DT214585

Overview
NameDT214585
Unique NameDT214585
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length906
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1656 contig Csv1_Contig1656:741..1646. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK3_MOUSE (MAP/microtubule affinity-regulating kinase 3 OS=Mus musculus GN=Mark3 PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 1.243e-13
Identity = 48/146 (32.88%), Postives = 73/146 (50.00%), Query Frame = -3 
Query:  491 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGY-----KP---PHFDKEEEVNLDDVDAIFNDSKENLVTEKKEKPVSMNAFELISRTKSFSLE 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+KR L  N   R T+ Q+++D W   G+     KP   P  D  ++  +D +  +    +E   +  K K   + A  L+   KS  L+
Sbjct:  227 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSAELD 372
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK3_HUMAN (MAP/microtubule affinity-regulating kinase 3 OS=Homo sapiens GN=MARK3 PE=1 SV=3)

HSP 1 Score: 77.411 bits (189), Expect = 1.243e-13
Identity = 48/146 (32.88%), Postives = 73/146 (50.00%), Query Frame = -3 
Query:  491 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGY-----KP---PHFDKEEEVNLDDVDAIFNDSKENLVTEKKEKPVSMNAFELISRTKSFSLE 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+KR L  N   R T+ Q+++D W   G+     KP   P  D  ++  +D +  +    +E   +  K K   + A  L+   KS  L+
Sbjct:  250 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELD 395
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK4_MOUSE (MAP/microtubule affinity-regulating kinase 4 OS=Mus musculus GN=Mark4 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.119e-13
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = -3 
Query:  620 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYK----PPHFDKEEE 904
            K YDG   D+WS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + +++R L  N   R T+ Q+++D+W   GY+     P+ + EE+
Sbjct:  230 KKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEED 328
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK4_HUMAN (MAP/microtubule affinity-regulating kinase 4 OS=Homo sapiens GN=MARK4 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.119e-13
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = -3 
Query:  620 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYK----PPHFDKEEE 904
            K YDG   D+WS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + +++R L  N   R T+ Q+++D+W   GY+     P+ + EE+
Sbjct:  230 KKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEED 328
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK1_RAT (Serine/threonine-protein kinase MARK1 OS=Rattus norvegicus GN=Mark1 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.119e-13
Identity = 38/103 (36.89%), Postives = 57/103 (55.34%), Query Frame = -3 
Query:  596 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYKPPHFDKEEEVNLDDVDA 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+K++L  N   R ++ Q+++D W   G++        E  LD  DA
Sbjct:  231 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRVPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYSEPELDLNDA 333
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK1_MOUSE (Serine/threonine-protein kinase MARK1 OS=Mus musculus GN=Mark1 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.119e-13
Identity = 38/103 (36.89%), Postives = 57/103 (55.34%), Query Frame = -3 
Query:  596 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYKPPHFDKEEEVNLDDVDA 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+K++L  N   R ++ Q+++D W   G++        E  LD  DA
Sbjct:  231 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRVPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYSEPELDLNDA 333
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: PAR1_CAEEL (Serine/threonine-protein kinase par-1 OS=Caenorhabditis elegans GN=par-1 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.722e-13
Identity = 36/102 (35.29%), Postives = 54/102 (52.94%), Query Frame = -3 
Query:  599 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYKPPHFDKEEEVNLDDVD 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+K+ L  N   R ++  +++D W   GY+        E   D +D
Sbjct:  341 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPPKDQID 442
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: PAR1_CAEBR (Serine/threonine-protein kinase par-1 OS=Caenorhabditis briggsae GN=par-1 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.722e-13
Identity = 36/102 (35.29%), Postives = 54/102 (52.94%), Query Frame = -3 
Query:  599 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYKPPHFDKEEEVNLDDVD 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+K+ L  N   R ++  +++D W   GY+        E   D +D
Sbjct:  299 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVINPQRRSSLDNIMKDRWMNVGYEDDELKPFIEPPKDQID 400
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MRKA_DICDI (Probable serine/threonine-protein kinase MARK-A OS=Dictyostelium discoideum GN=mrkA PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 6.167e-13
Identity = 33/80 (41.25%), Postives = 52/80 (65.00%), Query Frame = -3 
Query:  665 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEW 904
            K Y+G + DVWS GV+LFVL+ G+LPFD  N + L++KI   +++ PS+ +   K LI R+L  + D R T+ +++   W
Sbjct:  281 KEYNGPSVDVWSMGVVLFVLVTGYLPFDGDNYVELFQKILAGNYTIPSYLTHECKSLISRMLVVDPDKRATMEEIINHPW 360
[Close]
BLAST of DT214585 vs. ExPASy Swiss-Prot
Match: MARK2_RAT (Serine/threonine-protein kinase MARK2 OS=Rattus norvegicus GN=Mark2 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 8.054e-13
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = -3 
Query:  647 KGYDGRAFDVWSCGVILFVLMAGFLPFDESNLMALYRKICRADFSCPSWFSSGAKKLIKRILDPNXDTRMTISQMLEDEWFKKGYK 904
            K YDG   DVWS GVIL+ L++G LPFD  NL  L  ++ R  +  P + S+  + L+K+ L  N   R T+ Q+++D W   G++
Sbjct:  224 KKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHE 309
The following BLAST results are available for this feature:
BLAST of DT214585 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 112
Match NameE-valueIdentityDescription
MARK3_MOUSE1.243e-1332.88MAP/microtubule affinity-regulating kinase 3 OS=Mu... [more]
MARK3_HUMAN1.243e-1332.88MAP/microtubule affinity-regulating kinase 3 OS=Ho... [more]
MARK4_MOUSE2.119e-1336.36MAP/microtubule affinity-regulating kinase 4 OS=Mu... [more]
MARK4_HUMAN2.119e-1336.36MAP/microtubule affinity-regulating kinase 4 OS=Ho... [more]
MARK1_RAT2.119e-1336.89Serine/threonine-protein kinase MARK1 OS=Rattus no... [more]
MARK1_MOUSE2.119e-1336.89Serine/threonine-protein kinase MARK1 OS=Mus muscu... [more]
PAR1_CAEEL4.722e-1335.29Serine/threonine-protein kinase par-1 OS=Caenorhab... [more]
PAR1_CAEBR4.722e-1335.29Serine/threonine-protein kinase par-1 OS=Caenorhab... [more]
MRKA_DICDI6.167e-1341.25Probable serine/threonine-protein kinase MARK-A OS... [more]
MARK2_RAT8.054e-1339.53Serine/threonine-protein kinase MARK2 OS=Rattus no... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_02I16_f2 Parent Washington Navel Orange Red Scale-Infested Rind cDNA Library UCRCS11-2 Citrus sinensis cDNA clone CS_WEd0002b_E08_VN, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DT214585 ID=DT214585; Name=DT214585; organism=Citrus sinensis; type=EST; length=906bp
GGCTTTTAGTTCTGTCCACTTCATTTCGCCAGCGAATAACATTCATTTCC
TATTTCTTGAAAATGTGTTTGTACGCATTCACTTACAGAAACCAAAACAA
ATTAGAAGAATTAACTAATAATTGACCCATCTGCAGTTGTTGTCACGCCC
TTAATTCTTCTTTATTTTCTTCCGCTTGCCAGACAACATCTTTCAGTCCC
GATGAAAATTTTTTGTAGAACTTGTGAAACTCTAGCGTGTCGCCACCAGT
TTTGCGAACCTCCACAATGTGCAAAGTGGGAGCCACCTCAAACACCTCAG
TAGCTACAGAGAGCTGGCCTTTCCTTCCAGTTTTATCACCTTGTAACTTC
ATCTTGTAGTTTCCCTTGCGAACATTAAATCCAAGAGGCTTTGCAGCTTC
CTCAATTTTAGACATGATTTCATTTGGAGGGCGTTGGGAAGTAAAACGAG
TTTCTCGCTTCACAAGGCCCGTTTGTTTCTCAAACAAATTTTCAAGGCTG
AAACTCTTGGTCCTAGAGATTAGCTCGAAAGCATTCATTGATACAGGCTT
CTCTTTCTTCTCAGTTACAAGATTTTCCTTCGAGTCATTGAAGATAGCAT
CAACATCATCGAGATTCACTTCCTCTTCCTTATCAAAATGTGGTGGTTTG
TACCCTTTCTTGAACCATTCATCCTCTAACATTTGAGAAATAGTCATTCG
TGTATCANGGTTTGGATCAAGAATACGCTTGATTAGTTTCTTGGCACCAG
ATGAAAACCATGACGGACAAGAGAAATCAGCCCTGCAAATTTTTCTATAC
AAAGCCATAAGATTCGACTCATCAAAAGGCAAGAACCCAGCCATAAGTAC
AAAGAGAATAACCCCGCAGGACCAAACATCAAATGCTCTACCGTCGTATC
CCTTGT
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