CV712161

Overview
NameCV712161
Unique NameCV712161
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length825
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3195 contig Csv1_Contig3195:92..915. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_MAGGR (ATP-dependent RNA helicase eIF4A OS=Magnaporthe grisea GN=TIF1 PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 2.833e-69
Identity = 125/184 (67.93%), Postives = 151/184 (82.07%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF   +LQ++D  + QCQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G  VVVGTPGRV DM++R+ L+ D +KMFVLDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   16 SNYDETVDSFDEMNLKSELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDTNVKQCQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDGMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQV 199

HSP 2 Score: 27.335 bits (59), Expect = 2.833e-69
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  201 LLSATMPQDVLEVTTKFM 218
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_CHICK (Eukaryotic initiation factor 4A-II OS=Gallus gallus GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.694e-69
Identity = 129/185 (69.73%), Postives = 152/185 (82.16%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+  L + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIDLKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLSTNIQV 210

HSP 2 Score: 28.1054 bits (61), Expect = 3.694e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPMDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASPFU (ATP-dependent RNA helicase eIF4A OS=Aspergillus fumigatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 4.817e-69
Identity = 125/184 (67.93%), Postives = 151/184 (82.07%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VRED + LQ G  VVVGTPGRV DM++R+ LR D +KMF+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   18 SNYDEITDSFDAMDLKPELLRGVYAYGFERPSAIQQRAILPIIKGNDVIAQAQSGTGKTATFSISALQKLDPNVKGCQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTNVREDMKALQEGPQVVVGTPGRVQDMIQRRVLRTDNLKMFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 201

HSP 2 Score: 27.335 bits (59), Expect = 4.817e-69
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  203 LLSATMPQDVLEVTTKFM 220
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_PANTR (Eukaryotic initiation factor 4A-I OS=Pan troglodytes GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.282e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 6.282e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_MOUSE (Eukaryotic initiation factor 4A-I OS=Mus musculus GN=Eif4a1 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.282e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 6.282e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_MACFA (Eukaryotic initiation factor 4A-I OS=Macaca fascicularis GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.282e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 6.282e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_HUMAN (Eukaryotic initiation factor 4A-I OS=Homo sapiens GN=EIF4A1 PE=1 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.282e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 6.282e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_BOVIN (Eukaryotic initiation factor 4A-I OS=Bos taurus GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.282e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 6.282e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_RABIT (Eukaryotic initiation factor 4A-I (Fragment) OS=Oryctolagus cuniculus GN=EIF4A1 PE=1 SV=2)

HSP 1 Score: 253.832 bits (647), Expect = 6.283e-69
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   17 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 201

HSP 2 Score: 28.4906 bits (62), Expect = 6.283e-69
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  203 LLSATMPSDVLEVTKKFM 220
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_NEUCR (ATP-dependent RNA helicase eIF4A OS=Neurospora crassa GN=tif-1 PE=3 SV=2)

HSP 1 Score: 254.603 bits (649), Expect = 8.194e-69
Identity = 125/184 (67.93%), Postives = 150/184 (81.52%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF   +LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G  VVVGTPGRV DM++R+ L+ D +KMFVLDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   17 SNYDETVDSFDEMNLKPELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPSLKACQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDSMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQV 200

HSP 2 Score: 27.335 bits (59), Expect = 8.194e-69
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  202 LLSATMPQDVLEVTTKFM 219
The following BLAST results are available for this feature:
BLAST of CV712161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_MAGGR2.833e-6967.93ATP-dependent RNA helicase eIF4A OS=Magnaporthe gr... [more]
IF4A2_CHICK3.694e-6969.73Eukaryotic initiation factor 4A-II OS=Gallus gallu... [more]
IF4A_ASPFU4.817e-6967.93ATP-dependent RNA helicase eIF4A OS=Aspergillus fu... [more]
IF4A1_PANTR6.282e-6969.73Eukaryotic initiation factor 4A-I OS=Pan troglodyt... [more]
IF4A1_MOUSE6.282e-6969.73Eukaryotic initiation factor 4A-I OS=Mus musculus ... [more]
IF4A1_MACFA6.282e-6969.73Eukaryotic initiation factor 4A-I OS=Macaca fascic... [more]
IF4A1_HUMAN6.282e-6969.73Eukaryotic initiation factor 4A-I OS=Homo sapiens ... [more]
IF4A1_BOVIN6.282e-6969.73Eukaryotic initiation factor 4A-I OS=Bos taurus GN... [more]
IF4A1_RABIT6.283e-6969.73Eukaryotic initiation factor 4A-I (Fragment) OS=Or... [more]
IF4A_NEUCR8.194e-6967.93ATP-dependent RNA helicase eIF4A OS=Neurospora cra... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_0001A04_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001A04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV712161 ID=CV712161; Name=CV712161; organism=Citrus sinensis; type=EST; length=825bp
AGTTTGGTAACCCTAGCAACTATTTTCTCTTAAATCAATCTTCTCCTTCT
CTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTTTAATCACCATC
ATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAGGATCTCAATTT
GATGCTCGTCAGTATGACACCAAGATGAATGAACTGCTCTCTACTGATGG
GCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAGTTTTGATGCAA
TGGGACTGCAGGAAAACCTTTTGAGGGGAATCTATGCCTATGGGTTTGAG
AAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACT
TGACGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAACAGCAACTTTCT
GCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCCAATGTCAGGCC
CTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGATTGAGAAAGTAAT
GAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATGCTTGTGTCGGTG
GGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTT
GTTGTTGGCACTCCTGGTCGTGTGTTTGACATGCTGCGCCGACAGTCTTT
ACGCCCTGATTACATTAAGATGTTTGTACTGGATGAGGCTGATGAAATGC
TTTCCCGTGGTTTTAAGGATCAGATCTATGATATTTTCCAGCTGCTTCCA
GCTAAAGTTCAGGTGGGGGGTTTTCTCTGCTACTATGCCTCCTGAGGCCT
TGGAGATTACAAGGAAGTTTATGAA
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