FC932651

Overview
NameFC932651
Unique NameFC932651
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length703
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_ARATH (DNA topoisomerase 1 OS=Arabidopsis thaliana GN=TOP1 PE=1 SV=1)

HSP 1 Score: 372.474 bits (955), Expect = 1.238e-102
Identity = 188/234 (80.34%), Postives = 209/234 (89.32%), Query Frame = 2
Query:    2 TLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTGDGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            TLKVGNVECIPPNK+KFDFLGKDSIQYVNTVEVE  VYKAIGQFQ GK + DDLFD+LDT+KLNAHLKEL+PGLTAKVFRTYNASITLD ML++ T DGDV  KI VYQ+ANKEVAIICNHQRTVSKTH AQ+ +LT +IE+LK VLK+L+ +LDRAKKGKPPL + SDGK+ R+L P A E+K+A  +AKIEKMERDM TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI
Sbjct:  657 TLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPL-EGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 889          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_DAUCA (DNA topoisomerase 1 OS=Daucus carota GN=TOP1 PE=2 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 8.611e-88
Identity = 177/261 (67.82%), Postives = 200/261 (76.63%), Query Frame = 2
Query:    2 TLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGM-----LNKGTGDGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPE----------------------ALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            TLKV NVE   PN LKFDFLGKDSI+Y N VEVE  V+ AI QF++GK+  DDLFD+LDT+KLNAHLKELMPGLTAKVFRTYNASITLD M     L++ T  GDVA K+ VYQ ANKEVAIICNHQRTVSK+HSAQM RL EKIE+LKG+LK+L+ DL R  KGKPPL K+SDGK KRNL  +                      ++ +K+A TN KIEKMERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VPI
Sbjct:  507 TLKVENVETKRPNILKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPL-KNSDGKPKRNLNLKRYKGKLLKLIQKLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVPI 766          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_CAEEL (DNA topoisomerase 1 OS=Caenorhabditis elegans GN=top-1 PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.127e-50
Identity = 109/221 (49.32%), Postives = 138/221 (62.44%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTGDGD-VAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDFLGKDSI+Y N V VE  VYK +  F  GK  +DDLFD+LDTA LN HL+ LM GLT KVFRTYNASITL   L K T   D VA KI  Y RAN++VAI+CNHQR VSK     M +L +KI+D K  +K+    L                K  R    E  ++K      +++K++     K++ K +ALGTSK+NY+DPRITVAWCK+ EVP+
Sbjct:  574 VEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAAL----------------KSARGAEKEKAQKKYDRLKEQLKKLKISRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPL 778          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_CERAE (DNA topoisomerase 1 OS=Cercopithecus aethiops GN=TOP1 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.293e-48
Identity = 109/221 (49.32%), Postives = 141/221 (63.80%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTG-DGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDFLGKDSI+Y N V VE  V+K +  F   K+  DDLFD+L+T  LN HL++LM GLTAKVFRTYNASITL   L + T  D ++  KI  Y RAN+ VAI+CNHQR   KT    M  L  KI+  K  L D R DL ++ K    +MKD+  K+      +A++R       ++ K+E     +E+ K +ALGTSK+NYLDPRITVAWCK+  VPI
Sbjct:  527 VEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKEQLADARRDL-KSAKADAKVMKDAKTKKVVESKKKAVQR----LEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI 742          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_HUMAN (DNA topoisomerase 1 OS=Homo sapiens GN=TOP1 PE=1 SV=2)

HSP 1 Score: 191.815 bits (486), Expect = 2.994e-48
Identity = 109/221 (49.32%), Postives = 141/221 (63.80%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTG-DGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDFLGKDSI+Y N V VE  V+K +  F   K+  DDLFD+L+T  LN HL++LM GLTAKVFRTYNASITL   L + T  D ++  KI  Y RAN+ VAI+CNHQR   KT    M  L  KI+  K  L D R DL ++ K    +MKD+  K+      +A++R       ++ K+E     +E+ K +ALGTSK+NYLDPRITVAWCK+  VPI
Sbjct:  525 VEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDL-KSAKADAKVMKDAKTKKVVESKKKAVQR----LEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI 740          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_RAT (DNA topoisomerase 1 OS=Rattus norvegicus GN=Top1 PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 6.670e-48
Identity = 109/221 (49.32%), Postives = 140/221 (63.35%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTG-DGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDF GKDSI+Y N V VE  V+K +  F   K+  DDLFD+L+T  LN HL++LM GLTAKVFRTYNASITL   L + T  D +V  KI  Y RAN+ VAI+CNHQR   KT    M  L  KI+  K  L D R DL ++ K    +MKD+  K+      +A++R       ++ K+E     +E+ K +ALGTSK+NYLDPRITVAWCK+  VPI
Sbjct:  527 VEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARKDL-KSAKADAKVMKDAKTKKVVESKKKAVQR----LEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI 742          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_MOUSE (DNA topoisomerase 1 OS=Mus musculus GN=Top1 PE=1 SV=2)

HSP 1 Score: 190.274 bits (482), Expect = 8.712e-48
Identity = 109/221 (49.32%), Postives = 140/221 (63.35%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTG-DGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDF GKDSI+Y N V VE  V+K +  F   K+  DDLFD+L+T  LN HL++LM GLTAKVFRTYNASITL   L + T  D +V  KI  Y RAN+ VAI+CNHQR   KT    M  L  KI+  K  L D R DL ++ K    +MKD+  K+      +A++R       ++ K+E     +E+ K +ALGTSK+NYLDPRITVAWCK+  VPI
Sbjct:  527 VEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDL-KSAKADAKVMKDAKTKKVVESKKKAVQR----LEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI 742          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1_CRIGR (DNA topoisomerase 1 OS=Cricetulus griseus GN=TOP1 PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 8.712e-48
Identity = 109/221 (49.32%), Postives = 140/221 (63.35%), Query Frame = 2
Query:   44 LKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGTG-DGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            ++FDF GKDSI+Y N V VE  V+K +  F   K+  DDLFD+L+T  LN HL++LM GLTAKVFRTYNASITL   L + T  D +V  KI  Y RAN+ VAI+CNHQR   KT    M  L  KI+  K  L D R DL ++ K    +MKD+  K+      +A++R       ++ K+E     +E+ K +ALGTSK+NYLDPRITVAWCK+  VPI
Sbjct:  527 VEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDL-KSAKADAKVMKDAKTKKVVESKKKAVQR----LEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPI 742          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1M_HUMAN (DNA topoisomerase I, mitochondrial OS=Homo sapiens GN=TOP1MT PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 3.311e-47
Identity = 110/240 (45.83%), Postives = 141/240 (58.75%), Query Frame = 2
Query:    2 TLKVGNVECIPP-----NKLKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGT-GDGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            +L+V +V+  P      + ++FDFLGKD I+Y N V VE PVYK +  F   K   DDLFD+L T  LN HL+ELM GLTAKVFRTYNASITL   L   T  +  +A KI  Y RAN+ VAI+CNHQR    T    M  L  KI+  K  + + R +L RA+          DGK +  L  E   R +     ++ ++      KE+ K VALGTSK+NYLDPRI++AWCKR  VP+
Sbjct:  342 SLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEH---KAQGDGKSRSVL--EKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPV 576          
BLAST of FC932651 vs. ExPASy Swiss-Prot
Match: TOP1M_PANTR (DNA topoisomerase I, mitochondrial OS=Pan troglodytes GN=TOP1MT PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.643e-46
Identity = 109/240 (45.42%), Postives = 141/240 (58.75%), Query Frame = 2
Query:    2 TLKVGNVECIPP-----NKLKFDFLGKDSIQYVNTVEVELPVYKAIGQFQTGKKQNDDLFDKLDTAKLNAHLKELMPGLTAKVFRTYNASITLDGMLNKGT-GDGDVAVKIDVYQRANKEVAIICNHQRTVSKTHSAQMSRLTEKIEDLKGVLKDLRVDLDRAKKGKPPLMKDSDGKRKRNLAPEALERKMAXTNAKIEKMERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPI 703
            +L+V +V+  P      + ++FDFLGKD I+Y N V VE PVYK +  F   K   D+LFD+L T  LN HL+ELM GLTAKVFRTYNASITL   L   T  +  +A KI  Y RAN+ VAI+CNHQR    T    M  L  KI+  K  + + R +L RA+          DGK +  L  E   R +     ++ ++      KE+ K VALGTSK+NYLDPRI++AWCKR  VP+
Sbjct:  342 SLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMESKGPRDNLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEH---KAQGDGKSRSVL--EKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPV 576          
The following BLAST results are available for this feature:
BLAST of FC932651 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
TOP1_ARATH1.238e-10280.34DNA topoisomerase 1 OS=Arabidopsis thaliana GN=TOP... [more]
TOP1_DAUCA8.611e-8867.82DNA topoisomerase 1 OS=Daucus carota GN=TOP1 PE=2 ... [more]
TOP1_CAEEL7.127e-5049.32DNA topoisomerase 1 OS=Caenorhabditis elegans GN=t... [more]
TOP1_CERAE2.293e-4849.32DNA topoisomerase 1 OS=Cercopithecus aethiops GN=T... [more]
TOP1_HUMAN2.994e-4849.32DNA topoisomerase 1 OS=Homo sapiens GN=TOP1 PE=1 S... [more]
TOP1_RAT6.670e-4849.32DNA topoisomerase 1 OS=Rattus norvegicus GN=Top1 P... [more]
TOP1_MOUSE8.712e-4849.32DNA topoisomerase 1 OS=Mus musculus GN=Top1 PE=1 S... [more]
TOP1_CRIGR8.712e-4849.32DNA topoisomerase 1 OS=Cricetulus griseus GN=TOP1 ... [more]
TOP1M_HUMAN3.311e-4745.83DNA topoisomerase I, mitochondrial OS=Homo sapiens... [more]
TOP1M_PANTR1.643e-4645.42DNA topoisomerase I, mitochondrial OS=Pan troglody... [more]

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Properties
Property NameValue
Genbank descriptionC34210H07EF PostHarveN Citrus clementina cDNA clone C34210H07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC932651 ID=FC932651; Name=FC932651; organism=Citrus clementina; type=EST; length=703bp
TACTTTAAAAGTAGGGAATGTTGAGTGTATCCCACCAAATAAATTGAAGT
TTGACTTCCTTGGTAAAGATTCGATCCAATATGTAAACACAGTTGAAGTT
GAGCTTCCTGTCTACAAGGCAATTGGACAATTTCAAACTGGAAAGAAGCA
AAATGATGATCTTTTCGACAAACTGGATACAGCTAAACTGAATGCTCACC
TGAAGGAACTCATGCCTGGACTTACAGCCAAAGTCTTCCGTACATATAAT
GCCTCTATCACATTGGATGGGATGTTGAATAAGGGAACAGGAGATGGTGA
TGTTGCAGTAAAAATAGATGTTTATCAGCGTGCAAACAAAGAGGTTGCTA
TTATTTGTAATCATCAGCGAACTGTTTCAAAAACTCATTCTGCACAGATG
TCAAGATTAACTGAGAAGATAGAAGACCTTAAGGGTGTTCTGAAAGACTT
GAGAGTTGATTTAGACAGAGCTAAAAAAGGGAAGCCCCCATTGATGAAGG
ATTCTGATGGAAAGAGAAAGAGGAACTTGGCACCCGAAGCATTGGAGAGG
AAGATGGCTCANACCAATGCAAAGATTGAGAAAATGGAACGAGACATGAA
GACCAAAGAGGATCTGAAAACCGTGGCATTAGGCACGTCCAAGATCAATT
ACCTTGACCCTAGGATCACAGTTGCTTGGTGCAAGCGACACGAAGTTCCC
ATT
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