DY258681

Overview
NameDY258681
Unique NameDY258681
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1283
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPD2_HYONI (Spermidine synthase 2 OS=Hyoscyamus niger PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 5.733e-66
Identity = 117/146 (80.14%), Postives = 127/146 (86.99%), Query Frame = 3 
Query:  216 MEMDSNKQPDCISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            ME   N +   ISS++PGWFSEISP+WPGEAHSLKVEKILFQGKSDYQNV+VFQSSTYGKVL+LDGVIQLTERDECAYQEMITHLPLCSIPNPK VLVIGGGDG VLRE+SRHSSVE+ DI EIDKM   V KQFFP+V    +DP
Sbjct:    1 MEEQGNNESAYISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDVSKQFFPNVAIGYEDP 146

HSP 2 Score: 32.7278 bits (73), Expect = 5.733e-66
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = 1 
Query:  637 VG*KIPRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            +G + PRV LH+GDG AF+K   E   + + ++   D + P Q+ L   PFF S+
Sbjct:  141 IGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVD-SSDPIGPAQE-LFEKPFFESV 193
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPD1_ORYSJ (Spermidine synthase 1 OS=Oryza sativa subsp. japonica GN=SPDSYN1 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.808e-64
Identity = 112/135 (82.96%), Postives = 121/135 (89.63%), Query Frame = 3 
Query:  249 ISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            IS+VIPGWFSEISPMWPGEAHSLKVEK+LFQGKSDYQNVMVFQSSTYGKVL+LDGVIQ+TERDECAYQEMITHLPLCSI +PK VLVIGGGDG VLRE+SRHSSVE+ DI EIDKM   V KQFFP +    +DP
Sbjct:   27 ISAVIPGWFSEISPMWPGEAHSLKVEKVLFQGKSDYQNVMVFQSSTYGKVLVLDGVIQVTERDECAYQEMITHLPLCSIKDPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQFFPHLAVGFEDP 161

HSP 2 Score: 35.4242 bits (80), Expect = 6.808e-64
Identity = 23/55 (41.82%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  637 VG*KIPRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            VG + PRV+LHIGDG AF+K   E   + + ++   D + P Q+ L   PFF S+
Sbjct:  156 VGFEDPRVSLHIGDGVAFLKNAPEGTYDAVIVD-SSDPIGPAQE-LFEKPFFQSV 208
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: PMT1_TOBAC (Putrescine N-methyltransferase 1 OS=Nicotiana tabacum GN=PMT1 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 9.589e-44
Identity = 85/161 (52.80%), Postives = 106/161 (65.84%), Query Frame = 3 
Query:  171 DGEKEANNNNNGSVLMEMDSNKQPDCISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            +G +   +      +   + N+      S+ PGWFSE S +WPGEA SLKVEK+LFQGKSDYQ+VM+F+S+TYGKVL LDG IQ TE     Y EMI HLPL SIPNPK VL+IGGG G  L E+ R+ S+EK DI EID +   V ++FFP +     DP
Sbjct:   55 NGHQNGTSEQQNGTISHDNGNELLGSSDSIKPGWFSEFSALWPGEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTEMIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPSIEKIDIVEIDDVVVDVSRKFFPYLAANFNDP 215

HSP 2 Score: 29.6462 bits (65), Expect = 9.589e-44
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            PRVTL +GDG AFVKA +    + + ++   D + P  K L   PFF ++
Sbjct:  215 PRVTLVLGDGAAFVKAAQAGYYDAIIVD-SSDPIGP-AKDLFERPFFEAV 262
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: PMT3_TOBAC (Putrescine N-methyltransferase 3 OS=Nicotiana tabacum GN=PMT3 PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 2.765e-43
Identity = 86/154 (55.84%), Postives = 109/154 (70.78%), Query Frame = 3 
Query:  192 NNNNGSVLMEMDSNKQPDCISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            +++NG+ L  + S+      +S+ PGWFSE S +WPGEA SLKVEK+LFQGKSDYQ+VM+F+S+TYGKVL LDG IQ TE     Y EMI HLPL SIPNPK VL+IGGG G  L E+ R+ ++EK DI EID +   V ++FFP +     DP
Sbjct:   74 SHDNGNELQLLGSS------NSIKPGWFSEFSALWPGEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTEMIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLAANFSDP 221

HSP 2 Score: 29.6462 bits (65), Expect = 2.765e-43
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            PRVTL +GDG AFVKA +    + + ++   D + P  K L   PFF ++
Sbjct:  221 PRVTLVLGDGAAFVKAAQAGYYDAIIVD-SSDPIGP-AKDLFERPFFEAV 268
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: PMT2_TOBAC (Putrescine N-methyltransferase 2 OS=Nicotiana tabacum GN=PMT2 PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 4.699e-43
Identity = 89/178 (50.00%), Postives = 114/178 (64.04%), Query Frame = 3 
Query:  147 LPLKRPRDDGEKEANNNNNGSV-----LMEMDSNKQ----PDCISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            +P+    +   K  N + NG+       + +D+  +     +CI    PGWFSE S +WPGEA SLKVEK+LFQGKSDYQ+VM+F+S+TYGKVL LDG IQ TE     Y EMI HLPL SIPNPK VL+IGGG G  L E+ R+ ++EK DI EID +   V ++FFP +     DP
Sbjct:   19 IPMNGHHNGTSKHQNGHKNGTSEQQNGTISLDNGNELLGNSNCIK---PGWFSEFSALWPGEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTEMIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLAANFNDP 193

HSP 2 Score: 30.0314 bits (66), Expect = 4.699e-43
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            PRVTL +GDG AFVKA +    + + ++   D + P  K L   PFF ++
Sbjct:  193 PRVTLVLGDGAAFVKAAQAEYYDAIIVD-SSDPIGP-AKDLFERPFFEAV 240
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: PMT4_TOBAC (Putrescine N-methyltransferase 4 OS=Nicotiana tabacum GN=PMT4 PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 3.239e-41
Identity = 82/161 (50.93%), Postives = 105/161 (65.22%), Query Frame = 3 
Query:  171 DGEKEANNNNNGSVLMEMDSNKQPDCISSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            +G +   +      +   + N+     +S+  GWFSE S +WPGEA SLKVEK+LFQGKSDYQ+VM+F+S+TYGKVL LDG IQ TE     Y EMI HLPL SIPNPK VL+IGGG G  L E+ R+ ++EK DI EID +   V ++ FP +     DP
Sbjct:   99 NGHQNGTSEQQNGTISHDNGNELLGNSNSIKLGWFSEFSALWPGEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTEMIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKSFPYLAANFNDP 259

HSP 2 Score: 29.6462 bits (65), Expect = 3.239e-41
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRETPVNVLNLEIPYD*MVPRQKILIHHPFFTSL 801
            PRVTL +GDG AFVKA +    + + ++   D + P  K L   PFF ++
Sbjct:  259 PRVTLVLGDGAAFVKAAQAGYYDAIIVD-SSDPIGP-AKDLFERPFFEAV 306
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPEE_SCHPO (Spermidine synthase OS=Schizosaccharomyces pombe GN=SPBC12C2.07c PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 4.588e-40
Identity = 76/129 (58.91%), Postives = 96/129 (74.42%), Query Frame = 3 
Query:  267 GWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            GWF EI+ MWPG+A +LKV+K+L+ GKS YQ+V+VF+S TYG VL+LDG IQ TERDE +YQEMI HL L S PNPK VLVIGGGDG VLRE+ +H  VE+  + +ID+   KV KQ+ P++      P
Sbjct:   14 GWFREINNMWPGQAMTLKVKKVLYAGKSKYQDVLVFESETYGHVLVLDGAIQATERDEFSYQEMIAHLALNSHPNPKKVLVIGGGDGGVLREVVKHECVEEAILCDIDEDVIKVSKQYLPEMSAGFNHP 142

HSP 2 Score: 26.5646 bits (57), Expect = 4.588e-40
Identity = 10/20 (50.00%), Postives = 15/20 (75.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRET 711
            P+V +HIGDGF F++  + T
Sbjct:  142 PKVKVHIGDGFKFLQDYQNT 161
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPEE_YEAST (Spermidine synthase OS=Saccharomyces cerevisiae GN=SPE3 PE=1 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 1.863e-38
Identity = 75/134 (55.97%), Postives = 99/134 (73.88%), Query Frame = 3 
Query:  255 SVIPGWFSEISP-MWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFPDVPGWLKDP 653
            +++ GWF EIS  MWPG+A +LKVEK+L   KS YQ+V++F+S+TYG VL+LD VIQ TERDE AYQEMI HL L S PNPK VLVIGGGDG VLRE+ +H SVE+  + +ID+   ++ K++ P++      P
Sbjct:    9 TIVDGWFREISDTMWPGQAMTLKVEKVLHHEKSKYQDVLIFKSTTYGNVLVLDNVIQATERDEFAYQEMIAHLALNSHPNPKKVLVIGGGDGGVLREVVKHDSVEEAWLCDIDEAVIRLSKEYLPEMAASYSHP 142

HSP 2 Score: 26.1794 bits (56), Expect = 1.863e-38
Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  652 PRVTLHIGDGFAFVKAGRET 711
            P+V  HIGDGF F++  + T
Sbjct:  142 PKVKTHIGDGFQFLRDYQNT 161
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPEE_MOUSE (Spermidine synthase OS=Mus musculus GN=Srm PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 6.989e-38
Identity = 71/125 (56.80%), Postives = 94/125 (75.20%), Query Frame = 3 
Query:  252 SSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFP 626
            +++  GWF E   +WPG+A SL+VE++L   +S YQ+++VF+S TYG VL+LDGVIQ TERDE +YQEMI +LPLCS PNP+ VL+IGGGDG VLRE+ +H SVE     EID+   +V K+F P
Sbjct:   14 AAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIEVSKKFLP 138

HSP 2 Score: 28.8758 bits (63), Expect = 6.989e-38
Identity = 11/24 (45.83%), Postives = 17/24 (70.83%), Query Frame = 1 
Query:  637 VG*KIPRVTLHIGDGFAFVKAGRE 708
            VG    ++TLH+GDGF F+K  ++
Sbjct:  142 VGFSSSKLTLHVGDGFEFMKQNQD 165
[Close]
BLAST of DY258681 vs. ExPASy Swiss-Prot
Match: SPEE_HUMAN (Spermidine synthase OS=Homo sapiens GN=SRM PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 9.105e-38
Identity = 71/125 (56.80%), Postives = 94/125 (75.20%), Query Frame = 3 
Query:  252 SSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKNVLVIGGGDGEVLREISRHSSVEKNDIQEIDKMAFKVPKQFFP 626
            +++  GWF E   +WPG+A SL+VE++L   +S YQ+++VF+S TYG VL+LDGVIQ TERDE +YQEMI +LPLCS PNP+ VL+IGGGDG VLRE+ +H SVE     EID+   +V K+F P
Sbjct:   14 AAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLP 138

HSP 2 Score: 28.4906 bits (62), Expect = 9.105e-38
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = 1 
Query:  637 VG*KIPRVTLHIGDGFAFVKAGRE 708
            +G    ++TLH+GDGF F+K  ++
Sbjct:  142 IGYSSSKLTLHVGDGFEFMKQNQD 165
The following BLAST results are available for this feature:
BLAST of DY258681 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 203
Match NameE-valueIdentityDescription
SPD2_HYONI5.733e-6680.14Spermidine synthase 2 OS=Hyoscyamus niger PE=2 SV=... [more]
SPD1_ORYSJ6.808e-6482.96Spermidine synthase 1 OS=Oryza sativa subsp. japon... [more]
PMT1_TOBAC9.589e-4452.80Putrescine N-methyltransferase 1 OS=Nicotiana taba... [more]
PMT3_TOBAC2.765e-4355.84Putrescine N-methyltransferase 3 OS=Nicotiana taba... [more]
PMT2_TOBAC4.699e-4350.00Putrescine N-methyltransferase 2 OS=Nicotiana taba... [more]
PMT4_TOBAC3.239e-4150.93Putrescine N-methyltransferase 4 OS=Nicotiana taba... [more]
SPEE_SCHPO4.588e-4058.91Spermidine synthase OS=Schizosaccharomyces pombe G... [more]
SPEE_YEAST1.863e-3855.97Spermidine synthase OS=Saccharomyces cerevisiae GN... [more]
SPEE_MOUSE6.989e-3856.80Spermidine synthase OS=Mus musculus GN=Srm PE=2 SV... [more]
SPEE_HUMAN9.105e-3856.80Spermidine synthase OS=Homo sapiens GN=SRM PE=1 SV... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI3AA08FM1 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258681 ID=DY258681; Name=DY258681; organism=Citrus clementina; type=EST; length=1283bp
GGTGTCAAGACACTTAATAGTTGCCGCGGCATCCATGTAACGAAATACGA
GATCTGAATGATGGGTTTTCGGGGGTTTGAAAGCAGAAAGAATAATCAAT
AATAATATCTGTTATGGCGGAAACTGGGTCTGCTGCTGCCACTGATTTGC
CGTTGAAGAGGCCGAGAGATGACGGAGAGAAGGAGGCTAATAACAACAAT
AACGGGAGTGTGTTGATGGAGATGGACTCTAACAAACAGCCTGATTGTAT
CTCCTCTGTTATTCCTGGCTGGTTCTCTGAAATCAGCCCAATGTGGCCCG
GTGAGGCACATTCATTGAAGGTTGAGAAAATCTTGTTTCAAGGGAAGTCT
GATTACCAGAATGTCATGGTCTTTCAGTCTTCAACATATGGGAAGGTTCT
TATTTTGGATGGGGTGATTCAGCTTACTGAGAGGGATGAATGTGCATATC
AAGAAATGATCACTCACCTTCCACTTTGTTCAATTCCCAACCCGAAAAAC
GTTCTGGTAATTGGAGGAGGAGATGGTGAGGTCTTGCGGGAAATATCTCG
TCATTCTTCTGTTGAGAAAAATGACATCCAGGAAATTGATAAAATGGCCT
TTAAGGTCCCAAAACAATTTTTCCCTGATGTACCTGGTTGGTTGAAAGAT
CCCCCGGGTGACCCTCCATATTGGTGATGGGTTTGCTTTCGTGAAGGCCG
GTCGGGAAACCCCAGTCAACGTACTTAACCTCGAAATTCCTTACGACTAA
ATGGTCCCTCGCCAAAAGATCCTTATCCACCACCCATTCTTTACCTCGCT
CCCCCCTCCTTACCCCCCCCCACGCGGGCTACCCCAACACTCCCCAGCGG
GACACCCCCCCCTCTGGAACTCCCAGAATCGAACAACATACTGTACCCAT
CCTAACCACCAACCTCATCAATATCCGCCCCATCTCCGCGACGACTCCAC
CGCCACCAACCACACACACACTCGCTAGTCTTTCACTCCTCCTCCTCTCC
CTCTCAACATACCCTTTTTTTCCTCTCACTTTTTCCTCATCTCTTCCCAC
TCCCTCCTAACCCACCTAGTTTCTACACTATTAAATCCTCACGACATCGC
CCATTACTCATNCCCATGAATTCCGTCTTAACCCCTCCCTCTCCCGGACA
GACACGATGCCTGAGTCAAACTACCTCACCGCCCATCTCCCCGCTTACCC
CACCGCCCACACCTACAATCCACCCTACACTAACCCCCCTTTCCGCTCTA
TAACATTAGACAACTATATTGACCTCTCCTTTT
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