CF504605

Overview
NameCF504605
Unique NameCF504605
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length431
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_ARATH (Phosphoenolpyruvate carboxykinase [ATP] OS=Arabidopsis thaliana GN=PCKA PE=1 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 3.517e-71
Identity = 97/102 (95.10%), Postives = 100/102 (98.04%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            +DLSREKEPDIWNAIKFG VLENVVFDEHTREVDYSDKSVTENTRAAYPIE+IPNAKIPCVGPHP NVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL+
Sbjct:  411 VDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAYPIEFIPNAKIPCVGPHPTNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALV 512

HSP 2 Score: 83.1889 bits (204), Expect = 3.517e-71
Identity = 39/42 (92.86%), Postives = 40/42 (95.24%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKM+  G
Sbjct:  513 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMKSQG 554
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA2_UROPA (Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Urochloa panicoides GN=PCK2 PE=2 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 1.448e-69
Identity = 92/102 (90.20%), Postives = 100/102 (98.04%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            IDLS+EKEPDIWNAIKFG VLENVVF+E TREVDY+DKS+TENTRAAYPIE+IPNAKIPCVGPHPKNVILLACDA+GVLPPVSKLNLAQTMYHFISGYTA++
Sbjct:  366 IDLSQEKEPDIWNAIKFGTVLENVVFNERTREVDYADKSITENTRAAYPIEFIPNAKIPCVGPHPKNVILLACDAYGVLPPVSKLNLAQTMYHFISGYTAIV 467

HSP 2 Score: 85.1149 bits (209), Expect = 1.448e-69
Identity = 39/42 (92.86%), Postives = 41/42 (97.62%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTEDG+KEPTATFSACFGAAFIM HPTKYAAMLAEKMQK+G
Sbjct:  468 AGTEDGVKEPTATFSACFGAAFIMYHPTKYAAMLAEKMQKYG 509
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_MAIZE (Phosphoenolpyruvate carboxykinase [ATP] OS=Zea mays PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 5.458e-69
Identity = 94/102 (92.16%), Postives = 98/102 (96.08%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            IDL++EKEPDIWNAIKFG VLENVVFDEHTREVDY+D SVTENTRAAYPIEYIP AKIPCVGPHPKNVILLACDAFGVLPPVSKL LAQTMYHFISGYTAL+
Sbjct:  406 IDLAQEKEPDIWNAIKFGTVLENVVFDEHTREVDYADYSVTENTRAAYPIEYIPIAKIPCVGPHPKNVILLACDAFGVLPPVSKLILAQTMYHFISGYTALV 507

HSP 2 Score: 85.1149 bits (209), Expect = 5.458e-69
Identity = 40/42 (95.24%), Postives = 41/42 (97.62%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTEDGIKEP ATFSACFGAAFIMLHPTKYAAMLAEKMQK+G
Sbjct:  508 AGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMQKYG 549
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA1_UROPA (Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Urochloa panicoides GN=PCK1 PE=1 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 5.461e-69
Identity = 91/102 (89.22%), Postives = 99/102 (97.06%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            IDLS+EKEPDIWNAI FG VLENVVF+E TREVDY+DKS+TENTRAAYPIE+IPNAKIPCVGPHPKNVILLACDA+GVLPPVSKLNLAQTMYHFISGYTA++
Sbjct:  364 IDLSKEKEPDIWNAITFGTVLENVVFNERTREVDYADKSITENTRAAYPIEFIPNAKIPCVGPHPKNVILLACDAYGVLPPVSKLNLAQTMYHFISGYTAIV 465

HSP 2 Score: 85.1149 bits (209), Expect = 5.461e-69
Identity = 39/42 (92.86%), Postives = 41/42 (97.62%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            AGTEDG+KEPTATFSACFGAAFIM HPTKYAAMLAEKMQK+G
Sbjct:  466 AGTEDGVKEPTATFSACFGAAFIMYHPTKYAAMLAEKMQKYG 507
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CUCSA (Phosphoenolpyruvate carboxykinase [ATP] OS=Cucumis sativus GN=PCK PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 1.892e-52
Identity = 97/102 (95.10%), Postives = 100/102 (98.04%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALL 308
            IDLSREKEPDIWNAIKFG VLENVVFDEHTREVDYS+KSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKL+L QTMYHFISGYTAL+
Sbjct:  410 IDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSEKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLSLPQTMYHFISGYTALV 511

HSP 2 Score: 82.8037 bits (203), Expect = 6.358e-16
Identity = 43/76 (56.58%), Postives = 52/76 (68.42%), Query Frame = 2 
Query:  203 CHPFGM*CFWCTPTSEQAEPGTNNVPFHQWLHCFAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHG 430
            C  FG+      P S+ + P T       +    AGTE+G+KEP ATFSACFGAAFIMLHP++YAAMLAEKM+KHG
Sbjct:  482 CDAFGV----LPPVSKLSLPQTMYHFISGYTALVAGTEEGVKEPQATFSACFGAAFIMLHPSRYAAMLAEKMKKHG 553
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_NEUCR (Phosphoenolpyruvate carboxykinase [ATP] OS=Neurospora crassa GN=pck-1 PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.365e-51
Identity = 76/101 (75.25%), Postives = 89/101 (88.12%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGP-HPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I LS EKEPDI+NAI++G+VLENVVF+  TREVDY D ++TENTR AYPIEYIPNAKIPC+ P HPKN+ILL CDA GVLPP+SKL+ AQTM+HFISGYT+
Sbjct:  305 IGLSAEKEPDIFNAIRYGSVLENVVFNPETREVDYGDATLTENTRCAYPIEYIPNAKIPCLSPNHPKNIILLTCDARGVLPPISKLDSAQTMFHFISGYTS 405

HSP 2 Score: 62.003 bits (149), Expect = 1.365e-51
Identity = 27/41 (65.85%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTEDGI EP ATFS+CF   F+ LHP +YA MLAEK++ H
Sbjct:  408 AGTEDGILEPQATFSSCFAQPFLALHPMRYAKMLAEKIENH 448
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_DICDI (Phosphoenolpyruvate carboxykinase [ATP] OS=Dictyostelium discoideum GN=pckA PE=1 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 1.780e-51
Identity = 71/100 (71.00%), Postives = 89/100 (89.00%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            IDLSREKEP+I++AIKFGAVLENVV++E++R+VDY+D S+TENTR AYP+E+IPNAK P +  HPKN+I+L CDAFG+LPPVS+L+  Q MYHFI GYTA
Sbjct:  307 IDLSREKEPEIFDAIKFGAVLENVVYNEYSRKVDYNDVSITENTRCAYPLEHIPNAKFPAIAGHPKNIIMLTCDAFGILPPVSRLDANQVMYHFIQGYTA 406

HSP 2 Score: 62.7734 bits (151), Expect = 1.780e-51
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EPTATFS+C+G  FI+ HPTKYA MLA ++ KH
Sbjct:  409 AGTEVGVTEPTATFSSCYGEPFIVWHPTKYAEMLASQLHKH 449
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_KLULA (Phosphoenolpyruvate carboxykinase [ATP] OS=Kluyveromyces lactis GN=PCK1 PE=3 SV=2)

HSP 1 Score: 154.451 bits (389), Expect = 2.521e-50
Identity = 71/101 (70.30%), Postives = 86/101 (85.15%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGP-HPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            + LS EKEP+I+NAIKFG+VLEN+++D +TREVDY D ++TENTR AYPIEYIP+AKIPC+   HPKN++LL CDA GVLPPVSKL   Q MYHFISGYT+
Sbjct:  286 LGLSAEKEPEIFNAIKFGSVLENIIYDPNTREVDYEDSTITENTRCAYPIEYIPSAKIPCLADHHPKNIVLLTCDASGVLPPVSKLTPDQVMYHFISGYTS 386

HSP 2 Score: 64.3142 bits (155), Expect = 2.521e-50
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP ATFS+CFG  F+ LHP KYA MLAEKM +H
Sbjct:  389 AGTEQGVTEPEATFSSCFGQPFLSLHPMKYATMLAEKMAEH 429
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_CANGA (Phosphoenolpyruvate carboxykinase [ATP] OS=Candida glabrata GN=PCK1 PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 1.611e-49
Identity = 71/101 (70.30%), Postives = 87/101 (86.14%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGP-HPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I+LSREKEP+I++AI+FG+VLENV++D  + EVDY D S+TENTR AYPI++IP+AKIPC+ P HPKN+ILL CDA GVLPPVSKL   Q MYHFISGYT+
Sbjct:  288 INLSREKEPEIFDAIRFGSVLENVIYDSESHEVDYDDSSITENTRCAYPIDFIPSAKIPCLAPAHPKNIILLTCDASGVLPPVSKLTPEQVMYHFISGYTS 388

HSP 2 Score: 61.6178 bits (148), Expect = 1.611e-49
Identity = 26/41 (63.41%), Postives = 31/41 (75.61%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP  TFS+CFG  F+ LHP +YA MLAEKM +H
Sbjct:  391 AGTEQGVTEPEPTFSSCFGQPFLSLHPMRYATMLAEKMHEH 431
[Close]
BLAST of CF504605 vs. ExPASy Swiss-Prot
Match: PCKA_ASHGO (Phosphoenolpyruvate carboxykinase [ATP] OS=Ashbya gossypii GN=PCK1 PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 2.972e-48
Identity = 70/101 (69.31%), Postives = 83/101 (82.18%), Query Frame = 3 
Query:    3 IDLSREKEPDIWNAIKFGAVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGP-HPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTA 302
            I LS EKEP+I+NAIK+G+VLENVV+D  TR VDY D S+TENTR AYPIEYIP+A+IPC+   HP N++LL CDA GVLPPVS+L   Q MYHFISGYT+
Sbjct:  285 IGLSGEKEPEIFNAIKYGSVLENVVYDPVTRVVDYEDSSITENTRCAYPIEYIPSAQIPCLSENHPSNIVLLTCDASGVLPPVSRLTPEQVMYHFISGYTS 385

HSP 2 Score: 64.6994 bits (156), Expect = 2.972e-48
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 2 
Query:  305 AGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKH 427
            AGTE G+ EP ATFS+CFG  F+ LHP KYA MLAEKM +H
Sbjct:  388 AGTEQGVTEPEATFSSCFGQPFLALHPMKYATMLAEKMSQH 428
The following BLAST results are available for this feature:
BLAST of CF504605 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_ARATH3.517e-7195.10Phosphoenolpyruvate carboxykinase [ATP] OS=Arabido... [more]
PCKA2_UROPA1.448e-6990.20Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Uroch... [more]
PCKA_MAIZE5.458e-6992.16Phosphoenolpyruvate carboxykinase [ATP] OS=Zea may... [more]
PCKA1_UROPA5.461e-6989.22Phosphoenolpyruvate carboxykinase [ATP] 1 OS=Uroch... [more]
PCKA_CUCSA1.892e-5295.10Phosphoenolpyruvate carboxykinase [ATP] OS=Cucumis... [more]
PCKA_NEUCR1.365e-5175.25Phosphoenolpyruvate carboxykinase [ATP] OS=Neurosp... [more]
PCKA_DICDI1.780e-5171.00Phosphoenolpyruvate carboxykinase [ATP] OS=Dictyos... [more]
PCKA_KLULA2.521e-5070.30Phosphoenolpyruvate carboxykinase [ATP] OS=Kluyver... [more]
PCKA_CANGA1.611e-4970.30Phosphoenolpyruvate carboxykinase [ATP] OS=Candida... [more]
PCKA_ASHGO2.972e-4869.31Phosphoenolpyruvate carboxykinase [ATP] OS=Ashbya ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-094 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_C03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504605 ID=CF504605; Name=CF504605; organism=Citrus sinensis; type=EST; length=431bp
GCATTGATCTCTCAAGGGAGAAGGAGCCTGATATCTGGAATGCCATCAAG
TTTGGCGCTGTGTTGGAAAATGTCGTGTTTGATGAGCATACCAGAGAGGT
GGATTATTCTGACAAATCTGTCACAGAGAACACTCGTGCTGCCTACCCCA
TTGAGTATATCCCCAATGCAAAGATACCATGCGTTGGTCCTCATCCCAAG
AATGTCATCCTTTTGGCATGTGATGCTTTTGGTGTACTCCCACCAGTGAG
CAAGCTGAACCTGGCACAAACAATGTACCATTTCATCAGTGGCTACACTG
CTTTGCTGGAACAGAGGATGGTATTAAGGAGCCAACGGCAACATTTTCAG
CTTGCTTTGGTGCAGCATTTATCATGCTGCATCCTACCAAGTATGCAGCA
ATGCTGGCTGAGAAGATGCAGAAGCATGGTG
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