FC932475

Overview
NameFC932475
Unique NameFC932475
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length702
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_ARATH (Phosphoglycerate kinase, chloroplastic OS=Arabidopsis thaliana GN=At1g56190 PE=1 SV=2)

HSP 1 Score: 239.965 bits (611), Expect = 9.508e-63
Identity = 122/152 (80.26%), Postives = 137/152 (90.13%), Query Frame = 3
Query:  249 ASRRLGFSAA-DPLLTCHVASRLSSIKTKGTRAVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            ++RRLGFSA  D   + HVAS++ S++ KG R V++MAKKSVG+L+  DLKGKKVFVRADLNVPLDDNQNITDDTRIRAA+PTIK LI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSELLGI+VVKADDCIGPEVE LVASLPE GVLL
Sbjct:   38 SARRLGFSAVVDSRFSVHVASKVHSVRGKGARGVITMAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLL 189          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_TOBAC (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 4.719e-62
Identity = 126/153 (82.35%), Postives = 137/153 (89.54%), Query Frame = 3
Query:  255 RRLGFS--AADPLLTCHVASRLSSIKT--KGTRAVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            RRLGF+  AAD L T HVA++L S+K+  K  R V SMAKKSVG+L+ A+LKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHL+ NGAKVILSSHLGRPKGVTPK+SLAPLVPRLSELLGIQVVK +DCIGPEVEKLVASLPE GVLL
Sbjct:   39 RRLGFAGAAADSLFTNHVATKLRSLKSSSKPIRGVASMAKKSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLL 191          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_SPIOL (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea PE=2 SV=2)

HSP 1 Score: 225.713 bits (574), Expect = 1.856e-58
Identity = 115/138 (83.33%), Postives = 126/138 (91.30%), Query Frame = 3
Query:  297 HVASRLSSIKTKGT---RAVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            HV S+++S K++ T   R V SMAKKSVG+L+ ADLKGKKVFVRADLNVPLDD+QNITDDTRIRAA+PTIKHLI NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLG+QVVKADDCIGP+VEKLVA LPE GVLL
Sbjct:    7 HVLSKINSYKSQSTKPIRGVASMAKKSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLL 144          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_WHEAT (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.920e-55
Identity = 114/152 (75.00%), Postives = 129/152 (84.87%), Query Frame = 3
Query:  252 SRRLGFSA-ADPLLTCHVASRLSSIKT-KGTRAVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            +R L F+A ADP L  HVASR  +    +GTRAV +MAKKSVG+L+ ADL+GK+V VRADLNVPLDDNQNITDDTRIRAA+PTIK+L+ NGAKVIL+SHLGRPKGVTPKFSLAPLVPRLSELLGI+V KA+D IGPEVEKLVA L    VLL
Sbjct:   37 ARSLAFAAGADPRLAVHVASRCRAASAARGTRAVATMAKKSVGDLTAADLEGKRVLVRADLNVPLDDNQNITDDTRIRAAIPTIKYLLSNGAKVILTSHLGRPKGVTPKFSLAPLVPRLSELLGIEVKKAEDVIGPEVEKLVADLANGAVLL 188          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKY_WHEAT (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.168e-44
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 3
Query:  360 KKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            K+SVG L  ADLKGKKVFVRADLNVPLDD Q ITDDTRIRA++PTIK+L++ GAKVIL+SHLGRPKGVTPKFSL PLV RLSELLG++VV A DCIG EVEKL A+LP+ GVLL
Sbjct:    4 KRSVGTLGEADLKGKKVFVRADLNVPLDDAQKITDDTRIRASIPTIKYLLEKGAKVILASHLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEKLAAALPDGGVLL 117          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKY_TOBAC (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.525e-44
Identity = 90/114 (78.95%), Postives = 102/114 (89.47%), Query Frame = 3
Query:  360 KKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            KKSVG L  ADLKGK+VFVR DLNVPLD+N NITDDTRIRAAVPTIK+L+Q+G+ VIL+SHLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IGPEVEKLVA +PE GVLL
Sbjct:    4 KKSVGSLKEADLKGKRVFVRVDLNVPLDENFNITDDTRIRAAVPTIKYLMQHGSHVILASHLGRPKGVTPKYSLKPLVPRLSELLGVEVKIANDSIGPEVEKLVAEIPEGGVLL 117          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_VOLCA (Phosphoglycerate kinase, chloroplastic OS=Volvox carteri GN=PGK PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 4.023e-37
Identity = 94/161 (58.39%), Postives = 114/161 (70.81%), Query Frame = 3
Query:  234 RMPTSASRRLGFSA-ADPLLTCHVASRLS-SIKTKG-TRAVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDDNQ-NITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPK-GVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            R+   A R + FS+ A P+L    A  +S  I+  G +R VV   KKSVG+L  ADL+GK+VFVRADLNVPLD     ITDDTRIRAAVPT+K+L+ NGAKV+L+SHLGRPK G   K+ L P+V RLSELLG +V K DDCIGP VE+ VASL    +LL
Sbjct:   18 RVAAVAPRVVPFSSVARPVLRSTFAPEVSIDIRRAGRSRIVVEAVKKSVGDLGKADLEGKRVFVRADLNVPLDKKTLAITDDTRIRAAVPTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKEVKKVDDCIGPSVEQAVASLKSGELLL 178          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGKH_CHLRE (Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii PE=1 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.883e-35
Identity = 80/122 (65.57%), Postives = 94/122 (77.05%), Query Frame = 3
Query:  342 AVVSMAKKSVGELSGADLKGKKVFVRADLNVPLDD-NQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPK-GVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            +VV   KKSVG+L  ADL+GK+VFVRADLNVPLD     ITDDTRIRAAVPT+K+L+ NGAKV+L+SHLGRPK G   K+ L P+V RLSELLG  V K DDCIGPEVEK V ++    +LL
Sbjct:   56 SVVEAVKKSVGDLHKADLEGKRVFVRADLNVPLDKATLAITDDTRIRAAVPTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKPVTKVDDCIGPEVEKAVGAMKNGELLL 177          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGK_ANAVT (Phosphoglycerate kinase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=pgk PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 3.524e-33
Identity = 74/116 (63.79%), Postives = 84/116 (72.41%), Query Frame = 3
Query:  354 MAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            M+KK+V  LS AD+ GK+  VR D NVPLDD  NITDDTRIRAA+PTI+ L Q GAKVIL+SH GRPKGV  K  L P+  RLSELLG +VVK DD IG EV   VA+L    VLL
Sbjct:    1 MSKKTVASLSAADISGKRALVRVDFNVPLDDQGNITDDTRIRAALPTIQDLTQKGAKVILASHFGRPKGVDEKLRLTPVAKRLSELLGQEVVKTDDSIGDEVAAKVAALQNGQVLL 116          
BLAST of FC932475 vs. ExPASy Swiss-Prot
Match: PGK_ANASP (Phosphoglycerate kinase OS=Anabaena sp. (strain PCC 7120) GN=pgk PE=1 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 4.603e-33
Identity = 73/116 (62.93%), Postives = 84/116 (72.41%), Query Frame = 3
Query:  354 MAKKSVGELSGADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPESGVLL 701
            M+KK+V  LS AD+ GK+  VR D NVPLDD  NITDDTRIRAA+PTI+ L Q GAKVIL+SH GRPKGV  K  L P+  RLSELLG +V+K DD IG EV   VA+L    VLL
Sbjct:    1 MSKKTVASLSAADISGKRALVRVDFNVPLDDQGNITDDTRIRAALPTIQDLTQKGAKVILASHFGRPKGVDEKLRLTPVAKRLSELLGQEVIKTDDSIGDEVAAKVATLQNGQVLL 116          
The following BLAST results are available for this feature:
BLAST of FC932475 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PGKH_ARATH9.508e-6380.26Phosphoglycerate kinase, chloroplastic OS=Arabidop... [more]
PGKH_TOBAC4.719e-6282.35Phosphoglycerate kinase, chloroplastic OS=Nicotian... [more]
PGKH_SPIOL1.856e-5883.33Phosphoglycerate kinase, chloroplastic (Fragment) ... [more]
PGKH_WHEAT1.920e-5575.00Phosphoglycerate kinase, chloroplastic OS=Triticum... [more]
PGKY_WHEAT1.168e-4478.95Phosphoglycerate kinase, cytosolic OS=Triticum aes... [more]
PGKY_TOBAC1.525e-4478.95Phosphoglycerate kinase, cytosolic OS=Nicotiana ta... [more]
PGKH_VOLCA4.023e-3758.39Phosphoglycerate kinase, chloroplastic OS=Volvox c... [more]
PGKH_CHLRE2.883e-3565.57Phosphoglycerate kinase, chloroplastic OS=Chlamydo... [more]
PGK_ANAVT3.524e-3363.79Phosphoglycerate kinase OS=Anabaena variabilis (st... [more]
PGK_ANASP4.603e-3362.93Phosphoglycerate kinase OS=Anabaena sp. (strain PC... [more]

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Properties
Property NameValue
Genbank descriptionC34209A05EF PostHarveN Citrus clementina cDNA clone C34209A05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC932475 ID=FC932475; Name=FC932475; organism=Citrus clementina; type=EST; length=702bp
GGACGTCCACATCTTCTTCATCTTCCCCACACATATTCCTTCCCCATTCT
TCAATCAAACCAAAACCCACTTCCCATTTCCCCACTTTGTCGACAAAGTT
TCAATCTTTTGCGCCTTTACTACGCCCCTCCACTCCCGCCACATCAATGG
CTTCAGCCACCGCTCCCACTACTTTCTCTCTCGTCAAAACCGCCGCAAGT
TCATCCCTTTCTTCCCCACGCGCCTCCTTCCTGCGCATGCCCACTTCAGC
ATCCCGCAGGCTGGGGTTCTCCGCAGCCGACCCGCTGCTGACCTGCCATG
TGGCGTCGAGGCTGAGCTCGATTAAGACGAAGGGCACTCGTGCGGTGGTG
TCGATGGCGAAGAAGAGCGTGGGGGAGTTGAGTGGAGCTGATTTGAAAGG
GAAGAAGGTGTTTGTAAGAGCTGACTTGAATGTGCCTTTGGATGATAATC
AGAACATTACTGATGATACTAGAATCAGAGCTGCTGTTCCCACCATCAAG
CATTTGATTCAAAATGGAGCTAAAGTCATTCTTTCTAGCCACTTGGGAAG
ACCAAAGGGTGTTACTCCGAAATTCAGTTTGGCTCCTCTTGTGCCTCGTC
TTTCTGAACTCCTGGGCATTCAGGTTGTGAAGGCTGATGACTGCATTGGT
CCAGAGGTAGAAAAGCTTGTAGCCTCACTTCCTGAAAGCGGTGTTCTTCT
TC
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