CV712161

Overview
NameCV712161
Unique NameCV712161
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length825
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3195 contig Csv1_Contig3195:92..915. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_NEOFI (ATP-dependent RNA helicase eIF4A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=tif1 PE=3 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 1.069e-68
Identity = 125/184 (67.93%), Postives = 151/184 (82.07%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFD+M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VRED + LQ G  VVVGTPGRV DM++R+ LR D +KMF+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   18 SNYDEITDSFDSMELKPELLRGIYAYGFERPSAIQQRAILPIIKGNDVIAQAQSGTGKTATFSISALQKLDPNVKGCQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTNVREDMKALQEGPQVVVGTPGRVQDMIQRRVLRTDNLKMFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 201

HSP 2 Score: 27.335 bits (59), Expect = 1.069e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  203 LLSATMPQDVLEVTTKFM 220
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_PHANO (ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria nodorum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 1.069e-68
Identity = 125/184 (67.93%), Postives = 150/184 (81.52%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE  DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  +  CQAL+LAPTRELAQQI+KV+ A+GD++ V  HAC+GGTSVR+D + LQ G  VVVGTPGRV DM++R+ L+ D++KMFVLDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   16 SNYDETTDSFDAMNLKAELLRGVYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISTLQKIDSNVKACQALILAPTRELAQQIQKVVVAIGDFMDVACHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRVLKTDHMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQV 199

HSP 2 Score: 27.335 bits (59), Expect = 1.069e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  201 LLSATMPQDVLEVTTKFM 218
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_RAT (Eukaryotic initiation factor 4A-II OS=Rattus norvegicus GN=Eif4a2 PE=1 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.393e-68
Identity = 128/185 (69.19%), Postives = 151/185 (81.62%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQV 210

HSP 2 Score: 28.4906 bits (62), Expect = 1.393e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPTDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_PONAB (Eukaryotic initiation factor 4A-II OS=Pongo abelii GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.393e-68
Identity = 128/185 (69.19%), Postives = 151/185 (81.62%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQV 210

HSP 2 Score: 28.4906 bits (62), Expect = 1.393e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPTDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_MOUSE (Eukaryotic initiation factor 4A-II OS=Mus musculus GN=Eif4a2 PE=2 SV=2)

HSP 1 Score: 252.677 bits (644), Expect = 1.393e-68
Identity = 128/185 (69.19%), Postives = 151/185 (81.62%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQV 210

HSP 2 Score: 28.4906 bits (62), Expect = 1.393e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPTDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_HUMAN (Eukaryotic initiation factor 4A-II OS=Homo sapiens GN=EIF4A2 PE=1 SV=2)

HSP 1 Score: 252.677 bits (644), Expect = 1.393e-68
Identity = 128/185 (69.19%), Postives = 151/185 (81.62%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQV 210

HSP 2 Score: 28.4906 bits (62), Expect = 1.393e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPTDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_BOVIN (Eukaryotic initiation factor 4A-II OS=Bos taurus GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.393e-68
Identity = 128/185 (69.19%), Postives = 151/185 (81.62%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADEMLSRGFKDQIY+IFQ L   +QV
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQV 210

HSP 2 Score: 28.4906 bits (62), Expect = 1.393e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  212 LLSATMPTDVLEVTKKFM 229
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASPOR (ATP-dependent RNA helicase eIF4A OS=Aspergillus oryzae GN=tif1 PE=3 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 1.816e-68
Identity = 126/199 (63.32%), Postives = 155/199 (77.89%), Query Frame = 1 
Query:  169 TKMNELLSTDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +++  L  + GQ   T+YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL+LAPTRELAQQI+KV+ A+GD++ +  HAC+GGT+VRED + L  G  VVVGTPGRV DM++R+ L+ D++K F+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   27 SRLTRLFQSTGQ-IETNYDEITDSFDAMELKPELLRGVYAYGFERPSAIQQRAIKPIIKGSDVIAQAQSGTGKTATFSISALQKLDPNVKGCQALILAPTRELAQQIQKVVVAIGDFMNITCHACIGGTAVREDMKALGEGPEVVVGTPGRVHDMIQRRVLKTDHLKQFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 224

HSP 2 Score: 27.335 bits (59), Expect = 1.816e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  226 LLSATMPQDVLEVTTKFM 243
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASPCL (ATP-dependent RNA helicase eIF4A OS=Aspergillus clavatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 1.817e-68
Identity = 124/184 (67.39%), Postives = 151/184 (82.07%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFD+M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VRED + LQ G  VVVGTPGRV DM++R+ LR D +K+F+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   18 SNYDEITDSFDSMDLKPELLRGIYAYGFERPSAIQQRAIMPIIKGSDVIAQAQSGTGKTATFSISALQKIDPNLKACQALILAPTRELAQQIQKVVVAIGDFMSLECHACIGGTNVREDMKALQDGPQVVVGTPGRVQDMIQRRVLRTDQMKLFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 201

HSP 2 Score: 27.335 bits (59), Expect = 1.817e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  203 LLSATMPQDVLEVTTKFM 220
[Close]
BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_CHAGB (ATP-dependent RNA helicase eIF4A OS=Chaetomium globosum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 2.370e-68
Identity = 123/184 (66.85%), Postives = 150/184 (81.52%), Query Frame = 1 
Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF   +LQ++D  +  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G  V+VGTPGRV DM++R+ L+ D +KMFVLDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   17 SNYDETVDSFDDMNLKSELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPTVKSCQALILAPTRELAQQIQKVVIAIGDFMNIECHACIGGTSVRDDMKALQDGPQVIVGTPGRVHDMIQRRILKTDQMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQV 200

HSP 2 Score: 27.335 bits (59), Expect = 2.370e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  202 LLSATMPQDVLEVTTKFM 219
The following BLAST results are available for this feature:
BLAST of CV712161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_NEOFI1.069e-6867.93ATP-dependent RNA helicase eIF4A OS=Neosartorya fi... [more]
IF4A_PHANO1.069e-6867.93ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria ... [more]
IF4A2_RAT1.393e-6869.19Eukaryotic initiation factor 4A-II OS=Rattus norve... [more]
IF4A2_PONAB1.393e-6869.19Eukaryotic initiation factor 4A-II OS=Pongo abelii... [more]
IF4A2_MOUSE1.393e-6869.19Eukaryotic initiation factor 4A-II OS=Mus musculus... [more]
IF4A2_HUMAN1.393e-6869.19Eukaryotic initiation factor 4A-II OS=Homo sapiens... [more]
IF4A2_BOVIN1.393e-6869.19Eukaryotic initiation factor 4A-II OS=Bos taurus G... [more]
IF4A_ASPOR1.816e-6863.32ATP-dependent RNA helicase eIF4A OS=Aspergillus or... [more]
IF4A_ASPCL1.817e-6867.39ATP-dependent RNA helicase eIF4A OS=Aspergillus cl... [more]
IF4A_CHAGB2.370e-6866.85ATP-dependent RNA helicase eIF4A OS=Chaetomium glo... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_0001A04_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001A04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV712161 ID=CV712161; Name=CV712161; organism=Citrus sinensis; type=EST; length=825bp
AGTTTGGTAACCCTAGCAACTATTTTCTCTTAAATCAATCTTCTCCTTCT
CTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTTTAATCACCATC
ATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAGGATCTCAATTT
GATGCTCGTCAGTATGACACCAAGATGAATGAACTGCTCTCTACTGATGG
GCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAGTTTTGATGCAA
TGGGACTGCAGGAAAACCTTTTGAGGGGAATCTATGCCTATGGGTTTGAG
AAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACT
TGACGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAACAGCAACTTTCT
GCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCCAATGTCAGGCC
CTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGATTGAGAAAGTAAT
GAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATGCTTGTGTCGGTG
GGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTT
GTTGTTGGCACTCCTGGTCGTGTGTTTGACATGCTGCGCCGACAGTCTTT
ACGCCCTGATTACATTAAGATGTTTGTACTGGATGAGGCTGATGAAATGC
TTTCCCGTGGTTTTAAGGATCAGATCTATGATATTTTCCAGCTGCTTCCA
GCTAAAGTTCAGGTGGGGGGTTTTCTCTGCTACTATGCCTCCTGAGGCCT
TGGAGATTACAAGGAAGTTTATGAA
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