FC925146

Overview
NameFC925146
Unique NameFC925146
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length544
Libraries
Library NameType
TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC925146_ssr55 microsatellite FC925146_ssr55:55..70. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig1951 contig Ccv1_Contig1951:2..545. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: Y1500_ARATH (Probable tyrosine-protein phosphatase At1g05000 OS=Arabidopsis thaliana GN=At1g05000 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 8.119e-44
Identity = 58/73 (79.45%), Postives = 69/73 (94.52%), Query Frame = 2 
Query:  212 GEEL-FVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGIDGCK 427
            GEEL  +PP NF++VDNGIFRSGFPDSANFSFL++LGLRS+IYLCPEPYP+SNL+FLK+NGI+LFQFGI+G K
Sbjct:   48 GEELHLIPPLNFSMVDNGIFRSGFPDSANFSFLQTLGLRSIIYLCPEPYPESNLQFLKSNGIRLFQFGIEGNK 120

HSP 2 Score: 72.4034 bits (176), Expect = 8.119e-44
Identity = 29/31 (93.55%), Postives = 31/31 (100.00%), Query Frame = 1 
Query:  427 DERNHPLLIHCKRGKHRTGCLVGCLRKLQKW 519
            DE+NHP+LIHCKRGKHRTGCLVGCLRKLQKW
Sbjct:  140 DEKNHPVLIHCKRGKHRTGCLVGCLRKLQKW 170
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: SIW14_YEAST (Tyrosine-protein phosphatase SIW14 OS=Saccharomyces cerevisiae GN=SIW14 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 9.697e-24
Identity = 35/68 (51.47%), Postives = 45/68 (66.18%), Query Frame = 2 
Query:  227 VPPFNFAIVDNGIFRSGFPDSANFSFL-KSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGIDGCK 427
            +PP NF+ V   I+RS FP   NFSFL + L L+S++ L PE YP  NL FLK  GI+L+Q G+ G K
Sbjct:  117 IPPENFSHVVGEIYRSSFPRQENFSFLHERLKLKSILVLIPEEYPQENLNFLKLTGIKLYQVGMSGNK 184

HSP 2 Score: 62.003 bits (149), Expect = 9.697e-24
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:  436 NHPLLIHCKRGKHRTGCLVGCLRKLQKWSPVFYF 537
            N P+LIHC RGKHRTGCL+GC+RKLQ WS    F
Sbjct:  207 NQPILIHCNRGKHRTGCLIGCIRKLQNWSLTMIF 240
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_USTMA (Putative tyrosine-protein phosphatase OCA1 OS=Ustilago maydis GN=OCA1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.745e-16
Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 2 
Query:  221 LFVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGI 415
            + VPP N+ +V+   +RSG PD  NF FL+ LGL+SVI+L PE      L+F     I+L   G+
Sbjct:    1 MLVPPPNYGMVEENFYRSGQPDQLNFPFLEKLGLKSVIWLAPEEPEPGFLDFCVDQNIELHHLGV 65

HSP 2 Score: 45.4394 bits (106), Expect = 5.745e-16
Identity = 16/28 (57.14%), Postives = 23/28 (82.14%), Query Frame = 1 
Query:  439 HPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            +P+L+ C  G+HRTG +VGC RKLQ+W+
Sbjct:   93 YPVLVMCNLGRHRTGTVVGCFRKLQRWN 120
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: D1060_DICDI (Probable tyrosine-protein phosphatase DG1060 OS=Dictyostelium discoideum GN=DG1060 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 6.062e-15
Identity = 23/64 (35.94%), Postives = 38/64 (59.38%), Query Frame = 2 
Query:  227 VPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGID 418
            +PP NF +V + ++RSG P+  NF FL+ L L+ +I+L P+        F++   I+L   G+D
Sbjct:    5 IPPLNFGMVADDLYRSGQPNELNFPFLEKLQLKKIIFLAPDDPSQQFQNFVEDQDIELIHLGMD 68

HSP 2 Score: 48.1358 bits (113), Expect = 6.062e-15
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 1 
Query:  436 NHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            N+PL I C  G+HRTG +VGCLRKLQ+W+
Sbjct:   94 NYPLHIMCNLGRHRTGTVVGCLRKLQRWN 122
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_CANGA (Putative tyrosine-protein phosphatase OCA1 OS=Candida glabrata GN=OCA1 PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.067e-13
Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:  215 EELFVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQL 400
            +E  VPP NF  V+  ++RSG P   NF FL +L L+++++L  E   DS LEF   + I L
Sbjct:   43 QERIVPPLNFCPVERYLYRSGQPSPVNFPFLLNLNLKTIVWLANEEPQDSLLEFCDTHKINL 104

HSP 2 Score: 44.2838 bits (103), Expect = 1.067e-13
Identity = 15/31 (48.39%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  430 ERNHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            + N+PLL+ C  G+HRTG ++GCLR++  W+
Sbjct:  138 KENYPLLVCCGMGRHRTGTVIGCLRRIMGWN 168
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: YNF3_SCHPO (Probable tyrosine-protein phosphatase C17A3.03c OS=Schizosaccharomyces pombe GN=SPBC17A3.03c PE=1 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 1.780e-13
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  427 DERNHPLLIHCKRGKHRTGCLVGCLRKLQKW 519
            ++ N P+L+HC RGKHRTG ++GCLR L  W
Sbjct:  187 NKENWPVLLHCSRGKHRTGIVIGCLRALMNW 217

HSP 2 Score: 46.595 bits (109), Expect = 1.780e-13
Identity = 24/66 (36.36%), Postives = 39/66 (59.09%), Query Frame = 2 
Query:  233 PFNFAIVDNGI-FRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGIDGCK 427
            P NF +V  GI +RS  P ++NF+FL+SL +R++I L  E Y + +L +   + I  +   + G K
Sbjct:   91 PDNFGVVYPGIIYRSACPRASNFNFLESLHIRTIISLRQEEYSEEDLHYFTKHHINYYHIAMPGSK 156
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_KLULA (Putative tyrosine-protein phosphatase OCA1 OS=Kluyveromyces lactis GN=OCA1 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.802e-13
Identity = 27/61 (44.26%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:  218 ELFVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQL 400
            E  VPP NF  V+  ++RSG P + NF FL +L L+++I+L  E   D+ LEF   + IQL
Sbjct:   37 ERIVPPLNFCPVERYLYRSGQPSNVNFPFLLNLKLKTIIWLANEEPQDALLEFCDDHNIQL 97

HSP 2 Score: 43.1282 bits (100), Expect = 1.802e-13
Identity = 15/32 (46.88%), Postives = 24/32 (75.00%), Query Frame = 1 
Query:  427 DERNHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            +  ++PLL+ C  G+HRTG ++GCLR+L  W+
Sbjct:  130 NSESYPLLVCCGMGRHRTGTVIGCLRRLMGWN 161
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_PICST (Putative tyrosine-protein phosphatase OCA1 OS=Pichia stipitis GN=OCA1 PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 1.802e-13
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 2 
Query:  227 VPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQLFQFGI 415
            +PP NF  V+  ++RSG P + N SFL+ L L+++++L  E   D  LEF   + I +   GI
Sbjct:   36 IPPLNFCPVERQLYRSGQPSAINQSFLEQLNLKTILWLASEEPQDDFLEFSNDHNINIEFVGI 98

HSP 2 Score: 46.9802 bits (110), Expect = 1.802e-13
Identity = 17/32 (53.12%), Postives = 25/32 (78.12%), Query Frame = 1 
Query:  427 DERNHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            +  N+PLL+ C  G+HRTG ++GCLR+LQ W+
Sbjct:  136 NRENYPLLVCCGMGRHRTGTVIGCLRRLQGWN 167
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_YEAST (Putative tyrosine-protein phosphatase OCA1 OS=Saccharomyces cerevisiae GN=OCA1 PE=1 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 3.026e-13
Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:  215 EELFVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQL 400
            +E  VPP NF  V+  ++RSG P   NF FL +L L+++I+L  E   D+ LEF   + I L
Sbjct:   64 QERIVPPLNFCPVERYLYRSGQPSPVNFPFLLNLKLKTIIWLSNEEPQDTLLEFCDTHRINL 125

HSP 2 Score: 44.669 bits (104), Expect = 3.026e-13
Identity = 15/31 (48.39%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  430 ERNHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            + N+PLL+ C  G+HRTG ++GCLR++  W+
Sbjct:  159 QENYPLLVCCGMGRHRTGTVIGCLRRIMGWN 189
[Close]
BLAST of FC925146 vs. ExPASy Swiss-Prot
Match: OCA1_YEAS7 (Putative tyrosine-protein phosphatase OCA1 OS=Saccharomyces cerevisiae (strain YJM789) GN=OCA1 PE=3 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 3.026e-13
Identity = 26/62 (41.94%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:  215 EELFVPPFNFAIVDNGIFRSGFPDSANFSFLKSLGLRSVIYLCPEPYPDSNLEFLKANGIQL 400
            +E  VPP NF  V+  ++RSG P   NF FL +L L+++I+L  E   D+ LEF   + I L
Sbjct:   64 QERIVPPLNFCPVERYLYRSGQPSPVNFPFLLNLKLKTIIWLSNEEPQDTLLEFCDTHRINL 125

HSP 2 Score: 44.669 bits (104), Expect = 3.026e-13
Identity = 15/31 (48.39%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  430 ERNHPLLIHCKRGKHRTGCLVGCLRKLQKWS 522
            + N+PLL+ C  G+HRTG ++GCLR++  W+
Sbjct:  159 QENYPLLVCCGMGRHRTGTVIGCLRRIMGWN 189
The following BLAST results are available for this feature:
BLAST of FC925146 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 15
Match NameE-valueIdentityDescription
Y1500_ARATH8.119e-4479.45Probable tyrosine-protein phosphatase At1g05000 OS... [more]
SIW14_YEAST9.697e-2451.47Tyrosine-protein phosphatase SIW14 OS=Saccharomyce... [more]
OCA1_USTMA5.745e-1643.08Putative tyrosine-protein phosphatase OCA1 OS=Usti... [more]
D1060_DICDI6.062e-1535.94Probable tyrosine-protein phosphatase DG1060 OS=Di... [more]
OCA1_CANGA1.067e-1341.94Putative tyrosine-protein phosphatase OCA1 OS=Cand... [more]
YNF3_SCHPO1.780e-1358.06Probable tyrosine-protein phosphatase C17A3.03c OS... [more]
OCA1_KLULA1.802e-1344.26Putative tyrosine-protein phosphatase OCA1 OS=Kluy... [more]
OCA1_PICST1.802e-1338.10Putative tyrosine-protein phosphatase OCA1 OS=Pich... [more]
OCA1_YEAST3.026e-1341.94Putative tyrosine-protein phosphatase OCA1 OS=Sacc... [more]
OCA1_YEAS73.026e-1341.94Putative tyrosine-protein phosphatase OCA1 OS=Sacc... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionKN0AAP3YC20FM1 TF Citrus clementina cDNA clone KN0AAP3YC20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC925146 ID=FC925146; Name=FC925146; organism=Citrus clementina; type=EST; length=544bp
GAGATTCTTTTATTTCAATATAATACTGAATCTCTTATAAAACTATATTT
ATACATATATATATATATATTAATTTGGAATCCAATTGCTACTTTTTTTT
ATTTTTGGCGGGAAATAAATGAAAGTCAACGGCTGTGATGGAGAGATTCG
CACGGTAAGGGAGAAATCGCCGGCTCCGATAAATGGAACAGGCGAGAATG
GCTCAAGAGACGGTGAAGAGCTGTTTGTTCCGCCGTTTAACTTCGCGATT
GTCGATAACGGCATCTTCCGGTCAGGCTTTCCTGATTCTGCCAATTTCAG
CTTCTTGAAGTCTTTAGGGCTCCGTTCTGTCATATATTTGTGCCCCGAAC
CTTATCCAGACTCGAATTTAGAGTTCTTGAAGGCTAATGGGATTCAGCTT
TTTCAGTTTGGAATCGATGGATGCAAGATGAAAGGAACCACCCGCTTCTA
ATTCATTGCAAACGAGGAAAGCACCGTACTGGTTGTCTTGTGGGCTGCTT
GAGGAAATTGCAAAAATGGTCGCCTGTCTTCTATTTTTGATGAC
back to top