DY303083

Overview
NameDY303083
Unique NameDY303083
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length925
Libraries
Library NameType
CitNFLcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_SOYBN (Amidophosphoribosyltransferase, chloroplastic OS=Glycine max GN=PUR1 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 9.089e-73
Identity = 85/105 (80.95%), Postives = 92/105 (87.62%), Query Frame = 1 
Query:  307 KPREECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPGDMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            KPREECGVVGIYGDPEASRLC LALHA+QHRG EGAGIVAV++  LQS+TGVGLVSDVF QSKL +LPG  AIGH RYSTAG SMLKNVQPF+A YRF +V VAH
Sbjct:   76 KPREECGVVGIYGDPEASRLCSLALHALQHRGQEGAGIVAVHDNHLQSVTGVGLVSDVFEQSKLSRLPGTSAIGHVRYSTAGQSMLKNVQPFLADYRFAAVAVAH 180

HSP 2 Score: 115.931 bits (289), Expect = 9.089e-73
Identity = 59/80 (73.75%), Postives = 67/80 (83.75%), Query Frame = 3 
Query:  624 VNYNTLRAKLE-DNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            VNY +LRA+LE +NGSIFNT+SDT VVLHLI  SK RPF LRIVD CE L+GAYS+VFVTEDKL AVRDP+GF  LV G+
Sbjct:  185 VNYRSLRARLEHNNGSIFNTTSDTEVVLHLIATSKHRPFLLRIVDACEHLQGAYSLVFVTEDKLVAVRDPFGFRPLVMGR 264

HSP 3 Score: 27.335 bits (59), Expect = 9.089e-73
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 1 
Query:  856 GRKSNGALGFASETCA 903
            GR++NGA+  ASETCA
Sbjct:  263 GRRTNGAVVLASETCA 278
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_VIGAC (Amidophosphoribosyltransferase, chloroplastic (Fragment) OS=Vigna aconitifolia GN=PUR1 PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.811e-42
Identity = 106/210 (50.48%), Postives = 121/210 (57.62%), Query Frame = 1 
Query:  307 KPREECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPGDMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAHXXXXXXXXXXXXXM-----------DPFSILVLILWLFSI*FPYRKLGRSF*GLWMFVRSLRVLIQWCLLLRISLVRFVTRMGLMHW*RGRKSNGALGFASETCA 903
            KPREECGVVGIYGDPEASRLC LALHA+QHRG EGAGIVAV++ +   + GVGLVSDVFN++KL +LPG  AIGH RYSTAG S L NVQPFVAGYRFGSV VAH             +           D   +L LI       F  R +            +L+       L    LV  V   G      GR+ NGA+ FASETCA
Sbjct:   14 KPREECGVVGIYGDPEASRLCSLALHALQHRGQEGAGIVAVHDNLFHQVNGVGLVSDVFNEAKLSELPGSCAIGHVRYSTAGHSKLVNVQPFVAGYRFGSVAVAHNGNFVNYRSLRAKLEDNGSIFNTTSDTEVVLHLIATSKHRPFLLRVVDA--------CENLKGAYSLVFLTEDKLVA-VRDFGFRPLVMGRRKNGAVVFASETCA 214
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAAN (Amidophosphoribosyltransferase OS=Staphylococcus aureus (strain N315) GN=purF PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 5.790e-33
Identity = 47/103 (45.63%), Postives = 72/103 (69.90%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  +   L+   G+GL+++  N  ++++L G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 60.8474 bits (146), Expect = 5.790e-33
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            L+N  +LR  LE  G+IF++SSDT V++HLI  SKA  F   + +   K++G ++   +T+D L    DP     LV GK
Sbjct:  115 LINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGK 194
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAAM (Amidophosphoribosyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=purF PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 5.790e-33
Identity = 47/103 (45.63%), Postives = 72/103 (69.90%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  +   L+   G+GL+++  N  ++++L G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 60.8474 bits (146), Expect = 5.790e-33
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            L+N  +LR  LE  G+IF++SSDT V++HLI  SKA  F   + +   K++G ++   +T+D L    DP     LV GK
Sbjct:  115 LINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGK 194
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAES (Amidophosphoribosyltransferase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=purF PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 7.541e-33
Identity = 46/103 (44.66%), Postives = 70/103 (67.96%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  N++ L+   G+GL+++      +  + G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNHETLKGERGLGLLTEAIKDEHMSNIKGYPHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 62.3882 bits (150), Expect = 7.541e-33
Identity = 33/82 (40.24%), Postives = 49/82 (59.76%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGKEE 866
            L+N  +LR  LE+ G+IF++SSDT V++HLI  SKA  F   + +    ++G ++   +T+D L  V DP     LV GK E
Sbjct:  115 LINAKSLRQNLEEQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRLIKGGFTFAILTKDALYGVVDPNAIRPLVVGKME 196
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAEQ (Amidophosphoribosyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=purF PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 7.541e-33
Identity = 46/103 (44.66%), Postives = 70/103 (67.96%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  N++ L+   G+GL+++      +  + G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNHETLKGERGLGLLTEAIKDEHMSNIKGYPHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 62.3882 bits (150), Expect = 7.541e-33
Identity = 33/82 (40.24%), Postives = 49/82 (59.76%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGKEE 866
            L+N  +LR  LE+ G+IF++SSDT V++HLI  SKA  F   + +    ++G ++   +T+D L  V DP     LV GK E
Sbjct:  115 LINAKSLRQNLEEQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRLIKGGFTFAILTKDALYGVVDPNAIRPLVVGKME 196
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAAW (Amidophosphoribosyltransferase OS=Staphylococcus aureus (strain MW2) GN=purF PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.825e-32
Identity = 46/103 (44.66%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  +   L+   G+GL+++     ++++L G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 60.8474 bits (146), Expect = 2.825e-32
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            L+N  +LR  LE  G+IF++SSDT V++HLI  SKA  F   + +   K++G ++   +T+D L    DP     LV GK
Sbjct:  115 LINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGK 194
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAAS (Amidophosphoribosyltransferase OS=Staphylococcus aureus (strain MSSA476) GN=purF PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.825e-32
Identity = 46/103 (44.66%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  +   L+   G+GL+++     ++++L G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 60.8474 bits (146), Expect = 2.825e-32
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            L+N  +LR  LE  G+IF++SSDT V++HLI  SKA  F   + +   K++G ++   +T+D L    DP     LV GK
Sbjct:  115 LINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGK 194
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_STAAC (Amidophosphoribosyltransferase OS=Staphylococcus aureus (strain COL) GN=purF PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.825e-32
Identity = 46/103 (44.66%), Postives = 71/103 (68.93%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVFNQSKLDQLPG-DMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV GI+  PEA++L Y+ LH++QHRG EGAGIV  +   L+   G+GL+++     ++++L G   AIGH RY+T+G+  ++N+QPF+  +   SVG+ H
Sbjct:    9 EECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICH 111

HSP 2 Score: 60.8474 bits (146), Expect = 2.825e-32
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKGK 860
            L+N  +LR  LE  G+IF++SSDT V++HLI  SKA  F   + +   K++G ++   +T+D L    DP     LV GK
Sbjct:  115 LINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGK 194
[Close]
BLAST of DY303083 vs. ExPASy Swiss-Prot
Match: PUR1_LACCA (Amidophosphoribosyltransferase (Fragment) OS=Lactobacillus casei GN=purF PE=3 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.743e-32
Identity = 47/103 (45.63%), Postives = 70/103 (67.96%), Query Frame = 1 
Query:  316 EECGVVGIYGDPEASRLCYLALHAMQHRGLEGAGIVAVNNKVLQSITGVGLVSDVF-NQSKLDQLPGDMAIGHARYSTAGSSMLKNVQPFVAGYRFGSVGVAH 621
            EECGV G++G+P A+ + +L LH +QHRG EGAGIV +    ++   G+GL+S+VF N  +L  L G  A+GH RYSTAG  +L+N+QP +  +   ++ +AH
Sbjct:   10 EECGVFGVWGNPNAASITHLGLHTLQHRGQEGAGIVGLTKDGMRRHYGLGLLSEVFTNTDQLTPLIGRAALGHVRYSTAGGRVLENIQPLLFRFSDEAIALAH 112

HSP 2 Score: 61.2326 bits (147), Expect = 3.743e-32
Identity = 30/79 (37.97%), Postives = 48/79 (60.76%), Query Frame = 3 
Query:  621 LVNYNTLRAKLEDNGSIFNTSSDTVVVLHLIPISKARPFFLRIVDVCEKLEGAYSMVFVTEDKLGAVRDPYGFNALVKG 857
            L N  +LR +LED G+IF ++SDT V++HLI     +P+  ++     ++ G ++ V +TE  L A  DP+GF  +V G
Sbjct:  116 LTNAISLRRQLEDQGAIFQSTSDTEVLMHLIRRQVGQPWLTQLKTALNEVHGGFAFVLLTEHGLYAAVDPHGFRPMVVG 194
The following BLAST results are available for this feature:
BLAST of DY303083 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
PUR1_SOYBN9.089e-7380.95Amidophosphoribosyltransferase, chloroplastic OS=G... [more]
PUR1_VIGAC3.811e-4250.48Amidophosphoribosyltransferase, chloroplastic (Fra... [more]
PUR1_STAAN5.790e-3345.63Amidophosphoribosyltransferase OS=Staphylococcus a... [more]
PUR1_STAAM5.790e-3345.63Amidophosphoribosyltransferase OS=Staphylococcus a... [more]
PUR1_STAES7.541e-3344.66Amidophosphoribosyltransferase OS=Staphylococcus e... [more]
PUR1_STAEQ7.541e-3344.66Amidophosphoribosyltransferase OS=Staphylococcus e... [more]
PUR1_STAAW2.825e-3244.66Amidophosphoribosyltransferase OS=Staphylococcus a... [more]
PUR1_STAAS2.825e-3244.66Amidophosphoribosyltransferase OS=Staphylococcus a... [more]
PUR1_STAAC2.825e-3244.66Amidophosphoribosyltransferase OS=Staphylococcus a... [more]
PUR1_LACCA3.743e-3245.63Amidophosphoribosyltransferase (Fragment) OS=Lacto... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionKN0AAQ4YM11RM1 CitNFL Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY303083 ID=DY303083; Name=DY303083; organism=Citrus clementina; type=EST; length=925bp
AAAACAAATAAAATAAAATAGAATAAATTCAGCCATTAATGGCAGCCACC
ACAACAGGCACCGCCAACATCTGCACTCTCTCAAACCCATCTCCCTCTGC
TAACCCTAAGCCGTCGCAAAAACCCCATCTTTCTCTTTTCTCCAAAACCC
TGCAAAAAAACCCTTTTTTCTCCTCAGAAACAACAATCTTTAATCCACAA
AAATAACTTCACTGTTCTATCAAAAAACCCCATCTCTGAGTTTTTCACCA
CAAATAAAGAATACCCAGATGACGGTTTTGGCAGTTTTGATGATGATGAC
GACGATAAGCCCCGTGAGGAGTGTGGTGTTGTGGGCATCTATGGTGACCC
AGAGGCCTCGCGTTTGTGCTATTTGGCGCTACACGCGATGCAGCATCGCG
GCCTAGAAGGTGCAGGTATAGTTGCTGTGAATAACAAAGTTCTTCAGTCT
ATTACTGGTGTTGGTTTAGTCTCTGATGTGTTTAATCAGTCTAAACTTGA
TCAATTGCCGGGTGATATGGCCATTGGTCATGCTAGATATTCGACTGCGG
GTAGTTCTATGCTTAAGAATGTGCAGCCTTTTGTTGCTGGGTACAGGTTT
GGTTCTGTTGGTGTTGCACATTAGTTAATTATAATACTTTGAGGGCTAAG
CTTGAAGATAATGGATCCATTTTCAATACTAGTTCTGATACTGTGGTTGT
TCTCCATTTGATTCCCATATCGAAAGCTAGGCCGTTCTTTTTGAGGATTG
TGGATGTTTGTGAGAAGCTTGAGGGTGCTTATTCAATGGTGTTTGTTACT
GAGGATAAGCTTGGTGCGGTTCGTGACCCGTATGGGTTTAATGCATTGGT
GAAGGGGAAGGAAGAGTAATGGTGCTCTGGGTTTTGCGTCTGAAACTTGT
GCAAAATTTGAATGAAGCCACTTAC
back to top