CF509765

Overview
NameCF509765
Unique NameCF509765
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length564
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: 7OMT9_MEDSA (Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.153e-51
Identity = 94/187 (50.27%), Postives = 131/187 (70.05%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G  FWD+L++N      FN AM SDS++  L ++DC  +F GL S+VDVGGGTG  A+II E FP +KC V D P  V ++  ++NL YV GDMF  IP ADA   K + H + D+DCL++LKKC+EA+ ++G+RGKV I+D+VINEK+D+ ++T+ KLL D  M   + G+ER E+EW+ LF+ AG
Sbjct:  147 GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINEKKDENQVTQIKLLMDVNMAC-LNGKERNEEEWKKLFIEAG 332          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: 7OMT6_MEDSA (Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 1.578e-50
Identity = 93/187 (49.73%), Postives = 131/187 (70.05%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G  FWD+L++N      FN AM SDS++  L ++DC  +F GL S+VDVGGGTG  A+II E FP +KC V D P  V ++  ++NL YV GDMF  IP ADA   K + H + D+DCL++LKKC+EA+ ++G+RGKV I+D+VI+EK+D+ ++T+ KLL D  M   + G+ER E+EW+ LF+ AG
Sbjct:  147 GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMAC-LNGKERNEEEWKKLFIEAG 332          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: 7OMT8_MEDSA (Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 4.592e-50
Identity = 92/187 (49.20%), Postives = 131/187 (70.05%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G  FWD+L++N      FN AM SDS++  L ++DC  +F GL S+VDVGGGTG  A+II E FP +KC V D P  V ++  ++NL YV GDMF  IP ADA   K + H + D+DCL++LKKC+EA+ ++G+RGKV I+D+VI++K+D+ ++T+ KLL D  M   + G+ER E+EW+ LF+ AG
Sbjct:  147 GSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDKKKDENQVTQIKLLMDVNMAC-LNGKERNEEEWKKLFIEAG 332          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.918e-44
Identity = 87/188 (46.28%), Postives = 123/188 (65.43%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMA-TLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G   W Y+ ++    + FN+AM +DS +  + +VK+C  IF G+ +LVDVGGGTG   R I+ AFP IKCTV DLPH + D P    +  V GDMF+FIP ADA   K + H +DD++C+++LK+C+EA+   G  GKV+IVDIV+N  + +   T+ +L  D  M  N GG+ERTE+EW+ L  +AG
Sbjct:  142 GTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKG--GKVIIVDIVLN-VQSEHPYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAG 326          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: EOMT1_OCIBA (Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 6.011e-42
Identity = 86/188 (45.74%), Postives = 118/188 (62.77%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEM-ATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G  FW+ L    +  + F++AM  DS + A +  KD K + +G+R+LVDVGGG G  A+ I EA P IKCTV+DLPH V  +  TDNL Y+ GDMFQ IP+ADA   K++ H +DD + LK+LKKC++A+      GKV+I+D+V+    D  E+ E +L FD  M      +ERT  EWE L  +AG
Sbjct:  153 GCTFWEKLANEPSKGRFFDEAMSCDSRLIAHVFTKDYKHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHVVAGLESTDNLNYIGGDMFQSIPSADAILLKSIIHDWDDVEGLKILKKCKDAVVMG---GKVIIIDVVVGVNHDIDEVLEDQLHFDMAMMCYFNAKERTMSEWEKLIYDAG 337          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.284e-41
Identity = 86/188 (45.74%), Postives = 118/188 (62.77%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEM-ATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G  FW+ L    ++ + F++AM  DS + A ++ KD K +  G+R+LVDVGGG G  A+ I EA P +KCTVLDLPH V  +  TD L Y+ GDMFQ IP+ADA   K + H +DDE+ LK+LK+C++A+   G  GKV+I+D+V+    D  E+ E +L FD  M      +ERT  EWE L   AG
Sbjct:  152 GCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTKDYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTDKLSYIGGDMFQSIPSADAILLKFIIHDWDDEEGLKILKRCKDAV---GIGGKVIIIDVVVGVNHDVDEVLEDQLHFDMAMMSYFNAKERTMNEWEKLISAAG 336          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.253e-39
Identity = 77/188 (40.96%), Postives = 122/188 (64.89%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATL-IVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G+  W+YL  +   ++ FN+ M  ++ + T  ++   + +FQG+ SLVDVGGG G   + IS+AFP IKCT+ DLPH + +     N++ + GDMF+ +P+A A   K + H ++DED +K+LK+CR A+  +G  GKV+IVD+ ++E E D EL+  +L+ D  M  N GG+ERT++ WE +  +AG
Sbjct:  146 GMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDG--GKVIIVDVALDE-ESDHELSSTRLILDIDMLVNTGGKERTKEVWEKIVKSAG 330          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: ZRP4_MAIZE (O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.532e-37
Identity = 76/188 (40.43%), Postives = 113/188 (60.11%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMAT-LIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAG 563
            G   W+    +       N  + SDS++   + +K    +FQG+ SLVDVGGG GA A+ IS+AFP +KC+VLDL H V   P   +++++ GDMF+ IP ADA   K+V H +D +DC+K+LK C++AI      GKV+I+++V+     D +  E + +FD  + F + G ER EQEW  +F  AG
Sbjct:  158 GRGIWELTKDDATFDALVNDGLASDSQLIVDVAIKQSAEVFQGISSLVDVGGGIGAAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVGAGPSDMKHKEMQAIFDVYIMF-INGMERDEQEWSKIFSEAG 344          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: OMT1_CHRAE (Quercetin 3-O-methyltransferase 1 OS=Chrysosplenium americanum GN=OMT1 PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.641e-31
Identity = 74/188 (39.36%), Postives = 107/188 (56.91%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTL-MNFNVGGRERTEQEWESLFVNAG 563
            G+  ++Y   +    K FN+ M   S +    V      FQGL SLVDVGGGTGA   +I   +P I+C   DLPH + D P+   +++V GDMF  +P  DA F K + H + DE CLKLLK C +A+ +N   GKV++ + ++ E  D    T+  +  D + +  N GG+ERTE+E+E+L   AG
Sbjct:  135 GMSSFEYHGTDPRFNKVFNRGMSDHSTITMKKVFQAYQGFQGLTSLVDVGGGTGATLTMILSKYPTIRCINFDLPHVIEDAPEYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKLLKNCYDALPNN---GKVILAECILPEVPDSSLATKGVVHIDVITVAHNPGGKERTEKEFEALAKAAG 319          
BLAST of CF509765 vs. ExPASy Swiss-Prot
Match: OMT2_CHRAE (Quercetin 3-O-methyltransferase 2 OS=Chrysosplenium americanum GN=OMT2 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.143e-31
Identity = 74/188 (39.36%), Postives = 107/188 (56.91%), Query Frame = 3
Query:    3 GLKFWDYLNQNTALTKRFNQAMESDSEMATLIVKDCKPIFQGLRSLVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVVGDMFQFIPAADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDRELTEAKLLFDTL-MNFNVGGRERTEQEWESLFVNAG 563
            G+  ++Y   +    K FN+ M   S +    V      FQGL SLVDVGGGTGA   +I   +P I+C   DLPH + D P+   +++V GDMF  +P  DA F K + H + DE CLKLLK C +A+ +N   GKV++ + ++ E  D    T+  +  D + +  N GG+ERTE+E+E+L   AG
Sbjct:  135 GMSSFEYHGTDPRFNKVFNRGMSDHSTITMKKVFQTYQGFQGLTSLVDVGGGTGATLTMILSKYPTIRCINFDLPHVIEDAPEYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKLLKNCYDALPNN---GKVILAECILPEVPDSSLATKGVVHIDVITVAHNPGGKERTEKEFEALAKAAG 319          
The following BLAST results are available for this feature:
BLAST of CF509765 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
7OMT9_MEDSA4.153e-5150.27Isoflavone-7-O-methyltransferase 9 OS=Medicago sat... [more]
7OMT6_MEDSA1.578e-5049.73Isoflavone-7-O-methyltransferase 6 OS=Medicago sat... [more]
7OMT8_MEDSA4.592e-5049.20Isoflavone-7-O-methyltransferase 8 OS=Medicago sat... [more]
6OMT_COPJA4.918e-4446.28(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis... [more]
EOMT1_OCIBA6.011e-4245.74Eugenol O-methyltransferase OS=Ocimum basilicum GN... [more]
CVMT1_OCIBA2.284e-4145.74Chavicol O-methyltransferase OS=Ocimum basilicum G... [more]
4OMT_COPJA1.253e-3940.963'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltran... [more]
ZRP4_MAIZE1.532e-3740.43O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 ... [more]
OMT1_CHRAE1.641e-3139.36Quercetin 3-O-methyltransferase 1 OS=Chrysospleniu... [more]
OMT2_CHRAE2.143e-3139.36Quercetin 3-O-methyltransferase 2 OS=Chrysospleniu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-273 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-81_A11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF509765 ID=CF509765; Name=CF509765; organism=Citrus sinensis; type=EST; length=564bp
GCGGGTTGAAGTTTTGGGACTATCTGAACCAGAACACTGCATTAACCAAG
CGGTTCAACCAAGCAATGGAAAGCGATTCTGAAATGGCAACCTTGATTGT
CAAAGACTGCAAGCCAATCTTTCAAGGGTTGCGCTCCCTGGTTGATGTAG
GAGGTGGAACGGGTGCATTCGCGAGAATCATATCTGAAGCATTCCCCGGC
ATCAAGTGCACAGTACTTGACCTCCCACATGCGGTCACTGACATGCCTCA
GACTGACAACTTGAAGTACGTAGTGGGTGATATGTTTCAGTTTATTCCTG
CGGCAGACGCCTTTTTCTTCAAGACGGTTTTTCATTTATTTGATGACGAG
GATTGCTTGAAGCTATTGAAGAAATGCAGAGAAGCTATCGCAAGCAATGG
CGAGAGAGGAAAGGTATTAATCGTCGATATTGTAATCAATGAAAAGGAAG
ATGACCGTGAATTAACAGAAGCAAAGCTCTTGTTTGACACTTTGATGAAC
TTTAATGTTGGTGGAAGGGAGAGAACTGAGCAAGAATGGGAAAGTCTTTT
CGTGAATGCCGGTT
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