CV712161 | Citrus Genome Database

Overview


Name	CV712161
Unique Name	CV712161
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	825

Libraries


Library Name	Type
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig3195	contig	Csv1_Contig3195:92..915.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_AJECN (ATP-dependent RNA helicase eIF4A OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=TIF1 PE=3 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 3.091e-68
Identity = 123/184 (66.85%), Postives = 149/184 (80.98%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GG SVRED + LQ G  VVVGTPGRV DM++R+ L+ D++KMFVLDEADEMLS GF +QIYDIFQ LP   QV
Sbjct:   18 SNYDEITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFMSIECHACIGGISVREDMKALQDGPQVVVGTPGRVHDMIQRRVLKTDHMKMFVLDEADEMLSHGFTEQIYDIFQFLPQSTQV 201

HSP 2 Score: 27.335 bits (59), Expect = 3.091e-68
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  203 LLSATMPQDVLEVTTKFM 220

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A2_MACFA (Eukaryotic initiation factor 4A-II OS=Macaca fascicularis GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 8.935e-68
Identity = 127/185 (68.65%), Postives = 150/185 (81.08%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEAD MLSRGFKDQIY+IFQ L   +QV
Sbjct:   27 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADGMLSRGFKDQIYEIFQKLNTSIQV 211

HSP 2 Score: 28.4906 bits (62), Expect = 8.935e-68
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  213 LLSATMPTDVLEVTKKFM 230

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_COCIM (ATP-dependent RNA helicase eIF4A OS=Coccidioides immitis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 1.165e-67
Identity = 124/184 (67.39%), Postives = 149/184 (80.98%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ V+ HAC+GGT+VRED + L  G  VVVGTPGRV DM++R+ L+ D +KMFVLDEADEMLSRGF +QIYDIFQ LP   QV
Sbjct:   18 SNYDEITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFMSVECHACIGGTNVREDIKALNDGPQVVVGTPGRVHDMIQRRVLKTDQMKMFVLDEADEMLSRGFTEQIYDIFQFLPQSTQV 201

HSP 2 Score: 26.1794 bits (56), Expect = 1.165e-67
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + L++T KFM
Sbjct:  203 LLSATMPQDVLDVTTKFM 220

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A1_PONAB (Eukaryotic initiation factor 4A-I OS=Pongo abelii GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 2.584e-67
Identity = 127/185 (68.65%), Postives = 147/185 (79.46%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++++E+ DS D M L E+LLRGIYAYGFEKPSAIQQR I+   KG DVI QAQSGTGKTATF   ILQQ++  L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++     H++VGTPGRVFDML R+ L P YIKMFVLDEADEMLSRGFKDQIYDIFQ L +  QV
Sbjct:   25 SNWNEIVDSLDDMNLSESLLRGIYAYGFEKPSAIQQRAILSCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQV 209

HSP 2 Score: 28.4906 bits (62), Expect = 2.584e-67
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T+KFM
Sbjct:  211 LLSATMPSDVLEVTKKFM 228

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_EMENI (ATP-dependent RNA helicase eIF4A OS=Emericella nidulans GN=tif1 PE=3 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 5.728e-67
Identity = 118/184 (64.13%), Postives = 150/184 (81.52%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++YDE+ DSFD+M L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL++APTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VR+D   L+ G  +VVGTPGR+ DM++R+ L+ D +KMF+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   41 SNYDEITDSFDSMELKPELLRGVYAYGFERPSAIQQRAILPIVKGNDVIAQAQSGTGKTATFSISALQKLDPNVKACQALIVAPTRELAQQIQKVVIAIGDFMNIQCHACIGGTAVRDDMNALREGPQIVVGTPGRIHDMIQRRVLKTDQMKMFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 224

HSP 2 Score: 27.335 bits (59), Expect = 5.728e-67
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  226 LLSATMPQDVLEVTTKFM 243

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASPNC (ATP-dependent RNA helicase eIF4A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN=tif1 PE=3 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 5.731e-67
Identity = 120/184 (65.22%), Postives = 148/184 (80.43%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            T+YDEV DSFD+M L+  LLRG+YAYGFE+PSAIQQR I P   G DVI QAQSGTGKTATF    LQ++D  L  CQAL++APTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VR+D   L+AG  VVVGTPGR+ DM+ R+ L+ D +K+F+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   18 TNYDEVTDSFDSMDLKPELLRGVYAYGFERPSAIQQRAIKPIIAGHDVIAQAQSGTGKTATFSISALQKIDQELKACQALIVAPTRELAQQIQKVVVAIGDFMNIECHACIGGTNVRDDMNALRAGPQVVVGTPGRIHDMIERRVLKTDQMKLFILDEADEMLSRGFTEQIYDIFQLLPQSTQV 201

HSP 2 Score: 27.335 bits (59), Expect = 5.731e-67
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  203 LLSATMPQDVLEVTTKFM 220

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_CAEEL (Eukaryotic initiation factor 4A OS=Caenorhabditis elegans GN=inf-1 PE=2 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 9.745e-67
Identity = 128/200 (64.00%), Postives = 155/200 (77.50%), Query Frame = 1

Query:  166 DTKMNELLSTDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            D  ++ ++  DG     +YD+V +SFD M L+E LLRGIY +GFEKPSAIQ+R IVP   G DVI QAQSGTGKTATF   ILQ++D+     QALV+APTRELAQQI+KVM ALG+YL V +  C+GGTSVR+DQR L+AG+HVVVGTPGRV DM+ R +L    IKMFVLDEADEMLSRGFKDQIY++F+ +P  VQV
Sbjct:    7 DVNVSSVVDADGL-IEGNYDQVVESFDDMELKEELLRGIYGFGFEKPSAIQKRAIVPCTTGKDVIAQAQSGTGKTATFSVSILQRIDHEDPHVQALVMAPTRELAQQIQKVMSALGEYLNVNILPCIGGTSVRDDQRKLEAGIHVVVGTPGRVGDMINRNALDTSRIKMFVLDEADEMLSRGFKDQIYEVFRSMPQDVQV 205

HSP 2 Score: 26.5646 bits (57), Expect = 9.745e-67
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP E L++T +FM
Sbjct:  207 LLSATMPSEVLDVTNRFM 224

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_CRYPV (Eukaryotic initiation factor 4A OS=Cryptosporidium parvum GN=EIF4-A PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 2.161e-66
Identity = 124/187 (66.31%), Postives = 149/187 (79.68%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQVGGF 774
            ++YDE+ + F+A+ L+ +LLRGI+AYGFEKPSAIQQRGI P   G D I QAQSGTGKTATF    LQ++DY L  CQ L+LAPTRELAQQI+KV  ALGDY  ++ HACVGGTSVR+D   L++GVH+VVGTPGRVFDML +  LR D +K+F+LDEADEMLSRGFK QI+DIF+ LP  VQV  F
Sbjct:   24 SNYDEIVECFEALNLEGDLLRGIFAYGFEKPSAIQQRGIKPILDGYDTIGQAQSGTGKTATFVIAALQKIDYSLNACQVLLLAPTRELAQQIQKVALALGDYCELRCHACVGGTSVRDDMNKLKSGVHMVVGTPGRVFDMLDKGYLRVDNLKLFILDEADEMLSRGFKVQIHDIFKKLPQDVQVALF 210

HSP 2 Score: 26.1794 bits (56), Expect = 2.161e-66
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            +FSATMP E L +T +FM
Sbjct:  209 LFSATMPNEILHLTTQFM 226

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_USTMA (ATP-dependent RNA helicase eIF4A OS=Ustilago maydis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 6.247e-66
Identity = 122/195 (62.56%), Postives = 150/195 (76.92%), Query Frame = 1

Query:  178 NELLSTDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQ 762
            N L   DG+   ++++ V D+FD M L+E LLRG+YAYGFE+PSAIQ R IVP  KG DVI QAQSGTGKTATF   ILQ++D  +   QAL+LAPTRELAQQI+KV+ ALGDY+ +  HAC+GGT+VRED   L  G  VVVGTPGRV+DM+ R++ + D +KMF LDEADEMLSRGFKDQ+Y++FQLLP   Q
Sbjct:   20 NNLNIADGE-IESNWETVIDNFDNMELKEELLRGVYAYGFERPSAIQARAIVPVIKGHDVIAQAQSGTGKTATFSIAILQRIDPSIKAVQALILAPTRELAQQIQKVVIALGDYMKIDCHACIGGTNVREDMAKLNEGAQVVVGTPGRVYDMINRRAFKTDQLKMFCLDEADEMLSRGFKDQMYEVFQLLPQDTQ 213

HSP 2 Score: 29.6462 bits (65), Expect = 6.247e-66
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP E LE+T+KFM
Sbjct:  216 LLSATMPQEVLEVTKKFM 233

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASPTN (ATP-dependent RNA helicase eIF4A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=tif1 PE=3 SV=2)

HSP 1 Score: 244.973 bits (624), Expect = 6.249e-66
Identity = 116/184 (63.04%), Postives = 150/184 (81.52%), Query Frame = 1

Query:  214 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            ++Y++V DSFD+M L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  L  CQAL++APTRELAQQI+KV+ A+GD++ +  HAC+GGT++R+D + LQ G  +VVGTPGR+ DM++R+ L+ D +K+F+LDEADEMLSRGF +QIYDIFQLLP   QV
Sbjct:   16 SNYEDVTDSFDSMELKAELLRGVYAYGFERPSAIQQRAIMPIIKGNDVIAQAQSGTGKTATFSISALQKIDPNLKACQALIVAPTRELAQQIQKVVVAIGDFMNINCHACIGGTAIRDDMKALQEGPPIVVGTPGRIQDMIQRRVLKTDQMKLFILDEADEMLSRGFTEQIYDIFQLLPQATQV 199

HSP 2 Score: 27.335 bits (59), Expect = 6.249e-66
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP + LE+T KFM
Sbjct:  201 LLSATMPQDVLEVTTKFM 218

The following BLAST results are available for this feature:

BLAST of CV712161 vs. ExPASy Swiss-Prot

BLAST of CV712161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
IF4A_AJECN	3.091e-68	66.85	ATP-dependent RNA helicase eIF4A OS=Ajellomyces ca...	[more]
IF4A2_MACFA	8.935e-68	68.65	Eukaryotic initiation factor 4A-II OS=Macaca fasci...	[more]
IF4A_COCIM	1.165e-67	67.39	ATP-dependent RNA helicase eIF4A OS=Coccidioides i...	[more]
IF4A1_PONAB	2.584e-67	68.65	Eukaryotic initiation factor 4A-I OS=Pongo abelii ...	[more]
IF4A_EMENI	5.728e-67	64.13	ATP-dependent RNA helicase eIF4A OS=Emericella nid...	[more]
IF4A_ASPNC	5.731e-67	65.22	ATP-dependent RNA helicase eIF4A OS=Aspergillus ni...	[more]
IF4A_CAEEL	9.745e-67	64.00	Eukaryotic initiation factor 4A OS=Caenorhabditis ...	[more]
IF4A_CRYPV	2.161e-66	66.31	Eukaryotic initiation factor 4A OS=Cryptosporidium...	[more]
IF4A_USTMA	6.247e-66	62.56	ATP-dependent RNA helicase eIF4A OS=Ustilago maydi...	[more]
IF4A_ASPTN	6.249e-66	63.04	ATP-dependent RNA helicase eIF4A OS=Aspergillus te...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS08_0001A04_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001A04, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV712161 ID=CV712161; Name=CV712161; organism=Citrus sinensis; type=EST; length=825bp
AGTTTGGTAACCCTAGCAACTATTTTCTCTTAAATCAATCTTCTCCTTCT
CTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTTTAATCACCATC
ATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAGGATCTCAATTT
GATGCTCGTCAGTATGACACCAAGATGAATGAACTGCTCTCTACTGATGG
GCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAGTTTTGATGCAA
TGGGACTGCAGGAAAACCTTTTGAGGGGAATCTATGCCTATGGGTTTGAG
AAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACT
TGACGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAACAGCAACTTTCT
GCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCCAATGTCAGGCC
CTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGATTGAGAAAGTAAT
GAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATGCTTGTGTCGGTG
GGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTT
GTTGTTGGCACTCCTGGTCGTGTGTTTGACATGCTGCGCCGACAGTCTTT
ACGCCCTGATTACATTAAGATGTTTGTACTGGATGAGGCTGATGAAATGC
TTTCCCGTGGTTTTAAGGATCAGATCTATGATATTTTCCAGCTGCTTCCA
GCTAAAGTTCAGGTGGGGGGTTTTCTCTGCTACTATGCCTCCTGAGGCCT
TGGAGATTACAAGGAAGTTTATGAA