CF510028

Overview
NameCF510028
Unique NameCF510028
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length438
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9338 contig Csv1_Contig9338:216..653. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_METS3 (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) GN=mtaD PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.399e-18
Identity = 45/95 (47.37%), Postives = 59/95 (62.11%), Query Frame = 2 
Query:  149 GGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            G + +  D+I  IG   D         D++ID + +ILLPGFVNTH H S  L +G+ADD+ L +WL+D IWP E+N+T E  YI  LL  IELI
Sbjct:   24 GSLLIKNDKIAEIGTDID-----ESNVDKVIDAKGKILLPGFVNTHTHLSMTLFRGLADDLSLDSWLNDNIWPMEANLTSEYCYIGALLGAIELI 113
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_METBF (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=mtaD PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.399e-18
Identity = 44/115 (38.26%), Postives = 67/115 (58.26%), Query Frame = 2 
Query:   89 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            +I+ NA ++TMD +    +NG V +   +I  IG+        S+ AD +ID +  +++PG VNTH H +  L +G ADD+ L  WL   IWP E+ +T ED Y  +LL  +E+I
Sbjct:    4 IIVKNAYVMTMDPDEGDLKNGTVVIEDGKITEIGEKT------SESADTVIDAKHSVVMPGLVNTHTHAAMTLFRGYADDLQLADWLEGHIWPAEAKLTAEDVYKGSLLACLEMI 112
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_DESRM (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Desulfotomaculum reducens (strain MI-1) GN=mtaD PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.117e-18
Identity = 40/115 (34.78%), Postives = 64/115 (55.65%), Query Frame = 2 
Query:   89 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            +++  A I+TM+    +   G + +    I  +G        F    D++I+   Q+ +PGF+N H H +  L +G ADD+ LMTWL ++IWP+E  MT ED Y  T+L  +E+I
Sbjct:    4 LLIRGATILTMEGPEAIIETGELLIEDGWITHVGLPGSASGSFDM--DEVIEADGQVAMPGFINCHTHAAMTLLRGYADDLPLMTWLSEKIWPFEGRMTNEDIYWGTMLACLEMI 116
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_METMA (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina mazei GN=mtaD PE=3 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 2.638e-17
Identity = 42/115 (36.52%), Postives = 65/115 (56.52%), Query Frame = 2 
Query:   89 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            +I+ NA ++TMD ++   + G V +   +I  IG          + AD +ID +  +++PG VNTH H +  L +G ADD+ L  WL   IWP E+ +T ED Y  +LL  +E+I
Sbjct:    4 IIIKNAYVLTMDPDAGDIKKGTVVIEDGKITEIGVKT------KESADTVIDAKGSVVMPGLVNTHTHAAMTLFRGYADDLQLAEWLEKHIWPAEAQLTAEDVYRGSLLACLEMI 112
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_METAC (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina acetivorans GN=mtaD PE=3 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 2.638e-17
Identity = 41/115 (35.65%), Postives = 66/115 (57.39%), Query Frame = 2 
Query:   89 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            +I+ NA ++TMD ++   +NG V +   +I  IG++        + AD +ID +  +++PG  NTH H +  L +G ADD+ L  WL   IWP E+ +  ED Y  +LL  +E+I
Sbjct:    4 IIIKNAYVLTMDPDAGDLKNGTVVIEDGKITEIGENT------KENADTVIDAKGSVVMPGLANTHTHAAMTLFRGYADDLQLAEWLEKHIWPAEAQLKAEDVYKGSLLACLEMI 112
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_DESHY (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Desulfitobacterium hafniense (strain Y51) GN=mtaD PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.446e-17
Identity = 42/114 (36.84%), Postives = 64/114 (56.14%), Query Frame = 2 
Query:   92 ILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            IL  A+++ M      +  G + +  DRI  +G+       F  + DQIIDL   +++PG +NTH H +  + +  ADD+ LM WL  +IWP+E  M++ED Y  TLL   E+I
Sbjct:    4 ILIRAMVLPMTGPEDFYPEGEIGIENDRILFVGEKGSAPDSF--IPDQIIDLPEDVVMPGLINTHTHAAMTMLRSYADDLPLMPWLQTKIWPFEDKMSDEDIYWGTLLALGEMI 115
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_DESHD (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=mtaD PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.446e-17
Identity = 42/114 (36.84%), Postives = 64/114 (56.14%), Query Frame = 2 
Query:   92 ILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            IL  A+++ M      +  G + +  DRI  +G+       F  + DQIIDL   +++PG +NTH H +  + +  ADD+ LM WL  +IWP+E  M++ED Y  TLL   E+I
Sbjct:    4 ILIRAMVLPMTGPEDFYPEGEIGIENDRILFVGEKGSAPDSF--IPDQIIDLPEDVVMPGLINTHTHAAMTMLRSYADDLPLMPWLQTKIWPFEDKMSDEDIYWGTLLALGEMI 115
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_BACCR (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=mtaD PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.677e-17
Identity = 45/111 (40.54%), Postives = 66/111 (59.46%), Query Frame = 2 
Query:  101 NAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            NA IVTM++++ V  NG + V  D+I  + +S +    F    D++ID++ + +LPG VNTH H    L +GI DD+ L  WL  RIWP ES  T E +  ST L  +E++
Sbjct:    7 NATIVTMNEQNEVIENGYIIVENDQIIDV-KSGEFANDFE--VDEVIDMKGKWVLPGLVNTHTHVVMSLLRGIGDDMLLQPWLETRIWPLESQFTPELAVASTELGLLEMV 114
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_BACC7 (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Bacillus cereus (strain AH187) GN=mtaD PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.677e-17
Identity = 45/111 (40.54%), Postives = 66/111 (59.46%), Query Frame = 2 
Query:  101 NAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            NA IVTM++++ V  NG + V  D+I  + +S +    F    D++ID++ + +LPG VNTH H    L +GI DD+ L  WL  RIWP ES  T E +  ST L  +E++
Sbjct:    7 NATIVTMNEQNEVIENGYIIVENDQIIDV-KSGEFANDFE--VDEVIDMKGKWVLPGLVNTHTHVVMSLLRGIGDDMLLQPWLETRIWPLESQFTPELAVASTELGLLEMV 114
[Close]
BLAST of CF510028 vs. ExPASy Swiss-Prot
Match: MTAD_BACC2 (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Bacillus cereus (strain G9842) GN=mtaD PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.677e-17
Identity = 45/111 (40.54%), Postives = 66/111 (59.46%), Query Frame = 2 
Query:  101 NAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELI 433
            NA IVTM++++ V  NG + V  D+I  + +S +    F    D++ID++ + +LPG VNTH H    L +GI DD+ L  WL  RIWP ES  T E +  ST L  +E++
Sbjct:    7 NATIVTMNEQNEVIENGYIIVENDQIIDV-KSGEFANDFE--VDEVIDMKGKWVLPGLVNTHTHVVMSLLRGIGDDMLLQPWLETRIWPLESQFTPELAVASTELGLLEMV 114
The following BLAST results are available for this feature:
BLAST of CF510028 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 66
Match NameE-valueIdentityDescription
MTAD_METS31.399e-1847.375-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METBF1.399e-1838.265-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_DESRM3.117e-1834.785-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METMA2.638e-1736.525-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METAC2.638e-1735.655-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_DESHY3.446e-1736.845-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_DESHD3.446e-1736.845-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_BACCR7.677e-1740.545-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_BACC77.677e-1740.545-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_BACC27.677e-1740.545-methylthioadenosine/S-adenosylhomocysteine deami... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-543 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-84_C04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF510028 ID=CF510028; Name=CF510028; organism=Citrus sinensis; type=EST; length=438bp
GCACAGTCAAACTATAGACGAGCAAGAGATGGAGACAAACAGTAGCGGCG
GCGGCAGCAGCAGCGGAAGCTTAGGCTCAAGCTCAACGATGATACTTCAC
AACGCGGTGATTGTTACAATGGACAAAGAGAGCCGAGTATTTCGAAACGG
CGGCGTTTTCGTCGTACAAGACCGAATCAAAGCCATCGGTCAATCTGCCG
ATATTCTCCAACAATTCTCTCAAATGGCCGATCAAATCATCGATCTCCAA
AGCCAAATCTTACTTCCAGGATTTGTTAACACGCATGTGCACACATCTCA
ACAACTGGCGAAGGGGATAGCTGATGACGTTGATTTGATGACATGGTTGC
ATGATCGAATTTGGCCTTACGAATCTAACATGACTGAGGAGGATTCTTAC
ATCTCCACTTTACTGTGCGGAATTGAACTTATTCACTC
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