FC878190

Overview
NameFC878190
Unique NameFC878190
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length935
Libraries
Library NameType
IVIA1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig2675 contig Ccv1_Contig2675:41..976. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_ARATH (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana GN=ECR1 PE=1 SV=2)

HSP 1 Score: 330.487 bits (846), Expect = 9.855e-95
Identity = 159/187 (85.03%), Postives = 170/187 (90.91%), Query Frame = 2 
Query:  332 GNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            GNLV P F PG  LRDD+++Y RILV+GAGGLGCELLKDLALSGF+NLEVIDMDRIEV+NLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV IVPHF RIEDK+I FYNDFNII LGLDSIEAR YIN VAC FLEY  DD P+ ETIKPMVDGGTEGFKGHARVI+PGVTPCFECTI+LFPPQVKF
Sbjct:   26 GNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHARVILPGVTPCFECTIYLFPPQVKF 212

HSP 2 Score: 38.891 bits (89), Expect = 9.855e-95
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            FPP  + PLCTLAETPR AAH
Sbjct:  206 FPPQVKFPLCTLAETPRNAAH 226
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_CAEEL (NEDD8-activating enzyme E1 catalytic subunit OS=Caenorhabditis elegans GN=uba-3 PE=2 SV=2)

HSP 1 Score: 221.09 bits (562), Expect = 1.966e-59
Identity = 113/182 (62.09%), Postives = 132/182 (72.53%), Query Frame = 2 
Query:  347 PTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            P F PG E  + LQ   +ILV+GAGGLGCELLK+LALSGF+ +EVIDMD I+VSNLNRQFLFR  DVGK KAEVAA  V +RV G  +  H CRIEDK   FY  F+II+ GLDSI AR +IN + C  +    D KP E TI PM+DGGTEGFKG+ARVI P  T C +CT+ L+PPQV F
Sbjct:   28 PWFVPGPENFEALQN-TKILVIGAGGLGCELLKNLALSGFRTIEVIDMDTIDVSNLNRQFLFRESDVGKSKAEVAAAFVQQRVVGCQVTAHNCRIEDKGQEFYRKFSIIICGLDSIPARRWINGMLCDLVLEMADGKPDENTIIPMIDGGTEGFKGNARVIYPKFTACIDCTLDLYPPQVNF 208

HSP 2 Score: 30.4166 bits (67), Expect = 1.966e-59
Identity = 11/21 (52.38%), Postives = 13/21 (61.90%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    PLCT+A TPR   H
Sbjct:  202 YPPQVNFPLCTIAHTPRLPEH 222
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_RAT (NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus GN=Uba3 PE=1 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 2.788e-58
Identity = 110/188 (58.51%), Postives = 140/188 (74.47%), Query Frame = 2 
Query:  329 AGNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            +G    P FEP  E    L +  ++LV+GAGGLGCELLK+LALSGF+ + VIDMD I+VSNLNRQFLFR +DVG+PKAEVAA+ + +RV   N+VPHF +I+D + +FY  F+IIV GLDSI AR +IN +  S L YE D      +I P++DGGTEGFKG+ARVI+PG+T C ECT+ L+PPQV F
Sbjct:   48 SGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNF 234

HSP 2 Score: 27.335 bits (59), Expect = 2.788e-58
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    P+CT+A  PR   H
Sbjct:  228 YPPQVNFPMCTIASMPRLPEH 248
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_MOUSE (NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus GN=Uba3 PE=2 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 2.788e-58
Identity = 110/188 (58.51%), Postives = 140/188 (74.47%), Query Frame = 2 
Query:  329 AGNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            +G    P FEP  E    L +  ++LV+GAGGLGCELLK+LALSGF+ + VIDMD I+VSNLNRQFLFR +DVG+PKAEVAA+ + +RV   N+VPHF +I+D + +FY  F+IIV GLDSI AR +IN +  S L YE D      +I P++DGGTEGFKG+ARVI+PG+T C ECT+ L+PPQV F
Sbjct:   48 SGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNF 234

HSP 2 Score: 27.335 bits (59), Expect = 2.788e-58
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    P+CT+A  PR   H
Sbjct:  228 YPPQVNFPMCTIASMPRLPEH 248
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_PONAB (NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii GN=UBA3 PE=2 SV=2)

HSP 1 Score: 219.935 bits (559), Expect = 3.635e-58
Identity = 109/188 (57.98%), Postives = 140/188 (74.47%), Query Frame = 2 
Query:  329 AGNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            +G    P FEP  E    L +  ++LV+GAGGLGCELLK+LALSGF+ + VIDMD I+VSNLNRQFLFR +D+G+PKAEVAA+ + +RV   N+VPHF +I+D + +FY  F+IIV GLDSI AR +IN +  S L YE D      +I P++DGGTEGFKG+ARVI+PG+T C ECT+ L+PPQV F
Sbjct:   48 SGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNF 234

HSP 2 Score: 27.335 bits (59), Expect = 3.635e-58
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    P+CT+A  PR   H
Sbjct:  228 YPPQVNFPMCTIASMPRLPEH 248
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_HUMAN (NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens GN=UBA3 PE=1 SV=2)

HSP 1 Score: 219.935 bits (559), Expect = 3.635e-58
Identity = 109/188 (57.98%), Postives = 140/188 (74.47%), Query Frame = 2 
Query:  329 AGNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            +G    P FEP  E    L +  ++LV+GAGGLGCELLK+LALSGF+ + VIDMD I+VSNLNRQFLFR +D+G+PKAEVAA+ + +RV   N+VPHF +I+D + +FY  F+IIV GLDSI AR +IN +  S L YE D      +I P++DGGTEGFKG+ARVI+PG+T C ECT+ L+PPQV F
Sbjct:   48 SGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNF 234

HSP 2 Score: 27.335 bits (59), Expect = 3.635e-58
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    P+CT+A  PR   H
Sbjct:  228 YPPQVNFPMCTIASMPRLPEH 248
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_DICDI (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum GN=uba3 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 3.636e-58
Identity = 103/191 (53.93%), Postives = 138/191 (72.25%), Query Frame = 2 
Query:  332 GNLVGPTFEPGAE----LRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            G    P+FEP  +    + + LQ   ++LV+GAGGLGCE+LK+LALSGF+N++VIDMD I++SNLNRQFLFR +DVGK KAEVAA  +  R++G N+ PH CRI+DKD  +Y  F I++ GLDSIEAR +IN +  + +          +TI P+VDGGTEGFKG ARVI+P ++ CFEC++  FPPQV +
Sbjct:   25 GPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCEILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTPHKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGGTEGFKGQARVILPKISSCFECSLDAFPPQVSY 215

HSP 2 Score: 29.6462 bits (65), Expect = 3.636e-58
Identity = 11/22 (50.00%), Postives = 13/22 (59.09%), Query Frame = 1 
Query:  868 AFPPSSEVPLCTLAETPRTAAH 933
            AFPP     +CT+A TPR   H
Sbjct:  208 AFPPQVSYAICTIANTPRVPEH 229
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_DANRE (NEDD8-activating enzyme E1 catalytic subunit OS=Danio rerio GN=uba3 PE=2 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 2.327e-57
Identity = 107/187 (57.22%), Postives = 139/187 (74.33%), Query Frame = 2 
Query:  332 GNLVGPTFEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            G    P FE   E    L +  +ILV+GAGGLGCELLKDLALSGF+++ V+DMD I+VSNLNRQFLFR +DVG+PKAEVAA  V +RV G ++VPHF +I+D D +FY  F+I+V GLDS+ AR ++N +  S L YE D      +I P++DGGTEGFKG+ARVI+PG+T C +CT+ L+PPQ+ F
Sbjct:   48 GPFTHPDFEASTESLQFLLDTCKILVIGAGGLGCELLKDLALSGFRHIHVVDMDTIDVSNLNRQFLFRPKDVGRPKAEVAADFVNDRVPGCSVVPHFKKIQDLDETFYRQFHIVVCGLDSVIARRWMNGMLLSLLIYE-DGVLDPSSIIPLIDGGTEGFKGNARVILPGMTACIDCTLELYPPQINF 233

HSP 2 Score: 27.335 bits (59), Expect = 2.327e-57
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            +PP    P+CT+A  PR   H
Sbjct:  227 YPPQINFPMCTIASMPRLPEH 247
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_DROME (NEDD8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster GN=CG13343 PE=1 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 1.943e-56
Identity = 104/180 (57.78%), Postives = 128/180 (71.11%), Query Frame = 2 
Query:  353 FEPGAELRDDLQEYARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            F   +E  + LQ   ++L++GAGGLGCELLKDLAL GF NL VIDMD IE+SNLNRQFLFR  D+G  KAE AA+ +  RV    + PHF +I+D D SFY  F+++V GLDSI AR +IN +  S L YE D      +I PM+DGGTEGFKG+ARVI+PG T C ECT+ LFPPQV +
Sbjct:   35 FAASSENLEFLQTKCQVLIIGAGGLGCELLKDLALMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQDFDESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNARVILPGFTACIECTLDLFPPQVNY 214

HSP 2 Score: 31.5722 bits (70), Expect = 1.943e-56
Identity = 12/21 (57.14%), Postives = 13/21 (61.90%), Query Frame = 1 
Query:  871 FPPSSEVPLCTLAETPRTAAH 933
            FPP    PLCT+A TPR   H
Sbjct:  208 FPPQVNYPLCTIANTPRLPEH 228
[Close]
BLAST of FC878190 vs. ExPASy Swiss-Prot
Match: UBA3_SCHPO (NEDD8-activating enzyme E1 catalytic subunit OS=Schizosaccharomyces pombe GN=uba3 PE=1 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 4.823e-45
Identity = 82/166 (49.40%), Postives = 121/166 (72.89%), Query Frame = 2 
Query:  395 ARILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSNLNRQFLFRMEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDISFYNDFNIIVLGLDSIEARSYINAVACSFLEYETDDKPREETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWLFPPQVKF 892
            ++IL++GAGGLGCE+LKDLALSGF++L VIDMD I+++NLNRQFLF   ++ +PKA VAA  +M+R+    + P + +I+DK I FY +F +I+ GLDS+EAR +IN+   +    +T D      + P+VDGG+EG KG ARVIIP +T C+EC++ +  P++ +
Sbjct:   45 SKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNIDEPKANVAASMIMKRIPSTVVTPFYGKIQDKTIEFYKEFKLIICGLDSVEARRWINSTLVAIA--KTGD------LIPLVDGGSEGLKGQARVIIPTITSCYECSLDMLTPKISY 202

HSP 2 Score: 26.5646 bits (57), Expect = 4.823e-45
Identity = 10/19 (52.63%), Postives = 11/19 (57.89%), Query Frame = 1 
Query:  877 PSSEVPLCTLAETPRTAAH 933
            P    P+CTLA TPR   H
Sbjct:  198 PKISYPICTLANTPRLPEH 216
The following BLAST results are available for this feature:
BLAST of FC878190 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
UBA3_ARATH9.855e-9585.03NEDD8-activating enzyme E1 catalytic subunit OS=Ar... [more]
UBA3_CAEEL1.966e-5962.09NEDD8-activating enzyme E1 catalytic subunit OS=Ca... [more]
UBA3_RAT2.788e-5858.51NEDD8-activating enzyme E1 catalytic subunit OS=Ra... [more]
UBA3_MOUSE2.788e-5858.51NEDD8-activating enzyme E1 catalytic subunit OS=Mu... [more]
UBA3_PONAB3.635e-5857.98NEDD8-activating enzyme E1 catalytic subunit OS=Po... [more]
UBA3_HUMAN3.635e-5857.98NEDD8-activating enzyme E1 catalytic subunit OS=Ho... [more]
UBA3_DICDI3.636e-5853.93NEDD8-activating enzyme E1 catalytic subunit OS=Di... [more]
UBA3_DANRE2.327e-5757.22NEDD8-activating enzyme E1 catalytic subunit OS=Da... [more]
UBA3_DROME1.943e-5657.78NEDD8-activating enzyme E1 catalytic subunit OS=Dr... [more]
UBA3_SCHPO4.823e-4549.40NEDD8-activating enzyme E1 catalytic subunit OS=Sc... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA6DF04RM1 IVIA1 Citrus clementina cDNA clone IC0AAA6DF04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC878190 ID=FC878190; Name=FC878190; organism=Citrus clementina; type=EST; length=935bp
ATCACTTCAAAGCTACTTTGGTTCATAAAGCATATGATTATTCTGCGACG
CTCCCAAATTCTGGGTAAAATCTCAAACCCATGAGCCCGTTTTCCGGTCC
ATACTCAAAACTAAAATTACAAAAAAGAGCAAAGAAGGCGGAGGTTTAAA
AGCTTCACAGCTGACACTCCCCACTCACAGACCCAGCGTCAAAGCCAAGC
TATCAGCATATTTTCGCAACGGCTAAATCAGAACCAAATACAGTGATAGT
AAGCGAGTGTGAGAATTGTTGCAAATGGCTGACACGGCACCGAGCCGATC
CCGAGACCTAGACAAGCTCTTACTCCGAGCCGGCAATCTAGTGGGCCCCA
CTTTCGAACCCGGCGCAGAATTGAGAGATGACTTACAAGAATATGCGCGA
ATTTTGGTTGTTGGAGCCGGTGGATTGGGCTGCGAATTGTTGAAAGACTT
AGCTTTATCGGGTTTTAAAAACCTAGAGGTCATTGATATGGACCGTATCG
AAGTTTCGAATCTCAATCGCCAGTTTCTTTTCAGGATGGAAGATGTTGGT
AAGCCAAAGGCAGAGGTGGCAGCAAAGCGTGTCATGGAGAGAGTTAGTGG
TGTAAATATTGTCCCACATTTTTGCCGAATTGAGGATAAAGACATTTCAT
TTTACAATGATTTTAATATCATTGTCCTTGGTCTCGATTCCATCGAGGCT
CGGAGTTATATAAATGCTGTAGCATGTAGTTTTCTTGAATATGAGACTGA
TGACAAGCCACGAGAAGAAACAATCAAACCCATGGTAGATGGGGGTACTG
AAGGTTTTAAGGGTCATGCAAGGGTTATTATACCAGGAGTCACACCGTGC
TTTGAGTGTACCATCTGGCTTTTCCCCCCTCAAGTGAAGTTCCTTTATGC
ACTCTTGCGGAAACCCCTAGAACAGCTGCTCATTG
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