EY680081

Overview
NameEY680081
Unique NameEY680081
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length897
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO81 (Peptide chain release factor 2 OS=Escherichia coli O81 (strain ED1a) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO7I (Peptide chain release factor 2 OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO57 (Peptide chain release factor 2 OS=Escherichia coli O157:H7 GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO55 (Peptide chain release factor 2 OS=Escherichia coli (strain 55989 / EAEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO45 (Peptide chain release factor 2 OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_ECO27 (Peptide chain release factor 2 OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.698e-16
Identity = 58/207 (28.02%), Postives = 104/207 (50.24%), Query Frame = 1 
Query:   19 KIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLK-VVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPH 624
            +I+D   R++             R+++  A +   ++W++P ++ + L K   SL+ VV+ L  ++   E+   + +LA +EA D   F  A      + + L Q E  ++  G  D     + I+AGS G   + WA  L  MY+RWA+  G++  ++++   +  G+KS TI+   +YA+G+L  ETG H L+    F +G   H
Sbjct:   10 RIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA-QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELA-VEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRH 214
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_THESQ (Peptide chain release factor 2 OS=Thermotoga sp. (strain RQ2) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.005e-15
Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 
Query:  100 EAMVRNYNLWDDPTKSNEVLVKLADSLKVVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLI 597
            E  + + ++WDD  K+ E   KL     +   LK +R   E+ ++  +L++    D  + +       ++   + + E+  +L G  D   A +++  G+ G   + WA+ LL MY+RWA+++G+   +V+    +  G+K ATI  + EYA+GYL  E+G H L+
Sbjct:   37 EKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE---DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLV 199
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_THEP1 (Peptide chain release factor 2 OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=prfB PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.005e-15
Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 
Query:  100 EAMVRNYNLWDDPTKSNEVLVKLADSLKVVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLI 597
            E  + + ++WDD  K+ E   KL     +   LK +R   E+ ++  +L++    D  + +       ++   + + E+  +L G  D   A +++  G+ G   + WA+ LL MY+RWA+++G+   +V+    +  G+K ATI  + EYA+GYL  E+G H L+
Sbjct:   37 EKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE---DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLV 199
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_THEMA (Peptide chain release factor 2 OS=Thermotoga maritima GN=prfB PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.005e-15
Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 
Query:  100 EAMVRNYNLWDDPTKSNEVLVKLADSLKVVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLI 597
            E  + + ++WDD  K+ E   KL     +   LK +R   E+ ++  +L++    D  + +       ++   + + E+  +L G  D   A +++  G+ G   + WA+ LL MY+RWA+++G+   +V+    +  G+K ATI  + EYA+GYL  E+G H L+
Sbjct:   39 EKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE---DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLV 201
[Close]
BLAST of EY680081 vs. ExPASy Swiss-Prot
Match: RF2_THEM4 (Peptide chain release factor 2 OS=Thermosipho melanesiensis (strain BI429 / DSM 12029) GN=prfB PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.313e-15
Identity = 52/221 (23.53%), Postives = 105/221 (47.51%), Query Frame = 1 
Query:   13 KKKIEDAVLRAESXXXXXXXXXXXXRIKQ-EAMVRNYNLWDDPTKSNEVLVKLADSLKVVNALKDLRYKAEEAKLISQLAEMEAIDYGLFKRAYRASLDVSKLLDQYEMSKLLRGPNDVEGASVTIKAGSNGICPEIWAEQLLNMYVRWADKEGYRGRVVDKCCCKNGGVKSATIEFEFEYAFGYLSGETGAHWLIN---FPNGSFPHGVKTTVSILAKVQ 663
            K++I++   R E              +++ E ++   + W+D  ++ E+        K+++ + D+  K E+ +   +L E +A      K+      D+ + +  +E+  +L    D   A ++I  G+ G   + WA  LL MY+RWA++ G+  ++VD    +  G+KSA +  + EY +GYL  E G H L+    F      H    +V+++ +++
Sbjct:    7 KQRIDEVKKRYEDIVKVFHPEDKKKELEELEKLMGESDFWNDQKRAKEISQNAQRIRKIIDDMVDIENKLEDLEAGLELLEEDATFLDTIKQLID---DIERKVKTFELELILNEKFDSSNAYLSIHPGAGGTESQDWASMLLRMYMRWAERRGFDVQIVDYQPGEEAGIKSAMLYIKGEYVYGYLKYERGVHRLVRISPFDANKRRHTSFASVNVMPEIE 224
The following BLAST results are available for this feature:
BLAST of EY680081 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 188
Match NameE-valueIdentityDescription
RF2_ECO817.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECO7I7.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECO577.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECO557.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECO457.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_ECO277.698e-1628.02Peptide chain release factor 2 OS=Escherichia coli... [more]
RF2_THESQ1.005e-1528.31Peptide chain release factor 2 OS=Thermotoga sp. (... [more]
RF2_THEP11.005e-1528.31Peptide chain release factor 2 OS=Thermotoga petro... [more]
RF2_THEMA1.005e-1528.31Peptide chain release factor 2 OS=Thermotoga marit... [more]
RF2_THEM41.313e-1523.53Peptide chain release factor 2 OS=Thermosipho mela... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C1-650-007-C01-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY680081 ID=EY680081; Name=EY680081; organism=Citrus sinensis; type=EST; length=897bp
CCACGCGTCCGGAAAAAGAAGATTGAAGATGCAGTTCTTCGTGCTGAGAG
TTTAGCAACAACAGCACTAGAACTCGAGGAAGCAACAAGAATTAAGCAGG
AAGCGATGGTCCGTAACTATAACCTTTGGGATGACCCAACTAAATCCAAT
GAAGTTCTTGTCAAGCTAGCTGACAGTCTCAAAGTAGTTAATGCTCTCAA
AGATCTACGATATAAGGCTGAGGAAGCAAAGTTGATATCACAGTTGGCTG
AGATGGAAGCTATCGATTATGGGCTTTTCAAGCGAGCATACCGTGCATCT
TTAGATGTGAGCAAGCTATTGGATCAGTATGAGATGTCCAAGCTTCTTCG
TGGGCCAAATGACGTGGAAGGAGCATCTGTAACTATTAAAGCTGGATCAA
ATGGCATCTGTCCTGAGATATGGGCAGAACAACTTCTCAACATGTATGTT
AGATGGGCTGACAAGGAAGGATATAGAGGAAGGGTAGTTGATAAGTGTTG
CTGCAAGAATGGTGGTGTCAAGTCCGCAACTATTGAATTCGAATTTGAGT
ATGCTTTTGGCTATCTTTCCGGAGAGACAGGTGCTCACTGGCTCATTAAT
TTTCCGAATGGTTCTTTTCCTCATGGAGTAAAAACTACGGTTTCCATTCT
GGCTAAAGTTCAGAGGGTAACCCATTTTCTCTTCCCTGGAACCCAAAGCC
CCCCCAAAAACATTTCTGATTACTTTCCTAAAAAATCATTTTTTGGCCAA
AAGCTTCTAAAATCATTTTTTATTTCAAATTACCCTTTTTTTGGGGAATT
GTTTTTCCCAATGTGTATGCCCATTTAGTGCCCCCGGGTGACCCTTTTTT
TGTTAAATATAAAATTGAATTTTTGTCTGAGGAACACATTAACCAAG
back to top