DT214547

Overview
NameDT214547
Unique NameDT214547
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length813
Libraries
Library NameType
Ruby Orange Developing Seed cDNA Library UCRCS09-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3537 contig Csv1_Contig3537:289..1101. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUSU (Hydroxyacylglutathione hydrolase OS=Brucella suis GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   44 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 175
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUSI (Hydroxyacylglutathione hydrolase OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   47 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 178
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUO2 (Hydroxyacylglutathione hydrolase OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=gloB PE=3 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   44 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 175
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUME (Hydroxyacylglutathione hydrolase OS=Brucella melitensis GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   44 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 175
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUMB (Hydroxyacylglutathione hydrolase OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   47 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 178
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUC2 (Hydroxyacylglutathione hydrolase OS=Brucella canis (strain ATCC 23365 / NCTC 10854) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   47 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 178
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUAB (Hydroxyacylglutathione hydrolase OS=Brucella abortus GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   44 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 175
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUA2 (Hydroxyacylglutathione hydrolase OS=Brucella abortus (strain 2308) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   44 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 175
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_BRUA1 (Hydroxyacylglutathione hydrolase OS=Brucella abortus (strain S19) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.785e-12
Identity = 45/141 (31.91%), Postives = 68/141 (48.23%), Query Frame = -3 
Query:  368 IKELGLKLVYAMNTHVHADHVTGTGLIKSK--VPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDY 784
            ++  G  L +   TH H DHV G   +K K  V  +            D  V+ GD+ +FG   ++V +TPGHT G ++Y        P  ++ FTGD L   GCGR  F+G  ++  +     ++  LP DT +Y  H+Y
Sbjct:   47 LERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYY------LPDAKVVFTGDTLFALGCGRL-FEGTPATMFHSL--EKLVALPGDTALYCGHEY 178
[Close]
BLAST of DT214547 vs. ExPASy Swiss-Prot
Match: GLO2_PSEP1 (Hydroxyacylglutathione hydrolase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=gloB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.278e-11
Identity = 47/168 (27.98%), Postives = 74/168 (44.05%), Query Frame = -3 
Query:  242 THVHADHVTGTGLIKSKVPGVXXXXXXXXXXXADLHVEHGDKVSFGDLFLEVRATPGHTLGCVTYVSGEGPDQPQPRMAFTGDALLIRGCGRTDFQGGSSSQLYKSVHSQIFTLPKDTLIYPAHDYKGFTVSTVGEEIQYNPRLTKDEETFKSIMENLNLSYPKMIDI 745
            TH H DHV G   ++                  DL ++ GD+V    +  +V A PGHTLG + + S    DQP   + F+GD L   GCGR  F+G  + +  +   +++  LP+ T +Y AH+Y    +         NP + +  E    +     +S P  I +
Sbjct:   55 THHHNDHVGGVERLRQLTGARVCGPANERIPGRDLALDEGDRVDVLGVTFQVLAVPGHTLGHIAFFS----DQPATPILFSGDTLFAAGCGRM-FEG--TPEQMQPALARLAALPEQTAVYCAHEYTLSNLRFAKAVEPTNPHVQQRFEDVSRLRAENRISLPSTIGL 215
The following BLAST results are available for this feature:
BLAST of DT214547 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
GLO2_BRUSU9.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella suis ... [more]
GLO2_BRUSI9.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella suis ... [more]
GLO2_BRUO29.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella ovis ... [more]
GLO2_BRUME9.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella melit... [more]
GLO2_BRUMB9.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella melit... [more]
GLO2_BRUC29.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella canis... [more]
GLO2_BRUAB9.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella abort... [more]
GLO2_BRUA29.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella abort... [more]
GLO2_BRUA19.785e-1231.91Hydroxyacylglutathione hydrolase OS=Brucella abort... [more]
GLO2_PSEP11.278e-1127.98Hydroxyacylglutathione hydrolase OS=Pseudomonas pu... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS09_45H11_b2 Ruby Orange Developing Seed cDNA Library UCRCS09-2 Citrus sinensis cDNA clone UCRCS09-45H11-O22-1-7.d-polyt, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DT214547 ID=DT214547; Name=DT214547; organism=Citrus sinensis; type=EST; length=813bp
AGATATTCACTTATATCTTATTTTCATCATTTCACTGATCCATACATTCT
TTAGGGGCTTAACAACTTTTTCTTTGTTATTGTTTTGTACTATACCAATT
AAGAGNCATTATATATNATTTGTACAGAGTAACTTGAACAGCAGGTACCG
AGGTTATCACCATTTGGGCATCGCCCAAGGAAGCTCATCAGGCTTTGGAG
GCCAGATCTTGCATTCCACAAACCAAATTTGCCGGAACTGCTATGTCTAT
CATCTTTGGATATGACAGGTTTAAATTTTCCATTATGGATTTGAATGTTT
CCTCATCTTTCGTTAGGCGAGGATTGTACTGAATCTCCTCTCCCACTGTG
CTTACTGTGAATCCCTTGTAATCATGAGCCGGGTAGATTAATGTGTCCTT
CGGCAAAGTAAATATCTGTGAATGCACTGACTTGTAGAGCTGACTTGAAC
TTCCTCCCTGAAAATCAGTCCTCCCGCATCCACGTATCAGTAAGGCATCT
CCAGTGAATGCCATCCTTGGTTGAGGCTGATCAGGTCCTTCACCAGAAAC
ATAGGTAACACAACCCAACGTATGGCCAGGAGTAGCACGAACCTCAAGGA
AGAGATCACCGAAAGAGACTTTATCCCCATGTTCAACATGAAGATCAGCC
TTGGAACCACTTGCTTTTGATATAATAGATTTCACTCCCGGAACCTTACT
CTTAATCAGACCCGTTCCAGTGACATGATCAGCATGTACATGGGTGTTCA
TGGCATATACAAGCTTCAATCCCAGTTCTTTAATAACATTCAGGTCTCTA
TCAACGGTCTTGT
back to top