CX291040

Overview
NameCX291040
Unique NameCX291040
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length575
Libraries
Library NameType
OF2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_VIBCH (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Vibrio cholerae GN=ispH PE=3 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 6.067e-13
Identity = 42/109 (38.53%), Postives = 66/109 (60.55%), Query Frame = -3 
Query:  145 ICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 471
            IC ATQ RQDA+ +++ E+VD+++VVG  NSSN++ L+E+AE  G P Y ID  + I P                  W  + Q+ IG+T+GAS P++ V ++L ++ E+
Sbjct:  198 ICYATQNRQDAV-RILAEQVDVMVVVGSKNSSNSTRLKELAEKLGTPGYLIDCPQDIDP-----------------EWFVDAQL-IGVTAGASAPEELVNQILDRIKEL 287
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_CLOPS (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Clostridium perfringens (strain SM101 / Type A) GN=ispH PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.067e-13
Identity = 45/118 (38.14%), Postives = 68/118 (57.63%), Query Frame = -3 
Query:  145 NEHFISFNTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 498
            ++  ++FNTIC AT  RQ ++ ++ +E  DL+ V+GG NSSNT+ L EI +     SY I++ K +         L+  E V+          TIGIT+GASTPD  + EV+ K+ E+
Sbjct:  182 SKEIVAFNTICSATDVRQKSVQELSKE-ADLVFVIGGKNSSNTTKLYEICKKECPKSYHIENVKELDE------SLLEDESVK----------TIGITAGASTPDWIINEVISKIKEL 282
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_THEPX (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermoanaerobacter sp. (strain X514) GN=ispH PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.035e-12
Identity = 45/118 (38.14%), Postives = 70/118 (59.32%), Query Frame = -3 
Query:  133 ISFNTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEIKREE 486
            I FNTICDATQ+RQDA  ++  +KVD++ V+GG +SSNT  L++I E     ++ I+  + +             E+V+           IG+T+GASTPD  +E+V+KK+  +K E+
Sbjct:  187 IFFNTICDATQKRQDAADEL-SKKVDVMFVIGGKHSSNTQKLKKICEKNCKNTFHIEDAEELTL-----------EMVKDHE-------IIGVTAGASTPDYVIEDVIKKIRFLKGED 285
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_THEP3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=ispH PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.035e-12
Identity = 45/118 (38.14%), Postives = 70/118 (59.32%), Query Frame = -3 
Query:  133 ISFNTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEIKREE 486
            I FNTICDATQ+RQDA  ++  +KVD++ V+GG +SSNT  L++I E     ++ I+  + +             E+V+           IG+T+GASTPD  +E+V+KK+  +K E+
Sbjct:  187 IFFNTICDATQKRQDAADEL-SKKVDVMFVIGGKHSSNTQKLKKICEKNCKNTFHIEDAEELTL-----------EMVKDHE-------IIGVTAGASTPDYVIEDVIKKIRFLKGED 285
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_MARAV (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.351e-12
Identity = 45/118 (38.14%), Postives = 68/118 (57.63%), Query Frame = -3 
Query:  118 ICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEIKREEALQLA 471
            IC ATQ RQDA+ K +    D++LVVG  NSSN++ L+E+AE  G P+Y ID   +I P                  WL  G+  IG+T+GAS P+  V +V+K++  +  EE ++++
Sbjct:  197 ICYATQNRQDAV-KQLAGDCDIMLVVGSPNSSNSNRLRELAERMGTPAYLIDEAAQIDPA-----------------WL-YGKKAIGVTAGASAPEVLVADVIKRLKALGGEEPVEVS 295
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_XYLFT (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.765e-12
Identity = 44/111 (39.64%), Postives = 64/111 (57.66%), Query Frame = -3 
Query:  145 NTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 477
            N IC ATQ RQDA+ ++  E  DL+LVVG  NSSN++ L E+A+  G+ SY IDS   I P            +++K +        IG+T+GAS P   V+ VL +++E+
Sbjct:  196 NDICYATQNRQDAVRELARE-CDLVLVVGSPNSSNSNRLSELAQREGVASYLIDSAAEIDP----------AWVIDKHH--------IGVTAGASAPQVLVDGVLARLYEL 287
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_XYLFM (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Xylella fastidiosa (strain M12) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.765e-12
Identity = 44/111 (39.64%), Postives = 64/111 (57.66%), Query Frame = -3 
Query:  145 NTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 477
            N IC ATQ RQDA+ ++  E  DL+LVVG  NSSN++ L E+A+  G+ SY IDS   I P            +++K +        IG+T+GAS P   V+ VL +++E+
Sbjct:  196 NDICYATQNRQDAVRELARE-CDLVLVVGSPNSSNSNRLSELAQREGVASYLIDSAAEIDP----------AWVIDKHH--------IGVTAGASAPQVLVDGVLARLYEL 287
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_XYLFA (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Xylella fastidiosa GN=ispH PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.765e-12
Identity = 44/111 (39.64%), Postives = 64/111 (57.66%), Query Frame = -3 
Query:  145 NTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 477
            N IC ATQ RQDA+ ++  E  DL+LVVG  NSSN++ L E+A+  G+ SY IDS   I P            +++K +        IG+T+GAS P   V+ VL +++E+
Sbjct:  196 NDICYATQNRQDAVRELARE-CDLVLVVGSPNSSNSNRLSELAQREGVASYLIDSAAEIDP----------AWVIDKHH--------IGVTAGASAPQVLVDGVLARLYEL 287
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_XYLF2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Xylella fastidiosa (strain M23) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.765e-12
Identity = 44/111 (39.64%), Postives = 64/111 (57.66%), Query Frame = -3 
Query:  145 NTICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 477
            N IC ATQ RQDA+ ++  E  DL+LVVG  NSSN++ L E+A+  G+ SY IDS   I P            +++K +        IG+T+GAS P   V+ VL +++E+
Sbjct:  196 NDICYATQNRQDAVRELARE-CDLVLVVGSPNSSNSNRLSELAQREGVASYLIDSAAEIDP----------AWVIDKHH--------IGVTAGASAPQVLVDGVLARLYEL 287
[Close]
BLAST of CX291040 vs. ExPASy Swiss-Prot
Match: ISPH_VIBVY (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Vibrio vulnificus (strain YJ016) GN=ispH PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 2.305e-12
Identity = 39/109 (35.78%), Postives = 66/109 (60.55%), Query Frame = -3 
Query:  145 ICDATQERQDAMYKMVEEKVDLILVVGGWNSSNTSHLQEIAEDRGIPSYWIDSEKRIGPGNKIAYKLMHGELVEKENWLPNGQITIGITSGASTPDKAVEEVLKKVFEI 471
            IC ATQ RQDA+ ++ ++ VD+++VVG  NSSN++ L+E+AE  G P+Y  D  + I P                  W  +G++ +G+T+GAS P++ V ++L ++ E+
Sbjct:  198 ICYATQNRQDAVRELAKD-VDVVVVVGSKNSSNSTRLKELAEKLGTPAYLTDCPQDIEP-----------------QWF-DGKVKVGVTAGASAPEELVNQILTRIKEL 287
The following BLAST results are available for this feature:
BLAST of CX291040 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 90
Match NameE-valueIdentityDescription
ISPH_VIBCH6.067e-1338.534-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_CLOPS6.067e-1338.144-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_THEPX1.035e-1238.144-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_THEP31.035e-1238.144-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_MARAV1.351e-1238.144-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_XYLFT1.765e-1239.644-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_XYLFM1.765e-1239.644-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_XYLFA1.765e-1239.644-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_XYLF21.765e-1239.644-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_VIBVY2.305e-1235.784-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]

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Properties
Property NameValue
Genbank descriptionC03003C06SK OF2 Citrus clementina cDNA clone C03003C06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX291040 ID=CX291040; Name=CX291040; organism=Citrus clementina; type=EST; length=575bp
GATTAGATGGGCACTAATAAAAGACCCGACAGAGGGCTGTGTTTATAATT
TTACAATATCTAGTGATACAATCACATCGAAATTTATTGATAATGTGTAT
GTTTGGACCTCAGTTTACGCTAATTGCAAGGCTTCTTCACGCTTGATCTC
GAATACCTTTTTAAGGACCTCTTCAACAGCCTTATCTGGGGTGGAGGCAC
CGGATGTTATACCGATTGTGATCTGGCCGTTCGGCAGCCAATTTTCTTTC
TCCACCAGCTCACCATGCATCAATTTATATGCTATTTTGTTTCCGGGGCC
TATTCTCTTCTCACTATCGATCCAGTATGAGGGAATTCCACGGTCTTCTG
CAATCTCTTGAAGGTGTGAGGTATTGCTCGAGTTCCACCCACCAACAACC
AAGATAAGATCGACCTTTTCCTCAACCATCTTATACATTGCATCTTGTCG
CTCTTGTGTAGCATCACAAATTGTGTTGAAGCTTATGAAGTGTTCATTGA
CATTTTCAACCCCAAATTTACGCATCATGGTCTTCTCGACTAGTTTTCCA
ATCTCTTCTGTTTCACCCTTGAGCA
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