EY680081

Overview
NameEY680081
Unique NameEY680081
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length897
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_PSEHT (Peptide chain release factor 2 OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 46/158 (29.11%), Postives = 85/158 (53.80%), Query Frame = 1 
Query:  124 LWDDPTKSNEVLVKLADSLKVVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLI 597
            +W++P ++  +  + +    VV  +  L    ++ + + +LA +EA D   F  A     D+++ L+  E  ++  GP+D   A + +++GS G   + W   LL MY+RW + +G++  +V+       G+K AT+ F  EYA+G+L  ETG H L+
Sbjct:   46 VWNEPERAQALGREKSALEAVVETIDTLVTGTDDVEGLLELA-VEAEDQETFDEAQSELADLNEQLEALEFRRMFSGPHDSNDAYLDLQSGSGGTEAQDWCNILLRMYLRWGEAKGFKVELVEATGGDVAGIKGATVRFVGEYAYGWLRTETGVHRLV 202
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ESCF3 (Peptide chain release factor 2 OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOSM (Peptide chain release factor 2 OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOLU (Peptide chain release factor 2 OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOLI (Peptide chain release factor 2 OS=Escherichia coli (strain K12) GN=prfB PE=1 SV=3)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOLC (Peptide chain release factor 2 OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOL6 (Peptide chain release factor 2 OS=Escherichia coli O6 GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOHS (Peptide chain release factor 2 OS=Escherichia coli O9:H4 (strain HS) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLCYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECODH (Peptide chain release factor 2 OS=Escherichia coli (strain K12 / DH10B) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECOBW (Peptide chain release factor 2 OS=Escherichia coli (strain K12 / BW2952) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
The following BLAST results are available for this feature:
BLAST of EY680081 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 188
Match NameE-valueIdentityDescription
RF2_PSEHT7.698e-1629.11Peptide chain release factor 2 OS=Pseudoalteromona... [more]
RF2_ESCF37.698e-1628.02Peptide chain release factor 2 OS=Escherichia ferg... [more]
RF2_ECOSM7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOLU7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOLI7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOLC7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOL67.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOHS7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECODH7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECOBW7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-650-007-C01-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY680081 ID=EY680081; Name=EY680081; organism=Citrus sinensis; type=EST; length=897bp
CCACGCGTCCGGAAAAAGAAGATTGAAGATGCAGTTCTTCGTGCTGAGAG
TTTAGCAACAACAGCACTAGAACTCGAGGAAGCAACAAGAATTAAGCAGG
AAGCGATGGTCCGTAACTATAACCTTTGGGATGACCCAACTAAATCCAAT
GAAGTTCTTGTCAAGCTAGCTGACAGTCTCAAAGTAGTTAATGCTCTCAA
AGATCTACGATATAAGGCTGAGGAAGCAAAGTTGATATCACAGTTGGCTG
AGATGGAAGCTATCGATTATGGGCTTTTCAAGCGAGCATACCGTGCATCT
TTAGATGTGAGCAAGCTATTGGATCAGTATGAGATGTCCAAGCTTCTTCG
TGGGCCAAATGACGTGGAAGGAGCATCTGTAACTATTAAAGCTGGATCAA
ATGGCATCTGTCCTGAGATATGGGCAGAACAACTTCTCAACATGTATGTT
AGATGGGCTGACAAGGAAGGATATAGAGGAAGGGTAGTTGATAAGTGTTG
CTGCAAGAATGGTGGTGTCAAGTCCGCAACTATTGAATTCGAATTTGAGT
ATGCTTTTGGCTATCTTTCCGGAGAGACAGGTGCTCACTGGCTCATTAAT
TTTCCGAATGGTTCTTTTCCTCATGGAGTAAAAACTACGGTTTCCATTCT
GGCTAAAGTTCAGAGGGTAACCCATTTTCTCTTCCCTGGAACCCAAAGCC
CCCCCAAAAACATTTCTGATTACTTTCCTAAAAAATCATTTTTTGGCCAA
AAGCTTCTAAAATCATTTTTTATTTCAAATTACCCTTTTTTTGGGGAATT
GTTTTTCCCAATGTGTATGCCCATTTAGTGCCCCCGGGTGACCCTTTTTT
TGTTAAATATAAAATTGAATTTTTGTCTGAGGAACACATTAACCAAG
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