ F
Sbjct:  127 KGDYHRYLAEFKAGAERKEAAESTLVAYKSASDIAT-AELAPTHPIRLGLALNFSVFYYEILNSPDRACSLAKQAF 201

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14331_ENTHI (14-3-3 protein 1 OS=Entamoeba histolytica PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 3.822e-44
Identity = 73/114 (64.04%), Postives = 94/114 (82.46%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            RE+ +Y AKLAEQ+ERYDEMV  MK VA+++ EL++EERNLLSV YKNVIGA+RASWRI+SS+EQKE+AKGN+ + + IK YR K+E ELS  C+D++ VI E+L+P AS  ES
Sbjct:    4 REDCVYTAKLAEQSERYDEMVQCMKQVAEMEAELSIEERNLLSVAYKNVIGAKRASWRIISSLEQKEQAKGNDKHVEIIKGYRAKIEKELSTCCDDVLKVIQENLLPKASTSES 117

HSP 2 Score: 44.669 bits (104), Expect = 3.822e-44
Identity = 34/105 (32.38%), Postives = 42/105 (40.00%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            +GDYYRY AEF                       +  A+ AP  PI+ GLALNF   Y+E      K     KQ F     K D  P+ + + S  I   LR  L
Sbjct:  125 EGDYYRYFAEFTV-DEKRKEVADKSLAAYTEATEISNAELAPTHPIRLGLALNFSVFYFEIMNDADKACQLAKQAFDDAIAKLDEVPENMYKDSTLIMQLLRDNL 228

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14337_ARATH (14-3-3-like protein GF14 nu OS=Arabidopsis thaliana GN=GRF7 PE=1 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 6.482e-44
Identity = 79/124 (63.71%), Postives = 99/124 (79.84%), Query Frame = 2

Query:    2 SKERENFLYVAKLAEQAERYDEMVDAMKNVAK-LDV-ELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            S  RE  +Y+AKLAEQAERY+EMV+ M+ VAK +D  ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GN+ +   IK+YR K+E+ELS IC+ I+ ++D HL+P+AS  ES    L
Sbjct:    2 SSSREENVYLAKLAEQAERYEEMVEFMEKVAKTVDTDELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDDHVSIIKDYRGKIETELSKICDGILNLLDSHLVPTASLAESKVFYL 125

HSP 2 Score: 45.8246 bits (107), Expect = 6.482e-44
Identity = 30/76 (39.47%), Postives = 35/76 (46.05%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   AD AP  PI+ GLALNF   YYE    P +  +  KQ F
Sbjct:  128 KGDYHRYLAEFKTGAERKEAAESTLVAYKSAQDIAL-ADLAPTHPIRLGLALNFSVFYYEILNSPDRACSLAKQAF 202

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14332_SOLLC (14-3-3 protein 2 OS=Solanum lycopersicum GN=TFT2 PE=3 SV=2)

HSP 1 Score: 154.066 bits (388), Expect = 1.101e-43
Identity = 81/121 (66.94%), Postives = 97/121 (80.17%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKL--DVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV  M+ V+      ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE + K IKEYR K+ESELSDIC+ I+ ++D +LIPSAS G+S    L
Sbjct:    3 REENVYMAKLAEQAERYEEMVQFMEKVSTSLGSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEEHVKCIKEYRSKIESELSDICDGILKLLDSNLIPSASNGDSKVFYL 123

HSP 2 Score: 42.3578 bits (98), Expect = 1.101e-43
Identity = 28/76 (36.84%), Postives = 33/76 (43.42%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +    + AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  126 KGDYHRYLAEFKTGAERKEAAESTLSAYKAAQDIAN-TELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 200

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433_MESCR (14-3-3-like protein OS=Mesembryanthemum crystallinum GN=GBF PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.434e-43
Identity = 79/121 (65.29%), Postives = 97/121 (80.17%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKLD--VELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ VAK+    EL+VEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   IKEYR K+E+ELS IC+ I+ +++ HLIPSAS  ES    L
Sbjct:    7 REENVYMAKLAEQAERYEEMVEFMEKVAKMTDTEELSVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVSTIKEYRGKIETELSKICDGILNLLESHLIPSASTAESKVFYL 127

HSP 2 Score: 43.1282 bits (100), Expect = 1.434e-43
Identity = 29/76 (38.16%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   A+ AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  130 KGDYHRYLAEFKTGAERKEAAENTLLAYKSAQDIAL-AELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 204

The following BLAST results are available for this feature:

BLAST of EY665325 vs. ExPASy Swiss-Prot

BLAST of EY665325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 142


Match Name	E-value	Identity	Description
1433E_HUMAN	1.221e-45	73.04	14-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE...	[more]
1433E_CHICK	1.221e-45	73.04	14-3-3 protein epsilon OS=Gallus gallus GN=YWHAE P...	[more]
1433E_BOVIN	1.221e-45	73.04	14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2...	[more]
1433_TRIHA	2.702e-45	70.80	14-3-3 protein homolog OS=Trichoderma harzianum PE...	[more]
14333_ORYSJ	1.324e-44	66.94	14-3-3-like protein GF14-C OS=Oryza sativa subsp. ...	[more]
14333_ARATH	2.930e-44	68.60	14-3-3-like protein GF14 psi OS=Arabidopsis thalia...	[more]
14331_ENTHI	3.822e-44	64.04	14-3-3 protein 1 OS=Entamoeba histolytica PE=3 SV=...	[more]
14337_ARATH	6.482e-44	63.71	14-3-3-like protein GF14 nu OS=Arabidopsis thalian...	[more]
14332_SOLLC	1.101e-43	66.94	14-3-3 protein 2 OS=Solanum lycopersicum GN=TFT2 P...	[more]
1433_MESCR	1.434e-43	65.29	14-3-3-like protein OS=Mesembryanthemum crystallin...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-086-F02-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665325 ID=EY665325; Name=EY665325; organism=Citrus sinensis; type=EST; length=968bp
CTCTAAAGAACGCGAAAACTTCCTCTACGTCGCCAAGCTCGCTGAACAAG
CCGAGCGCTACGACGAGATGGTGGATGCGATGAAAAATGTTGCGAAGCTT
GATGTGGAATTGACGGTTGAGGAGCGCAACTTGCTCTCTGTTGGCTACAA
GAATGTAATTGGTGCTCGAAGAGCATCATGGAGGATACTTTCGTCAATTG
AGCAAAAGGAAGAAGCGAAAGGGAATGAAGTGAATGCGAAGCGGATCAAG
GAGTATAGGCAAAAGGTTGAATCTGAATTGTCGGACATTTGCAATGATAT
CATGACTGTCATTGATGAGCATCTTATTCCTTCAGCAAGTGCTGGTGAAT
CCAACTGTGTTCTTCTATAAGATGAAAGGGAGATTATTATCGGTACCTTG
CTGAATTCAAGTTTTGGCGATAAAGAAGAAAGAGGCTGCTGCAAATTCAA
TGAAAGCGTATGAGACAGCTACCACTGCTGCAGAAGGCTGATTTTGCCCC
TAACCTTCCAATCCAATGGGGTTTGGCTCTGAATTTCCCTGGCCTTTATT
ATGAAAATCATGAAATTCCCCTAAAAGGGCTGGCCACTTAGAAACAAACC
TTTTGGGGAACCTTTCCAAAGCGGGACCCCCCACAGGGGATCCTACGAAA
AAGCCCCTTTATTTTCCCCTTTCTAAGGACAACCTTACCCTATGGATTCG
GAAATCCAAAAAAAGAAAAAACCCAAAAGGGAAAGGGATACGCCAAAATG
GGGGGGAATAAATCAAATGTCAAAAAAAAGAAGAAAATTTTTTTGGAGAG
GGGAAAAAGGGGAAATTTTTAACACAATTGGTGTACGAAGGAGGGCTTGT
GTTTGTGTGGGTTTATTCTATTAAAGCCCAATTTTGGGAGGAGAAATTTA
TTGAGGGGGGGGACAATGATGAAGGCGCCTGTCGCGCACCAGTTATTCAC
CAATCTGGAAGGGAGGGA