EY665325 | Citrus Genome Database

Overview


Name	EY665325
Unique Name	EY665325
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	968

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433_PEA (14-3-3-like protein OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 7.609e-42
Identity = 79/121 (65.29%), Postives = 98/121 (80.99%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNV-AKLDVE-LTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ V A  D E LTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   I++YR K+ESELS+IC+ I+ ++D  LIPSAS+G+S    L
Sbjct:    8 REENVYMAKLAEQAERYEEMVEFMEKVSANADSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVAVIRDYRSKIESELSNICDGILKLLDTRLIPSASSGDSKVFYL 128

HSP 2 Score: 41.9726 bits (97), Expect = 7.609e-42
Identity = 28/76 (36.84%), Postives = 33/76 (43.42%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   A+  P  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  131 KGDYHRYLAEFKTGAERKEAAESTLTGYKSAQDIAN-AELPPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 205

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14336_SOLLC (14-3-3 protein 6 OS=Solanum lycopersicum GN=TFT6 PE=2 SV=2)

HSP 1 Score: 146.362 bits (368), Expect = 7.610e-42
Identity = 80/122 (65.57%), Postives = 95/122 (77.87%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAM-KNVAKLD--VELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M K VA  D   ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   IKEYR K+ESEL+ ICN I+ ++D  LI SA+ G+S    L
Sbjct:    5 REENVYMAKLAEQAERYEEMVEFMEKVVAAADGAEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVASIKEYRSKIESELTSICNGILKLLDSKLIGSAATGDSKVFYL 126

HSP 2 Score: 43.8986 bits (102), Expect = 7.610e-42
Identity = 29/76 (38.16%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   A+ AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  129 KGDYHRYLAEFKTGAERKEAAENTLSAYKAAQDIAN-AELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 203

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14335_SOLTU (14-3-3-like protein 16R OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 7.610e-42
Identity = 80/122 (65.57%), Postives = 95/122 (77.87%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAM-KNVAKLD--VELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M K VA  D   ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   IKEYR K+ESEL+ ICN I+ ++D  LI SA+ G+S    L
Sbjct:    5 REENVYMAKLAEQAERYEEMVEFMEKVVAAADGAEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVASIKEYRSKIESELTSICNGILKLLDSKLIGSAATGDSKVFYL 126

HSP 2 Score: 43.8986 bits (102), Expect = 7.610e-42
Identity = 29/76 (38.16%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   A+ AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  129 KGDYHRYLAEFKTGAERKEAAENTLSAYKAAQDIAN-AELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 203

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433A_SOYBN (14-3-3-like protein A OS=Glycine max GN=GF14A PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.610e-42
Identity = 77/121 (63.64%), Postives = 100/121 (82.64%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAK-LDVE-LTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLA++AERY+EMV+ M+ VAK ++VE LTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   IKEYR K+E+ELS IC+ I+ +++ +LIPSA++ ES    L
Sbjct:    6 REENVYMAKLADEAERYEEMVEFMEKVAKTVEVEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVAIIKEYRGKIEAELSKICDGILNLLESNLIPSAASPESKVFYL 126

HSP 2 Score: 44.669 bits (104), Expect = 7.610e-42
Identity = 30/76 (39.47%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +   AD AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  129 KGDYHRYLAEFKTGAERKEAAESTLLAYKSAQDIAL-ADLAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 203

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433C_TOBAC (14-3-3-like protein C OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 9.915e-42
Identity = 76/121 (62.81%), Postives = 96/121 (79.34%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKL--DVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ V+      ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   I+EYR K+E+ELS IC+ I+ ++D  LIPSA++G+S    L
Sbjct:    8 REENVYMAKLAEQAERYEEMVEFMEKVSNSLGSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEEHVNSIREYRSKIENELSKICDGILKLLDAKLIPSAASGDSKVFYL 128

HSP 2 Score: 41.9726 bits (97), Expect = 9.915e-42
Identity = 28/76 (36.84%), Postives = 33/76 (43.42%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDY+RYLAEFK                     +    + AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  131 KGDYHRYLAEFKTGAERKEAAESTLTAYKAAQDIAT-TELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 205

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433D_TOBAC (14-3-3-like protein D OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.293e-41
Identity = 75/121 (61.98%), Postives = 93/121 (76.86%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKLDV--ELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  LY+AKLAEQAERY+EMV  M  +  +    ELTVEERNLLSV YKNVIG+ RA+WRI+SSIEQKEE + NE +   +K+YR KVESELSD+C  I+ ++D++LIPSA+AGES    L
Sbjct:    9 REQCLYLAKLAEQAERYEEMVKFMDRLVAVSASSELTVEERNLLSVAYKNVIGSLRAAWRIVSSIEQKEEGRKNEEHVVLVKDYRSKVESELSDVCAGILKILDQYLIPSAAAGESKVFYL 129

HSP 2 Score: 44.2838 bits (103), Expect = 1.293e-41
Identity = 29/76 (38.16%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDYYRYLAEFK                     +   A+ AP  PI+ GLALN+   YYE      K  +  KQ F
Sbjct:  132 KGDYYRYLAEFKVGNERKEAAEDTMLAYKAAQDIAL-AELAPTHPIRLGLALNYSVFYYEILNASEKACSMAKQAF 206

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14335_ARATH (14-3-3-like protein GF14 upsilon OS=Arabidopsis thaliana GN=GRF5 PE=1 SV=2)

HSP 1 Score: 146.362 bits (368), Expect = 1.683e-41
Identity = 76/121 (62.81%), Postives = 95/121 (78.51%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKL--DVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ VAK     ELTVEERNLLSV YKNVIGARRASWRI+SSIEQKE+++GN  +   IK+YR K+E+ELS IC+ I+ +++ HLIP+AS  ES    L
Sbjct:    7 REENVYLAKLAEQAERYEEMVEFMEKVAKTVETEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEDSRGNSDHVSIIKDYRGKIETELSKICDGILNLLEAHLIPAASLAESKVFYL 127

HSP 2 Score: 42.743 bits (99), Expect = 1.683e-41
Identity = 26/60 (43.33%), Postives = 29/60 (48.33%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYE 555
            KGDY+RYLAEFK                     +   AD AP  PI+ GLALNF   YYE
Sbjct:  130 KGDYHRYLAEFKTGAERKEAAESTLVAYKSAQDIAL-ADLAPTHPIRLGLALNFSVFYYE 188

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 1433A_TOBAC (14-3-3-like protein A OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 1.684e-41
Identity = 75/120 (62.50%), Postives = 93/120 (77.50%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKNVAKL-DVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  +Y+AKLAEQAERY+EMV+ M+ V    D ELT+EERNLLSV YKNVIGARRASWRI+SSIEQKEE++GNE +   IK YR K+ESEL+ ICN I+ ++D  LI +A+ G+S    L
Sbjct:    5 REENVYLAKLAEQAERYEEMVEFMEKVVGAGDDELTIEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVASIKTYRSKIESELTSICNGILKLLDSKLIGTAATGDSKVFYL 124

HSP 2 Score: 44.2838 bits (103), Expect = 1.684e-41
Identity = 29/76 (38.16%), Postives = 33/76 (43.42%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDYYRYLAEFK                     +    + AP  PI+ GLALNF   YYE    P +     KQ F
Sbjct:  127 KGDYYRYLAEFKTGAERKEAAENTLSAYKSAQDIAN-GELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAF 201

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: 14331_SOLLC (14-3-3 protein 1 OS=Solanum lycopersicum GN=TFT1 PE=3 SV=2)

HSP 1 Score: 143.665 bits (361), Expect = 1.685e-41
Identity = 75/121 (61.98%), Postives = 92/121 (76.03%), Query Frame = 2

Query:   11 RENFLYVAKLAEQAERYDEMVDAMKN--VAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAKGNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGESNCVLL 367
            RE  LY+AKLAEQAERY+EMV  M    +     ELTVEERNLLSV YKNVIG+ RA+WRI+SSIEQKEE + N+ +   +K+YR KVESELSD+C  I+ ++D++LIPSASAGES    L
Sbjct:    9 REQCLYLAKLAEQAERYEEMVKFMDKLVIGSGSSELTVEERNLLSVAYKNVIGSLRAAWRIVSSIEQKEEGRKNDEHVVLVKDYRSKVESELSDVCAGILKILDQYLIPSASAGESKVFYL 129

HSP 2 Score: 45.4394 bits (106), Expect = 1.685e-41
Identity = 30/76 (39.47%), Postives = 34/76 (44.74%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTF 603
            KGDYYRYLAEFK                     +   A+ AP  PI+ GLALNF   YYE      K  +  KQ F
Sbjct:  132 KGDYYRYLAEFKVGNERKEAAEDTMLAYKAAQDIAV-AELAPTHPIRLGLALNFSVFYYEILNASEKACSMAKQAF 206

[Close]

BLAST of EY665325 vs. ExPASy Swiss-Prot
Match: BMH2_YEAST (Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 PE=1 SV=3)

HSP 1 Score: 147.517 bits (371), Expect = 2.856e-41
Identity = 77/118 (65.25%), Postives = 97/118 (82.20%), Query Frame = 2

Query:    2 SKERENFLYVAKLAEQAERYDEMVDAMKNVAKLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKEEAK-GNEVNAKRIKEYRQKVESELSDICNDIMTVIDEHLIPSASAGES 352
            S+ RE+ +Y+AKLAEQAERY+EMV+ MK VA    EL+VEERNLLSV YKNVIGARRASWRI+SSIEQKEE+K  +E   + I+ YR K+E+EL+ I +DI++V+D HLIPSA+ GES
Sbjct:    2 SQTREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGES 119

HSP 2 Score: 40.817 bits (94), Expect = 2.856e-41
Identity = 33/105 (31.43%), Postives = 40/105 (38.10%), Query Frame = 1

Query:  376 KGDYYRYLAEFKFWXXXXXXXXXXXXXXXXXXPLLQKADFAPNLPIQWGLALNFPGLYYENHEIPLKGLAT*KQTFWGTFPKRDP-PQGILRKSPFIFPFLRTTL 687
            KGDY+RYLAEF                      +    +  P  PI+ GLALNF   YYE    P K     KQ F     + D   +   + S  I   LR  L
Sbjct:  127 KGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIAT-TELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNL 230

The following BLAST results are available for this feature:

BLAST of EY665325 vs. ExPASy Swiss-Prot

BLAST of EY665325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 142


Match Name	E-value	Identity	Description
1433_PEA	7.609e-42	65.29	14-3-3-like protein OS=Pisum sativum PE=2 SV=1	[more]
14336_SOLLC	7.610e-42	65.57	14-3-3 protein 6 OS=Solanum lycopersicum GN=TFT6 P...	[more]
14335_SOLTU	7.610e-42	65.57	14-3-3-like protein 16R OS=Solanum tuberosum PE=2 ...	[more]
1433A_SOYBN	7.610e-42	63.64	14-3-3-like protein A OS=Glycine max GN=GF14A PE=2...	[more]
1433C_TOBAC	9.915e-42	62.81	14-3-3-like protein C OS=Nicotiana tabacum PE=1 SV...	[more]
1433D_TOBAC	1.293e-41	61.98	14-3-3-like protein D OS=Nicotiana tabacum PE=2 SV...	[more]
14335_ARATH	1.683e-41	62.81	14-3-3-like protein GF14 upsilon OS=Arabidopsis th...	[more]
1433A_TOBAC	1.684e-41	62.50	14-3-3-like protein A OS=Nicotiana tabacum PE=2 SV...	[more]
14331_SOLLC	1.685e-41	61.98	14-3-3 protein 1 OS=Solanum lycopersicum GN=TFT1 P...	[more]
BMH2_YEAST	2.856e-41	65.25	Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 P...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-101-086-F02-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665325 ID=EY665325; Name=EY665325; organism=Citrus sinensis; type=EST; length=968bp
CTCTAAAGAACGCGAAAACTTCCTCTACGTCGCCAAGCTCGCTGAACAAG
CCGAGCGCTACGACGAGATGGTGGATGCGATGAAAAATGTTGCGAAGCTT
GATGTGGAATTGACGGTTGAGGAGCGCAACTTGCTCTCTGTTGGCTACAA
GAATGTAATTGGTGCTCGAAGAGCATCATGGAGGATACTTTCGTCAATTG
AGCAAAAGGAAGAAGCGAAAGGGAATGAAGTGAATGCGAAGCGGATCAAG
GAGTATAGGCAAAAGGTTGAATCTGAATTGTCGGACATTTGCAATGATAT
CATGACTGTCATTGATGAGCATCTTATTCCTTCAGCAAGTGCTGGTGAAT
CCAACTGTGTTCTTCTATAAGATGAAAGGGAGATTATTATCGGTACCTTG
CTGAATTCAAGTTTTGGCGATAAAGAAGAAAGAGGCTGCTGCAAATTCAA
TGAAAGCGTATGAGACAGCTACCACTGCTGCAGAAGGCTGATTTTGCCCC
TAACCTTCCAATCCAATGGGGTTTGGCTCTGAATTTCCCTGGCCTTTATT
ATGAAAATCATGAAATTCCCCTAAAAGGGCTGGCCACTTAGAAACAAACC
TTTTGGGGAACCTTTCCAAAGCGGGACCCCCCACAGGGGATCCTACGAAA
AAGCCCCTTTATTTTCCCCTTTCTAAGGACAACCTTACCCTATGGATTCG
GAAATCCAAAAAAAGAAAAAACCCAAAAGGGAAAGGGATACGCCAAAATG
GGGGGGAATAAATCAAATGTCAAAAAAAAGAAGAAAATTTTTTTGGAGAG
GGGAAAAAGGGGAAATTTTTAACACAATTGGTGTACGAAGGAGGGCTTGT
GTTTGTGTGGGTTTATTCTATTAAAGCCCAATTTTGGGAGGAGAAATTTA
TTGAGGGGGGGGACAATGATGAAGGCGCCTGTCGCGCACCAGTTATTCAC
CAATCTGGAAGGGAGGGA