CF509872

Overview
NameCF509872
Unique NameCF509872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length524
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 344.739 bits (883), Expect = 1.510e-94
Identity = 159/174 (91.38%), Postives = 167/174 (95.98%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATALFANSPFT+GKPNG++SMRSHIWTDTD +R GMLPFVFDDSFGFEQYVD+ALDVPMYFVYRKKKYIDC GM+FRDFLAGKLPC+PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ VLDMTADWT  ER
Sbjct:  250 DMIRKFRAGLALQPIATALFANSPFTDGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDFALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQRVLDMTADWTLEER 423
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 343.969 bits (881), Expect = 2.575e-94
Identity = 161/174 (92.53%), Postives = 166/174 (95.40%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDF+ GKL  +PG+ PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ VLDMT+DWT  ER
Sbjct:  264 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFMNGKLSPIPGDYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQTVLDMTSDWTAEER 437
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 5.736e-94
Identity = 159/174 (91.38%), Postives = 165/174 (94.83%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLS RSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKY+DC G+SFRDF+ GKLP +PGE PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ+VLDMT DWT  ER
Sbjct:  265 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDFMNGKLPPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEGSLQSVLDMTFDWTAEER 438
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 341.658 bits (875), Expect = 1.278e-93
Identity = 157/174 (90.23%), Postives = 164/174 (94.25%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATALFANSPFTEGKPNG+LSMRSHIWTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDC GM+FR FLAGKLPCLPGELP+ NDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYD+DSLQ +LD+TADWT  ER
Sbjct:  264 DMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER 437
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 4.856e-93
Identity = 156/174 (89.66%), Postives = 163/174 (93.68%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATALFANSPFTEGKPNG+LSMRSHIWTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYF YR  KY+DC GM+FR FLAGKLPCLPGELPT NDWENHLTTIFPEVRLKRY+EMRGADGGPWRRLCALPAFWVGLLYDED LQ+VLD+TADWT  ER
Sbjct:  256 DMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNGKYVDCTGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEVRLKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQSVLDLTADWTPAER 429
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 339.732 bits (870), Expect = 4.856e-93
Identity = 156/174 (89.66%), Postives = 165/174 (94.83%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFRAGLALQPIATA+FANSPF EGKPNGYLS+RSHIWTDTDNNR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDC GMSFRDF+ GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDE+SLQ++ DMT+DWT  ER
Sbjct:  238 DMIRKFRAGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEESLQSISDMTSDWTNEER 411
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 338.191 bits (866), Expect = 1.413e-92
Identity = 155/174 (89.08%), Postives = 164/174 (94.25%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFR GLALQPIATA+FANSPF EGKPNGYLS+RSHIWTDTDNNR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDC GMSFRDF+ GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDE+SLQ++ DMT+DWT  ER
Sbjct:  238 DMIRKFRTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEESLQSISDMTSDWTNEER 411
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 336.265 bits (861), Expect = 5.369e-92
Identity = 153/174 (87.93%), Postives = 163/174 (93.68%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKFR GLALQPIATA+FANSPF EGKPNGYLS+RSHIWTDTDNNR+GMLPFVFDDSFGFE+YVDYALD+PMYFVYR KKYIDC GMSFRDF+ GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALP FWVGLLYDE+SLQ++ DMT+DWT  ER
Sbjct:  234 DMIRKFRTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDIPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPVFWVGLLYDEESLQSISDMTSDWTNEER 407
[Close]
BLAST of CF509872 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 336.265 bits (861), Expect = 5.369e-92
Identity = 154/174 (88.51%), Postives = 163/174 (93.68%), Query Frame = 3 
Query:    3 DMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFLAGKLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQNVLDMTADWTTGER 524
            DMIRKF  GLALQPIATA+FANSPF EGKPNGYLS+RSHIWTDTDNNR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDC GMSFRDF+ GKLP  PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDE+SLQ++ DMT+DWT  ER
Sbjct:  234 DMIRKFHTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEESLQSISDMTSDWTNEER 407
The following BLAST results are available for this feature:
BLAST of CF509872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 9
Match NameE-valueIdentityDescription
GSH1_MEDTR1.510e-9491.38Glutamate--cysteine ligase, chloroplastic OS=Medic... [more]
GSH1_TOBAC2.575e-9492.53Glutamate--cysteine ligase, chloroplastic OS=Nicot... [more]
GSH1_SOLLC5.736e-9491.38Glutamate--cysteine ligase, chloroplastic OS=Solan... [more]
GSH1_ARATH1.278e-9390.23Glutamate--cysteine ligase, chloroplastic OS=Arabi... [more]
GSH1_BRAJU4.856e-9389.66Glutamate--cysteine ligase, chloroplastic OS=Brass... [more]
GSH1B_ORYSI4.856e-9389.66Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1B_ORYSJ1.413e-9289.08Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1A_ORYSJ5.369e-9287.93Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1A_ORYSI5.369e-9288.51Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-380 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-82_D10 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF509872 ID=CF509872; Name=CF509872; organism=Citrus sinensis; type=EST; length=524bp
CCGACATGATCCGCAAATTTCGTGCTGGTCTTGCTTTGCAGCCAATAGCA
ACAGCTCTATTTGCAAATTCACCATTTACCGAAGGAAAACCAAATGGCTA
TCTCAGCATGAGAAGCCACATCTGGACAGATACCGATAACAATCGTGCTG
GCATGCTTCCCTTTGTTTTTGATGACTCCTTTGGGTTTGAGCAATATGTT
GATTACGCCCTTGATGTTCCAATGTATTTTGTTTATCGCAAAAAGAAGTA
TATTGATTGTGCTGGGATGTCATTCAGGGACTTCTTGGCTGGAAAACTTC
CGTGCCTTCCTGGTGAATTGCCAACTCTTAATGATTGGGAAAATCATCTG
ACAACAATATTTCCCGAGGTTAGGTTGAAGAGATACTTGGAGATGAGAGG
CGCTGATGGAGGGCCTTGGAGGAGGTTATGTGCTTTGCCTGCATTTTGGG
TAGGTTTGTTATATGACGAGGACTCTCTCCAGAATGTTCTAGACATGACA
GCAGATTGGACTACAGGTGAAAGA
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