FC920340

Overview
NameFC920340
Unique NameFC920340
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length714
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:44..758. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 4.171e-98
Identity = 169/205 (82.44%), Postives = 189/205 (92.20%), Query Frame = 2 
Query:   92 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            WPKKDKRRFLH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVELTYNYGV+SYDIGTGFGHFAI+T+DV K+VE +RAKGGNVTREPGPVKGG + IAFVKDPDGY FELIQRGPTPEPLCQVMLR GDL R++KF EKALGM+LLR +++PEY  T+ M+GYAEE ++ VLELTYNYGVTEYT+G
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKG 213

HSP 2 Score: 98.5969 bits (244), Expect = 3.492e-20
Identity = 52/115 (45.22%), Postives = 68/115 (59.13%), Query Frame = 2 
Query:  134 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR----AKGGNVTREPGPVKGGTTHIAFVKDPDGY 466
            RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +   AI T+DVYK  E ++      GG +TRE GP+ G  T I    DPDG+
Sbjct:  154 RVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 353.599 bits (906), Expect = 6.023e-97
Identity = 168/206 (81.55%), Postives = 185/206 (89.81%), Query Frame = 2 
Query:   92 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGG-NVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            WPKKDK+R LHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +ELTYNYGV  YDIG GFGHFAIATEDVYKL E I++     +TREPGPVKGG+T IAF +DPDGY+FELIQRGPTPEPLCQVMLR GDL RSIKFYEKALGMKLLR  D P+YKYT+AMLGYA+ED+TTV+ELTYNYGVTEYT+G
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKG 222

HSP 2 Score: 124.79 bits (312), Expect = 4.550e-28
Identity = 63/120 (52.50%), Postives = 78/120 (65.00%), Query Frame = 2 
Query:  134 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENI----RAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELI 481
            RVGDLDR+IKFY +  GMKLLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +   AI TEDVYK  E +    +  GG + R+PGP+ G  T IA   DPDG+   L+
Sbjct:  162 RVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 4.687e-41
Identity = 78/124 (62.90%), Postives = 95/124 (76.61%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH AI  +D+Y   + I+A GGNVTRE GPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 83.1889 bits (204), Expect = 1.518e-15
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            +   MLR GDL +SIKFY + +GM+LLRT +  EY+YTLA +GY +E Q  V+ELTYN+G TEY  G
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLG 72
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 164.081 bits (414), Expect = 6.768e-40
Identity = 76/124 (61.29%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH AI  +D+Y   + I+A GG VTREPGPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 82.4185 bits (202), Expect = 2.590e-15
Identity = 37/67 (55.22%), Postives = 48/67 (71.64%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            +   MLR GDL +SI+FY + +GM LLR  +  EYKYTLA LGY +E Q  V+ELTYN+GV +Y +G
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKG 72
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.335e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 85.8853 bits (211), Expect = 2.342e-16
Identity = 41/67 (61.19%), Postives = 48/67 (71.64%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELG 69
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.335e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 85.8853 bits (211), Expect = 2.342e-16
Identity = 41/67 (61.19%), Postives = 48/67 (71.64%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELG 69
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.335e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 85.8853 bits (211), Expect = 2.342e-16
Identity = 41/67 (61.19%), Postives = 48/67 (71.64%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELG 69
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.411e-37
Identity = 74/124 (59.68%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.9593 bits (206), Expect = 8.901e-16
Identity = 40/67 (59.70%), Postives = 46/67 (68.66%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   MLR GDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y  G
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMG 69
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.411e-37
Identity = 74/124 (59.68%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:  113 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.9593 bits (206), Expect = 8.901e-16
Identity = 40/67 (59.70%), Postives = 46/67 (68.66%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   MLR GDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y  G
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMG 69
[Close]
BLAST of FC920340 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.560e-36
Identity = 68/122 (55.74%), Postives = 90/122 (73.77%), Query Frame = 2 
Query:  119 LHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 484
            LH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+ELT+N+G   YD+G GFGH A+  ED+Y   + IR KGG V REPGP+K GTT IAFV+DPDGY  ELIQ
Sbjct:    4 LHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125

HSP 2 Score: 67.781 bits (164), Expect = 6.601e-11
Identity = 31/67 (46.27%), Postives = 44/67 (65.67%), Query Frame = 2 
Query:  506 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTRG 706
            L   M+R GDL +S++FY   LGM LLR  D P  ++TLA +GY +E +  V+ELT+N+G  +Y  G
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLG 69
The following BLAST results are available for this feature:
BLAST of FC920340 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_BRAOG4.171e-9882.44Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_ORYSJ6.023e-9781.55Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_VIBPA4.687e-4162.90Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH6.768e-4061.29Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL6.335e-3859.68Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI6.335e-3859.68Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO576.335e-3859.68Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY1.411e-3759.68Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI1.411e-3759.68Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY31.560e-3655.74Probable lactoylglutathione lyase OS=Synechocystis... [more]

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Properties
Property NameValue
Genbank descriptionC32007A10EF RVDevelop1 Citrus clementina cDNA clone C32007A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920340 ID=FC920340; Name=FC920340; organism=Citrus clementina; type=EST; length=714bp
GACACTTGCGACTTCACTCCGACTTTCTGAGTGATTCCATATCAAAATGG
CTGAGGCTTCTCCTGCTGCTGCTAATGCCGAGTTGCTGGAATGGCCAAAG
AAAGATAAGCGACGCTTCCTTCATGCTGTGTATCGTGTTGGTGATCTTGA
TCGCACCATCAAATTTTACACTGAATGCTTTGGGATGAAGCTGTTGAGAA
AGAGAGACGTCCCAGAAGAGAAATACTCAAATGCCTTTCTTGGATTTGGC
CCCGAACAGTCTCACTTTGTTGTGGAGTTAACATACAACTATGGTGTAAC
TTCATATGATATTGGAACTGGTTTTGGCCATTTTGCAATTGCAACTGAAG
ATGTTTACAAACTGGTTGAAAACATTCGTGCCAAGGGTGGCAATGTCACT
AGAGAGCCTGGTCCAGTTAAAGGTGGAACAACTCACATTGCCTTTGTGAA
GGATCCCGATGGTTATATTTTTGAACTCATCCAGAGAGGTCCAACTCCTG
AACCACTCTGTCAAGTAATGCTTCGCGCTGGCGATCTTGGTCGCTCCATC
AAGTTCTATGAAAAGGCTTTGGGGATGAAACTGTTAAGGACAGTTGATAA
ACCTGAATACAAGTACACCCTGGCCATGCTTGGATATGCTGAGGAAGATC
AGACTACTGTTCTGGAGTTAACTTATAATTATGGTGTGACTGAATATACC
AGGGGAATGCATAT
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