CX289099

Overview
NameCX289099
Unique NameCX289099
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length467
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROMS (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain AS9601) GN=ispH PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.227e-25
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + FR +L  SD YNRRGF    +  + + + Y SD+I  +++NGF YT G + +KLA+++GFCWGVERAV +AYE R+ +P E IWITNEI
Sbjct:    2 DTQAFRRSLHHSDRYNRRGFDSPTKRAQALEEAYQSDLISSIRDNGFTYTKGRLNIKLAQAFGFCWGVERAVAMAYETRRHYPNENIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9301) GN=ispH PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.227e-25
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + FR +L  SD YNRRGF    +  + + + Y SD+I  +++NGF YT G + +KLA+++GFCWGVERAV +AYE R+ +P E IWITNEI
Sbjct:    2 DTQAFRRSLHHSDRYNRRGFDSPTKRAQALEEAYQSDLISSIRDNGFTYTKGRLNIKLAQAFGFCWGVERAVAMAYETRRHYPNENIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_ANAVT (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=ispH PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 7.188e-25
Identity = 47/93 (50.54%), Postives = 67/93 (72.04%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F+  L  S+NYNR+GFGH+ E    +  EY S +I+++++  +    G+V ++LA+++GFCWGVERAV +AYE RK FP E+IWITNEI
Sbjct:    2 DTKTFKRTLQHSENYNRKGFGHQAEVATQLQSEYQSSLIQEIRDRNYTLQRGDVTIRLAQAFGFCWGVERAVAMAYETRKHFPTERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_ANASP (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Anabaena sp. (strain PCC 7120) GN=ispH PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 7.188e-25
Identity = 47/93 (50.54%), Postives = 67/93 (72.04%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F+  L  S+NYNR+GFGH+ E    +  EY S +I+++++  +    G+V ++LA+++GFCWGVERAV +AYE RK FP E+IWITNEI
Sbjct:    2 DTKTFKRTLQHSENYNRKGFGHQAEVATQLQSEYQSSLIQEIRDRNYTLQRGDVTIRLAQAFGFCWGVERAVAMAYETRKHFPTERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNPX (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain WH8102) GN=ispH PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.226e-24
Identity = 48/93 (51.61%), Postives = 67/93 (72.04%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D   F+ +L  S+ YNRRGFG  EE  E + Q Y S +I  +++NG++ + G + V+LAE++GFCWGVERAV +AYE RK +P E++WITNEI
Sbjct:    2 DTHAFKRSLHHSERYNRRGFGRAEEVAESLEQAYQSGLIGTIRDNGYKLSHGRLTVRLAEAFGFCWGVERAVAMAYETRKHYPAERLWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ispH PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.226e-24
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F+  L +SD YNR+GFG   +    +   Y SD+I+ L++NG+    G + ++LAE++GFCWGVERAV IAYE R+ FP+E+IWITNEI
Sbjct:    2 DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAEAFGFCWGVERAVAIAYETRQHFPQERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNE7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942) GN=ispH PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.226e-24
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F+  L +SD YNR+GFG   +    +   Y SD+I+ L++NG+    G + ++LAE++GFCWGVERAV IAYE R+ FP+E+IWITNEI
Sbjct:    2 DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAEAFGFCWGVERAVAIAYETRQHFPQERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNSC (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain CC9605) GN=ispH PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.601e-24
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D   F+ +L  S+ YNRRGFG  EE  E + Q Y S +I  +++NG+    G + V+LAE++GFCWGVERAV +AYE RK +P E++WITNEI
Sbjct:    2 DTHAFKRSLHHSERYNRRGFGRAEEVAESLEQAYQSGLIGTIRDNGYRLEHGRLNVRLAEAFGFCWGVERAVAMAYETRKHYPSERLWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNS9 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain CC9902) GN=ispH PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.601e-24
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D   F+ +L  S+ YNRRGFG  EE  E + Q Y S +I  +++NG++ T G + V LAE++GFCWGVERAV +AYE R+ +P E++WITNEI
Sbjct:    2 DTHAFKRSLHHSERYNRRGFGRAEEVAENLEQAYQSGLIGTIRDNGYKLTHGRLNVHLAEAFGFCWGVERAVAMAYETRRHYPSERLWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM9 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9312) GN=ispH PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.601e-24
Identity = 47/93 (50.54%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + FR +L  SD YNRRGF    +  + + + Y SD+I  +++NGF Y  G + +KLA+++GFCWGVERAV +AYE R+ +P E IWITNEI
Sbjct:    2 DTQAFRRSLHHSDRYNRRGFDSPTKRAQALEEAYQSDLISSIRDNGFTYNKGRLNIKLAKAFGFCWGVERAVAMAYETRRHYPNENIWITNEI 94
The following BLAST results are available for this feature:
BLAST of CX289099 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
ISPH_PROMS3.227e-2551.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM03.227e-2551.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_ANAVT7.188e-2550.544-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_ANASP7.188e-2550.544-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNPX1.226e-2451.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNP61.226e-2451.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNE71.226e-2451.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNSC1.601e-2451.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNS91.601e-2451.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM91.601e-2450.544-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]

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Properties
Property NameValue
Genbank descriptionC02009B04SK IF1 Citrus clementina cDNA clone C02009B04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX289099 ID=CX289099; Name=CX289099; organism=Citrus clementina; type=EST; length=467bp
GAATCCGTGAATCTCCCTCATCATGGCGACGACTCTGCAATTCTGCCGTG
TTTGCGCGCGAACTGACATAGGTTCGCGCAGTAATAATCGCTCCGGAGCG
CTGGCTCCGCCGCGCCGGAGGCCTGTGATCGTCAGGTGCTCCGGTGAAAA
ATCGCCGAGCTCGGCGGCGGCTATTGGTGCCGAATTTGACGCGAAACTGT
TTCGGCATAACTTGACGAGAAGTGATAATTACAATCGGAGAGGATTTGGC
CACAAGGAAGAGACTTTGGAGCTCATGAATCAGGAGTACACAAGTGATAT
TATAAAGAAATTGAAGGAGAATGGATTTGAGTATACTTGGGGAAATGTTA
AGGTGAAGCTGGCCGAATCATATGGCTTTTGCTGGGGAGTTGAGCGTGCT
GTGCAGATTGCTTATGAAGCAAGAAAACAGTTTCCTGAGGAGAAGATCTG
GATTACCAATGAAATCA
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