DY259883

Overview
NameDY259883
Unique NameDY259883
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length929
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_RICPR (60 kDa chaperonin OS=Rickettsia prowazekii GN=groL PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.895e-13
Identity = 40/105 (38.10%), Postives = 65/105 (61.90%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            D ++G  +A++A+V  +  +++ I S E+IA  GT ++NGD+ IGE IAKA E+     +  + + K   S ++++  GM  DRGYLSPYF+TN +     L +P
Sbjct:  115 DLKRGMDLAVNAVVEEIKRSSKKINSQEEIAQVGTISSNGDKEIGEKIAKAMEEVGKEGVITVEEAKN-FSFDVEVVKGMMFDRGYLSPYFVTNSEKMVAELENP 218

HSP 2 Score: 22.3274 bits (46), Expect = 3.895e-13
Identity = 8/14 (57.14%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  559 EIPYIMILEKKVSN 600
            E P+I++ EKK+SN
Sbjct:  216 ENPFILLFEKKLSN 229
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_SPHWW (60 kDa chaperonin OS=Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) GN=groL PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.895e-13
Identity = 39/105 (37.14%), Postives = 60/105 (57.14%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            D ++G  +A+  +V NL + ++P+    ++A  G  +ANGD+ +GE IA+A +K  G    +  +       EL +  GM  DRGYLSPYFITN +  +  L DP
Sbjct:  115 DLKRGIDLAVTKVVENLKARSKPVAGSNEVAQVGIISANGDKEVGEKIAEAMDK-VGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMQVELTDP 218

HSP 2 Score: 22.7126 bits (47), Expect = 3.895e-13
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 1 
Query:  565 PYIMILEKKVSN 600
            PYI+I EKK+SN
Sbjct:  218 PYILIHEKKLSN 229
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_PROA2 (60 kDa chaperonin OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) GN=groL PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.895e-13
Identity = 41/105 (39.05%), Postives = 60/105 (57.14%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            D ++G  +A+  +V  L   ++ IT  ++IA  GT +AN D  IGELIA+A +K  G +  +  +       EL +  GM  DRGYLSPYF+TN +  +  L DP
Sbjct:  115 DLKRGIDLAVKDVVAELREISRDITGKKEIAQVGTISANNDPEIGELIAEAMDK-VGKDGVITVEEAKGMDTELTVVEGMQFDRGYLSPYFVTNSEKMDADLEDP 218

HSP 2 Score: 23.8682 bits (50), Expect = 3.895e-13
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  559 EIPYIMILEKKVSN 600
            E PYI+I +KK+SN
Sbjct:  216 EDPYILIYDKKISN 229
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH601_BRAJA (60 kDa chaperonin 1 OS=Bradyrhizobium japonicum GN=groL1 PE=2 SV=3)

HSP 1 Score: 73.1738 bits (178), Expect = 3.897e-13
Identity = 39/105 (37.14%), Postives = 61/105 (58.10%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            D ++G  +A++A+V +L + A+ +T+ E+IA   T +ANGD  IG  +A A +K  GN+  +  +       EL++  GM  DRGY SPYF+TN +       DP
Sbjct:  115 DLKRGIDLAVEAIVNDLKAHAKKVTTNEEIAQIATISANGDIEIGRFLADAMQKV-GNDGVITVEEAKSLDTELEVVEGMQFDRGYASPYFVTNAEKMRVEFEDP 218

HSP 2 Score: 23.0978 bits (48), Expect = 3.897e-13
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  541 RKNVFYEIPYIMILEKKVS 597
            +  V +E PYI+I EKK+S
Sbjct:  210 KMRVEFEDPYILIHEKKLS 228
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_TRYBB (Chaperonin HSP60, mitochondrial OS=Trypanosoma brucei brucei GN=HSP60 PE=2 SV=2)

HSP 1 Score: 75.485 bits (184), Expect = 4.879e-13
Identity = 38/104 (36.54%), Postives = 66/104 (63.46%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRD 563
            D ++G   A++ ++ N+ S ++ +T+ E +    T +ANGD  +G+LI +A EK   + +    D KT  + EL++  GM++DRGY+SPYF+T+ KT++  L D
Sbjct:  122 DMKRGMDRAVEVILKNIESQSRTVTNTENVVQVATISANGDVELGKLIGEAMEKVGKDGVITTQDGKTLTT-ELEVVEGMSVDRGYISPYFVTDAKTQKAELED 224
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_CHLL2 (60 kDa chaperonin OS=Chlorobium limicola (strain DSM 245 / NBRC 103803) GN=groL PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.879e-13
Identity = 42/106 (39.62%), Postives = 65/106 (61.32%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPL 569
            D ++G   A+  +V  L + ++ I+  ++IA  GT +AN D  IGELIA+A +K   + +  + + K   + EL++  GM  DRGYLSPYF+TN +T E  + DPL
Sbjct:  115 DLKRGIDRAVKEVVAELRNISRSISGKKEIAQVGTISANNDPEIGELIAEAMDKVGKDGVITVEEAKGMDT-ELKVVEGMQFDRGYLSPYFVTNPETMEAEIEDPL 219
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH602_FRASC (60 kDa chaperonin 2 OS=Frankia sp. (strain CcI3) GN=groL2 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.879e-13
Identity = 37/103 (35.92%), Postives = 64/103 (62.14%), Query Frame = 3 
Query:  258 RQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            ++G  +A++ + G L + ++ + + +QIA   + +A GD  IG++IA+A +K  G +  +  +      +EL+L  GM  D+GY+SPYFIT+ +  ECVL DP
Sbjct:  116 KRGIELAVERVCGELANLSRELETKDQIASTASISAGGDTAIGQIIAEAMDKV-GRDGVITVEESNTFGLELELTEGMRFDKGYISPYFITDQERMECVLEDP 217
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_VARPS (60 kDa chaperonin OS=Variovorax paradoxus (strain S110) GN=groL PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.058e-13
Identity = 44/118 (37.29%), Postives = 66/118 (55.93%), Query Frame = 3 
Query:  213 KGEFYMREDAEVPDQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP 566
            +G  Y+       D ++G   A+ ALV  L   ++P T+ ++IA  G+ +AN D  IG+LIA A +K     +  + D K+  S EL +  GM  DRGYLSPYFI N + +  +L +P
Sbjct:  102 EGFKYVAAGINPMDLKRGIDKAVTALVEELKKASKPTTTSKEIAQVGSISANSDETIGKLIADAMDKVGKEGVITVEDGKSLDS-ELDVVEGMQFDRGYLSPYFINNPEKQSALLDNP 218

HSP 2 Score: 21.557 bits (44), Expect = 5.058e-13
Identity = 5/20 (25.00%), Postives = 15/20 (75.00%), Query Frame = 1 
Query:  541 RKNVFYEIPYIMILEKKVSN 600
            +++   + P++++ +KK+SN
Sbjct:  210 KQSALLDNPFVLLFDKKISN 229
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_PELPB (60 kDa chaperonin OS=Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) GN=groL PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 5.059e-13
Identity = 42/106 (39.62%), Postives = 65/106 (61.32%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDPL 569
            D ++G   A+  +V  L + ++ I+  ++IA  GT +AN D VIG+LIA A +K   + +  + + K   + EL++  GM  DRGYLSPYF+TN +T +  L DPL
Sbjct:  115 DLKRGIDRAVKEVVQELRNISRSISGKKEIAQVGTISANNDPVIGDLIADAMDKVGKDGVITVEEAKGMDT-ELKVVEGMQFDRGYLSPYFVTNSETMDAELEDPL 219

HSP 2 Score: 20.7866 bits (42), Expect = 5.059e-13
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  559 EIPYIMILEKKVSN 600
            E P I+I +KK+SN
Sbjct:  216 EDPLILIYDKKISN 229
[Close]
BLAST of DY259883 vs. ExPASy Swiss-Prot
Match: CH60_THICR (60 kDa chaperonin OS=Thiomicrospira crunogena (strain XCL-2) GN=groL PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.777e-13
Identity = 41/111 (36.94%), Postives = 61/111 (54.95%), Query Frame = 3 
Query:  252 DQRQGARMAIDALVGNLYSTAQPITSFEQIALFGTAAANGDRVIGELIAKAFEKGWGNNLHVLADRKTPPSIELQLYWGMTLDRGYLSPYFITNHKTKECVLRDP--LYHD 578
            D  +G   A++A+V  +   + P T+ E IA  GT +AN D  +G++IA+A EK     + +  +  +    EL +  GM  DRGYLSPYF+TN +     L +P  L HD
Sbjct:  114 DLNRGIHKAVEAVVKEIQKMSVPCTTTESIAQVGTISANSDSAVGKMIAEAMEKVSTEGV-ITVEEGSSLHDELVVVEGMEFDRGYLSPYFVTNQEKMVAELDNPYILLHD 223

HSP 2 Score: 23.483 bits (49), Expect = 5.777e-13
Identity = 14/35 (40.00%), Postives = 21/35 (60.00%), Query Frame = 2 
Query:  614 DLLAEIEPFRKYNGTNVLIIAKKLKNDLLTSLVLN 718
            DLL  +E   K  G  +LIIA+ +  + L +LV+N
Sbjct:  231 DLLPTLEAVSKA-GRPLLIIAEDVDGEALATLVIN 264

HSP 3 Score: 21.557 bits (44), Expect = 5.777e-13
Identity = 7/12 (58.33%), Postives = 11/12 (91.67%), Query Frame = 1 
Query:  565 PYIMILEKKVSN 600
            PYI++ +KK+SN
Sbjct:  217 PYILLHDKKISN 228
The following BLAST results are available for this feature:
BLAST of DY259883 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 296
Match NameE-valueIdentityDescription
CH60_RICPR3.895e-1338.1060 kDa chaperonin OS=Rickettsia prowazekii GN=groL... [more]
CH60_SPHWW3.895e-1337.1460 kDa chaperonin OS=Sphingomonas wittichii (strai... [more]
CH60_PROA23.895e-1339.0560 kDa chaperonin OS=Prosthecochloris aestuarii (s... [more]
CH601_BRAJA3.897e-1337.1460 kDa chaperonin 1 OS=Bradyrhizobium japonicum GN... [more]
CH60_TRYBB4.879e-1336.54Chaperonin HSP60, mitochondrial OS=Trypanosoma bru... [more]
CH60_CHLL24.879e-1339.6260 kDa chaperonin OS=Chlorobium limicola (strain D... [more]
CH602_FRASC4.879e-1335.9260 kDa chaperonin 2 OS=Frankia sp. (strain CcI3) G... [more]
CH60_VARPS5.058e-1337.2960 kDa chaperonin OS=Variovorax paradoxus (strain ... [more]
CH60_PELPB5.059e-1339.6260 kDa chaperonin OS=Pelodictyon phaeoclathratifor... [more]
CH60_THICR5.777e-1336.9460 kDa chaperonin OS=Thiomicrospira crunogena (str... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP10YI01FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY259883 ID=DY259883; Name=DY259883; organism=Citrus clementina; type=EST; length=929bp
TTCTGTGTCATGTTCATTTAACTCGGAGCAGACAGTATACCGCGGCGGAA
GTCACTAAAAGATTTTTGGATTGTGAAACATCTTCTGGATTTTCTTAATT
AAGAAATTAGATTTGCTGTGAATGGGAATGGAGTTAATTAATGGATACAG
ATCATCTGATCAAATTCAATTGACATCTATCTTCTGTCTGCAGGCACCAA
TTCTAAAGTTAAAAGGGAGAATTTTATATGCGGGAGGATGCGGAAGTGCC
AGACCAAAGACAAGGGGCTAGAATGGCAATTGATGCACTTGTGGGAAATT
TATACAGCACAGCACAACCCATCACATCATTCGAACAAATAGCCCTGTTT
GGAACGGCTGCAGCAAATGGTGACAGAGTGATTGGCGAGCTCATAGCCAA
GGCTTTTGAGAAAGGTTGGGGAAACAATCTTCATGTGTTAGCTGATCGGA
AAACACCACCCAGTATTGAACTTCAGTTGTATTGGGGAATGACCCTAGAC
AGGGGCTATTTGTCACCTTACTTCATCACCAATCATAAGACGAAAGAATG
TGTTTTACGAGATCCCTTATATCATGATCCTTGAGAAAAAGGTTTCAAAC
TGCGTTCTTCTTTGACTTATTGGCAGAGATTGAACCATTTAGAAAATATA
ATGGCACAAATGTACTGATCATTGCGAAAAAATTGAAAAATGACCTCCTA
ACAAGTCTGGTCCTAAATGGAAAGTCCTACAGACAACAAAGGGCTGGGTC
CAACAACCATCTGGAATTTGGAAAAACTACGAAGGCCAATCCTTTGGGAA
CATAGTCAAATTCCCTTAAGGGGACCGTCAGGGCCAGGAAACAATTTTTT
CACCGCCCCCCCCCGGCCCCCCCCTCCCAAAACCCCCGCCATCTCACACC
ACCGAACGTGTTCCTCCTTTTTGGTACGC
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