CX289099

Overview
NameCX289099
Unique NameCX289099
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length467
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_SYNPW (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain WH7803) GN=ispH PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 4.659e-24
Identity = 47/93 (50.54%), Postives = 64/93 (68.82%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D   F+ +L  SD YNRRGFG  EE    + Q Y S +I  +++NG+    G ++V+LA+ +GFCWGVERAV +AYE RK +P E++WITNEI
Sbjct:    2 DTHAFKRSLHHSDRYNRRGFGRAEEVAGSLEQAYQSSLIGSIRDNGYRLNHGRLQVRLAKDFGFCWGVERAVAMAYETRKHYPSERLWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_NOSP7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=ispH PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 4.659e-24
Identity = 46/93 (49.46%), Postives = 68/93 (73.12%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F+ +L  S+NYNR+GFGH+ E    +  EY S++I+++++  +    G+V ++LA+++GFCWGVERAV +AYE R+ FP E IWITNEI
Sbjct:    2 DTKAFKRSLQHSENYNRKGFGHQAEVATQLQSEYQSNLIQEIRDRNYILQRGDVTIRLAQAFGFCWGVERAVAMAYETRQHFPTEHIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROMP (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) GN=ispH PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.038e-23
Identity = 46/93 (49.46%), Postives = 64/93 (68.82%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + FR +L  SD YNRRGF    +  + + + Y SD+I  ++ N F Y  G + +KLA+++GFCWGVERAV +AYE R+ +P+E IWITNEI
Sbjct:    2 DTQAFRRSLHHSDRYNRRGFDSPTKRAQALEEAYQSDLITSIRNNSFTYKKGRLNIKLAQAFGFCWGVERAVAMAYETRRHYPDENIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROMT (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain NATL2A) GN=ispH PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.771e-23
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + F+  L +SD YNRRGFG   +  + + + Y S +I  ++ENG     G +KVKLAE++GFCWGVER+V +AYE RK +P E+IWITNEI
Sbjct:    2 DTQAFKQTLHKSDRYNRRGFGSANKRAQALAEAYQSGLIGSIRENGNLLEHGRLKVKLAEAFGFCWGVERSVAMAYETRKHYPNERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain NATL1A) GN=ispH PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.771e-23
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + F+  L +SD YNRRGFG   +  + + + Y S +I  ++ENG     G +KVKLAE++GFCWGVER+V +AYE RK +P E+IWITNEI
Sbjct:    2 DTQAFKQTLHKSDRYNRRGFGSANKRAQALAEAYQSGLIGSIRENGNLLEHGRLKVKLAEAFGFCWGVERSVAMAYETRKHYPNERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_TRIEI (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Trichodesmium erythraeum (strain IMS101) GN=ispH PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.312e-23
Identity = 45/93 (48.39%), Postives = 67/93 (72.04%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D K F   L +S+NYNR+GFGH +E   LM   Y S++I++++EN +    G V +KLA+++GFCWGVER+V +AYE R+ FP ++IWI+ E+
Sbjct:    2 DTKAFTRALKKSENYNRQGFGHAQEVATLMQSAYKSNLIQQIRENNYTLQRGEVTIKLAKTFGFCWGVERSVAMAYETRQHFPNQQIWISYEL 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROMM (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9313) GN=ispH PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.312e-23
Identity = 46/93 (49.46%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + F+ +L  S+ YNRRGFG   E    + + Y S +I  +++NG+    G ++VKLAE++GFCWGVERAV +AYE R+ +P E+IWITNEI
Sbjct:    2 DTQAFKRSLHHSERYNRRGFGRANEVASNLEKAYQSSLIGSIRDNGYVLQHGRLQVKLAEAFGFCWGVERAVAMAYETRRHYPSERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9303) GN=ispH PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.312e-23
Identity = 46/93 (49.46%), Postives = 65/93 (69.89%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + F+ +L  S+ YNRRGFG   E    + + Y S +I  +++NG+    G ++VKLAE++GFCWGVERAV +AYE R+ +P E+IWITNEI
Sbjct:    2 DTQAFKRSLHHSERYNRRGFGRANEVASNLEKAYQSSLIGSIRDNGYVLQHGRLQVKLAEAFGFCWGVERAVAMAYETRRHYPSERIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9211) GN=ispH PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 5.151e-23
Identity = 45/93 (48.39%), Postives = 67/93 (72.04%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D + F+ +L  SD YNRRGF    +  + + + Y S++I  +++NG+ +  G ++VKLAE++GFCWGVERAV +AYE R+ +P+E IWITNEI
Sbjct:    2 DTQAFKRSLHHSDRYNRRGFESPTKRAQALEKAYQSNLIGSIRDNGYLFEHGRLRVKLAEAFGFCWGVERAVAMAYETRRHYPKESIWITNEI 94
[Close]
BLAST of CX289099 vs. ExPASy Swiss-Prot
Match: ISPH_PROM5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9515) GN=ispH PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.499e-22
Identity = 43/93 (46.24%), Postives = 64/93 (68.82%), Query Frame = 2 
Query:  188 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEI 466
            D +  +H++  S  YNR+GF    +  + + + Y SD+I  ++EN F +  G +K+KLA+S+GFCWGVERAV +AYE R+ +P E IW+TNEI
Sbjct:    2 DTQAIKHSVQHSGRYNRKGFESPTQRAKALGESYQSDLIASIRENNFSFEKGRLKIKLAKSFGFCWGVERAVAMAYETRRHYPNEDIWMTNEI 94
The following BLAST results are available for this feature:
BLAST of CX289099 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
ISPH_SYNPW4.659e-2450.544-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_NOSP74.659e-2449.464-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROMP1.038e-2349.464-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROMT1.771e-2351.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM11.771e-2351.614-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_TRIEI2.312e-2348.394-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROMM2.312e-2349.464-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM32.312e-2349.464-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM45.151e-2348.394-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_PROM51.499e-2246.244-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]

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Properties
Property NameValue
Genbank descriptionC02009B04SK IF1 Citrus clementina cDNA clone C02009B04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX289099 ID=CX289099; Name=CX289099; organism=Citrus clementina; type=EST; length=467bp
GAATCCGTGAATCTCCCTCATCATGGCGACGACTCTGCAATTCTGCCGTG
TTTGCGCGCGAACTGACATAGGTTCGCGCAGTAATAATCGCTCCGGAGCG
CTGGCTCCGCCGCGCCGGAGGCCTGTGATCGTCAGGTGCTCCGGTGAAAA
ATCGCCGAGCTCGGCGGCGGCTATTGGTGCCGAATTTGACGCGAAACTGT
TTCGGCATAACTTGACGAGAAGTGATAATTACAATCGGAGAGGATTTGGC
CACAAGGAAGAGACTTTGGAGCTCATGAATCAGGAGTACACAAGTGATAT
TATAAAGAAATTGAAGGAGAATGGATTTGAGTATACTTGGGGAAATGTTA
AGGTGAAGCTGGCCGAATCATATGGCTTTTGCTGGGGAGTTGAGCGTGCT
GTGCAGATTGCTTATGAAGCAAGAAAACAGTTTCCTGAGGAGAAGATCTG
GATTACCAATGAAATCA
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