CV884476

Overview
NameCV884476
Unique NameCV884476
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length854
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Library UCRCS04-UCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECOL5 (NAD-dependent malic enzyme OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.491e-31
Identity = 80/234 (34.19%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +GL+ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWHAEYRDYR 561
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECOK1 (NAD-dependent malic enzyme OS=Escherichia coli O1:K1 / APEC GN=sfcA PE=3 SV=2)

HSP 1 Score: 135.576 bits (340), Expect = 3.491e-31
Identity = 80/234 (34.19%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +GL+ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWHAEYRDYR 561
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECO7I (NAD-dependent malic enzyme OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.491e-31
Identity = 80/234 (34.19%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +GL+ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWHAEYRDYR 561
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECO45 (NAD-dependent malic enzyme OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.491e-31
Identity = 80/234 (34.19%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +GL+ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWHAEYRDYR 561
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECO27 (NAD-dependent malic enzyme OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.491e-31
Identity = 80/234 (34.19%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +GL+ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWHAEYRDYR 561
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_SHESR (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.560e-31
Identity = 83/234 (35.47%), Postives = 128/234 (54.70%), Query Frame = -2 
Query:  179 KICLVDSKGLIVSSRKDSLQHFKK---------PWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQN-LVKCAESCMYTPVYRNYR 850
            ++C+VD  GL++ +  + L   +K          W +  + ++ LLD V  +KPT+LIG SG    FT+E++ AM S  E+P+I  LSNPTS+ E T ++   W+ G+A+ A+GSPF+P   +G+ +   Q NN++IFPG GLG++ SGA  V D ML+A+S ALA+         G + P   +I              A E G++  LP     L++  E   + P YR Y+
Sbjct:  328 QVCMVDRWGLLLDNMPNLLPFQQKLAQKCADISHWNNFSDNIS-LLDVVNNVKPTVLIGVSGAPGLFTEEIVRAMHSHCERPIIFPLSNPTSRVEATPKDILHWTSGQALVATGSPFEPVVVDGETYEIAQCNNSFIFPGIGLGVLASGARHVSDAMLMASSRALAECSPLAINGSGPLLPKLEDIHSVSKHIAFAVGKVAIEQGLS--LPASDELLMQSIEDNFWKPEYRRYK 558
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_SHESM (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.560e-31
Identity = 83/234 (35.47%), Postives = 128/234 (54.70%), Query Frame = -2 
Query:  179 KICLVDSKGLIVSSRKDSLQHFKK---------PWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQN-LVKCAESCMYTPVYRNYR 850
            ++C+VD  GL++ +  + L   +K          W +  + ++ LLD V  +KPT+LIG SG    FT+E++ AM S  E+P+I  LSNPTS+ E T ++   W+ G+A+ A+GSPF+P   +G+ +   Q NN++IFPG GLG++ SGA  V D ML+A+S ALA+         G + P   +I              A E G++  LP     L++  E   + P YR Y+
Sbjct:  328 QVCMVDRWGLLLDNMPNLLPFQQKLAQKCADISHWNNFSDNIS-LLDVVNNVKPTVLIGVSGAPGLFTEEIVRAMHSHCERPIIFPLSNPTSRVEATPKDILHWTSGQALVATGSPFEPVVVDGETYEIAQCNNSFIFPGIGLGVLASGARHVSDAMLMASSRALAECSPLAINGSGPLLPKLEDIHSVSKHIAFAVGKVAIEQGLS--LPASDELLMQSIEDNFWKPEYRRYK 558
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB8 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS185) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.560e-31
Identity = 84/234 (35.90%), Postives = 128/234 (54.70%), Query Frame = -2 
Query:  179 KICLVDSKGLIVSSRKDSLQHFKK---------PWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLV-KCAESCMYTPVYRNYR 850
            ++C+VD  GL++ +  + L   +K          W++  + ++ LLD V   KPT+LIG SGV   FT+E+I AM S   +P+I  LSNPTS+ E T ++   W+ G+A+ A+GSPF+P   +G+ F   Q NN++IFPG GLG++ SGA  V D ML+A+S ALA+         G + P   +I              A E G+   LP    ++ +  E   ++P YR Y+
Sbjct:  328 QVCMVDRWGLLLDNMPNLLPFQQKLAQKCTNIQNWSNFSDNIS-LLDVVNNTKPTVLIGVSGVPGLFTEEIIRAMHSHCARPIIFPLSNPTSRVEATPKDILHWTSGQALVATGSPFEPVVVDGETFEIAQCNNSFIFPGIGLGVLASGARHVSDAMLMASSRALAECSPLAIDGSGPLLPKLEDIHAVSKHIAFAVGKVAVEQGLT--LPMSDEILQQSIEGNFWSPEYRRYK 558
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB2 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS223) GN=sfcA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.560e-31
Identity = 84/234 (35.90%), Postives = 128/234 (54.70%), Query Frame = -2 
Query:  179 KICLVDSKGLIVSSRKDSLQHFKK---------PWAHEHEPVNNLLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLV-KCAESCMYTPVYRNYR 850
            ++C+VD  GL++ +  + L   +K          W++  + ++ LLD V   KPT+LIG SGV   FT+E+I AM S   +P+I  LSNPTS+ E T ++   W+ G+A+ A+GSPF+P   +G+ F   Q NN++IFPG GLG++ SGA  V D ML+A+S ALA+         G + P   +I              A E G+   LP    ++ +  E   ++P YR Y+
Sbjct:  328 QVCMVDRWGLLLDNMPNLLPFQQKLAQKCTNIQNWSNFSDNIS-LLDVVNNTKPTVLIGVSGVPGLFTEEIIRAMHSHCARPIIFPLSNPTSRVEATPKDILHWTSGQALVATGSPFEPVVVDGETFEIAQCNNSFIFPGIGLGVLASGARHVSDAMLMASSRALAECSPLAIDGSGPLLPKLEDIHAVSKHIAFAVGKVAVEQGLT--LPMSDEILQQSIEGNFWSPEYRRYK 558
[Close]
BLAST of CV884476 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLI (NAD-dependent malic enzyme OS=Escherichia coli (strain K12) GN=sfcA PE=3 SV=4)

HSP 1 Score: 135.191 bits (339), Expect = 4.560e-31
Identity = 79/234 (33.76%), Postives = 130/234 (55.56%), Query Frame = -2 
Query:  179 KKICLVDSKGLIVSSRKDSLQHFKKPWAHEHEPVNN---------LLDAVKVIKPTILIGSSGVGRTFTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFEYNGKVFVPGQANNAYIFPGFGLGLVISGAIRVHDDMLLAASEALAKQVTEENFEKGLIYPPFSNIRXXXXXXXXXXXXXAYELGVATRLPRPQNLVKCAESCMYTPVYRNYR 853
            +K+ +VD  GL+ + +  +L  F+     + E +++         LLD V+ +KP ILIG SG    FT+E+I  M     +P+++ LSNPTS+ E T ++   W++G A+ A+GSPF+P  +  K++   Q NNA+IFPG GLG++ SGA R+ D+ML++ASE LA+        +G++ P   +I+             A + GVA +    + L +  +   +   YR+YR
Sbjct:  330 QKVFMVDRFGLL-TDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVK-TSAEALQQAIDDNFWQAEYRDYR 561
The following BLAST results are available for this feature:
BLAST of CV884476 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_ECOL53.491e-3134.19NAD-dependent malic enzyme OS=Escherichia coli O6:... [more]
MAO1_ECOK13.491e-3134.19NAD-dependent malic enzyme OS=Escherichia coli O1:... [more]
MAO1_ECO7I3.491e-3134.19NAD-dependent malic enzyme OS=Escherichia coli O7:... [more]
MAO1_ECO453.491e-3134.19NAD-dependent malic enzyme OS=Escherichia coli O45... [more]
MAO1_ECO273.491e-3134.19NAD-dependent malic enzyme OS=Escherichia coli O12... [more]
MAO1_SHESR4.560e-3135.47NAD-dependent malic enzyme OS=Shewanella sp. (stra... [more]
MAO1_SHESM4.560e-3135.47NAD-dependent malic enzyme OS=Shewanella sp. (stra... [more]
MAO1_SHEB84.560e-3135.90NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_SHEB24.560e-3135.90NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_ECOLI4.560e-3133.76NAD-dependent malic enzyme OS=Escherichia coli (st... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_2_003F10_T7 Ruby Orange Developing Flower cDNA Library UCRCS04-UCR Citrus sinensis cDNA clone UCRCS04_003_T7_F10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV884476 ID=CV884476; Name=CV884476; organism=Citrus sinensis; type=EST; length=854bp
TTTTTTTTTTTTTTTTTCACCATGTAGGCAGTGGTCCAACACCTTCATTT
GATAAGTACACATATACAGCAATACTTACACAAGTACAAAAGCCACCATA
ACACACCAAAACAAGTTATTTGACATTATTTTTGCTTAAAGACACTCAGC
GGAGACAGAAATTTTGGCAGAACATTTAACGATAGTTTCGGTAGACAGGG
GTATACATGCAACTTTCAGCGCACTTCACTAGATTCTGAGGTCGAGGGAG
ACGCGTAGCCACGCCTAGTTCATATGCCTTAGCAGCAACATTAGCAGCTA
TATTGGCTGAAATTTTTCTAATATTAGAAAATGGTGGGTAGATCAATCCC
TTCTCAAAGTTCTCTTCTGTCACTTGTTTTGCCAAGGCTTCCGAGGCTGC
CAGAAGCATGTCGTCATGTACCCGAATTGCTCCAGAGATGACCAAACCCA
AACCAAATCCAGGGAAGATGTAAGCATTGTTGGCCTGGCCAGGAACAAAA
ACTTTTCCATTGTATTCAAAAGGATCAAATGGACTTCCACTAGCGAAAAT
TGCACGACCCTTACTCCAAGTGTAAGCTTCTTCAGCTGTACATTCTGACT
GTGAGGTTGGGTTGGAGAGAGCCAGGATGAGAGGTTTCTCATTGAAGGAG
GCCATGGCCTCGATTACTTCTTTTGTAAAAGTTCTTCCAACTCCAGATGA
TCCAATCAAAATTGTTGGCTTGATTACCTTGACAGCATCTAAGAGATTGT
TGACAGGCTCATGTTCATGAGCCCAAGGCTTCTTGAAGTGTTGAAGTGAA
TCTTTCCGGGAGCTGACTATCAGTCCCTTTGAGTCTACAAGACATATCTT
CTTC
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