Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus

Publication Overview
TitleDevelopment of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus
AuthorsOllitrault F, Terol J, Pina JA, Navarro L, Talon M, Ollitrault P
TypeJournal Article
Journal NameAmerican Journal of Botany
Volume97
Issue11
Year2010
Page(s)e124-e129
CitationOllitrault F, Terol J, Pina JA, Navarro L, Talon M, Ollitrault P. Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus. American journal of botany. 2010; 97(11):e124-e129.

Abstract

Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. METHODS: and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. CONCLUSIONS: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.
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Pages

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Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date2010
Publication TypeJournal Article
Journal AbbreviationAm J Bot
KeywordsCitrus clementina, DNA, bacterial artificial chromosomes, capillary electrophoresis, chromosome mapping, genotype, lemons, microsatellite repeats, polyacrylamide gel electrophoresis, silver