CF510035

Overview
NameCF510035
Unique NameCF510035
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length487
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX8_ARATH (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase OS=Arabidopsis thaliana GN=At3g26560 PE=1 SV=2)

HSP 1 Score: 273.092 bits (697), Expect = 4.656e-73
Identity = 132/161 (81.99%), Postives = 151/161 (93.79%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FSGYFFNCNIF+IPGRTFPV+I Y++Q  TDYLD+ALITVLQIH+ EPEGDIL+FLTGQEEID AC+SL E++K LGK+VPEL++LPVYSALPSE+QSRIF+P PPG RKVVVATNIAEASLTIDGI+YV+DPGFAKQNVYNPKQ L+SLVITPISQASA
Sbjct:  674 KFSGYFFNCNIFTIPGRTFPVEILYTKQPETDYLDAALITVLQIHLTEPEGDILVFLTGQEEIDSACQSLYERMKGLGKNVPELIILPVYSALPSEMQSRIFDPPPPGKRKVVVATNIAEASLTIDGIYYVVDPGFAKQNVYNPKQGLESLVITPISQASA 834
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX8_MOUSE (ATP-dependent RNA helicase DHX8 OS=Mus musculus GN=Dhx8 PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 4.368e-63
Identity = 113/161 (70.19%), Postives = 139/161 (86.34%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FS YF+   IF+IPGRT+PV+I Y+++  TDYLD++LITV+QIH+ EP GDIL+FLTGQEEID ACE L E++K+LG DVPEL++LPVYSALPSE+Q+RIF+PAPPG RKVV+ATNIAE SLTIDGI+YV+DPGF KQ VYN K  +D LV+TPISQA A
Sbjct:  748 KFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQA 908
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX8_HUMAN (ATP-dependent RNA helicase DHX8 OS=Homo sapiens GN=DHX8 PE=1 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 4.368e-63
Identity = 113/161 (70.19%), Postives = 139/161 (86.34%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FS YF+   IF+IPGRT+PV+I Y+++  TDYLD++LITV+QIH+ EP GDIL+FLTGQEEID ACE L E++K+LG DVPEL++LPVYSALPSE+Q+RIF+PAPPG RKVV+ATNIAE SLTIDGI+YV+DPGF KQ VYN K  +D LV+TPISQA A
Sbjct:  724 KFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQA 884
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: PRP22_SCHPO (Pre-mRNA-splicing factor ATP-dependent RNA helicase prp22 OS=Schizosaccharomyces pombe GN=prp22 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 5.705e-63
Identity = 113/161 (70.19%), Postives = 138/161 (85.71%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            RFS YF+ C IF+IPGR++PV+I Y++Q   DYLD+AL+TV+QIH+ E  GDIL+FLTGQEEID +CE L E+ K LG  +PELV+LPVYSALPSEIQSRIFEPAPPGGRKVV+ATNIAE SLTIDGI+YV+DPGF KQ+ ++PK  +DSL++TPISQA A
Sbjct:  670 RFSSYFYKCPIFTIPGRSYPVEIMYTKQPEADYLDAALMTVMQIHLSEGPGDILVFLTGQEEIDTSCEILYERSKMLGDSIPELVILPVYSALPSEIQSRIFEPAPPGGRKVVIATNIAETSLTIDGIYYVVDPGFVKQSCFDPKLGMDSLIVTPISQAQA 830
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX8_DICDI (ATP-dependent RNA helicase dhx8 OS=Dictyostelium discoideum GN=dhx8 PE=3 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 5.705e-63
Identity = 112/161 (69.57%), Postives = 138/161 (85.71%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FS YF N  +F IPGRTFPVDI Y++    DYLD++LITV+QIH+ EP GDILLFLTGQEEID AC+ L E++K+LG +VP+L++LPVYSALPSE+Q++IFEPAPPG RKVV+ATNIAE SLTIDGI+YVIDPGF+KQ  +NPK  +DSLV+ PISQA+A
Sbjct:  667 KFSKYFMNAQLFIIPGRTFPVDIRYTKDPEADYLDASLITVMQIHLSEPPGDILLFLTGQEEIDAACQILYERMKSLGSNVPDLIILPVYSALPSEMQTKIFEPAPPGSRKVVIATNIAETSLTIDGIYYVIDPGFSKQKCFNPKNGMDSLVVAPISQAAA 827
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: MOG5_CAEEL (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-5 OS=Caenorhabditis elegans GN=mog-5 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.405e-61
Identity = 110/161 (68.32%), Postives = 138/161 (85.71%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FS YF    IF+IPGRTFPV+I Y+R+  +DYL++A ITV+QIH+ EP GD+L+FLTGQEEID +CE L E++K++G DVPEL++LPVY ALPSE+Q+RIF+PAP G RKVV+ATNIAE SLTIDGIFYV+DPGF KQ +YNPK  +DSLV+TPISQA+A
Sbjct:  699 KFSEYFLEAPIFTIPGRTFPVEILYTREPESDYLEAAHITVMQIHLTEPPGDVLVFLTGQEEIDTSCEVLYERMKSMGPDVPELIILPVYGALPSEMQTRIFDPAPAGKRKVVIATNIAETSLTIDGIFYVVDPGFVKQKIYNPKSGMDSLVVTPISQAAA 859
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: PRP22_YEAST (Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 OS=Saccharomyces cerevisiae GN=PRP22 PE=1 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 2.173e-54
Identity = 98/161 (60.87%), Postives = 128/161 (79.50%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            +FS YF NC I +IPG+TFPV++ YS+    DY+++AL  V+ IH++E  GDIL+FLTGQEEID  CE L +++K LG  + EL++LPVYSALPSEIQS+IFEP P G RKVV ATNIAE S+TIDGI+YV+DPGFAK N+YN +  ++ L+++PISQA A
Sbjct:  642 KFSEYFLNCPIINIPGKTFPVEVLYSQTPQMDYIEAALDCVIDIHINEGPGDILVFLTGQEEIDSCCEILYDRVKTLGDSIGELLILPVYSALPSEIQSKIFEPTPKGSRKVVFATNIAETSITIDGIYYVVDPGFAKINIYNARAGIEQLIVSPISQAQA 802
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX16_PANTR (Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 OS=Pan troglodytes GN=DHX16 PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 2.034e-52
Identity = 96/161 (59.63%), Postives = 127/161 (78.88%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            RFS +F +  +F IPGR FPVDI Y++    DYL++ +++VLQIHV +P GDIL+FLTGQEEI+ ACE L ++ + LG  + EL+VLP+Y+ LPS++Q+RIF+P PPG RKVVVATNIAE SLTI+GI YV+DPGF KQ  YNP+  ++SL +TP S+ASA
Sbjct:  562 RFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHVTQPPGDILVFLTGQEEIEAACEMLQDRCRRLGSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGIIYVLDPGFCKQKSYNPRTGMESLTVTPCSKASA 722
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX16_HUMAN (Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 OS=Homo sapiens GN=DHX16 PE=1 SV=2)

HSP 1 Score: 204.527 bits (519), Expect = 2.034e-52
Identity = 96/161 (59.63%), Postives = 127/161 (78.88%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            RFS +F +  +F IPGR FPVDI Y++    DYL++ +++VLQIHV +P GDIL+FLTGQEEI+ ACE L ++ + LG  + EL+VLP+Y+ LPS++Q+RIF+P PPG RKVVVATNIAE SLTI+GI YV+DPGF KQ  YNP+  ++SL +TP S+ASA
Sbjct:  559 RFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHVTQPPGDILVFLTGQEEIEAACEMLQDRCRRLGSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGIIYVLDPGFCKQKSYNPRTGMESLTVTPCSKASA 719
[Close]
BLAST of CF510035 vs. ExPASy Swiss-Prot
Match: DHX16_DICDI (Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 OS=Dictyostelium discoideum GN=dhx16 PE=3 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 4.241e-50
Identity = 93/161 (57.76%), Postives = 123/161 (76.40%), Query Frame = 2 
Query:    2 RFSGYFFNCNIFSIPGRTFPVDINYSRQVVTDYLDSALITVLQIHVDEPEGDILLFLTGQEEIDFACESLCEKIKALGKDVPELVVLPVYSALPSEIQSRIFEPAPPGGRKVVVATNIAEASLTIDGIFYVIDPGFAKQNVYNPKQRLDSLVITPISQASA 484
            RFS YF     F+IPGR + V  +Y++    DYLD+A++TVLQIH+ EP GDIL+FLTGQEE+D A E L  + + LG  + EL++  +YS LP+++Q++IFEP PP  RKVV+ATNIAE SLTIDGI YVIDPGF KQ ++NP+  ++SLVITP+S+ASA
Sbjct:  626 RFSDYFDGAPTFNIPGRKYEVTTHYTQAPEADYLDAAVVTVLQIHITEPLGDILVFLTGQEEVDQAAEMLQTRTRGLGTKIKELIITRIYSTLPTDLQAKIFEPTPPNARKVVLATNIAETSLTIDGIIYVIDPGFCKQKMFNPRTGMESLVITPVSRASA 786
The following BLAST results are available for this feature:
BLAST of CF510035 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 71
Match NameE-valueIdentityDescription
DHX8_ARATH4.656e-7381.99Probable pre-mRNA-splicing factor ATP-dependent RN... [more]
DHX8_MOUSE4.368e-6370.19ATP-dependent RNA helicase DHX8 OS=Mus musculus GN... [more]
DHX8_HUMAN4.368e-6370.19ATP-dependent RNA helicase DHX8 OS=Homo sapiens GN... [more]
PRP22_SCHPO5.705e-6370.19Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
DHX8_DICDI5.705e-6369.57ATP-dependent RNA helicase dhx8 OS=Dictyostelium d... [more]
MOG5_CAEEL1.405e-6168.32Probable pre-mRNA-splicing factor ATP-dependent RN... [more]
PRP22_YEAST2.173e-5460.87Pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
DHX16_PANTR2.034e-5259.63Putative pre-mRNA-splicing factor ATP-dependent RN... [more]
DHX16_HUMAN2.034e-5259.63Putative pre-mRNA-splicing factor ATP-dependent RN... [more]
DHX16_DICDI4.241e-5057.76Putative pre-mRNA-splicing factor ATP-dependent RN... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-550 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-84_C12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF510035 ID=CF510035; Name=CF510035; organism=Citrus sinensis; type=EST; length=487bp
GAGATTTTCGGGATATTTCTTCAACTGCAACATCTTCAGTATTCCAGGGA
GAACATTTCCAGTTGATATAAACTACTCCAGACAGGTGGTAACTGATTAT
CTAGATTCAGCATTGATCACAGTCTTGCAGATCCACGTCGATGAACCTGA
AGGTGATATTCTTCTTTTCTTGACGGGTCAAGAGGAGATAGATTTTGCAT
GTGAGTCGCTTTGCGAGAAGATTAAAGCCCTGGGTAAAGATGTTCCTGAA
CTGGTTGTTTTACCAGTTTACAGTGCGCTGCCAAGTGAAATTCAATCAAG
AATATTTGAACCTGCCCCTCCAGGGGGGAGGAAAGTGGTTGTGGCCACTA
ACATTGCTGAGGCTTCTTTGACTATTGATGGGATATTTTATGTTATTGAT
CCCGGCTTTGCAAAGCAAAATGTCTATAATCCGAAGCAAAGGCTTGATTC
ACTGGTCATAACTCCAATTTCACAAGCATCAGCCCTG
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