FC876785

Overview
NameFC876785
Unique NameFC876785
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length669
Libraries
Library NameType
EHRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_RAT (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 3.687e-37
Identity = 81/181 (44.75%), Postives = 115/181 (63.54%), Query Frame = 1
Query:  130 ISFTRCIKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHS-DWIGKPFGSMVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLPDSIFLDL 669
            +++   IKEGD  I+   H  M AV+V + +  Q R G  +HS D IG+PFGS V  ++GG+VY+L PTPELWT+ L HRTQILY  DI+ + M LEL PG +V               R++APTGH++T +FH+QRA  ARE+F++  VS +VTV  +D+   GF    + + D++FLD+
Sbjct:    4 VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRGGWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRSIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLDI 182          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_MOUSE (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Mus musculus GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 6.290e-37
Identity = 81/181 (44.75%), Postives = 114/181 (62.98%), Query Frame = 1
Query:  130 ISFTRCIKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHS-DWIGKPFGSMVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLPDSIFLDL 669
            +++   IKEGD  I+   H  M AV+V + +  Q R G  +HS D IG+PFGS V  ++GG+VY+L PTPELWT+ L HRTQILY  DI+ + M LEL PG +V               R+VAPTGH++T +FH+QRA  ARE+F+   +S +VTV  +D+   GF    + + D++FLD+
Sbjct:    4 VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRGGWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRSVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLDI 182          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_HUMAN (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.073e-36
Identity = 81/181 (44.75%), Postives = 110/181 (60.77%), Query Frame = 1
Query:  130 ISFTRCIKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHS-DWIGKPFGSMVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLPDSIFLDL 669
            +++   IKEGD  I+   H  M AV+V + +  Q R G  +HS D IG+PFGS V   +GG+VY+L PTPELWTL L HRTQILY  DI+ + M LEL PG +V               R +APTGH++T +FH+QRA  ARE+F+   V  +VTV  +D+   GF    +   D++FLD+
Sbjct:    4 VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVA--DAVFLDI 182          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_BOVIN (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Bos taurus GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 9.083e-36
Identity = 81/181 (44.75%), Postives = 110/181 (60.77%), Query Frame = 1
Query:  130 ISFTRCIKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHS-DWIGKPFGSMVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLPDSIFLDL 669
            +++   IKEGD  I+   H  M AV+V + +  Q R G  +HS D IG+PFGS V   +GG+VY+L PTPELWTL L HRTQILY  DI+ + M LEL PG +V               R +APTGH++T +FH+QRA  ARE+F+   V  +VTV  +D+   GF    +   D++FLD+
Sbjct:    4 VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHVA--DAVFLDI 182          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_DICDI (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum GN=trmt61a PE=1 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 6.092e-32
Identity = 73/179 (40.78%), Postives = 110/179 (61.45%), Query Frame = 1
Query:  142 RCIKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVFSNKG-GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQG-QGFPDEFIGLP-DSIFLDL 669
            + IKEGD V++Y   D M  + +  N+ + ++FG+++H + IGK +GS + S+ G GF +++A TPELW++ L HRTQIL+  DIS +I  LEL  G                 AR +AP GH++TF+FHE+R   AR+DF+  G+  ++TV  RD  G +GF  + I    D++FLDL
Sbjct:    6 KIIKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVIAMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKGHLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDL 184          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_KLULA (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Kluyveromyces lactis GN=TRM61 PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.315e-26
Identity = 64/166 (38.55%), Postives = 95/166 (57.23%), Query Frame = 1
Query:  148 IKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVF-----SNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGV--SSFVTVGVRDIQGQGF 624
            IKEGDLV+ +   D +K + V     F  R+GAF HSD IGKPFGS +      SN+ GF+++L PTPELW++ L HRTQI+Y  D S+++  +E  P   V              AR+    GH+++++FHE R   A+++F+  G+  +   T+  RD+   GF
Sbjct:   11 IKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHSDMIGKPFGSQIAIRTKGSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSFSHAFARS---AGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCKDGF 173          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_CANAL (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Candida albicans GN=TRM61 PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.825e-26
Identity = 67/176 (38.07%), Postives = 96/176 (54.55%), Query Frame = 1
Query:  148 IKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVFSNKG-GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLP-DSIFLDL 669
            I+EGDLV+ Y     +K + V +   F  R+G F+H   IG  +G  +   KG GF++LL PTPELWTL L HRTQI+Y  D S++I  L + PG  V              AR V  +G V+T++FHE R   A+++ E   + +  T+  RD+   GF  +   +  D +FLDL
Sbjct:   10 IEEGDLVLAYISRSTIKPINVKKGEIFNTRYGHFEHDKMIGMKYGEQMPGAKGYGFIHLLHPTPELWTLSLPHRTQIVYSPDSSYIIQRLNVKPGSRVIEAGTGSASFTHSFARTVTLSGKVFTYEFHEPRYLEAKKELEEHKLDN-TTITHRDVCNDGFSIDNESIEGDVVFLDL 184          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_YEAST (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Saccharomyces cerevisiae GN=GCD14 PE=1 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 6.524e-26
Identity = 66/165 (40.00%), Postives = 92/165 (55.76%), Query Frame = 1
Query:  148 IKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVF-----SNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTG-VSSFVTVGVRDIQGQGF 624
            IKEGDL +++   D +K V++     F  R+G+F H D IGKP+GS +      SNK  FV++L PTPELWTL L HRTQI+Y  D S+++  L   P   V              AR+V   GH+++F+FH  R   A E+F+  G +   VT+  RD+   GF
Sbjct:   13 IKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTKGSNKFAFVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARSV---GHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGF 174          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_CRYNE (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Cryptococcus neoformans GN=TRM61 PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.113e-25
Identity = 67/176 (38.07%), Postives = 104/176 (59.09%), Query Frame = 1
Query:  148 IKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVFSNKG--GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGFPDEFIGLPDSIFLDL 669
            I+ GD+VI+Y   D M A+ +     F N++G + H   IG+ +GS + S     G+V++L PTPELWTL L HRTQILY+ DIS++ M L +  G  V              +R+V P+G V +F++H QR  +A ++FE  G+++ V +  R++  +GF D   G+ + +FLDL
Sbjct:   14 IEAGDIVILYMARDNMTAITITPGETFHNKYGRYPHDMLIGQKYGSKIHSPPPHPGYVHVLRPTPELWTLSLPHRTQILYLPDISYITMRLGVRVGGKVIEAGTGSGSMTHSLSRSVGPSGQVMSFEYHRQRFETALKEFESHGLTN-VRLQHRNVCKEGFGDA-QGV-EGVFLDL 186          
BLAST of FC876785 vs. ExPASy Swiss-Prot
Match: TRM61_DEBHA (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Debaryomyces hansenii GN=TRM61 PE=3 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 2.741e-24
Identity = 64/184 (34.78%), Postives = 96/184 (52.17%), Query Frame = 1
Query:  148 IKEGDLVIVYERHDCMKAVKVCQNSAFQNRFGAFKHSDWIGKPFGSMVFSNKG-GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLELVPGCLVXXXXXXXXXXXXXXARAVAPTGHVYTFDFHEQRAASAREDFERTGVSSFVTVGVRDIQGQGF-----PDEFIG----LPDSIFLDL 669
            I+EGDLV+ +     +K + V + S    R+G F+H   IG  +G  +   KG GF++LL PTPELWT+ L HRTQI+Y  D S++I  L +  G  V              AR +   G ++T++FHE R   A+++ E  G+ +   +  RD+   GF     P++F        D +FLDL
Sbjct:   10 IEEGDLVLAFLGRSNIKPITVTKGSQLNTRYGNFEHDRMIGMKYGEQMPGAKGVGFIHLLYPTPELWTVSLPHRTQIVYTPDSSYIIQRLNVTSGTRVIEAGTGSGSFSHSFARTIGVEGRLFTYEFHEPRYIEAKKELEDHGLLANTVITHRDVCNDGFEISNIPEDFKKDDGICGDVVFLDL 193          
The following BLAST results are available for this feature:
BLAST of FC876785 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 16
Match NameE-valueIdentityDescription
TRM61_RAT3.687e-3744.75tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_MOUSE6.290e-3744.75tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_HUMAN1.073e-3644.75tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_BOVIN9.083e-3644.75tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_DICDI6.092e-3240.78tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_KLULA1.315e-2638.55tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_CANAL3.825e-2638.07tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_YEAST6.524e-2640.00tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_CRYNE1.113e-2538.07tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]
TRM61_DEBHA2.741e-2434.78tRNA (adenine-N(1)-)-methyltransferase catalytic s... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAN3AG05RM1 EHR Citrus clementina cDNA clone KN0AAN3AG05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876785 ID=FC876785; Name=FC876785; organism=Citrus clementina; type=EST; length=669bp
TTCTCTCTGCCTCTCAGATTTTGCTGTCTGACTTCCTGAACCCGTCACAA
TTTTATTTGCAGATAATTGCTTGGTTTTAGTAACTCTTTGATAATTTAAA
GGATGTTGCCAACTGATCCAACGAAAAAGATATCCTTTACTCGATGCATT
AAAGAAGGGGACTTGGTTATTGTCTATGAGAGGCATGACTGCATGAAAGC
TGTTAAAGTATGCCAGAACTCAGCTTTCCAGAACCGTTTTGGTGCGTTTA
AACATTCAGACTGGATTGGGAAGCCCTTTGGGTCTATGGTGTTCAGTAAC
AAGGGCGGATTTGTTTACTTGTTAGCCCCAACTCCGGAGTTATGGACTCT
GGTTTTGAGCCATAGAACTCAGATACTTTACATTGCAGATATTAGCTTTG
TCATCATGTACTTAGAACTTGTTCCTGGTTGTTTGGTTCTGGAGTCAGGG
ACTGGAAGTGGATCTTTGACAACCTCTCTTGCAAGGGCTGTAGCTCCTAC
AGGACATGTCTATACTTTTGATTTTCATGAGCAAAGGGCTGCCTCAGCTA
GGGAAGACTTTGAGAGGACAGGAGTGAGCAGTTTTGTTACTGTGGGTGTT
AGAGATATCCAGGGTCAAGGATTTCCTGATGAATTTATTGGGTTACCTGA
TTCTATATTTCTGGACCTA
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