CF417784

Overview
NameCF417784
Unique NameCF417784
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK_DICDI (S-adenosylmethionine synthetase OS=Dictyostelium discoideum GN=metK PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 8.134e-70
Identity = 67/94 (71.28%), Postives = 79/94 (84.04%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTVEY  +   + PVR H ++ISTQHDE VTN++I ADLKE VIK V+P +YLD+ TI+HLNPSGRFVIGGP GD+GLTGRKIIID+YGGWGA
Sbjct:  170 QVTVEYKKEGHKITPVRVHTIVISTQHDEKVTNEQIRADLKELVIKAVVPAQYLDDNTIYHLNPSGRFVIGGPMGDSGLTGRKIIIDSYGGWGA 263

HSP 2 Score: 133.65 bits (335), Expect = 8.134e-70
Identity = 71/112 (63.39%), Postives = 82/112 (73.21%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFWQG*P 619
            HGGGAFSGKD TKVDRSGAY  R  AKS+VA GLA RC+VQVSY+IGV +PLSVFVD+YGTGK  D EIL I+  +FD RPG +  +L L R     F KTA+ GHF +  P
Sbjct:  264 HGGGAFSGKDATKVDRSGAYAARWIAKSLVAAGLADRCLVQVSYSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNNFDLRPGCLMRDLQLTR---PIFQKTASGGHFGRNDP 372

HSP 3 Score: 27.335 bits (59), Expect = 8.134e-70
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  603 FGRDDPDFTW 632
            FGR+DPDFTW
Sbjct:  367 FGRNDPDFTW 376
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 9.474e-65
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  D GA++P+R H ++IS QHDE V  DE+   LKE VIK V+P KYLDE TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA 276

HSP 2 Score: 124.79 bits (312), Expect = 9.474e-65
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 142.124 bits (357), Expect = 9.474e-65
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  D GA++P+R H ++IS QHDE V  DE+   LKE VIK V+P KYLDE TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA 276

HSP 2 Score: 124.79 bits (312), Expect = 9.474e-65
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 9.474e-65
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  D GA++P+R H ++IS QHDE V  DE+   LKE VIK V+P KYLDE TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA 276

HSP 2 Score: 124.79 bits (312), Expect = 9.474e-65
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 9.474e-65
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  D GA++P+R H ++IS QHDE V  DE+   LKE VIK V+P KYLDE TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA 276

HSP 2 Score: 124.79 bits (312), Expect = 9.474e-65
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK2_RAT (S-adenosylmethionine synthetase isoform type-2 OS=Rattus norvegicus GN=Mat2a PE=2 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.611e-64
Identity = 65/94 (69.15%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  D GA++P+R H ++IS QHDE V  DE+   LKE +IK V+P KYLDE TI+HL PSGRFVIGGP GDAGLTGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDRGAVIPIRVHTIVISVQHDEEVCLDEMRDALKEKLIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA 276

HSP 2 Score: 124.405 bits (311), Expect = 1.611e-64
Identity = 63/107 (58.88%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK +FD RPG++  +LDLK+     + +TAAYGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKNNFDLRPGVIVRDLDLKK---PIYQRTAAYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK1_HUMAN (S-adenosylmethionine synthetase isoform type-1 OS=Homo sapiens GN=MAT1A PE=1 SV=2)

HSP 1 Score: 141.739 bits (356), Expect = 4.657e-64
Identity = 63/94 (67.02%), Postives = 78/94 (82.98%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  DNGA++PVR H ++IS QH+E +T +E+   LKE VI+ V+P KYLDE T++HL PSGRFVIGGP GDAG+TGRKII+DTYGGWGA
Sbjct:  183 QVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGA 276

HSP 2 Score: 122.865 bits (307), Expect = 4.657e-64
Identity = 62/107 (57.94%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV EPLS+ + +YGT +  ++E+L +V ++FD RPG++  +LDLK+     + KTA YGHF
Sbjct:  277 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKK---PIYQKTACYGHF 380
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK1_MOUSE (S-adenosylmethionine synthetase isoform type-1 OS=Mus musculus GN=Mat1a PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 6.072e-64
Identity = 63/94 (67.02%), Postives = 77/94 (81.91%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  DNGA++PVR H ++IS QH+E +T + +   LKE VIK V+P KYLDE T++HL PSGRFVIGGP GDAG+TGRKII+DTYGGWGA
Sbjct:  184 QVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEAMQEALKEQVIKAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGA 277

HSP 2 Score: 123.635 bits (309), Expect = 6.072e-64
Identity = 62/107 (57.94%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV EPLS+ + +YGT    ++E+L++V ++FD RPG++  +LDLK+     + KTA YGHF
Sbjct:  278 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSNKTERELLEVVNKNFDLRPGVIVRDLDLKK---PIYQKTACYGHF 381
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK1_RAT (S-adenosylmethionine synthetase isoform type-1 OS=Rattus norvegicus GN=Mat1a PE=1 SV=2)

HSP 1 Score: 141.354 bits (355), Expect = 2.289e-63
Identity = 64/94 (68.09%), Postives = 77/94 (81.91%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  DNGA++PVR H ++IS QH+E +T + +   LKE VIK V+P KYLDE TI+HL PSGRFVIGGP GDAG+TGRKII+DTYGGWGA
Sbjct:  184 QVTVQYVQDNGAVIPVRVHTIVISVQHNEDITLEAMREALKEQVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGA 277

HSP 2 Score: 120.939 bits (302), Expect = 2.289e-63
Identity = 63/108 (58.33%), Postives = 80/108 (74.07%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDK-EILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV EPLS+ + +YGT K  ++ E+L++V ++FD RPG++  +LDLK+     + KTA YGHF
Sbjct:  278 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSKKTERDELLEVVNKNFDLRPGVIVRDLDLKK---PIYQKTACYGHF 382
[Close]
BLAST of CF417784 vs. ExPASy Swiss-Prot
Match: METK1_BOVIN (S-adenosylmethionine synthetase isoform type-1 OS=Bos taurus GN=MAT1A PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.289e-63
Identity = 62/94 (65.96%), Postives = 78/94 (82.98%), Query Frame = 1 
Query:    1 QVTVEYYNDNGAMVPVRGHXVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGA 282
            QVTV+Y  DNGA++P+R H V+IS QH+E +T +++   LKE VI+ V+P +YLDE TI+HL PSGRFVIGGP GDAG+TGRKII+DTYGGWGA
Sbjct:  184 QVTVQYTQDNGAVIPMRVHTVVISVQHNEDITLEDMRRALKEQVIRAVVPARYLDEDTIYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGA 277

HSP 2 Score: 123.25 bits (308), Expect = 2.289e-63
Identity = 62/107 (57.94%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  284 HGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 604
            HGGGAFSGKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV EPLS+ + +YGT +  ++E+L +V ++FD RPG++  +LDLK+     + KTA YGHF
Sbjct:  278 HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVNKNFDLRPGVIVRDLDLKK---PIYQKTACYGHF 381
The following BLAST results are available for this feature:
BLAST of CF417784 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_DICDI8.134e-7071.28S-adenosylmethionine synthetase OS=Dictyostelium d... [more]
METK2_PONAB9.474e-6570.21S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MOUSE9.474e-6570.21S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MACFA9.474e-6570.21S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_HUMAN9.474e-6570.21S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_RAT1.611e-6469.15S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK1_HUMAN4.657e-6467.02S-adenosylmethionine synthetase isoform type-1 OS=... [more]
METK1_MOUSE6.072e-6467.02S-adenosylmethionine synthetase isoform type-1 OS=... [more]
METK1_RAT2.289e-6368.09S-adenosylmethionine synthetase isoform type-1 OS=... [more]
METK1_BOVIN2.289e-6365.96S-adenosylmethionine synthetase isoform type-1 OS=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-714 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-010_B05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417784 ID=CF417784; Name=CF417784; organism=Citrus sinensis; type=EST; length=651bp
CAGGTCACTGTTGAGTACTACAATGACAATGGTGCCATGGTCCCAGTCCG
NGGCCACANGGTCCTCATTTCAACCCAGCATGATGAGACTGTCACAAATG
ATGAAATTGCTGCTGACCTTAAGGAGCACGTCATTAAGCCTGTCATCCCC
GAGAAGTACCTTGATGAGAAGACCATCTTCCACCTTAATCCATCAGGTCG
TTTTGTCATTGGTGGGCCTCATGGTGATGCTGGTCTCACTGGACGTAAGA
TCATCATCGACACTTATGGTGGCTGGGGAGCCCCATGGTGGCGGTGCTTT
CTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATACATTGTTA
GGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCTTGCTCGCAGGTGCATT
GTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCTGTTTTTGT
GGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCTCAAAATTG
TGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCAATTTGGAT
CTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCCTATGGACA
CTTTTGGCAGGGATGACCCAGACTTCACTTGGGGAAGTTGTGAAGCCCCT
C
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