DY258069

Overview
NameDY258069
Unique NameDY258069
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length607
Libraries
Library NameType
AbsCOv1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig10000 contig Ccv1_Contig10000:187..793. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 7.612e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 97.0561 bits (240), Expect = 7.612e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 9.941e-20
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 2 
Query:   14 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 238
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 95.9005 bits (237), Expect = 1.696e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 2 
Query:  242 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.893e-19
Identity = 42/78 (53.85%), Postives = 59/78 (75.64%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGEF 469
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG F
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGGNF 78

HSP 2 Score: 92.8189 bits (229), Expect = 1.436e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.099e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 9.305e-18
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 235
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 90.1225 bits (222), Expect = 9.305e-18
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of DY258069 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.215e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 2 
Query:    8 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 238
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 88.9669 bits (219), Expect = 2.073e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = 2 
Query:  236 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 463
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of DY258069 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_CAEEL7.612e-2060.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH9.941e-2061.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
RUB3_ORYSJ2.893e-1953.85NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
NEDD8_RAT1.099e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.099e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.099e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.099e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.099e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ARATH9.305e-1855.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI1.215e-1753.25NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAB2DE07ZM1 AbsCOv1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258069 ID=DY258069; Name=DY258069; organism=Citrus clementina; type=EST; length=607bp
GGGAGGCATGCAGATATTTGTTAAGACCCTTACTGGGAAAACAATTACTT
TGGAAGTTGAGAGCTCAGACACAATTGACAATGTCAAGGCAAAAATCCAA
GACAAGGAAGGAATTCCACCGGATCAGCAGCGTTTGATCTTCGCTGGCAA
ACAGCTTGAGGATGGAAGGACTCTTGCGGATTATAACATCCAGAAGGAGT
CGACCCTTCATCTGGTTCTGCGCCTGAGGGGTGGTATGCAGATATTTGTC
AAGACCTTGACTGGGAAGACTATTACTCTGGAGGTGGAGAGCTCAGATAC
TATTGATAATGTTAAGGCAAAGATTCAGGACAAGGAAGGGATTCCACCAG
ATCAGCAGAGGCTCATCTTTGCTGGGAAGCAGCTTGAGGATGGCCGTACT
TTAGCTGATTATAACATTCAGAAGGAGTCCACCCTCCATCTTGTGCTCCG
CCTTCGTGGTGGTGAATTCTAAGAATGTTATCTGCTTGCCGCCGTGTCTG
TTCTGCTCTGCTGTAGCAGTAATATTTATTTTTCATTTGCTAGTCCTGCT
TCTGTTTCAGTCTTTTTGTCACATCAAGTTTTATGTGGTAATGAATAAAA
ATTAGTT
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