FC925764

Overview
NameFC925764
Unique NameFC925764
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length619
Libraries
Library NameType
TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCE1_ARATH (Glutamate decarboxylase 1 OS=Arabidopsis thaliana GN=GAD1 PE=1 SV=2)

HSP 1 Score: 362.844 bits (930), Expect = 1.778e-104
Identity = 175/188 (93.09%), Postives = 183/188 (97.34%), Query Frame = 1 
Query:    1 PECDHLMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            PECD L+M++INKNYVDMDEYPVTTELQNRCVN+IA LFNAPLE++ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKP DKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDP +AV+MVDENTICVAAILGSTLNGEFEDVKLLNDLL EKNKETGWDTPIHVDAASGGFIAPFLYP
Sbjct:   70 PECDKLIMSSINKNYVDMDEYPVTTELQNRCVNMIAHLFNAPLEEAETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPVDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPQQAVDMVDENTICVAAILGSTLNGEFEDVKLLNDLLVEKNKETGWDTPIHVDAASGGFIAPFLYP 257

HSP 2 Score: 36.965 bits (84), Expect = 1.778e-104
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  PELEWDFRLPLVKSI
Sbjct:  254 FLYPELEWDFRLPLVKSI 271
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCE_PETHY (Glutamate decarboxylase OS=Petunia hybrida GN=GAD PE=1 SV=1)

HSP 1 Score: 360.147 bits (923), Expect = 1.144e-103
Identity = 175/188 (93.09%), Postives = 179/188 (95.21%), Query Frame = 1 
Query:    1 PECDHLMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            PECD LMM +INKNYVDMDEYPVTTELQNRCVN+IA LFNAPLED ETAVGVGTVGSSEAIMLAGLAFKRKWQNK KA+GKP DKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDP KAVEMVDENTICVAAILGSTLNGEFEDVK LNDLL EKNKETGWDTPIHVDAASGGFIAPF+YP
Sbjct:   70 PECDKLMMDSINKNYVDMDEYPVTTELQNRCVNMIAHLFNAPLEDGETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAQGKPCDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPEKAVEMVDENTICVAAILGSTLNGEFEDVKRLNDLLVEKNKETGWDTPIHVDAASGGFIAPFIYP 257

HSP 2 Score: 36.965 bits (84), Expect = 1.144e-103
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  PELEWDFRLPLVKSI
Sbjct:  254 FIYPELEWDFRLPLVKSI 271
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCE2_ARATH (Glutamate decarboxylase 2 OS=Arabidopsis thaliana GN=GAD2 PE=1 SV=1)

HSP 1 Score: 358.221 bits (918), Expect = 4.326e-103
Identity = 173/188 (92.02%), Postives = 181/188 (96.28%), Query Frame = 1 
Query:    1 PECDHLMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            PECD L+M +INKNYVDMDEYPVTTELQNRCVNIIARLFNAPLE+SETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKP+DKPNIVTGANVQVCWEKFARYFEVELKEV LSEGYYVMDP KA EMVDENTICVAAILGSTLNGEFEDVK LNDLL +KN+ETGW+TPIHVDAASGGFIAPF+YP
Sbjct:   69 PECDKLIMDSINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEESETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFEVELKEVNLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDVKRLNDLLVKKNEETGWNTPIHVDAASGGFIAPFIYP 256

HSP 2 Score: 36.965 bits (84), Expect = 4.326e-103
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  PELEWDFRLPLVKSI
Sbjct:  253 FIYPELEWDFRLPLVKSI 270
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCE_SOLLC (Glutamate decarboxylase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 352.829 bits (904), Expect = 3.977e-101
Identity = 169/188 (89.89%), Postives = 182/188 (96.81%), Query Frame = 1 
Query:    1 PECDHLMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            PECD L+M++INKNYVDMDEYPVTTELQNRCVN++A LF+AP+ D ETAVGVGTVGSSEAIMLAGLAFKRKWQ+KRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKL EGYYVMDPAKAVE+VDENTICVAAILGSTL GEFEDVKLLN+LLT+KNKETGW+TPIHVDAASGGFIAPFL+P
Sbjct:   71 PECDKLIMSSINKNYVDMDEYPVTTELQNRCVNMLAHLFHAPVGDDETAVGVGTVGSSEAIMLAGLAFKRKWQSKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLKEGYYVMDPAKAVEIVDENTICVAAILGSTLTGEFEDVKLLNELLTKKNKETGWETPIHVDAASGGFIAPFLWP 258

HSP 2 Score: 35.8094 bits (81), Expect = 3.977e-101
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  P+LEWDFRLPLVKSI
Sbjct:  255 FLWPDLEWDFRLPLVKSI 272
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: GADA_DICDI (Glutamate decarboxylase A OS=Dictyostelium discoideum GN=gadA PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 5.092e-56
Identity = 103/183 (56.28%), Postives = 133/183 (72.68%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            LM   I+KN +D DEYP T E+++RCV+I+A L+N+P E SET +G  T GSSEA ML G+A K +W+  RK +GKPFDKPNIVTG  VQ+CW KFA YF++EL+EV +    Y+M P +A++  DENTI V   LG T   ++EDVK +++ L +  KETG D PIHVDAASGGF+APFL P
Sbjct:   75 LMDKCIDKNMIDKDEYPQTAEIESRCVHILADLWNSP-ESSET-IGCSTTGSSEAAMLGGMALKWRWRENRKKQGKPFDKPNIVTGP-VQICWHKFALYFDIELREVPMEHDRYIMTPEEAIKRCDENTIGVIPTLGVTFTLQYEDVKGISEALDKFEKETGLDIPIHVDAASGGFVAPFLQP 254

HSP 2 Score: 31.5722 bits (70), Expect = 5.092e-56
Identity = 13/18 (72.22%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  PE+ WDFRLP VKSI
Sbjct:  251 FLQPEIVWDFRLPRVKSI 268
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: GADB_DICDI (Glutamate decarboxylase B OS=Dictyostelium discoideum GN=gadB PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 3.791e-50
Identity = 93/181 (51.38%), Postives = 127/181 (70.17%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFL 558
            +M   I+KN +D DEYP + E++NRC++I+A L+NAP  DS   +G  T GSSEA ML GLA K  W+  RK  G P+DKPNIVTG  VQ+CW KFA YF++E++E+ +  G YVM+  + ++ VDENTI V   LG T   ++EDV  +++ L +  KE+G + PIHVDAASGGF+APF+
Sbjct:   73 IMDKCIDKNMIDKDEYPQSAEIENRCLHILADLWNAP--DSSDTIGCSTTGSSEAAMLGGLALKWNWRENRKKLGLPYDKPNIVTGP-VQICWHKFALYFDIEIREIPMENGRYVMNSEEVLKRVDENTIGVIPTLGVTFTLQYEDVFSISNALDKFEKESGINIPIHVDAASGGFVAPFI 250

HSP 2 Score: 28.1054 bits (61), Expect = 3.791e-50
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F   E+ WDFRLP VKSI
Sbjct:  249 FIQQEIIWDFRLPRVKSI 266
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCEB_SHIFL (Glutamate decarboxylase beta OS=Shigella flexneri GN=gadB PE=2 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 1.859e-49
Identity = 89/183 (48.63%), Postives = 124/183 (67.76%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            LM  +INKN++D +EYP +  +  RCVN++A L++AP   +  AVG  T+GSSEA ML G+A K +W+ + +A GKP DKPN+V G  VQ+CW KFARY++VEL+E+ +  G   MDP + +E  DENTI V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P
Sbjct:   75 LMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGP-VQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAP 256

HSP 2 Score: 30.8018 bits (68), Expect = 1.859e-49
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  P++ WDFRLP VKSI
Sbjct:  253 FVAPDIVWDFRLPRVKSI 270
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCEB_ECOLI (Glutamate decarboxylase beta OS=Escherichia coli (strain K12) GN=gadB PE=1 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 1.859e-49
Identity = 89/183 (48.63%), Postives = 124/183 (67.76%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            LM  +INKN++D +EYP +  +  RCVN++A L++AP   +  AVG  T+GSSEA ML G+A K +W+ + +A GKP DKPN+V G  VQ+CW KFARY++VEL+E+ +  G   MDP + +E  DENTI V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P
Sbjct:   75 LMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGP-VQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAP 256

HSP 2 Score: 30.8018 bits (68), Expect = 1.859e-49
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  P++ WDFRLP VKSI
Sbjct:  253 FVAPDIVWDFRLPRVKSI 270
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCEB_ECO57 (Glutamate decarboxylase beta OS=Escherichia coli O157:H7 GN=gadB PE=2 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 1.859e-49
Identity = 89/183 (48.63%), Postives = 124/183 (67.76%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            LM  +INKN++D +EYP +  +  RCVN++A L++AP   +  AVG  T+GSSEA ML G+A K +W+ + +A GKP DKPN+V G  VQ+CW KFARY++VEL+E+ +  G   MDP + +E  DENTI V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P
Sbjct:   75 LMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGP-VQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAP 256

HSP 2 Score: 30.8018 bits (68), Expect = 1.859e-49
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  P++ WDFRLP VKSI
Sbjct:  253 FVAPDIVWDFRLPRVKSI 270
[Close]
BLAST of FC925764 vs. ExPASy Swiss-Prot
Match: DCEA_SHIFL (Glutamate decarboxylase alpha OS=Shigella flexneri GN=gadA PE=2 SV=2)

HSP 1 Score: 185.267 bits (469), Expect = 1.859e-49
Identity = 89/183 (48.63%), Postives = 124/183 (67.76%), Query Frame = 1 
Query:   16 LMMAAINKNYVDMDEYPVTTELQNRCVNIIARLFNAPLEDSETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLLNDLLTEKNKETGWDTPIHVDAASGGFIAPFLYP 564
            LM  +INKN++D +EYP +  +  RCVN++A L++AP   +  AVG  T+GSSEA ML G+A K +W+ + +A GKP DKPN+V G  VQ+CW KFARY++VEL+E+ +  G   MDP + +E  DENTI V    G T  G +E  + L+D L +   +TG D  +H+DAASGGF+APF+ P
Sbjct:   75 LMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGP-VQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAP 256

HSP 2 Score: 30.8018 bits (68), Expect = 1.859e-49
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  554 FCTPELEWDFRLPLVKSI 607
            F  P++ WDFRLP VKSI
Sbjct:  253 FVAPDIVWDFRLPRVKSI 270
The following BLAST results are available for this feature:
BLAST of FC925764 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
DCE1_ARATH1.778e-10493.09Glutamate decarboxylase 1 OS=Arabidopsis thaliana ... [more]
DCE_PETHY1.144e-10393.09Glutamate decarboxylase OS=Petunia hybrida GN=GAD ... [more]
DCE2_ARATH4.326e-10392.02Glutamate decarboxylase 2 OS=Arabidopsis thaliana ... [more]
DCE_SOLLC3.977e-10189.89Glutamate decarboxylase OS=Solanum lycopersicum PE... [more]
GADA_DICDI5.092e-5656.28Glutamate decarboxylase A OS=Dictyostelium discoid... [more]
GADB_DICDI3.791e-5051.38Glutamate decarboxylase B OS=Dictyostelium discoid... [more]
DCEB_SHIFL1.859e-4948.63Glutamate decarboxylase beta OS=Shigella flexneri ... [more]
DCEB_ECOLI1.859e-4948.63Glutamate decarboxylase beta OS=Escherichia coli (... [more]
DCEB_ECO571.859e-4948.63Glutamate decarboxylase beta OS=Escherichia coli O... [more]
DCEA_SHIFL1.859e-4948.63Glutamate decarboxylase alpha OS=Shigella flexneri... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionKN0AAP8YP02FM1 TF Citrus clementina cDNA clone KN0AAP8YP02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC925764 ID=FC925764; Name=FC925764; organism=Citrus clementina; type=EST; length=619bp
CCTGAATGTGACCATCTTATGATGGCTGCCATTAACAAGAACTACGTTGA
CATGGATGAGTATCCTGTCACCACCGAGCTTCAGAATCGATGTGTGAACA
TTATAGCCCGACTGTTCAATGCTCCACTGGAGGACTCCGAGACGGCTGTC
GGAGTTGGAACCGTAGGATCATCGGAAGCCATAATGTTGGCCGGCCTTGC
ATTCAAAAGAAAGTGGCAGAACAAGCGCAAGGCTGAAGGAAAGCCCTTTG
ACAAACCCAACATTGTCACTGGAGCGAACGTTCAGGTCTGCTGGGAGAAA
TTTGCAAGGTATTTTGAGGTGGAGTTGAAGGAAGTGAAGCTGTCGGAAGG
CTACTACGTGATGGACCCAGCCAAAGCTGTAGAAATGGTGGATGAAAACA
CTATCTGTGTTGCTGCTATTCTGGGTTCTACTCTCAATGGAGAATTTGAA
GATGTCAAGCTCTTGAACGACCTTTTGACAGAGAAGAACAAGGAAACAGG
ATGGGATACTCCAATTCATGTTGATGCTGCTAGTGGTGGCTTCATTGCAC
CATTTTTGTACCCCAGAGCTTGAATGGGACTTCCGCTTGCCGCTGGTGAA
GAGTATTTAATGTTAGTGG
back to top